BACKGROUND There is currently no effective treatment for osteoarthritis(OA),which is the most common joint disorder leading to disability.Although human umbilical cord mesenchymal stem cells(hUC-MSCs)are promising OA ...BACKGROUND There is currently no effective treatment for osteoarthritis(OA),which is the most common joint disorder leading to disability.Although human umbilical cord mesenchymal stem cells(hUC-MSCs)are promising OA treatments,their use is limited by the condition itself,and understanding of the underlying mechanisms of OA is lacking.AIM To explore the specific molecular mechanism by which hUC-MSC-derived exosomal miR-199a-3p improves OA.METHODS Sodium iodoacetate was injected into rat articulations to construct an animal model of OA.Interleukin(IL)-1βwas used to induce human chondrocytes(CHON-001)to construct an OA chondrocyte model.Exosomes in hUC-MSCs were isolated using Ribo™Exosome Isolation Reagent.Real-time reverse transcriptase-polymerase chain reaction and western blotting were used to detect the expression of related genes and proteins,and damage to CHON-001 cells and rat articular cartilage tissue was evaluated by enzyme-linked immunosorbent assay,terminal deoxynucleotidyl transferase-mediated deoxyuridine tripho-sphate-nick end labelling staining and hematoxylin and eosin staining.RESULTS hUC-MSC-derived exosomes(hUC-MSC-Exos)inhibited the expression of IL-1β-induced inflammatory cytokines,namely,IL-6,IL-8 and tumor necrosis factor-α.hUC-MSC-Exos also improved the viability but inhibited the apoptosis of CHON-001 cells,improved the pathological condition of articular cartilage tissue and alleviated the development of OA in vivo.Mechanistically,hUC-MSC-Exos downregulated the expression of mitogen-activated protein kinase 4 by delivering miR-199a-3p,thereby inhibiting the activation of the nuclear factor-kappaB signaling pathway,alleviating IL-1β-induced chondrocyte inflammation and apoptosis,and ultimately improving the development of OA.CONCLUSION hUC-MSC-derived exosomal miR-199a-3p alleviates OA by inhibiting the mitogen-activated protein kinase 4/nuclear factor-kappaB signaling pathway.The present findings suggest that miR-199a-3p delivery by hUC-MSCExos may be a novel strategy for the treatment of OA.展开更多
The consensus of the automotive industry and traffic management authorities is that autonomous vehicles must follow the same traffic laws as human drivers.Using formal or digital methods,natural language traffic rules...The consensus of the automotive industry and traffic management authorities is that autonomous vehicles must follow the same traffic laws as human drivers.Using formal or digital methods,natural language traffic rules can be translated into machine language and used by autonomous vehicles.In this paper,a translation flow is designed.Beyond the translation,a deeper examination is required,because the semantics of natural languages are rich and complex,and frequently contain hidden assumptions.The issue of how to ensure that digital rules are accurate and consistent with the original intent of the traffic rules they represent is both significant and unresolved.In response,we propose a method of formal verification that combines equivalence verification with model checking.Reasonable and reassuring digital traffic rules can be obtained by utilizing the proposed traffic rule digitization flow and verification method.In addition,we offer a number of simulation applications that employ digital traffic rules to assess vehicle violations.The experimental findings indicate that our digital rules utilizing metric temporal logic(MTL)can be easily incorporated into simulation platforms and autonomous driving systems(ADS).展开更多
Objective This study examined humanin expression in rat ovarian tissue,its cellular localization,and its correlation with rat age under physiological conditions.Methods A total of 40 Sprague-Dawley rats of various age...Objective This study examined humanin expression in rat ovarian tissue,its cellular localization,and its correlation with rat age under physiological conditions.Methods A total of 40 Sprague-Dawley rats of various ages(2,12,30,and 60 days old and 1 year old)were grouped by age.Immunofluorescence and immunohistochemical techniques were used to observe humanin expression and cellular location in the ovarian tissues of rats from each age group.In addition,Western blotting and Real-time quantitative reverse transcription PCR(qRT-PCR)techniques were used to measure humanin expression level in the ovarian tissues of rats from each age group.Results The immunofluorescence and immunohistochemical results confirmed that humanin was expressed in rat ovarian tissues.In addition,cellular localization analysis showed that humanin was expressed in the cytoplasm of oocytes,interstitial cells,granulosa cells and theca cells in all levels of follicles after the primary follicles,and in the corpus luteum.The qRT-PCR results revealed that the level of humanin expression in the ovarian tissues of 12-day-old rats was non-significantly higher than that in the ovarian tissues of 2-day-old