Antibiotic-induced dysbiosis is a key factor that causes Clostridioides difficile infection(CDI).The current first-line drugs for initial CDI are antibiotics,and finding an alternate therapy to prevent recurrent CDI i...Antibiotic-induced dysbiosis is a key factor that causes Clostridioides difficile infection(CDI).The current first-line drugs for initial CDI are antibiotics,and finding an alternate therapy to prevent recurrent CDI is essential.In this study,the anti-C.difficile activities of two isolated probiotic strains(Pediococcus pentosaceus CD5 and Lactobacillus salivarius CD4)and grapefruit seed extract(GSE)were investigated in vitro and in vivo.Specifically,GSE inhibited the growth of C.difficile at a minimal inhibitory concentration(MIC)of 40 ppm.Similarly,live cells of the two probiotics,as well as their cell-free culture supernatants,with or without pH adjustment,substantially suppressed C.difficile growth.In a CDI mouse model,C.difficile colonization in the cecum and colon inflammation were significantly reduced by GSE,followed by the probiotic treatments.A metagenomic analysis of cecal feces revealed that GSE and probiotic treatments modulated gut microbial compositions and enhanced the relative abundances of butyrate-producing bacteria.Therefore,GSE and the novel probiotics can potentially be administered as alternative therapies to alleviate CDI by modifying the gut microbiota.展开更多
基金supported by various funding sources,including the National Natural Science Foundation of China(No.32302101)the Natural Science Foundation of Hubei Province(2023AFB439)+3 种基金the TAMU New Faculty Startup Fundsthe BIFIDO grantthe BIFIDO-TAMU AgriLife Research grant(2400954)the Industrial Cluster Program(#PGW22003)funded by the Ministry of Trade,Industry&Energy(MOTIE,Republic of Korea).
文摘Antibiotic-induced dysbiosis is a key factor that causes Clostridioides difficile infection(CDI).The current first-line drugs for initial CDI are antibiotics,and finding an alternate therapy to prevent recurrent CDI is essential.In this study,the anti-C.difficile activities of two isolated probiotic strains(Pediococcus pentosaceus CD5 and Lactobacillus salivarius CD4)and grapefruit seed extract(GSE)were investigated in vitro and in vivo.Specifically,GSE inhibited the growth of C.difficile at a minimal inhibitory concentration(MIC)of 40 ppm.Similarly,live cells of the two probiotics,as well as their cell-free culture supernatants,with or without pH adjustment,substantially suppressed C.difficile growth.In a CDI mouse model,C.difficile colonization in the cecum and colon inflammation were significantly reduced by GSE,followed by the probiotic treatments.A metagenomic analysis of cecal feces revealed that GSE and probiotic treatments modulated gut microbial compositions and enhanced the relative abundances of butyrate-producing bacteria.Therefore,GSE and the novel probiotics can potentially be administered as alternative therapies to alleviate CDI by modifying the gut microbiota.
