Brassinosteroids( BRs),a group of polyhydroxylated plant steroid hormones,have fundamental functions in many aspects of plant growth and development. The BRI1-EMS SUPPRESSOR1( BES1) transcription factor is a positive ...Brassinosteroids( BRs),a group of polyhydroxylated plant steroid hormones,have fundamental functions in many aspects of plant growth and development. The BRI1-EMS SUPPRESSOR1( BES1) transcription factor is a positive regulator involved in BRs signaling pathways. We studied the role of At BES1D( obtained from bes1-D Arabidopsis) in tomato( Solanum lycopersicum) seed germination. Overexpression of At BES1D in tomato inhibited seed germination compared with wild type Zhongshusihao( ZS4). The expression of abscisic acid( ABA) related genes was enhanced in At BES1D transgenic tomato seeds during germination.Furthermore,At BES1D transgenic tomato seeds were hypersensitive to ABA. Our findings suggest that the inhibitory effect of At BES1D transcription factor on tomato seed germination may be correlated with an enhanced ABA pathway.展开更多
Background Recombinant erythropoietin(rEPO)is commonly used in therapy but may be abused in sports to enhance endurance.In doping analysis,rEPO can be detected in human urine or blood samples at picogram(pg)levels bas...Background Recombinant erythropoietin(rEPO)is commonly used in therapy but may be abused in sports to enhance endurance.In doping analysis,rEPO can be detected in human urine or blood samples at picogram(pg)levels based on its slightly higher molecular weight(MW)than that of endogenous EPO using western blotting(WB).However,a type of variant erythropoietin(VAR-EPO)encoded by the EPO c.577del variant has a similar MW to rEPO,and these 2 molecules cannot be distinguished using conventional analytical methods.A fit-for-purpose method needs to be developed immediately.Methods In this study,we introduced a reverse–normal immunopurification technique for sample pretreatment to remove VAR-EPO from samples to eliminate its interference with rEPO detection.Firstly,a rabbit monoclonal antibody(mAb)that can specifically recognize trace amounts of VAR-EPO with high affinity was generated.Then,using this antibody to enrich VAR-EPO,we developed reverse–normal immunopurification coupled with WB on the purpose of analyzing rEPO in urine and serum samples.Next,the method was fully validated and evaluated using blank samples,spiked samples and rEPO excreted samples.Finally,the identification criteria of rEPO was established.Results A specific anti-VAR mAb with high affinity was developed.Using it,we developed the doping analytical method for rEPO.Our method effectively detects and removes VAR-EPO,enabling accurate rEPO detection.Conclusion A method has already been applied for rEPO confirmation in routine doping analyses.展开更多
Objective:This study aimed to explore the impact of aortic annulus angulation on outcomes in patients with bicuspid aortic valves(BAVs)undergoing transcatheter aortic valve replacement(TAVR)with new-generation valves....Objective:This study aimed to explore the impact of aortic annulus angulation on outcomes in patients with bicuspid aortic valves(BAVs)undergoing transcatheter aortic valve replacement(TAVR)with new-generation valves.Methods:A retrospective study was conducted among patients who underwent TAVR with new-generation self-expandable(SE)or balloon-expandable(BE)transcatheter heart valves(THVs)at West China Hospital between January 2018 and November 2022.Their CT scans,from which the annulus angulation was derived,and other clinical information were retrieved through the electronic patient record system.The mean annulus angulation of the whole cohort served as the cut-off value for further group assignment.The primary endpoint was device success at 30 d.Secondary endpoints included the need for>1 THV,vascular complications,permanent pacemaker implantation,paravalvular leak of at least mild severity,30-day and 6-month mortality,etc.Multivariable logistic regression analysis was performed to identify independent predictors of the clinical outcomes.Results:A total of 180 patients were included in the study,137 with SE THVs and 43 with BE THVs.The annulus angulation of all patients was 55.6°±10.0°,therefore,the patients were further stratified by 56°.Among the 43 BE THV recipients,the annulus angulation≥56°group was older,shorter,had less valve calcification,and larger sinotubular junction and maximal ascending aorta diameters(all P<0.05);however,the primary and secondary endpoints did not differ significantly between the annulus angulation<56°and≥56°groups.Among the 137 SE THV recipients,despite larger maximal ascending aorta diameters in annulus angulation≥56°group((43.4±4.3)mm vs.(41.3±4.0)mm;P=0.003),it did not influence device success at 30 d,the requirement for>1 THV,vascular complications,paravalvular leak of at least mild severity,and 6-month mortality(all P>0.05).However,the SE THV group with annulus angulation≥56°had a higher incidence of permanent pacemaker implantation(27.6%vs.8.9%,P=0.004).Annulus angulation≥57°was an independent predictor of permanent pacemaker implantation in patients with BAV receiving SE TAVR(odds ratio:3.28,95%confidence interval:1.28-8.40;P=0.01).Conclusion:Increased annulus angulation did not influence device success for both new-generation BE and SE THVs in patients with BAV.However,increased annulus angulation was associated with a higher risk of permanent pacemaker implantation in patients undergoing SE TAVR,while no such association was observed in those who underwent BE TAVR.展开更多