rats(P>0.05),whereas the levels of humanin expression in the ovarian tissues of 30-day-old,60-day-old,and 1-year-old rats were significantly lower than that in the ovarian tissues of 2-day-old rats(P<0.05).The Western blotting results demonstrated that the levels of humanin protein expression in the ovarian tissues of 60-day-old and 1-year-old rats were significantly lower than those of 2-day-old rats(P<0.01),whereas there was no significant difference in the level of humanin protein expression between the ovarian tissues of 12-day-old and 30-day-old rats.Conclusion This study confirmed that humanin is expressed in the cytoplasm of various cells in rat ovarian tissues.Moreover,the level of humanin expression was highest in the ovarian tissues of 12-day-old rats,and it subsequently decreased with age.The changes in the expression of humanin in the ovary of rats at different ages will lay the foundation for the role of humanin in ovarian aging.The effect of humanin on ovarian function is worthy of further study in the future.展开更多
Objective:To study the correlation of myosin light chain kinase (MLCK) and fibroblast activation protein (FAP) expression with uterine fibroid cell proliferation and invasion. Methods:Uterine fibroids samples and norm...Objective:To study the correlation of myosin light chain kinase (MLCK) and fibroblast activation protein (FAP) expression with uterine fibroid cell proliferation and invasion. Methods:Uterine fibroids samples and normal uterine muscle samples next to fibroids that were surgically removed in Wuhan Red Cross Hospital between May 2014 and January 2017 were chosen, fluorescence quantitative PCR kits were used to deterct MLCK and FAP mRNA expression, and enzyme-linked immunosorbent assay kits were used to determine proliferation and invasion gene protein expression.Results: MLCK and FAP mRNA expression in uterine fibroids samples were significantly higher than those in normal uterine muscle samples, and Survivin, Livin, Bcl-2, Snail, N-cadherin and MMP2 protein expression were significantly higher than those in normal uterine muscle samples;Survivin, Livin, Bcl-2, Snail, N-cadherin and MMP2 protein expression in uterine fibroids samples with high MLCK and FAP expression were significantly higher than those in uterine fibroids samples with low MLCK and FAP expression.Conclusion: Highly expressed MLCK and FAP in uterine fibroids can promote the proliferation and invasion of uterine fibroids.展开更多
Objective: To study the effect of folic acid and vitamin supplement on endothelial injury and placental blood perfusion in patients with hypertensive disorder complicating pregnancy. Methods: A total of 62 patients wh...Objective: To study the effect of folic acid and vitamin supplement on endothelial injury and placental blood perfusion in patients with hypertensive disorder complicating pregnancy. Methods: A total of 62 patients who were diagnosed with preeclampsia in Wuhan Red Cross hospital between March 2015 and February 2017 were selected as the research subjects and randomly divided into two groups, the observation group received folic acid, vitamin and conventional symptomatic treatment, and control group accepted conventional symptomatic treatment. Serum contents of endothelial injury markers and oxidative stress indexes were detected before treatment and 2 weeks after treatment;the expression of apoptosis genes in the placenta were detected after delivery. Results: 2 weeks after treatment, serum ET-1, tTG, sFlt-1, sEng, MPO, IMA, MDA and AOPP levels of both groups of patients were significantly lower than those before treatment, and serum ET-1, tTG, sFlt-1, sEng, MPO, IMA, MDA and AOPP levels of observation group were significantly lower than those of control group;after delivery, Fas, Bax and Caspase-3 mRNA expression levels in placenta tissue of observation group were significantly lower than those of control group while Bcl-2, Survivin and Livin mRNA expression levels were significantly higher than those of control group. Conclusion:Folic acid and vitamin supplement can significantly improve the endothelial injury and placental blood perfusion in patients with hypertensive disorder complicating pregnancy.展开更多
Many enterprises and personals are inclining to outsource their data to public clouds, but security and privacy are two critical problems cannot be ignored. The door of cloud provider may be broken, and the data may a...Many enterprises and personals are inclining to outsource their data to public clouds, but security and privacy are two critical problems cannot be ignored. The door of cloud provider may be broken, and the data may also be dug into by providers to find valuable information. In this paper, a secure and efficient storage file (SES FS) system is proposed to distribute files in several clouds and allows users to search the files securely and efficiently. In the proposed system, keywords were transformed into integers and secretly shared in a defined finite field, then the shares were mapped to random numbers in specified random domain in each cloud. Files were encrypted with distinct secret key