Objective:To assess the antibacterial activity of eight Iranian plant extracts against Staphylococcus aureus(S.aureus) strains which were isolated from infected patients.Methods: The studied strains were isolated from...Objective:To assess the antibacterial activity of eight Iranian plant extracts against Staphylococcus aureus(S.aureus) strains which were isolated from infected patients.Methods: The studied strains were isolated from urine,stool,blood and wound of infected patients and identified by biochemical tests.In further,the antibacterial activity of 8 ethanolic local plant extracts including Querqus brantii(Q.brantii),Ziziphus spina-christi(Z.spina-christi),Peganum harmala(P.harmala),Oliveira decumbens(O.decumbens),Galium tricornutum(G.tricornutum), Vitex pseudo negundo(Vi.pseudo negundo),Salvia officinalis(S.officinalis),Vaccaria pyramidata (V.pyramidata) were then evaluated using agar disc diffusion method.Results:A total of 9 isolates were isolated and identified as S.aureus.Antibacterial profile of the strains showed that the strains were resistant to methicillin and cefixime.The highest antibacterial activity against the studied strains were belong to Q.brantii,P.harmala,Z.spina-christi and O.decumbens vent extracts with 11-40 mm,15-28 mm,8-26 mm and 10-20 mm of diameters,respectively. Intermediate antibacterial activity was exhibited by G.tricornutum and Vi.pseudo negundo against some of the studied strains with 7-20 mm and 7-13 mm of diameters,respectively. Noteworthy,out of 9 studied strains;6 and 2 of them were resistant to the G.tricornutum and Vi. pseudo negundo extracts,respectively.The S.officinalis and Va.pyramidata,however,showed no antibacterial activity against the studied strains.Conclusions:The Q.brantii,P.harmala,Z. spina-christi and O.decumbens extracts were shown to possess strong antibacterial activity against methicillin and cefixime resistant S.aureus strains and can be considered as the promising natural antibiotics for treating the studied strains.展开更多
Brucellosis, a zoonosis caused by four species of brucella, has a high morbidity. Brucella melitensis is the main causative agent of brucellosis in both human and small ruminants. As an alternative to conventional ant...Brucellosis, a zoonosis caused by four species of brucella, has a high morbidity. Brucella melitensis is the main causative agent of brucellosis in both human and small ruminants. As an alternative to conventional antibiotics, medicinal plants are valuable resources for new agents against antibiotic-resistant strains. The aim of this study was to investigate the usage of native plants for brucellosis treatment. For this purpose, the anti-brucella activities of ethanolic and methanolic extracts of Salvia sclarea, Oliveria decumbens, Ferulago angulata, Vitex pseudo-negundo, Teucrium polium, Plantago ovata, Cordia myxa, and Crocus sativus were assessed. The activity against a resistant Br. melitensis strain was determined by disc diffusion method at various concentrations from 50-400 mg/ml. Antibiotic discs were also used as a control. Among the evaluated herbs, six plant (Salvia sclarea, Oliveria decumbens, Ferulago angulata, Vitex pseudo-negundo, Teucrium polium, and Crocus sativus) showed anti-brucella activity. Oliveria de- cumbens was chosen as the most effective plant for further studies. A tested isolate exhibited resistance to tetracycline, nafcillin, oxacillin, methicillin, and colistin. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values for Oliveria decumbens against resistant Br. melitensis were the same (5 mg/ml), and for gentamicin they were both 2 mg/ml. Time-kill kinetics for a methanolic extract of Oliveria decumbens was 7 h whereas for an ethanolic extract it was 28 h. Also, Oliveria decumbens extracts showed a synergistic effect in combination with doxycycline and tetracycline. In general, the similar values of MIC and MBC for Oliveria decumbens suggest that these extracts could act as bactericidal agents against Br. melitensis. In addition to Oliveria decumbens, Crocus sativus and Salvia sclarea also had good anti-brucella activity and these should be considered for further study.展开更多
Objective:To explore the antibacterial effect of the alcoholic extracts of aerial parts of Teucrium polium,native in Iran on some pathogenic bacteria.Methods:Antibacterial activity of ethanolic extract(50 to 400 mg/mL...Objective:To explore the antibacterial effect of the alcoholic extracts of aerial parts of Teucrium polium,native in Iran on some pathogenic bacteria.Methods:Antibacterial activity of ethanolic extract(50 to 400 mg/mL) and methanolic extract(400 and 600 mg/mL) was evaluated by disc diffusion method.Results:The ethanolic extract results showed that Bacillus anthracis was the most sensitive species,while Escherichia coli and Proteus mirabilis were more resistant than others.In the case of the methanolic extract,Bordetella bronchiseptica was the most sensitive and Proteus mirabilis and Arcanobacterium pyogenes were the most resistant species.The hydroalcoholic extract of Teucrium polium had a relatively satisfactory effect on Salmonella typhi. The minimal inhibitory concentration(MIC) of Staphylococcus aureus and Salmonella typhi was 40 mg/mL and Bordetella bronchiseptica and Bacillus anthracis was 10 mg/mL.The minimal bactericidal concentration(MBC) against Bacillus anthracis was 10 mg/mL while against other species were not found(】200 mg/mL).The methanolic extract had also synergistic effect with methicillin,vancomycin against Staphylococcus aureus and with novobiocin against Salmonella typhi.Conclusions:These results suggest that this plant contains relatively good antibacterial activity and it can be used as a source of antiseptic compounds for medicinal uses.展开更多