A series of Au Pd@C nanoalloy catalysts with tunable compositions were successfully prepared by a co-reduction method. The use of borane-tert-butylamine complex as reductant and oleylamine as both solvent and reductan...A series of Au Pd@C nanoalloy catalysts with tunable compositions were successfully prepared by a co-reduction method. The use of borane-tert-butylamine complex as reductant and oleylamine as both solvent and reductant was very effective for the preparation of the monodispersed nanoalloy. We evaluated the catalytic activity of these Au Pd@C nanoalloys for oxidative dehydrogenative coupling of aniline, which showed better catalytic activity than equal amounts of sole Au@C or Pd@C catalyst. The Au1Pd3@C catalyst exhibited the best performance, indicating that the conversion and selectivity were improved along with the increase of Pd composition. However, if the Pd composition was too high in the Au Pd alloy, Au1Pd7@C achieved only 81% conversion in this reaction.展开更多
To the Editor:Transcatheter aortic valve replacement(TAVR)has become an established treatment for patients with severe aortic stenosis(AS)at all levels of surgical risk.^([1,2])With the exploration of TAVR and technol...To the Editor:Transcatheter aortic valve replacement(TAVR)has become an established treatment for patients with severe aortic stenosis(AS)at all levels of surgical risk.^([1,2])With the exploration of TAVR and technological developments,TAVR is an alternative for patients with intermediate or low surgical risk.^([3])There are some studies regarding gender differences in TAVR,but there are few studies on it in Asia.^([4])However,the studies of outcomes based on gender differences in TVAR are still lacking,especially in China and other Asian countries.展开更多
To the Editor:Approximately 2%of people>65 years old have aortic stenosis(AS).[1]Without intervention,AS is a rapidly progressive valvular heart disease with a 2-year mortality of 50%.[1]Since the first transcathet...To the Editor:Approximately 2%of people>65 years old have aortic stenosis(AS).[1]Without intervention,AS is a rapidly progressive valvular heart disease with a 2-year mortality of 50%.[1]Since the first transcatheter aortic valve replacement(TAVR)was performed by Cribier et al[2]in 2002,TAVR has been suggested to be non-inferior in AS patients of various risk stratifications.Post-TAVR complications are essential in predicting patient survival and improving quality of life,especially with the procedure being performed in an increasing population of low-risk patients.[3]Among them,myocardial infarction(MI)is a rare but life-threatening complication usually caused by the obstruction of coronary ostia.[1]In comparison,peri-procedural myocardial injury,primarily characterized by the elevation of cardiac markers,is a relatively common complication of TAVR.Although recent studies have demonstrated promising results of cardiac markers in predicting cardiovascular adverse events,the impact of peri-procedural myocardial injury on short-term and long-term mortality has yet to be established.展开更多
Percutaneous coronary intervention(PCI)is an important treatment strategy for patients with coronary artery disease.However,bleeding after PCI significantly increases the mortality risk.The search for prognostic predi...Percutaneous coronary intervention(PCI)is an important treatment strategy for patients with coronary artery disease.However,bleeding after PCI significantly increases the mortality risk.The search for prognostic predictors and optimal antiplatelet therapy for patients with high bleeding risk(HBR)after PCI has been a much researched upon topic in current cardiovascular research.However,there is no widely accepted prognostic model or recommended antiplatelet therapy for patients with PCI-HBR.In this trial,based on prospective multi-center database building,we will analyze the adverse prognostic predictors for patients with PCI-HBR,observe the types of antiplatelet drugs and duration of dual antiplatelet therapy in PCI-HBR patients,and compare the safety and feasibility of different antiplatelet regimens and treatment courses.The prognostic analysis and an appropriate antiplatelet strategy for patients with PCI and high bleeding risk(PPP-PCI)trial will help analyze bleeding risk factors in PCI-HBR patients and explore the appropriate antiplatelet treatment options.This study is registered with ClinicalTrials.gov(NCT05369442).The Research Ethics Committee of West China Hospital authorized this study(2022 Review#269).The trial results will be published in peer-reviewed journals and at conferences.展开更多
基金Supported by National Undergraduate Training Program for Innovation and Entrepreneurship (2018A8205)。
文摘Brassinosteroids( BRs),a group of polyhydroxylated plant steroid hormones,have fundamental functions in many aspects of plant growth and development. The BRI1-EMS SUPPRESSOR1( BES1) transcription factor is a positive regulator involved in BRs signaling pathways. We studied the role of At BES1D( obtained from bes1-D Arabidopsis) in tomato( Solanum lycopersicum) seed germination. Overexpression of At BES1D in tomato inhibited seed germination compared with wild type Zhongshusihao( ZS4). The expression of abscisic acid( ABA) related genes was enhanced in At BES1D transgenic tomato seeds during germination.Furthermore,At BES1D transgenic tomato seeds were hypersensitive to ABA. Our findings suggest that the inhibitory effect of At BES1D transcription factor on tomato seed germination may be correlated with an enhanced ABA pathway.