and scattered within different clouds. Information about keyword/file was secretly shared among cloud providers. Legal users can search in the clouds to find correct encrypted files and reconstruct corresponding secret key. No adversary can find or detect the real file information even they can collude all the servers. Manipulation on shares by one or more clouds can be detected with high probability. The system can also detect malicious servers through introduced virtual points. One interesting property for the scheme is that new keywords can be added easily, which is difficult and usually not efficient for many searchable symmetric encryption systems. Detailed experimental result shows, with tolerable uploading delay, the scheme exhibits excellent performance on data retrieving aspect.展开更多
The encryption of user data is crucial when employing electronic health record services to guarantee the security of the data stored on cloud servers.Attribute-based encryption(ABE)scheme is considered a powerful encr...The encryption of user data is crucial when employing electronic health record services to guarantee the security of the data stored on cloud servers.Attribute-based encryption(ABE)scheme is considered a powerful encryption technique that ofers fexible and fne-grained access control capabilities.Further,the multi-user collaborative access ABE scheme additionally supports users to acquire access authorization through collaborative works.However,the existing multi-user collaborative access ABE schemes do not consider the diferent weights of collaboration users.Therefore,using these schemes for weighted multi-user collaborative access results in redundant attributes,which inevitably reduces the efciency of the ABE scheme.This paper proposes a revocable and verifable weighted attribute-based encryption with collaborative access scheme(RVWABE-CA),which can provide efcient weighted multi-user collaborative access,user revocation,and data integrity verifcation,as the fundamental cornerstone for establishing a robust framework to facilitate secure sharing of electronic health records in a public cloud environment.In detail,this scheme employs a novel weighted access tree to eliminate redundant attributes,utilizes encryption version information to control user revocation,and establishes Merkle Hash Tree for data integrity verifcation.We prove that our scheme is resistant against chosen plaintext attack.The experimental results demonstrate that our scheme has signifcant computational efciency advantages compared to related works,without increasing storage or communication overhead.Therefore,the RVWABE-CA scheme can provide an efcient and fexible weighted collaborative access control and user revocation mechanism as well as data integrity verifcation for electronic health record systems.展开更多
基金Supported by Basic Research Plan of Yunnan Province,No.202201AT070059National Natural Science Foundation of China,No.81760407Science and Technology Talent and Platform Plan of Yunnan Provincial Department of Science and Technology,No.202205AC160066.
文摘BACKGROUND There is currently no effective treatment for osteoarthritis(OA),which is the most common joint disorder leading to disability.Although human umbilical cord mesenchymal stem cells(hUC-MSCs)are promising OA treatments,their use is limited by the condition itself,and understanding of the underlying mechanisms of OA is lacking.AIM To explore the specific molecular mechanism by which hUC-MSC-derived exosomal miR-199a-3p improves OA.METHODS Sodium iodoacetate was injected into rat articulations to construct an animal model of OA.Interleukin(IL)-1βwas used to induce human chondrocytes(CHON-001)to construct an OA chondrocyte model.Exosomes in hUC-MSCs were isolated using Ribo™Exosome Isolation Reagent.Real-time reverse transcriptase-polymerase chain reaction and western blotting were used to detect the expression of related genes and proteins,and damage to CHON-001 cells and rat articular cartilage tissue was evaluated by enzyme-linked immunosorbent assay,terminal deoxynucleotidyl transferase-mediated deoxyuridine tripho-sphate-nick end labelling staining and hematoxylin and eosin staining.RESULTS hUC-MSC-derived exosomes(hUC-MSC-Exos)inhibited the expression of IL-1β-induced inflammatory cytokines,namely,IL-6,IL-8 and tumor necrosis factor-α.hUC-MSC-Exos also improved the viability but inhibited the apoptosis of CHON-001 cells,improved the pathological condition of articular cartilage tissue and alleviated the development of OA in vivo.Mechanistically,hUC-MSC-Exos downregulated the expression of mitogen-activated protein kinase 4 by delivering miR-199a-3p,thereby inhibiting the activation of the nuclear factor-kappaB signaling pathway,alleviating IL-1β-induced chondrocyte inflammation and apoptosis,and ultimately improving the development of OA.CONCLUSION hUC-MSC-derived exosomal miR-199a-3p alleviates OA by inhibiting the mitogen-activated protein kinase 4/nuclear factor-kappaB signaling pathway.The present findings suggest that miR-199a-3p delivery by hUC-MSCExos may be a novel strategy for the treatment of OA.
