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香蕉组织培养过程中内生菌污染的控制(英文) 被引量:6
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作者 Soubir Titov Salil Kumar Bhowmik +1 位作者 Md.Sadrul Alam sarder nasir uddin 《生物工程学报》 CAS CSCD 北大核心 2007年第6期1042-1048,共7页
研究了体外培养一种孟加拉传统香蕉(Musa spp.Cv.Kanthali)的茎尖组织。茎尖的原始细胞表面经无菌处理(0.1%HgCl2处理12min),接种6~15d后外植体地下茎部分仍有微生物污染(大部分是细菌),杀死了85%的外植体。为确定无污染... 研究了体外培养一种孟加拉传统香蕉(Musa spp.Cv.Kanthali)的茎尖组织。茎尖的原始细胞表面经无菌处理(0.1%HgCl2处理12min),接种6~15d后外植体地下茎部分仍有微生物污染(大部分是细菌),杀死了85%的外植体。为确定无污染培养基,将等量外植体分别浸泡在舍400mg/L氨苄青霉素和200mg/L庆大霉素(两种光谱抗生素)的培养基中1h。结果表明,经抗生素处理的外植体完全没有污染,但培养3周后不能再生。进行二次继代培养后,其中一部分外植体吸收了培养基并胀大,颜色由苍白转变成浅绿或深绿。三次继代培养后数天,不再观察到外植体的生长,所有经抗生素处理过的外植体都开始死亡。在未经抗生素处理的活外植体中,单个茎发育的最佳培养基是:Ms+4.0mg/LBA+0.5mg/L KT+15%CW,平均生长时间为18~21d,但再生率很低,只有30%。茎细胞增殖的最佳培养基是:MS+4.0mg/L BA+2.0mg/L IAA+15%CW,每个茎平均只萌发3~4个芽。最后,在添加0.5mg/L IBA的一半浓度的MS培养基中,体外培养茎最大生根率达到90%。 展开更多
关键词 香蕉 Kanthali 体外 茎尖 抗生素
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A multi-purpose National Forest Inventory in Bangladesh:design,operationalisation and key results
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作者 Matieu Henry Zaheer Iqbal +47 位作者 Kristofer Johnson Mariam Akhter Liam Costello Charles Scott Rashed Jalal Md.Akhter Hossain Nikhil Chakma Olaf Kuegler Hossain Mahmood Rajib Mahamud Mohammad Raqibul Hasan Siddique Khaled Misbahuzzaman Mohammad Main uddin Mohammed Al Amin Farid uddin Ahmed Gael Sola Md.Baktiar Siddiqui Luca Birigazzi Mahmudur Rahman Ilias Animon Saimunnahar Ritu Laskar Muqsudur Rahman Aminul Islam Heather Hayden Frida Sidik Mondal Falgoonee Kumar Rakibul Hassan Mukul Hossain Nishad Ariful Hoque Belal Asif Reza Anik Abdul Khaleque Md.Shaheduzzaman Syed Shahadat Hossain Tariq Aziz Md.Tauhidor Rahaman Ruhul Mohaiman Patrick Meyer Purnata Chakma A.Z.M.Manzoor Rashid Sourav Das Shrabanti Hira Mohammed Jashimuddin Mohammad Mahfuzur Rahman Karl Wurster sarder nasir uddin Abul Kalam Azad S.M.Zahirul Islam Laurent Saint-André 《Forest Ecosystems》 SCIE CSCD 2021年第1期151-172,共22页
Background:National forest inventory and forest monitoring systems are more important than ever considering continued global degradation of trees and forests.These systems are especially important in a country like Ba... Background:National forest inventory and forest monitoring systems are more important than ever considering continued global degradation of trees and forests.These systems are especially important in a country like Bangladesh,which is characterised by a large population density,climate change vulnerability and dependence on natural resources.With the aim of supporting the Government’s actions towards sustainable forest management through reliable information,the Bangladesh Forest Inventory(BFI)was designed and implemented through three components:biophysical inventory,socio-economic survey and remote sensing-based land cover mapping.This article documents the approach undertaken by the Forest Department under the Ministry of Environment,Forests and Climate Change to establish the BFI as a multipurpose,efficient,accurate and replicable national forest assessment.The design,operationalization and some key results of the process are presented.Methods:The BFI takes advantage of the latest and most well-accepted technological and methodological approaches.Importantly,it was designed through a collaborative process which drew from the experience and knowledge of multiple national and international entities.Overall,1781 field plots were visited,6400 households were surveyed,and a national land cover map for the year 2015 was produced.Innovative technological enhancements include a semi-automated segmentation approach for developing the wall-to-wall land cover map,an object-based national land characterisation system,consistent estimates between sample-based and mapped land cover areas,use of mobile apps for tree species identification and data collection,and use of differential global positioning system for referencing plot centres.Results:Seven criteria,and multiple associated indicators,were developed for monitoring progress towards sustainable forest management goals,informing management decisions,and national and international reporting needs.A wide range of biophysical and socioeconomic data were collected,and in some cases integrated,for estimating the indicators.Conclusions:The BFI is a new information source tool for helping guide Bangladesh towards a sustainable future.Reliable information on the status of tree and forest resources,as well as land use,empowers evidence-based decision making across multiple stakeholders and at different levels for protecting natural resources.The integrated socioeconomic data collected provides information about the interactions between people and their tree and forest resources,and the valuation of ecosystem services.The BFI is designed to be a permanent assessment of these resources,and future data collection will enable monitoring of trends against the current baseline.However,additional institutional support as well as continuation of collaboration among national partners is crucial for sustaining the BFI process in future. 展开更多
关键词 South-Asia Forest monitoring Innovation Remote sensing SOCIO-ECONOMIC PARTNERSHIP Ecosystem services Carbon sequestration
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