Objective:To construct a novel non-viral vector loaded with growth and differentiation factor-5(GDF-5) plasmid using chitosan,hyaluronic acid,and chondroitin sulfate for osteoarthritis (OA)gene therapy.Methods: Nano-m...Objective:To construct a novel non-viral vector loaded with growth and differentiation factor-5(GDF-5) plasmid using chitosan,hyaluronic acid,and chondroitin sulfate for osteoarthritis (OA)gene therapy.Methods: Nano-microspheres (NMPs)were prepared by mixing chitosan,hyaluronic acid,and chondreitin sulfate.GDF-5 plasmid was encapsulated in the NMPs through electrostatic adsorption.The basic characteristics of the NMPs were observed,and then they were co-cultured with chondrocytes to observe their effects on extracellular matrix (ECM) protein expression.Finally,NMPs loaded with GDF-5were inje.cted into the articular cavities of rabbits to observe their therapeutic effects on OA in vivo.Results:NMPs exhibited good physicochemical properties and low cytotoxicity.Their average diameter was (0.61±0.20)μm,and encapsulation efficiency was (38.19±0.36)%.According to Cell Counting Kit-8(CCK-8)assay,relative cell viability was 75%-99%when the total weight of NMPs was less than 560μg. Transfection efficiency was (62.0±2.1)% in a liposome group,and (60.0±1.8)% in the NMP group.There was no sig- nificant difference between the two groups (P>0.05).Immunohistochemical staining results suggested that NMPs can successfully transfect chondrocytes and stimulate ECM protein expression in vitro.Compared with the control groups, the NMP group significantly promoted the expression of chondrocyte ECM in vivo (P<0.05),as shown by analysis of the biochemical composition of chondrocyte ECM.When NMPs were injected into OA model rabbits,the expression of ECM proteins in chondrocytes was significantly promoted and the progression of OA was slowed down.Conclusions: Based on these data,we think that these NMPs with excellent physicochemical and biological properties could be promising non-viral vectors for OA gene therapy.展开更多
Thermoelectric(TE)materials can effectively achieve direct energy interconversions between heat and electrical energy(Seebeck,Peltier,and Thomson effects),showing wide applications in harvesting waste or low-grade hea...Thermoelectric(TE)materials can effectively achieve direct energy interconversions between heat and electrical energy(Seebeck,Peltier,and Thomson effects),showing wide applications in harvesting waste or low-grade heat,local cooling and sensing[1].展开更多
Helium gas is a scarce but important strategic resource,which is usually associated with natural gas.Presently,only one extra-large helium-rich gas field has been found in China:the Hetianhe Gas Field in the Tarim Bas...Helium gas is a scarce but important strategic resource,which is usually associated with natural gas.Presently,only one extra-large helium-rich gas field has been found in China:the Hetianhe Gas Field in the Tarim Basin.This paper reports a new example,the Dongsheng Gas Field(DGF)in the Ordos Basin.In this study,92 natural gas samples from the DGF were analyzed for helium content and isotope composition using isotope mass spectrometry.The natural gas samples were found to have an average helium content of 0.133%,with 65(70.7%)of the samples having a helium content of 0.1%or more.Based on the proven natural gas reserves of the DGF,the proven geological helium reserves were calculated to be 1.96×10^(8)m^(3),suggesting that it represents the first extra-large helium-rich natural gas reservoir to be hosted in tight sandstone in China.The^(3)He/^(4)He ratios of 5 natural gas samples from the DGF are within the range of 3.03×10^(−8)–3.44×10^(−8).Therefore,the helium in the gas field is thought to be of typical crustal origin and to have formed in the granitic basement that is rich in uranium and thorium.The accumulation of helium-rich natural gas was controlled by regional tectonic activities.Activity along the fault connecting the reservoir with the basement caused release of the helium gas,which entered the overlying strata along the fault and accumulated with conventional hydrocarbon gas.Based on the structural background and the distribution of helium source rocks in the Ordos Basin,the main helium source rocks with high exploration potential are located in deep strata within the north and middle parts of the basin.展开更多