文摘The consensus of the automotive industry and traffic management authorities is that autonomous vehicles must follow the same traffic laws as human drivers.Using formal or digital methods,natural language traffic rules can be translated into machine language and used by autonomous vehicles.In this paper,a translation flow is designed.Beyond the translation,a deeper examination is required,because the semantics of natural languages are rich and complex,and frequently contain hidden assumptions.The issue of how to ensure that digital rules are accurate and consistent with the original intent of the traffic rules they represent is both significant and unresolved.In response,we propose a method of formal verification that combines equivalence verification with model checking.Reasonable and reassuring digital traffic rules can be obtained by utilizing the proposed traffic rule digitization flow and verification method.In addition,we offer a number of simulation applications that employ digital traffic rules to assess vehicle violations.The experimental findings indicate that our digital rules utilizing metric temporal logic(MTL)can be easily incorporated into simulation platforms and autonomous driving systems(ADS).
文摘Objective This study examined humanin expression in rat ovarian tissue,its cellular localization,and its correlation with rat age under physiological conditions.Methods A total of 40 Sprague-Dawley rats of various ages(2,12,30,and 60 days old and 1 year old)were grouped by age.Immunofluorescence and immunohistochemical techniques were used to observe humanin expression and cellular location in the ovarian tissues of rats from each age group.In addition,Western blotting and Real-time quantitative reverse transcription PCR(qRT-PCR)techniques were used to measure humanin expression level in the ovarian tissues of rats from each age group.Results The immunofluorescence and immunohistochemical results confirmed that humanin was expressed in rat ovarian tissues.In addition,cellular localization analysis showed that humanin was expressed in the cytoplasm of oocytes,interstitial cells,granulosa cells and theca cells in all levels of follicles after the primary follicles,and in the corpus luteum.The qRT-PCR results revealed that the level of humanin expression in the ovarian tissues of 12-day-old rats was non-significantly higher than that in the ovarian tissues of 2-day-old rats(P>0.05),whereas the levels of humanin expression in the ovarian tissues of 30-day-old,60-day-old,and 1-year-old rats were significantly lower than that in the ovarian tissues of 2-day-old rats(P<0.05).The Western blotting results demonstrated that the levels of humanin protein expression in the ovarian tissues of 60-day-old and 1-year-old rats were significantly lower than those of 2-day-old rats(P<0.01),whereas there was no significant difference in the level of humanin protein expression between the ovarian tissues of 12-day-old and 30-day-old rats.Conclusion This study confirmed that humanin is expressed in the cytoplasm of various cells in rat ovarian tissues.Moreover,the level of humanin expression was highest in the ovarian tissues of 12-day-old rats,and it subsequently decreased with age.The changes in the expression of humanin in the ovary of rats at different ages will lay the foundation for the role of humanin in ovarian aging.The effect of humanin on ovarian function is worthy of further study in the future.
文摘Objective:To study the correlation of myosin light chain kinase (MLCK) and fibroblast activation protein (FAP) expression with uterine fibroid cell proliferation and invasion. Methods:Uterine fibroids samples and normal uterine muscle samples next to fibroids that were surgically removed in Wuhan Red Cross Hospital between May 2014 and January 2017 were chosen, fluorescence quantitative PCR kits were used to deterct MLCK and FAP mRNA expression, and enzyme-linked immunosorbent assay kits were used to determine proliferation and invasion gene protein expression.Results: MLCK and FAP mRNA expression in uterine fibroids samples were significantly higher than those in normal uterine muscle samples, and Survivin, Livin, Bcl-2, Snail, N-cadherin and MMP2 protein expression were significantly higher than those in normal uterine muscle samples;Survivin, Livin, Bcl-2, Snail, N-cadherin and MMP2 protein expression in uterine fibroids samples with high MLCK and FAP expression were significantly higher than those in uterine fibroids samples with low MLCK and FAP expression.Conclusion: Highly expressed MLCK and FAP in uterine fibroids can promote the proliferation and invasion of uterine fibroids.