文摘Objective:To assess the antibacterial activity of eight Iranian plant extracts against Staphylococcus aureus(S.aureus) strains which were isolated from infected patients.Methods: The studied strains were isolated from urine,stool,blood and wound of infected patients and identified by biochemical tests.In further,the antibacterial activity of 8 ethanolic local plant extracts including Querqus brantii(Q.brantii),Ziziphus spina-christi(Z.spina-christi),Peganum harmala(P.harmala),Oliveira decumbens(O.decumbens),Galium tricornutum(G.tricornutum), Vitex pseudo negundo(Vi.pseudo negundo),Salvia officinalis(S.officinalis),Vaccaria pyramidata (V.pyramidata) were then evaluated using agar disc diffusion method.Results:A total of 9 isolates were isolated and identified as S.aureus.Antibacterial profile of the strains showed that the strains were resistant to methicillin and cefixime.The highest antibacterial activity against the studied strains were belong to Q.brantii,P.harmala,Z.spina-christi and O.decumbens vent extracts with 11-40 mm,15-28 mm,8-26 mm and 10-20 mm of diameters,respectively. Intermediate antibacterial activity was exhibited by G.tricornutum and Vi.pseudo negundo against some of the studied strains with 7-20 mm and 7-13 mm of diameters,respectively. Noteworthy,out of 9 studied strains;6 and 2 of them were resistant to the G.tricornutum and Vi. pseudo negundo extracts,respectively.The S.officinalis and Va.pyramidata,however,showed no antibacterial activity against the studied strains.Conclusions:The Q.brantii,P.harmala,Z. spina-christi and O.decumbens extracts were shown to possess strong antibacterial activity against methicillin and cefixime resistant S.aureus strains and can be considered as the promising natural antibiotics for treating the studied strains.
基金Project supported by the finance grant provided by the vice chan-cellor of research of Shahid Chamran University,Ahvaz,Iran
文摘Brucellosis, a zoonosis caused by four species of brucella, has a high morbidity. Brucella melitensis is the main causative agent of brucellosis in both human and small ruminants. As an alternative to conventional antibiotics, medicinal plants are valuable resources for new agents against antibiotic-resistant strains. The aim of this study was to investigate the usage of native plants for brucellosis treatment. For this purpose, the anti-brucella activities of ethanolic and methanolic extracts of Salvia sclarea, Oliveria decumbens, Ferulago angulata, Vitex pseudo-negundo, Teucrium polium, Plantago ovata, Cordia myxa, and Crocus sativus were assessed. The activity against a resistant Br. melitensis strain was determined by disc diffusion method at various concentrations from 50-400 mg/ml. Antibiotic discs were also used as a control. Among the evaluated herbs, six plant (Salvia sclarea, Oliveria decumbens, Ferulago angulata, Vitex pseudo-negundo, Teucrium polium, and Crocus sativus) showed anti-brucella activity. Oliveria de- cumbens was chosen as the most effective plant for further studies. A tested isolate exhibited resistance to tetracycline, nafcillin, oxacillin, methicillin, and colistin. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values for Oliveria decumbens against resistant Br. melitensis were the same (5 mg/ml), and for gentamicin they were both 2 mg/ml. Time-kill kinetics for a methanolic extract of Oliveria decumbens was 7 h whereas for an ethanolic extract it was 28 h. Also, Oliveria decumbens extracts showed a synergistic effect in combination with doxycycline and tetracycline. In general, the similar values of MIC and MBC for Oliveria decumbens suggest that these extracts could act as bactericidal agents against Br. melitensis. In addition to Oliveria decumbens, Crocus sativus and Salvia sclarea also had good anti-brucella activity and these should be considered for further study.
文摘Objective:To explore the antibacterial effect of the alcoholic extracts of aerial parts of Teucrium polium,native in Iran on some pathogenic bacteria.Methods:Antibacterial activity of ethanolic extract(50 to 400 mg/mL) and methanolic extract(400 and 600 mg/mL) was evaluated by disc diffusion method.Results:The ethanolic extract results showed that Bacillus anthracis was the most sensitive species,while Escherichia coli and Proteus mirabilis were more resistant than others.In the case of the methanolic extract,Bordetella bronchiseptica was the most sensitive and Proteus mirabilis and Arcanobacterium pyogenes were the most resistant species.The hydroalcoholic extract of Teucrium polium had a relatively satisfactory effect on Salmonella typhi. The minimal inhibitory concentration(MIC) of Staphylococcus aureus and Salmonella typhi was 40 mg/mL and Bordetella bronchiseptica and Bacillus anthracis was 10 mg/mL.The minimal bactericidal concentration(MBC) against Bacillus anthracis was 10 mg/mL while against other species were not found(】200 mg/mL).The methanolic extract had also synergistic effect with methicillin,vancomycin against Staphylococcus aureus and with novobiocin against Salmonella typhi.Conclusions:These results suggest that this plant contains relatively good antibacterial activity and it can be used as a source of antiseptic compounds for medicinal uses.