基金Project supported by the National Natural Science Foundation of China(No.81201407)the Bureau of Science&Technology and Intellectual Property Nanchong City(Nos.NSMC20170203 and NSMC20170310)+1 种基金the Health and Family Planning Commission of Sichuan Province(No.17JP496)the Research Projects of North Sichuan Medical College(No.CBY13-A-ZD09),China
文摘Objective:To construct a novel non-viral vector loaded with growth and differentiation factor-5(GDF-5) plasmid using chitosan,hyaluronic acid,and chondroitin sulfate for osteoarthritis (OA)gene therapy.Methods: Nano-microspheres (NMPs)were prepared by mixing chitosan,hyaluronic acid,and chondreitin sulfate.GDF-5 plasmid was encapsulated in the NMPs through electrostatic adsorption.The basic characteristics of the NMPs were observed,and then they were co-cultured with chondrocytes to observe their effects on extracellular matrix (ECM) protein expression.Finally,NMPs loaded with GDF-5were inje.cted into the articular cavities of rabbits to observe their therapeutic effects on OA in vivo.Results:NMPs exhibited good physicochemical properties and low cytotoxicity.Their average diameter was (0.61±0.20)μm,and encapsulation efficiency was (38.19±0.36)%.According to Cell Counting Kit-8(CCK-8)assay,relative cell viability was 75%-99%when the total weight of NMPs was less than 560μg. Transfection efficiency was (62.0±2.1)% in a liposome group,and (60.0±1.8)% in the NMP group.There was no sig- nificant difference between the two groups (P>0.05).Immunohistochemical staining results suggested that NMPs can successfully transfect chondrocytes and stimulate ECM protein expression in vitro.Compared with the control groups, the NMP group significantly promoted the expression of chondrocyte ECM in vivo (P<0.05),as shown by analysis of the biochemical composition of chondrocyte ECM.When NMPs were injected into OA model rabbits,the expression of ECM proteins in chondrocytes was significantly promoted and the progression of OA was slowed down.Conclusions: Based on these data,we think that these NMPs with excellent physicochemical and biological properties could be promising non-viral vectors for OA gene therapy.
基金supported by the Guangdong Basic and Applied Basic Research Foundation(2025A1515012415).
文摘Thermoelectric(TE)materials can effectively achieve direct energy interconversions between heat and electrical energy(Seebeck,Peltier,and Thomson effects),showing wide applications in harvesting waste or low-grade heat,local cooling and sensing[1].
基金supported by the National Natural Science Foundation of China (Grant Nos. U20B6001, 42141021, 41625009, U19B6003, 41902160, 42172149 and 41872112)the China Postdoctoral Science Foundation (Grant Nos. 2019M650967, 2020T130721)the Strategic Priority Research Program of the Chinese Academy of Sciences (Grant No. XDA14010404)
文摘Helium gas is a scarce but important strategic resource,which is usually associated with natural gas.Presently,only one extra-large helium-rich gas field has been found in China:the Hetianhe Gas Field in the Tarim Basin.This paper reports a new example,the Dongsheng Gas Field(DGF)in the Ordos Basin.In this study,92 natural gas samples from the DGF were analyzed for helium content and isotope composition using isotope mass spectrometry.The natural gas samples were found to have an average helium content of 0.133%,with 65(70.7%)of the samples having a helium content of 0.1%or more.Based on the proven natural gas reserves of the DGF,the proven geological helium reserves were calculated to be 1.96×10^(8)m^(3),suggesting that it represents the first extra-large helium-rich natural gas reservoir to be hosted in tight sandstone in China.The^(3)He/^(4)He ratios of 5 natural gas samples from the DGF are within the range of 3.03×10^(−8)–3.44×10^(−8).Therefore,the helium in the gas field is thought to be of typical crustal origin and to have formed in the granitic basement that is rich in uranium and thorium.The accumulation of helium-rich natural gas was controlled by regional tectonic activities.Activity along the fault connecting the reservoir with the basement caused release of the helium gas,which entered the overlying strata along the fault and accumulated with conventional hydrocarbon gas.Based on the structural background and the distribution of helium source rocks in the Ordos Basin,the main helium source rocks with high exploration potential are located in deep strata within the north and middle parts of the basin.
