Background:This research aimed to examine the impact of co-transplantation of ethyl stearate(PubChem CID:8122)and neural stem cells(NSCs)on endogenous NSCs in Parkinson's disease(PD)model rats,and also determine t...Background:This research aimed to examine the impact of co-transplantation of ethyl stearate(PubChem CID:8122)and neural stem cells(NSCs)on endogenous NSCs in Parkinson's disease(PD)model rats,and also determine the impact of ethyl stearate on quiescent neural stem cells(qNSCs)and explore its possible mechanism.Methods:The induction of PD rats was achieved through the injection of 6-hydroxydopamine(6-OHDA)into the right striatum(STR)and substantia nigra(SN).Transplant treatment was then followed for 6 weeks in the right STR.Apomorphine(APO)-induced rotation behavior and the pole climbing test were used to assess the effects of transplant treatment in each group.Western blotting(WB)and immunofluorescence staining were used to observe changes in endogenous NSCs.The effect of ethyl stearate on qNSCs was examined in vitro,with detection of associated indicators achieved through WB,immunofluorescence,flow cytometry and qRT-PCR.Results:Co-transplantation of NSCs with ethyl stearate significantly ameliorated motor deficits in PD rats and upregulated the expression of tyrosine hydroxylase(TH),Sox2,and Nestin.We established an in vitro system for NSC quiescence and activation.In qNSCs,ethyl stearate treatment increased Ki67 positivity and reduced the proportion of cells in the G0-G1 phase of the cell cycle,whereas withdrawal of ethyl stearate exerted the opposite effects.Ethyl stearate also downregulated EphB2 protein levels and upregulated p-AKT and CyclinD1 in qNSCs;these effects were reversed upon its removal.Conclusion:These findings imply that co-transplantation of NSCs and ethyl stearate may help ameliorate motor impairment in PD rats by activating endogenous NSCs and promoting their development into dopaminergic neurons.Ethyl stearate may regulate the entry and exit of NSCs from quiescence through the EphB2/AKT/CyclinD1 pathway.展开更多
BACKGROUND The combined anterior/posterior approach appears to be capable of reconstructing spinal stability,correcting thoracolumbar deformity,and promoting neural recovery in severe thoracolumbar fracture dislocatio...BACKGROUND The combined anterior/posterior approach appears to be capable of reconstructing spinal stability,correcting thoracolumbar deformity,and promoting neural recovery in severe thoracolumbar fracture dislocation.However,this type of operation requires changing the body position during the procedure,resulting in a lengthy operation time.As a universal surgical robot,TINAVI robot has achieved good surgical results in clinical surgery.But to our knowledge,no reports describing TINAVI robot-assisted single lateral position anteroposterior surgery for thoracolumbar fracture dislocation.CASE SUMMARY We describe a case of a 16-year-old female patient with severe thoracolumbar fracture and dislocation underwent surgery assisted by the TINAVI robot.A onestage combined anterior and posterior operation was performed on a severe thoracolumbar fracture dislocation using the TINAVI robot,and the operation was completed in right lateral position.CONCLUSION The TINAVI robot-assisted one-stage anterior and posterior surgery in right lateral position for severe thoracolumbar fracture and dislocation is both safe and effective.展开更多
Probes for in vivo imaging of hypochlorous acid(HOCl),one of the most important reactive oxygen species in innate immunity,are urgently needed to understand the pathogenesis of autoimmune and neuroinflammatory disorde...Probes for in vivo imaging of hypochlorous acid(HOCl),one of the most important reactive oxygen species in innate immunity,are urgently needed to understand the pathogenesis of autoimmune and neuroinflammatory disorders.As a strong oxidant,HOCl could bleach near-infrared sensors and inactivate luciferase readily,making in vivo imaging overwhelmingly challenging.Via fine-tuning of a selective HOCl sensing moiety,HOCl stable spacer,and bright chemiluminescent scaffold,we have developed HOCl-CL-510 as a highly selective and sensitive probe for HOCl detection both in vitro and in vivo.In particular,we achieved recurring real-time monitoring of HOCl in both acute and chronic inflammation models in living mice,providing a new chemical tool for dynamic monitoring of disease development with reduced usage of experimental animals.展开更多