基金WADA and Beijing Sport University for funding under grant numbers 22B06XZ and 2022YB011
文摘Background Recombinant erythropoietin(rEPO)is commonly used in therapy but may be abused in sports to enhance endurance.In doping analysis,rEPO can be detected in human urine or blood samples at picogram(pg)levels based on its slightly higher molecular weight(MW)than that of endogenous EPO using western blotting(WB).However,a type of variant erythropoietin(VAR-EPO)encoded by the EPO c.577del variant has a similar MW to rEPO,and these 2 molecules cannot be distinguished using conventional analytical methods.A fit-for-purpose method needs to be developed immediately.Methods In this study,we introduced a reverse–normal immunopurification technique for sample pretreatment to remove VAR-EPO from samples to eliminate its interference with rEPO detection.Firstly,a rabbit monoclonal antibody(mAb)that can specifically recognize trace amounts of VAR-EPO with high affinity was generated.Then,using this antibody to enrich VAR-EPO,we developed reverse–normal immunopurification coupled with WB on the purpose of analyzing rEPO in urine and serum samples.Next,the method was fully validated and evaluated using blank samples,spiked samples and rEPO excreted samples.Finally,the identification criteria of rEPO was established.Results A specific anti-VAR mAb with high affinity was developed.Using it,we developed the doping analytical method for rEPO.Our method effectively detects and removes VAR-EPO,enabling accurate rEPO detection.Conclusion A method has already been applied for rEPO confirmation in routine doping analyses.
基金supported by Key Research and Development Project of Science&Technology Department of Sichuan Province(2022ZDZX0020)“1·3·5 project for disciplines of excellence-Clinical Research Fund,West China Hospital,Sichuan University”(23HXFH009)Sichuan Science and Technology Program(2022ZDZX003).
文摘Objective:This study aimed to explore the impact of aortic annulus angulation on outcomes in patients with bicuspid aortic valves(BAVs)undergoing transcatheter aortic valve replacement(TAVR)with new-generation valves.Methods:A retrospective study was conducted among patients who underwent TAVR with new-generation self-expandable(SE)or balloon-expandable(BE)transcatheter heart valves(THVs)at West China Hospital between January 2018 and November 2022.Their CT scans,from which the annulus angulation was derived,and other clinical information were retrieved through the electronic patient record system.The mean annulus angulation of the whole cohort served as the cut-off value for further group assignment.The primary endpoint was device success at 30 d.Secondary endpoints included the need for>1 THV,vascular complications,permanent pacemaker implantation,paravalvular leak of at least mild severity,30-day and 6-month mortality,etc.Multivariable logistic regression analysis was performed to identify independent predictors of the clinical outcomes.Results:A total of 180 patients were included in the study,137 with SE THVs and 43 with BE THVs.The annulus angulation of all patients was 55.6°±10.0°,therefore,the patients were further stratified by 56°.Among the 43 BE THV recipients,the annulus angulation≥56°group was older,shorter,had less valve calcification,and larger sinotubular junction and maximal ascending aorta diameters(all P<0.05);however,the primary and secondary endpoints did not differ significantly between the annulus angulation<56°and≥56°groups.Among the 137 SE THV recipients,despite larger maximal ascending aorta diameters in annulus angulation≥56°group((43.4±4.3)mm vs.(41.3±4.0)mm;P=0.003),it did not influence device success at 30 d,the requirement for>1 THV,vascular complications,paravalvular leak of at least mild severity,and 6-month mortality(all P>0.05).However,the SE THV group with annulus angulation≥56°had a higher incidence of permanent pacemaker implantation(27.6%vs.8.9%,P=0.004).Annulus angulation≥57°was an independent predictor of permanent pacemaker implantation in patients with BAV receiving SE TAVR(odds ratio:3.28,95%confidence interval:1.28-8.40;P=0.01).Conclusion:Increased annulus angulation did not influence device success for both new-generation BE and SE THVs in patients with BAV.However,increased annulus angulation was associated with a higher risk of permanent pacemaker implantation in patients undergoing SE TAVR,while no such association was observed in those who underwent BE TAVR.
