Global climate change and urbanization have posed challenges to sustainable food production and resource management in agriculture.Vertical farming,in particular,allows for high-density cultivation on limited land but...Global climate change and urbanization have posed challenges to sustainable food production and resource management in agriculture.Vertical farming,in particular,allows for high-density cultivation on limited land but requires precise control of crop height to suit vertical farming systems.Tomato,a globally significant vegetable crop,urgently requires mutant varieties that suppress indeterminate growth for effective cultivation in vertical farming systems.In this study,we utilized the CRISPR-Cas9 system to develop a new tomato cultivar optimized for vertical farming by editing the Gibberellin 20-oxidase(SlGA20ox)genes,which are well known for their roles in the"Green Revolution".Additionally,we proposed a volumetric model to effectively identify mutants through non-destructive analysis of chlorophyll fluorescence.The proposed model achieved over 84%classification accuracy in distinguishing triple-determinate and slga20ox gene-edited plants,outperforming traditional machine learning methods and 1D-CNN approaches.Unlike previous studies that primarily relied on manual feature extraction from chlorophyll fluorescence data,this research introduced a deep learning frame-work capable of automating feature extraction in three dimensions while learning the temporal characteristics of chlorophyll fluorescence imaging data.The study demonstrated the potential to classify tomato plants custom-ized for vertical farming,leveraging advanced phenotypic analysis methods.Our approach explores new analytical methods for chlorophyll fluorescence imaging data within AI-based phenotyping and can be extended to other crops and traits,accelerating breeding programs and enhancing the efficiency of genetic resource management.展开更多
基金This research was funded by the National Research Foundation of Korea(NRF)grant from the Ministry of Science and ICT(MSIT),Republic of Korea(RS-2024-00407469 and RS-2025-00517964)partially funded by the BK21 FOUR program of Graduate School,Kyung Hee University,Republic of Korea(GS-5-JO-NON-20250783).
文摘Global climate change and urbanization have posed challenges to sustainable food production and resource management in agriculture.Vertical farming,in particular,allows for high-density cultivation on limited land but requires precise control of crop height to suit vertical farming systems.Tomato,a globally significant vegetable crop,urgently requires mutant varieties that suppress indeterminate growth for effective cultivation in vertical farming systems.In this study,we utilized the CRISPR-Cas9 system to develop a new tomato cultivar optimized for vertical farming by editing the Gibberellin 20-oxidase(SlGA20ox)genes,which are well known for their roles in the"Green Revolution".Additionally,we proposed a volumetric model to effectively identify mutants through non-destructive analysis of chlorophyll fluorescence.The proposed model achieved over 84%classification accuracy in distinguishing triple-determinate and slga20ox gene-edited plants,outperforming traditional machine learning methods and 1D-CNN approaches.Unlike previous studies that primarily relied on manual feature extraction from chlorophyll fluorescence data,this research introduced a deep learning frame-work capable of automating feature extraction in three dimensions while learning the temporal characteristics of chlorophyll fluorescence imaging data.The study demonstrated the potential to classify tomato plants custom-ized for vertical farming,leveraging advanced phenotypic analysis methods.Our approach explores new analytical methods for chlorophyll fluorescence imaging data within AI-based phenotyping and can be extended to other crops and traits,accelerating breeding programs and enhancing the efficiency of genetic resource management.
