Traditional medicines leverage cultural wisdom,ancestral knowledge,and empirical observation to treat various diseases.This study examines traditional ecological knowledge(TEK)and evaluates the phytochemical compositi...Traditional medicines leverage cultural wisdom,ancestral knowledge,and empirical observation to treat various diseases.This study examines traditional ecological knowledge(TEK)and evaluates the phytochemical composition and antioxidant properties of fifteen traditional medicinal plants collected from the Nagar,Gilgit,and Skardu districts in Gilgit Baltistan,Pakistan.Utilizing standard phytochemical screening techniques,the study assessed total phenolic content(TPC),total flavonoid content(TFC),and antioxidant activities,such as radical scavenging activity(RSA)via the DPPH assay and total antioxidant capacity(TAC)using the FRAP assay,using two solvents(water and acetone).The highest TPC(283.21±1.04 mg GAE/100 g)and TFC(193.14±1.36 mg QE/100 g)were observed in the acetone extracts of Thymus linearis Benth.and Equisetum arvense L.respectively.Antioxidant activity analysis revealed solvent-specific variations,with water extract showing stronger radical scavenging activity(Artemisia absinthium L:87.47%)and acetone extract exhibiting higher total antioxidant capacity(Plantago lanceolata L:182.00 mg GAE/100 g).Multivariate analysis(PCA and HCA)highlighted Equisetum arvense L.and Rumex nepalensis Spreng.as the most bioactive species,clustering separately due to high TPC,TFC,and antioxidant properties.In contrast,plants like Delphinium brunonianum Royle.and Berberis lycium Royle.depicted lower bioactive compound concentrations.These findings confirm the potential of traditional medicinal plants,particularly those extracted with acetone,for pharmaceutical and nutraceutical applications.Future research should investigate the bioavailability and pharmacokinetics of these bioactive compounds to optimize their therapeutic potential.展开更多
基金the Ministry of Science and Tech-nology,Taiwan(MOST 109-2811-M-194-502,MOST 108-2811-M-194-510)the Ditmanson Medical Foundation Chia-Yi Christian Hospital Research Program(R114-23)。
文摘Traditional medicines leverage cultural wisdom,ancestral knowledge,and empirical observation to treat various diseases.This study examines traditional ecological knowledge(TEK)and evaluates the phytochemical composition and antioxidant properties of fifteen traditional medicinal plants collected from the Nagar,Gilgit,and Skardu districts in Gilgit Baltistan,Pakistan.Utilizing standard phytochemical screening techniques,the study assessed total phenolic content(TPC),total flavonoid content(TFC),and antioxidant activities,such as radical scavenging activity(RSA)via the DPPH assay and total antioxidant capacity(TAC)using the FRAP assay,using two solvents(water and acetone).The highest TPC(283.21±1.04 mg GAE/100 g)and TFC(193.14±1.36 mg QE/100 g)were observed in the acetone extracts of Thymus linearis Benth.and Equisetum arvense L.respectively.Antioxidant activity analysis revealed solvent-specific variations,with water extract showing stronger radical scavenging activity(Artemisia absinthium L:87.47%)and acetone extract exhibiting higher total antioxidant capacity(Plantago lanceolata L:182.00 mg GAE/100 g).Multivariate analysis(PCA and HCA)highlighted Equisetum arvense L.and Rumex nepalensis Spreng.as the most bioactive species,clustering separately due to high TPC,TFC,and antioxidant properties.In contrast,plants like Delphinium brunonianum Royle.and Berberis lycium Royle.depicted lower bioactive compound concentrations.These findings confirm the potential of traditional medicinal plants,particularly those extracted with acetone,for pharmaceutical and nutraceutical applications.Future research should investigate the bioavailability and pharmacokinetics of these bioactive compounds to optimize their therapeutic potential.
