OBJECTIVE To analyse structure and relationship of several andrographolide derivatives in multiple in vivo and in vitro angiogenesis assays,and to demonstrate a novel compound named AGL-2as a potential anti-angiogenes...OBJECTIVE To analyse structure and relationship of several andrographolide derivatives in multiple in vivo and in vitro angiogenesis assays,and to demonstrate a novel compound named AGL-2as a potential anti-angiogenesis agent.METHODS Human umbilical vein endothelial cells(HUVECs)in vitro and zebrafish(Danio rerio)in vivo models were used to screen and identify the anti-angiogenesis activities of six andrographolide derivatives;namely,AGL-1,AGL-2,AGS-72,AGS-72 a,AGS-79 and AGP-151.RESULTS AGL-2exhibited the strongest anti-angiogenic activity among all the derivatives in zebrafish model.Interestingly,another compound named AGS-72 showed stronger anti-angiogenic activity than AGL-2 in VEGF-induced HUVECs proliferation,migration,invasion and tube formation assays.In addition,AGL-2 was found to suppress the VEGF-induced VEGFR-2auto-phosphorylation and inhibit the activity of VEGFR-2 mediated signaling cascades in a dose-dependent manner.CONCLUSION AGL-2was demonstrated to be a promising anti-angiogenic agent among all the tested derivatives.The mechanism underlying the anti-angiogenic activity of AGL-2 probably involve VEGFR-2 signaling pathway.Even though,how some of chemical structure alterations result in discrepancy between in vivo and in vitro activities still remains to be resolved,this study shall provide new insight into how modification of the chemical structure of andrographolide affects this newly identified anti-angiogenesis activity.Meanwhile,AGL-2 can be exploited as a potential therapeutic agent for the treatment of angiogenesis-related diseases.展开更多
In recent years, in order to improve the speed of social development in our country, under such background, the development speed of various industries has been further improved. Industrial manufacturing and other fie...In recent years, in order to improve the speed of social development in our country, under such background, the development speed of various industries has been further improved. Industrial manufacturing and other fields are no exception, but development opportunities are often accompanied by many problems and challenges. The lack of talents is the main problem in the development of the times. As the main supply place of talents in our country, the teaching quality and teaching philosophy of colleges and universities have attracted wide attention from all walks of life, especially the hydraulic transmission course, which plays an extremely powerful role in promoting the development of various industries. Therefore, all universities and vocational colleges have speeded up the optimization and reform of their own education mechanism. This paper mainly analyzes the teaching methods of hydraulic transmission course, and puts forward the relevant application development direction combining with various modern teaching concepts and teaching schemes, hoping to teach students in accordance with their aptitude, improve the teaching level of hydraulic transmission course, and meet the talent gap in the development of our industry.展开更多
基金The project supported by Science and Technology Development Fund of Macao SAR(078/2011/A3)Research Committee of University of Macao〔MYRG138(Y1-Y4)-ICMS12-LMY〕
文摘OBJECTIVE To analyse structure and relationship of several andrographolide derivatives in multiple in vivo and in vitro angiogenesis assays,and to demonstrate a novel compound named AGL-2as a potential anti-angiogenesis agent.METHODS Human umbilical vein endothelial cells(HUVECs)in vitro and zebrafish(Danio rerio)in vivo models were used to screen and identify the anti-angiogenesis activities of six andrographolide derivatives;namely,AGL-1,AGL-2,AGS-72,AGS-72 a,AGS-79 and AGP-151.RESULTS AGL-2exhibited the strongest anti-angiogenic activity among all the derivatives in zebrafish model.Interestingly,another compound named AGS-72 showed stronger anti-angiogenic activity than AGL-2 in VEGF-induced HUVECs proliferation,migration,invasion and tube formation assays.In addition,AGL-2 was found to suppress the VEGF-induced VEGFR-2auto-phosphorylation and inhibit the activity of VEGFR-2 mediated signaling cascades in a dose-dependent manner.CONCLUSION AGL-2was demonstrated to be a promising anti-angiogenic agent among all the tested derivatives.The mechanism underlying the anti-angiogenic activity of AGL-2 probably involve VEGFR-2 signaling pathway.Even though,how some of chemical structure alterations result in discrepancy between in vivo and in vitro activities still remains to be resolved,this study shall provide new insight into how modification of the chemical structure of andrographolide affects this newly identified anti-angiogenesis activity.Meanwhile,AGL-2 can be exploited as a potential therapeutic agent for the treatment of angiogenesis-related diseases.
文摘In recent years, in order to improve the speed of social development in our country, under such background, the development speed of various industries has been further improved. Industrial manufacturing and other fields are no exception, but development opportunities are often accompanied by many problems and challenges. The lack of talents is the main problem in the development of the times. As the main supply place of talents in our country, the teaching quality and teaching philosophy of colleges and universities have attracted wide attention from all walks of life, especially the hydraulic transmission course, which plays an extremely powerful role in promoting the development of various industries. Therefore, all universities and vocational colleges have speeded up the optimization and reform of their own education mechanism. This paper mainly analyzes the teaching methods of hydraulic transmission course, and puts forward the relevant application development direction combining with various modern teaching concepts and teaching schemes, hoping to teach students in accordance with their aptitude, improve the teaching level of hydraulic transmission course, and meet the talent gap in the development of our industry.