文摘Objective: To study the effect of folic acid and vitamin supplement on endothelial injury and placental blood perfusion in patients with hypertensive disorder complicating pregnancy. Methods: A total of 62 patients who were diagnosed with preeclampsia in Wuhan Red Cross hospital between March 2015 and February 2017 were selected as the research subjects and randomly divided into two groups, the observation group received folic acid, vitamin and conventional symptomatic treatment, and control group accepted conventional symptomatic treatment. Serum contents of endothelial injury markers and oxidative stress indexes were detected before treatment and 2 weeks after treatment;the expression of apoptosis genes in the placenta were detected after delivery. Results: 2 weeks after treatment, serum ET-1, tTG, sFlt-1, sEng, MPO, IMA, MDA and AOPP levels of both groups of patients were significantly lower than those before treatment, and serum ET-1, tTG, sFlt-1, sEng, MPO, IMA, MDA and AOPP levels of observation group were significantly lower than those of control group;after delivery, Fas, Bax and Caspase-3 mRNA expression levels in placenta tissue of observation group were significantly lower than those of control group while Bcl-2, Survivin and Livin mRNA expression levels were significantly higher than those of control group. Conclusion:Folic acid and vitamin supplement can significantly improve the endothelial injury and placental blood perfusion in patients with hypertensive disorder complicating pregnancy.
基金Demonstration on the Construction of Guangdong Survey and Geomatics Industry Technology Innovation Alliance (2017B090907030)The Demonstration of Big Data Application for Land Resource Management and Service (2015B010110006)+3 种基金Qiong Huang is supported by Guangdong Natural Science Funds for Distinguished Young Scholar (No. 2014A030306021)Guangdong Program for Special Support of Top-notch Young Professionals (No. 2015TQ01X796)Pearl River Nova Program of Guangzhou (No. 201610010037)and the National Natural Science Foundation of China (Nos. 61472146, 61672242).
文摘Many enterprises and personals are inclining to outsource their data to public clouds, but security and privacy are two critical problems cannot be ignored. The door of cloud provider may be broken, and the data may also be dug into by providers to find valuable information. In this paper, a secure and efficient storage file (SES FS) system is proposed to distribute files in several clouds and allows users to search the files securely and efficiently. In the proposed system, keywords were transformed into integers and secretly shared in a defined finite field, then the shares were mapped to random numbers in specified random domain in each cloud. Files were encrypted with distinct secret key and scattered within different clouds. Information about keyword/file was secretly shared among cloud providers. Legal users can search in the clouds to find correct encrypted files and reconstruct corresponding secret key. No adversary can find or detect the real file information even they can collude all the servers. Manipulation on shares by one or more clouds can be detected with high probability. The system can also detect malicious servers through introduced virtual points. One interesting property for the scheme is that new keywords can be added easily, which is difficult and usually not efficient for many searchable symmetric encryption systems. Detailed experimental result shows, with tolerable uploading delay, the scheme exhibits excellent performance on data retrieving aspect.
基金supported in part by the National Natural Science Foundation of China under Grant 61872409,Grant 61872152 and Grant 62272174in part by Guangdong Basic and Applied Basic Research Foundation under Grant 2020A1515010751+2 种基金in part by the Guangdong Major Project of Basic and Applied Basic Research under Grant 2019B030302008in part by the Science and Technology Program of Guangzhou under Grant 201902010081in part by Guangdong Basic and Applied Basic Research Foundation under Grant 2023A1515011194.
文摘The encryption of user data is crucial when employing electronic health record services to guarantee the security of the data stored on cloud servers.Attribute-based encryption(ABE)scheme is considered a powerful encryption technique that ofers fexible and fne-grained access control capabilities.Further,the multi-user collaborative access ABE scheme additionally supports users to acquire access authorization through collaborative works.However,the existing multi-user collaborative access ABE schemes do not consider the diferent weights of collaboration users.Therefore,using these schemes for weighted multi-user collaborative access results in redundant attributes,which inevitably reduces the efciency of the ABE scheme.This paper proposes a revocable and verifable weighted attribute-based encryption with collaborative access scheme(RVWABE-CA),which can provide efcient weighted multi-user collaborative access,user revocation,and data integrity verifcation,as the fundamental cornerstone for establishing a robust framework to facilitate secure sharing of electronic health records in a public cloud environment.In detail,this scheme employs a novel weighted access tree to eliminate redundant attributes,utilizes encryption version information to control user revocation,and establishes Merkle Hash Tree for data integrity verifcation.We prove that our scheme is resistant against chosen plaintext attack.The experimental results demonstrate that our scheme has signifcant computational efciency advantages compared to related works,without increasing storage or communication overhead.Therefore,the RVWABE-CA scheme can provide an efcient and fexible weighted collaborative access control and user revocation mechanism as well as data integrity verifcation for electronic health record systems.
