BACKGROUND Vasculitis,a systemic disorder with inflammation of blood vessel walls,can develop broad spectrum of signs and symptoms according to involvement of various organs,and therefore,early diagnosis of vasculitis...BACKGROUND Vasculitis,a systemic disorder with inflammation of blood vessel walls,can develop broad spectrum of signs and symptoms according to involvement of various organs,and therefore,early diagnosis of vasculitis is challenging.We herein describe a patient who developed a special case of systemic vasculitis with mononeuropathy multiplex,rectal perforation and antiphospholipid syndrome(APS)presented with pulmonary embolism.CASE SUMMARY A 61-year-old woman visited hospital with complaints of myalgia and occasional fever.She was initially diagnosed as proctitis and treated with antibiotics,however,there was no improvement.In addition,she also complained right foot drop with hypesthesia,and left 2^(nd) and 3^(rd) finger tingling sensation.She underwent nerve conduction study for evaluation,and it revealed sensorimotor polyneuropathy in the left arm and bilateral legs.Subsequent sural nerve biopsy strongly suggested vasculitic neuropathy.Based on nerve biopsy and clinical manifestation,she was diagnosed with vasculitis and treated with immunosuppressive therapy.During treatment,sudden rectal perforation and pulmonary thromboembolism occurred,and further laboratory study suggested probable concomitant APS.Emergency Hartmann operation was performed for rectal perforation,and anti-coagulation therapy was started for APS.After few cycles of immunosuppressive therapy,tingling sensation and weakness in her hand and foot had been partially recovered and vasculitis was considered to be stationary.CONCLUSION Vasculitis can be presented with a variety of signs and symptoms,therefore,clinicians should always consider the possibility of diagnosis.展开更多
To overcome Type 1 diabetes mellitus(T1DM),which can cause hyperglycemia due to diminished insulin secretion ofβ-cell function,islet transplantation has been developed with various strategies including pseudo-islet.H...To overcome Type 1 diabetes mellitus(T1DM),which can cause hyperglycemia due to diminished insulin secretion ofβ-cell function,islet transplantation has been developed with various strategies including pseudo-islet.However,conventional pseudo-islet formation techniques combining with other cells depend on natural cellular aggregation,which requires at least 5 days to form and even show segregation of distinct cell types,leading to diminished cell viability and function.Herein,we applied a subaqueous free-standing 3D cell culture(FS)device,which can reduce the spheroid formation time by trapped cell in nodes of acoustic standing wave.Briefly,Culturing with adipose-derived stem cells(ADSCs)to form heterotypic pseudo-islet(Hislet)in FS device dramatically reduced formation time less than one day.Hislet demonstrated enhancement of cell viability than conventional pseudo-islet formation method.Additionally,ADSCs combined Hislet proved strong secretion of various paracrine factors.Also results showed significantly increased angiogenesis effect and immunomodulation effect for various type of immune cells in Hislet compared to islet,which can enhance transplantation survival.Furthermore,Hislet validated glucose-regulating capacity and enhanced angiogenesis effect in vivo T1DM model.Throughout this study,we propose a novel strategy for forming Hislet that can overcome the limitations of conventional Islet and pseudo-islet for T1DM.展开更多
文摘BACKGROUND Vasculitis,a systemic disorder with inflammation of blood vessel walls,can develop broad spectrum of signs and symptoms according to involvement of various organs,and therefore,early diagnosis of vasculitis is challenging.We herein describe a patient who developed a special case of systemic vasculitis with mononeuropathy multiplex,rectal perforation and antiphospholipid syndrome(APS)presented with pulmonary embolism.CASE SUMMARY A 61-year-old woman visited hospital with complaints of myalgia and occasional fever.She was initially diagnosed as proctitis and treated with antibiotics,however,there was no improvement.In addition,she also complained right foot drop with hypesthesia,and left 2^(nd) and 3^(rd) finger tingling sensation.She underwent nerve conduction study for evaluation,and it revealed sensorimotor polyneuropathy in the left arm and bilateral legs.Subsequent sural nerve biopsy strongly suggested vasculitic neuropathy.Based on nerve biopsy and clinical manifestation,she was diagnosed with vasculitis and treated with immunosuppressive therapy.During treatment,sudden rectal perforation and pulmonary thromboembolism occurred,and further laboratory study suggested probable concomitant APS.Emergency Hartmann operation was performed for rectal perforation,and anti-coagulation therapy was started for APS.After few cycles of immunosuppressive therapy,tingling sensation and weakness in her hand and foot had been partially recovered and vasculitis was considered to be stationary.CONCLUSION Vasculitis can be presented with a variety of signs and symptoms,therefore,clinicians should always consider the possibility of diagnosis.
基金supported by the National Research Foundation(NRF)grants funded by the Ministry of Science and ICT(Grant No.RS-2023-00272815 to J-H.J.,RS-2024-00411474 to H-J.P)the Korean Fund for Regenerative Medicine(KFRM)grant funded by the Korea government(the Ministry of Science and ICT,the Ministry of Health&Welfare)(No.21A0102L1-13 to S.H.B.and KFRM24A0105L1 to D.Y.L.,Republic of Koea)+1 种基金supported by the Alchemist Project of the Korea Evaluation Institute of Industrial Technology(KEIT 20018560,NTIS 2410005252 to S.H.B.,H-J.P,and K.D.P)the Ministry of Trade,Industry&Energy,Republic of Korea.
文摘To overcome Type 1 diabetes mellitus(T1DM),which can cause hyperglycemia due to diminished insulin secretion ofβ-cell function,islet transplantation has been developed with various strategies including pseudo-islet.However,conventional pseudo-islet formation techniques combining with other cells depend on natural cellular aggregation,which requires at least 5 days to form and even show segregation of distinct cell types,leading to diminished cell viability and function.Herein,we applied a subaqueous free-standing 3D cell culture(FS)device,which can reduce the spheroid formation time by trapped cell in nodes of acoustic standing wave.Briefly,Culturing with adipose-derived stem cells(ADSCs)to form heterotypic pseudo-islet(Hislet)in FS device dramatically reduced formation time less than one day.Hislet demonstrated enhancement of cell viability than conventional pseudo-islet formation method.Additionally,ADSCs combined Hislet proved strong secretion of various paracrine factors.Also results showed significantly increased angiogenesis effect and immunomodulation effect for various type of immune cells in Hislet compared to islet,which can enhance transplantation survival.Furthermore,Hislet validated glucose-regulating capacity and enhanced angiogenesis effect in vivo T1DM model.Throughout this study,we propose a novel strategy for forming Hislet that can overcome the limitations of conventional Islet and pseudo-islet for T1DM.
