AIM To determine influence of neoadjuvant-chemotherapy(NAC)over tumor-infiltrating-lymphocytes(TIL)intriple-negative-breast-cancer(TNBC).METHODS TILs were evaluated in 98 TNBC cases who came to Instituto Nacional de E...AIM To determine influence of neoadjuvant-chemotherapy(NAC)over tumor-infiltrating-lymphocytes(TIL)intriple-negative-breast-cancer(TNBC).METHODS TILs were evaluated in 98 TNBC cases who came to Instituto Nacional de Enfermedades Neoplasicas from 2005 to 2010.Immunohistochemistry staining for CD3,CD4,CD8 and FOXP3 was performed in tissue microarrays(TMA)sections.Evaluation of H/E in full-face and immunohistochemistry in TMA sections was performed in pre and post-NAC samples.STATA software was used and P value<0.05 was considered statistically significant.RESULTS Higher TIL evaluated in full-face sections from pre-NAC tumors was associated to pathologic-complete-response(pCR)(P=0.0251)and outcome(P=0.0334).TIL evaluated in TMA sections showed low level of agreement with full-face sections(ICC=0.017-0.20)and was not associated to pCR or outcome.TIL in post-NAC samples were not associated to response or outcome.PostNAC lesions with pC R had similar TIL levels than those without pCR(P=0.6331).NAC produced a TIL decrease in full-face sections(P<0.0001).Percentage of TIL subpopulations was correlated with their absolute counts.Higher counts of CD3,CD4,CD8 and FOXP3 in pre-NAC samples had longer disease-free-survival(DFS).Higher counts of CD3 in pre-NAC samples had longer overallsurvival.Higher ratio of CD8/CD4 counts in pre-NAC was associated with pCR.Higher ratio of CD4/FOXP3 counts in pre-NAC was associated with longer DFS.Higher counts of CD4 in post-NAC samples were associated with pCR.CONCLUSION TIL in pre-NAC full-face sections in TNBC are correlated to longer survival.TIL in full-face differ from TMA sections,absolute count and percentage analysis of TIL subpopulation closely related.展开更多
Aim:Upper alveolar ridge and hard palate squamous cancer is an infrequent malignancy.We evaluated factors associated with neck involvement and with p16-staining.Methods:Head and neck squamous-cell carcinoma(SCC)patien...Aim:Upper alveolar ridge and hard palate squamous cancer is an infrequent malignancy.We evaluated factors associated with neck involvement and with p16-staining.Methods:Head and neck squamous-cell carcinoma(SCC)patients who went to Head and Neck Department between 1997 and 2011 were screened,and 73 resected upper alveolar ridge and 5 hard palate SCC were selected.Tumors with available tissue were stained with p16 immunohistochemistry.Results:Median age was 64.4 years,55.1%were female,and 73.1%were in clinical stage IV.Neck dissections were performed in 24 and pathologically confirmed node metastases were found in 19(24.3%).Cervical recurrence was found in 18 patients(23.1%)and was associated with histological grade(P=0.037).Three(7.3%)of 41 lesions were positive for p16 and tended to be younger(P=0.067).Lymphovascular invasion was associated with shorter disease-free survival(DFS)(P=0.026)and overall survival(OS)(P=0.021).Larger cT(P=0.019),perineural invasion(P=0.039)and neck dissection(P=0.010)were associated with shorter OS.Neck node involvement tended to have shorter DFS(31%vs.48.7%,P=0.278)and OS(25.1%vs.48.5%,P=0.340),and neck recurrence tended to have shorter OS(9.3%vs.52.3%,P=0.064).Conclusion:Neck involvement and recurrence are frequent in this location.P16-positive cases were present in 7.3%and tended to be associated with younger age.展开更多
文摘AIM To determine influence of neoadjuvant-chemotherapy(NAC)over tumor-infiltrating-lymphocytes(TIL)intriple-negative-breast-cancer(TNBC).METHODS TILs were evaluated in 98 TNBC cases who came to Instituto Nacional de Enfermedades Neoplasicas from 2005 to 2010.Immunohistochemistry staining for CD3,CD4,CD8 and FOXP3 was performed in tissue microarrays(TMA)sections.Evaluation of H/E in full-face and immunohistochemistry in TMA sections was performed in pre and post-NAC samples.STATA software was used and P value<0.05 was considered statistically significant.RESULTS Higher TIL evaluated in full-face sections from pre-NAC tumors was associated to pathologic-complete-response(pCR)(P=0.0251)and outcome(P=0.0334).TIL evaluated in TMA sections showed low level of agreement with full-face sections(ICC=0.017-0.20)and was not associated to pCR or outcome.TIL in post-NAC samples were not associated to response or outcome.PostNAC lesions with pC R had similar TIL levels than those without pCR(P=0.6331).NAC produced a TIL decrease in full-face sections(P<0.0001).Percentage of TIL subpopulations was correlated with their absolute counts.Higher counts of CD3,CD4,CD8 and FOXP3 in pre-NAC samples had longer disease-free-survival(DFS).Higher counts of CD3 in pre-NAC samples had longer overallsurvival.Higher ratio of CD8/CD4 counts in pre-NAC was associated with pCR.Higher ratio of CD4/FOXP3 counts in pre-NAC was associated with longer DFS.Higher counts of CD4 in post-NAC samples were associated with pCR.CONCLUSION TIL in pre-NAC full-face sections in TNBC are correlated to longer survival.TIL in full-face differ from TMA sections,absolute count and percentage analysis of TIL subpopulation closely related.
文摘Aim:Upper alveolar ridge and hard palate squamous cancer is an infrequent malignancy.We evaluated factors associated with neck involvement and with p16-staining.Methods:Head and neck squamous-cell carcinoma(SCC)patients who went to Head and Neck Department between 1997 and 2011 were screened,and 73 resected upper alveolar ridge and 5 hard palate SCC were selected.Tumors with available tissue were stained with p16 immunohistochemistry.Results:Median age was 64.4 years,55.1%were female,and 73.1%were in clinical stage IV.Neck dissections were performed in 24 and pathologically confirmed node metastases were found in 19(24.3%).Cervical recurrence was found in 18 patients(23.1%)and was associated with histological grade(P=0.037).Three(7.3%)of 41 lesions were positive for p16 and tended to be younger(P=0.067).Lymphovascular invasion was associated with shorter disease-free survival(DFS)(P=0.026)and overall survival(OS)(P=0.021).Larger cT(P=0.019),perineural invasion(P=0.039)and neck dissection(P=0.010)were associated with shorter OS.Neck node involvement tended to have shorter DFS(31%vs.48.7%,P=0.278)and OS(25.1%vs.48.5%,P=0.340),and neck recurrence tended to have shorter OS(9.3%vs.52.3%,P=0.064).Conclusion:Neck involvement and recurrence are frequent in this location.P16-positive cases were present in 7.3%and tended to be associated with younger age.
