Objective:To investigate the neuroprotective efficacy of pomegranate and ellagic acid on the histopathological changes in the hippocampus of an aluminium chloride(AlCl<sub>3)induced rat model of Alzheimer’s dis...Objective:To investigate the neuroprotective efficacy of pomegranate and ellagic acid on the histopathological changes in the hippocampus of an aluminium chloride(AlCl<sub>3)induced rat model of Alzheimer’s disease.Methods:Sprague Dawley rats were divided into 4 groups(n=10each):GroupⅠ:serving as negative control;GroupⅡ,Alzheimer model,induced by administration of 17 mg/kg bw AlCl3;GroupⅢ,administered the same dose of AlCl3 with 50 mg/kg of pomegranate peel extract and GroupⅣ:administered ellagic acid(50 mg/kg)in addition to the same dose of AlCl3.The medication given to all groups continued for 28 days.All were given the compounds by gastric gavage.Radial arm maze test,hippocampus antioxidant markers,histopathology of the dentate gyrus,and CA3 of the hippocampus were evaluated.Results:Rats treated with pomegranate peel extract exposed to radial arm maze test showed less number of errors and reduced time needed to reach the criterion.There was an increase in the levels of glutathione,catalase,and total antioxidant capacity and decreased lipid peroxidation products.Histopathological features in dentate gyrus and CA3 as apoptosis and chromatolysis of pyramidal cells and granular layer,respectively,were decreased.Alzheimer characteristic neurofibrillary tangles and senile plaques were reduced.Treatment with ellagic acid ameliorated the pathological results but to a statistically lower level.Conclusions:Pomegranate peel extract alleviates memory deficit and restores antioxidant homeostasis following degenerative changes in the hippocampus induced by aluminium chloride in rats.展开更多
Objective: Aspirin resistance (AR) or poor response to aspirin is said to be high among subjects with diabetes and more so in patients with poor glycemic control. The aim of our study was to evaluate the prevalence of...Objective: Aspirin resistance (AR) or poor response to aspirin is said to be high among subjects with diabetes and more so in patients with poor glycemic control. The aim of our study was to evaluate the prevalence of AR among subjects with diabetes with moderate glycemic control and its association with inflammatory markers and cytokines. Design: This is a single-center open-label prospective clinical study. Methods: The study included 142 young male veterans (mean age of 49years) with Type 2 diabetes, with HbA1C of 7.7 ± 1.1. Urinary 11-dehydro-thromboxane beta-2 (11DhTx2) concentrations measured by immunoassay are the primary outcome measures. Results: Urinary 11DhTxB2 levels ≥ 1500 pg/mg of creatinine is considered as AR. Approximately 53% of subjects had AR. There are no significant differences in the clinical parameters, such as age, history of hypertension or BMI, waist to hip ratio;as well as biochemical parameters, such as HbA1C, lipid parameters or serum creatinine, between subjects with or without AR. Levels of 11DhTxB2 /cre correlated with history of CAD, abdominal fat content and IL-6 levels (p < 0.01) as well as abdominal fat content and IL-6 levels;duration of diabetes and history of CAD. Conclusions: Aspirin resistance is common in subjects with diabetes even with moderate control. Additional measures to improve aspirin response should be considered. Further studies with larger groups are needed to clarify the AR with various associated risk factors.展开更多
基金funded by Deanship of Scientific Research(DSR),King Abdulaziz University,Jeddah,under grant No.DF-658-2481441DSR technical and financial support
文摘Objective:To investigate the neuroprotective efficacy of pomegranate and ellagic acid on the histopathological changes in the hippocampus of an aluminium chloride(AlCl<sub>3)induced rat model of Alzheimer’s disease.Methods:Sprague Dawley rats were divided into 4 groups(n=10each):GroupⅠ:serving as negative control;GroupⅡ,Alzheimer model,induced by administration of 17 mg/kg bw AlCl3;GroupⅢ,administered the same dose of AlCl3 with 50 mg/kg of pomegranate peel extract and GroupⅣ:administered ellagic acid(50 mg/kg)in addition to the same dose of AlCl3.The medication given to all groups continued for 28 days.All were given the compounds by gastric gavage.Radial arm maze test,hippocampus antioxidant markers,histopathology of the dentate gyrus,and CA3 of the hippocampus were evaluated.Results:Rats treated with pomegranate peel extract exposed to radial arm maze test showed less number of errors and reduced time needed to reach the criterion.There was an increase in the levels of glutathione,catalase,and total antioxidant capacity and decreased lipid peroxidation products.Histopathological features in dentate gyrus and CA3 as apoptosis and chromatolysis of pyramidal cells and granular layer,respectively,were decreased.Alzheimer characteristic neurofibrillary tangles and senile plaques were reduced.Treatment with ellagic acid ameliorated the pathological results but to a statistically lower level.Conclusions:Pomegranate peel extract alleviates memory deficit and restores antioxidant homeostasis following degenerative changes in the hippocampus induced by aluminium chloride in rats.
文摘Objective: Aspirin resistance (AR) or poor response to aspirin is said to be high among subjects with diabetes and more so in patients with poor glycemic control. The aim of our study was to evaluate the prevalence of AR among subjects with diabetes with moderate glycemic control and its association with inflammatory markers and cytokines. Design: This is a single-center open-label prospective clinical study. Methods: The study included 142 young male veterans (mean age of 49years) with Type 2 diabetes, with HbA1C of 7.7 ± 1.1. Urinary 11-dehydro-thromboxane beta-2 (11DhTx2) concentrations measured by immunoassay are the primary outcome measures. Results: Urinary 11DhTxB2 levels ≥ 1500 pg/mg of creatinine is considered as AR. Approximately 53% of subjects had AR. There are no significant differences in the clinical parameters, such as age, history of hypertension or BMI, waist to hip ratio;as well as biochemical parameters, such as HbA1C, lipid parameters or serum creatinine, between subjects with or without AR. Levels of 11DhTxB2 /cre correlated with history of CAD, abdominal fat content and IL-6 levels (p < 0.01) as well as abdominal fat content and IL-6 levels;duration of diabetes and history of CAD. Conclusions: Aspirin resistance is common in subjects with diabetes even with moderate control. Additional measures to improve aspirin response should be considered. Further studies with larger groups are needed to clarify the AR with various associated risk factors.
