The single-molecule detection tech-nique plays a pivotal role in elucidat-ing the fundamental mechanisms of various scientific processes at the molecular level,and holds essential im-portance in multiple fields includ...The single-molecule detection tech-nique plays a pivotal role in elucidat-ing the fundamental mechanisms of various scientific processes at the molecular level,and holds essential im-portance in multiple fields including physics,biology,and chemistry.Re-cently,single-molecule detection has garnered increasing attention owing to its practical utility in medical diagno-sis,primarily due to its exceptional sensitivity and the minimal sample volume required for analysis.However,the conventional single-molecule technique,represented by total internal reflection microscopy,faces challenges such as sophisticated operation procedures and limited detection throughput,thereby impeding its broader application.To address these limitations,we have demonstrated single-molecule detection using an integrated silicon photonic chip,of-fering a cost-effective and user-friendly alternative.By employing basic optics,we efficiently introduce the excitation source for single-molecule fluorescence by harnessing the strong evanescent field of high refractive-index waveguides.Subsequently,fluorescence signals are collected using basic optics comprising a water-immersion objective,relay optics,and a digi-tal camera.Our results highlight a low-cost,high-throughput single-molecule technique achieved through the integrated silicon photonic chip.This innovative approach is promised to facilitate the widespread adoption of single-molecule fluorescence in medical diagnosis.展开更多
Miniaturized spectrometers for Raman spectroscopy have the potential to open up a new chapter in sensing.Raman spectroscopy is essential for material characterization and biomedical diagnostics,however,its weak signal...Miniaturized spectrometers for Raman spectroscopy have the potential to open up a new chapter in sensing.Raman spectroscopy is essential for material characterization and biomedical diagnostics,however,its weak signal and the need for sub-nanometer resolution pose challenges.Conventional spectrometers,with footprints proportional to optical throughput and resolution,are difficult to integrate into compact devices such as wearables.Waveguide-based Fourier Transform Spectrometers(FTS)enable compact spectrometers,and multi-aperture designs can achieve high throughput for applications such as Raman spectroscopy;however,experimental research in this domain remains limited.In this work,we present a multi-aperture SiN waveguide-based FTS overcoming these limitations and enabling Raman spectroscopy of isopropyl alcohol,glucose,Paracetamol,and Ibuprofen with enhanced throughput.Our spectrometer chip,fabricated on a 200mm SiN wafer,with 160 edge-coupled waveguide apertures connected to an array of ultra-compact interferometers and a small footprint of just 1.6mm×3.2 mm,achieves a spectral range of 40 nm and a resolution of 0.5 nm.Experimental results demonstrate that the least absolute shrinkage and selection operator(LASSO)regression significantly enhances Raman spectrum reconstruction.Our work on waveguide-based spectrometry paves the way for integrating accurate and compact Raman sensors into consumer electronics and space exploration instruments.展开更多
基金supported by the National Key Research and Development Program(No.2022YFE0107400)the internal research funding from Photonic View Technology Technology Co.,Ltd.the GuangCi Deep Mind Project of Ruijin Hospital Shanghai Jiao Tong University School of Medicine.
文摘The single-molecule detection tech-nique plays a pivotal role in elucidat-ing the fundamental mechanisms of various scientific processes at the molecular level,and holds essential im-portance in multiple fields including physics,biology,and chemistry.Re-cently,single-molecule detection has garnered increasing attention owing to its practical utility in medical diagno-sis,primarily due to its exceptional sensitivity and the minimal sample volume required for analysis.However,the conventional single-molecule technique,represented by total internal reflection microscopy,faces challenges such as sophisticated operation procedures and limited detection throughput,thereby impeding its broader application.To address these limitations,we have demonstrated single-molecule detection using an integrated silicon photonic chip,of-fering a cost-effective and user-friendly alternative.By employing basic optics,we efficiently introduce the excitation source for single-molecule fluorescence by harnessing the strong evanescent field of high refractive-index waveguides.Subsequently,fluorescence signals are collected using basic optics comprising a water-immersion objective,relay optics,and a digi-tal camera.Our results highlight a low-cost,high-throughput single-molecule technique achieved through the integrated silicon photonic chip.This innovative approach is promised to facilitate the widespread adoption of single-molecule fluorescence in medical diagnosis.
基金supported by the National Key Research and Development Program of China(No.2024YFF1206300)Guangci Innovative Technology Program(KY2023810)Guangci Talent Program(RC20240018).
文摘Miniaturized spectrometers for Raman spectroscopy have the potential to open up a new chapter in sensing.Raman spectroscopy is essential for material characterization and biomedical diagnostics,however,its weak signal and the need for sub-nanometer resolution pose challenges.Conventional spectrometers,with footprints proportional to optical throughput and resolution,are difficult to integrate into compact devices such as wearables.Waveguide-based Fourier Transform Spectrometers(FTS)enable compact spectrometers,and multi-aperture designs can achieve high throughput for applications such as Raman spectroscopy;however,experimental research in this domain remains limited.In this work,we present a multi-aperture SiN waveguide-based FTS overcoming these limitations and enabling Raman spectroscopy of isopropyl alcohol,glucose,Paracetamol,and Ibuprofen with enhanced throughput.Our spectrometer chip,fabricated on a 200mm SiN wafer,with 160 edge-coupled waveguide apertures connected to an array of ultra-compact interferometers and a small footprint of just 1.6mm×3.2 mm,achieves a spectral range of 40 nm and a resolution of 0.5 nm.Experimental results demonstrate that the least absolute shrinkage and selection operator(LASSO)regression significantly enhances Raman spectrum reconstruction.Our work on waveguide-based spectrometry paves the way for integrating accurate and compact Raman sensors into consumer electronics and space exploration instruments.
