Abdominal wall patches are clinically essential for treating abdominal defects or hernias,with mechanical strength representing a critical requirement.Therefore,rational scaffold design and fabrication methods are cru...Abdominal wall patches are clinically essential for treating abdominal defects or hernias,with mechanical strength representing a critical requirement.Therefore,rational scaffold design and fabrication methods are crucial for achieving optimal performance.This study introduces an innovative approach to the design of scaffolds for abdominal wall repair,using topology optimization and melt electrowriting(MEW).Through topology optimization,we provided a systematic,data-driven basis for scaffold design.We further refined the scaffold structure to enhance print efficiency and continuity,and successfully implemented MEW as fabrication technology,marking its first application in abdominal repair.Mechanical testing revealed that the topology-optimized scaffold achieved abdominal tensile strength of 1.85±0.02 N/cm,39%superior to conventional designs.Subsequent biological assessments-including fibroblast proliferation and alignment analyses-showed that collagen coating significantly enhanced cell attachment and proliferation,especially in multi-layer(300 layers)scaffolds,maintaining diameters of 11.34±0.67μm throughout the depth.Finally,ex vivo porcine abdominal wall tests confirmed clinical mechanical suitability.This work offers a promising direction for future advancements in tissue engineering,particularly in optimizing scaffold structures for biological and mechanical performance.展开更多
基金funding support from the China Scholarship Council(Grant No.202206890038).
文摘Abdominal wall patches are clinically essential for treating abdominal defects or hernias,with mechanical strength representing a critical requirement.Therefore,rational scaffold design and fabrication methods are crucial for achieving optimal performance.This study introduces an innovative approach to the design of scaffolds for abdominal wall repair,using topology optimization and melt electrowriting(MEW).Through topology optimization,we provided a systematic,data-driven basis for scaffold design.We further refined the scaffold structure to enhance print efficiency and continuity,and successfully implemented MEW as fabrication technology,marking its first application in abdominal repair.Mechanical testing revealed that the topology-optimized scaffold achieved abdominal tensile strength of 1.85±0.02 N/cm,39%superior to conventional designs.Subsequent biological assessments-including fibroblast proliferation and alignment analyses-showed that collagen coating significantly enhanced cell attachment and proliferation,especially in multi-layer(300 layers)scaffolds,maintaining diameters of 11.34±0.67μm throughout the depth.Finally,ex vivo porcine abdominal wall tests confirmed clinical mechanical suitability.This work offers a promising direction for future advancements in tissue engineering,particularly in optimizing scaffold structures for biological and mechanical performance.
