This study extensively investigated to find out the in-vivo acute toxicity and lethal concentration(LC50)of biosynthesized Ag NPs in Labeo rohita(L.rohita).The haematological studies and antioxidative responses were c...This study extensively investigated to find out the in-vivo acute toxicity and lethal concentration(LC50)of biosynthesized Ag NPs in Labeo rohita(L.rohita).The haematological studies and antioxidative responses were considered in three tissues such as gill,liver and muscle of L.rohita.The results of this study showed that increasing the dose of Ag NPs led to bioaccumulation in the tissues.The haematological analysis showed considerable alterations in the Ag NPs-treated fish.The impact of histological changes induced by Ag NPs were confirmed by the damages in the tissues,primary lamella,blood vessels and formation of vacuolation in liver and muscle when compared with the control L.rohita.展开更多
Chitosan is a natural polymer that can be modified into chemically different types.In order to imply biomedical applications,the chitosan dissolved in aqueous medium.Therefore,in the present study,functionally substit...Chitosan is a natural polymer that can be modified into chemically different types.In order to imply biomedical applications,the chitosan dissolved in aqueous medium.Therefore,in the present study,functionally substituted chemical groups such as O-carboxymethyl chitosan(CMC),N-succinyl chitosan(NSC),quaternary ammonium chitosan(QAC)and tripoly phosphate chitosan(TPC)were synthesized.The peptide derived from Clarias batrachus was used as a drug that is successfully entrapped with NSC nanoparticles and were characterized by FTIR,AFM,SEM,Zeta sizer,fluorescent imaging and SDS-PAGE.In addition,antimicrobial activity was examined.The results of NSC obtained particle size(~64 nm)with highest solubility and 80%of antimicrobial activity against multidrug resistant pathogen Klebsiella pneumonia.Finally,the release profile indicates that the NSC nanoparticles deliver the peptide with 75%sustainable release and good availability up to 96 h.The current research was the first to report the characterization of peptide with NSC nanoparticles by SDS-PAGE,in which the antimicrobial activity was enhanced up to 80%,the particle sizes were obtained~64 nm from SEM and AFM,and NSC-peptide exhibited 75%of sustainable release and good availability up to 96 h.展开更多
文摘This study extensively investigated to find out the in-vivo acute toxicity and lethal concentration(LC50)of biosynthesized Ag NPs in Labeo rohita(L.rohita).The haematological studies and antioxidative responses were considered in three tissues such as gill,liver and muscle of L.rohita.The results of this study showed that increasing the dose of Ag NPs led to bioaccumulation in the tissues.The haematological analysis showed considerable alterations in the Ag NPs-treated fish.The impact of histological changes induced by Ag NPs were confirmed by the damages in the tissues,primary lamella,blood vessels and formation of vacuolation in liver and muscle when compared with the control L.rohita.
文摘Chitosan is a natural polymer that can be modified into chemically different types.In order to imply biomedical applications,the chitosan dissolved in aqueous medium.Therefore,in the present study,functionally substituted chemical groups such as O-carboxymethyl chitosan(CMC),N-succinyl chitosan(NSC),quaternary ammonium chitosan(QAC)and tripoly phosphate chitosan(TPC)were synthesized.The peptide derived from Clarias batrachus was used as a drug that is successfully entrapped with NSC nanoparticles and were characterized by FTIR,AFM,SEM,Zeta sizer,fluorescent imaging and SDS-PAGE.In addition,antimicrobial activity was examined.The results of NSC obtained particle size(~64 nm)with highest solubility and 80%of antimicrobial activity against multidrug resistant pathogen Klebsiella pneumonia.Finally,the release profile indicates that the NSC nanoparticles deliver the peptide with 75%sustainable release and good availability up to 96 h.The current research was the first to report the characterization of peptide with NSC nanoparticles by SDS-PAGE,in which the antimicrobial activity was enhanced up to 80%,the particle sizes were obtained~64 nm from SEM and AFM,and NSC-peptide exhibited 75%of sustainable release and good availability up to 96 h.