基金supported by the National Natural Science Foundation of China(21072001,21372006)the Ministry of Education,the Ministry of Human Resources and Social Security,the Education Department of Anhui Province+2 种基金the Anhui Province International Scientific and Technological Cooperation Projectthe Natural Science Foundation of Education Department of Anhui Province(KJ2014A013)the 211 Project of Anhui University
文摘A series of Au Pd@C nanoalloy catalysts with tunable compositions were successfully prepared by a co-reduction method. The use of borane-tert-butylamine complex as reductant and oleylamine as both solvent and reductant was very effective for the preparation of the monodispersed nanoalloy. We evaluated the catalytic activity of these Au Pd@C nanoalloys for oxidative dehydrogenative coupling of aniline, which showed better catalytic activity than equal amounts of sole Au@C or Pd@C catalyst. The Au1Pd3@C catalyst exhibited the best performance, indicating that the conversion and selectivity were improved along with the increase of Pd composition. However, if the Pd composition was too high in the Au Pd alloy, Au1Pd7@C achieved only 81% conversion in this reaction.
基金supported by grants from the West China Hospital“1·3·5”Discipline of Excellence Project-“Mechanisms of aortic stenosis and the clinical applications”,National Natural Science Foundation of China(No.82001899)the National Natural Science Foundation of China(No.81901825).
文摘To the Editor:Transcatheter aortic valve replacement(TAVR)has become an established treatment for patients with severe aortic stenosis(AS)at all levels of surgical risk.^([1,2])With the exploration of TAVR and technological developments,TAVR is an alternative for patients with intermediate or low surgical risk.^([3])There are some studies regarding gender differences in TAVR,but there are few studies on it in Asia.^([4])However,the studies of outcomes based on gender differences in TVAR are still lacking,especially in China and other Asian countries.
基金supported by the National Natural Science Foundation of China(No.82001899)West China Hospital"1·3·5"Discipline of Excellence Project—"Mechanisms of aortic stenosis and the clinical applications".
文摘To the Editor:Approximately 2%of people>65 years old have aortic stenosis(AS).[1]Without intervention,AS is a rapidly progressive valvular heart disease with a 2-year mortality of 50%.[1]Since the first transcatheter aortic valve replacement(TAVR)was performed by Cribier et al[2]in 2002,TAVR has been suggested to be non-inferior in AS patients of various risk stratifications.Post-TAVR complications are essential in predicting patient survival and improving quality of life,especially with the procedure being performed in an increasing population of low-risk patients.[3]Among them,myocardial infarction(MI)is a rare but life-threatening complication usually caused by the obstruction of coronary ostia.[1]In comparison,peri-procedural myocardial injury,primarily characterized by the elevation of cardiac markers,is a relatively common complication of TAVR.Although recent studies have demonstrated promising results of cardiac markers in predicting cardiovascular adverse events,the impact of peri-procedural myocardial injury on short-term and long-term mortality has yet to be established.
基金supported by the 1-3-5 project for disciplines of excellence—Clinical Research Incubation Project,West China Hospital,Sichuan University(2019HXFH059 and 2021HXFH021)the Natural Science Foundation of China(82100282).
文摘Percutaneous coronary intervention(PCI)is an important treatment strategy for patients with coronary artery disease.However,bleeding after PCI significantly increases the mortality risk.The search for prognostic predictors and optimal antiplatelet therapy for patients with high bleeding risk(HBR)after PCI has been a much researched upon topic in current cardiovascular research.However,there is no widely accepted prognostic model or recommended antiplatelet therapy for patients with PCI-HBR.In this trial,based on prospective multi-center database building,we will analyze the adverse prognostic predictors for patients with PCI-HBR,observe the types of antiplatelet drugs and duration of dual antiplatelet therapy in PCI-HBR patients,and compare the safety and feasibility of different antiplatelet regimens and treatment courses.The prognostic analysis and an appropriate antiplatelet strategy for patients with PCI and high bleeding risk(PPP-PCI)trial will help analyze bleeding risk factors in PCI-HBR patients and explore the appropriate antiplatelet treatment options.This study is registered with ClinicalTrials.gov(NCT05369442).The Research Ethics Committee of West China Hospital authorized this study(2022 Review#269).The trial results will be published in peer-reviewed journals and at conferences.