基金The Medical Scientific Research Foundation of Guangdong Province,Grant/Award Number:B2020002National Natural Science Foundation of China,Grant/Award Number:82474266Science and Technology Planning Project of Guangdong Province,Grant/Award Number:2016A020226017。
文摘Background:This research aimed to examine the impact of co-transplantation of ethyl stearate(PubChem CID:8122)and neural stem cells(NSCs)on endogenous NSCs in Parkinson's disease(PD)model rats,and also determine the impact of ethyl stearate on quiescent neural stem cells(qNSCs)and explore its possible mechanism.Methods:The induction of PD rats was achieved through the injection of 6-hydroxydopamine(6-OHDA)into the right striatum(STR)and substantia nigra(SN).Transplant treatment was then followed for 6 weeks in the right STR.Apomorphine(APO)-induced rotation behavior and the pole climbing test were used to assess the effects of transplant treatment in each group.Western blotting(WB)and immunofluorescence staining were used to observe changes in endogenous NSCs.The effect of ethyl stearate on qNSCs was examined in vitro,with detection of associated indicators achieved through WB,immunofluorescence,flow cytometry and qRT-PCR.Results:Co-transplantation of NSCs with ethyl stearate significantly ameliorated motor deficits in PD rats and upregulated the expression of tyrosine hydroxylase(TH),Sox2,and Nestin.We established an in vitro system for NSC quiescence and activation.In qNSCs,ethyl stearate treatment increased Ki67 positivity and reduced the proportion of cells in the G0-G1 phase of the cell cycle,whereas withdrawal of ethyl stearate exerted the opposite effects.Ethyl stearate also downregulated EphB2 protein levels and upregulated p-AKT and CyclinD1 in qNSCs;these effects were reversed upon its removal.Conclusion:These findings imply that co-transplantation of NSCs and ethyl stearate may help ameliorate motor impairment in PD rats by activating endogenous NSCs and promoting their development into dopaminergic neurons.Ethyl stearate may regulate the entry and exit of NSCs from quiescence through the EphB2/AKT/CyclinD1 pathway.
文摘BACKGROUND The combined anterior/posterior approach appears to be capable of reconstructing spinal stability,correcting thoracolumbar deformity,and promoting neural recovery in severe thoracolumbar fracture dislocation.However,this type of operation requires changing the body position during the procedure,resulting in a lengthy operation time.As a universal surgical robot,TINAVI robot has achieved good surgical results in clinical surgery.But to our knowledge,no reports describing TINAVI robot-assisted single lateral position anteroposterior surgery for thoracolumbar fracture dislocation.CASE SUMMARY We describe a case of a 16-year-old female patient with severe thoracolumbar fracture and dislocation underwent surgery assisted by the TINAVI robot.A onestage combined anterior and posterior operation was performed on a severe thoracolumbar fracture dislocation using the TINAVI robot,and the operation was completed in right lateral position.CONCLUSION The TINAVI robot-assisted one-stage anterior and posterior surgery in right lateral position for severe thoracolumbar fracture and dislocation is both safe and effective.
基金supported by The University of Hong Kong,Morningside Foundation,Hong Kong Research Grants Council Area of Excellence Scheme(grant no.AoE/P-705/16 to D.Y.)National Natural Science Foundation of China(grant no.21961142011 to D.Y.)the Israel Science Foundation-China Joint Funding Program.
文摘Probes for in vivo imaging of hypochlorous acid(HOCl),one of the most important reactive oxygen species in innate immunity,are urgently needed to understand the pathogenesis of autoimmune and neuroinflammatory disorders.As a strong oxidant,HOCl could bleach near-infrared sensors and inactivate luciferase readily,making in vivo imaging overwhelmingly challenging.Via fine-tuning of a selective HOCl sensing moiety,HOCl stable spacer,and bright chemiluminescent scaffold,we have developed HOCl-CL-510 as a highly selective and sensitive probe for HOCl detection both in vitro and in vivo.In particular,we achieved recurring real-time monitoring of HOCl in both acute and chronic inflammation models in living mice,providing a new chemical tool for dynamic monitoring of disease development with reduced usage of experimental animals.
