Hunan is located at the evolutionary transitional zone edge of the Yunnan-Guizhou plateau,where tea germplasms are abundant.At present,although morphological,enzymological and molecular markers have been used to study...Hunan is located at the evolutionary transitional zone edge of the Yunnan-Guizhou plateau,where tea germplasms are abundant.At present,although morphological,enzymological and molecular markers have been used to study the genetic relationship of these tea germplasms.However,due to the complexity of the genetic background of tea germplasm resources,the evolution history and taxonomy of Hunan tea germplasm is still unclear.In this study,four Hunan tea landraces from the evolutionary transitional zone were collected,including nine'Chengbu Dongcha'(CBDC),eight'Jianghua Kucha'(JHKC),11'Rucheng Baimao'(RCBM)and eight'Anhua Yuntai'(AHYT).We used whole-genome re-sequencing to identify genetic variation,phylogenetic placement,and population structure among the four tea landraces and then scanned the genome for regions related to population potential selective.Across the samples,we identified an average of 1.38 million(M)SNPs and 6.82×10^(3)INDELs.Based on the high-quality SNPs,we performed neighbor-joining phylogenetic tree,population structure,and principal component analysis,all of which supported that the 36 tea germplasms could been divided into two groups,CBDC and AHYT were clustered into a group,and RCBM and JHKC were clustered into another group.With CBDC,JHKC,and RCBM as control,407,380,and 147 selected genes were identified in AHYT,respectively,were in the top 5%of F_(ST)and Log_(2)^(Ratioθπ)scores.The identified genes are mainly involved in stress tolerance,biosynthesis of important secondary metabolites,adaptability,and yield.These results will be helpful in the classification of tea germplasms and investigating selection–related genes.展开更多
Tea is considered to be a well-known and widely consumed beverage and Hunan province is rich in tea plant germplasm.In order to better conserve and utilize Hunan tea plant resources,110 tea accessions from seven geogr...Tea is considered to be a well-known and widely consumed beverage and Hunan province is rich in tea plant germplasm.In order to better conserve and utilize Hunan tea plant resources,110 tea accessions from seven geographical origins were used to assess genetic diversity of Hunan tea plant germplasm through genotyping by sequencing(GBS)technology.As a result,a total of 311,044 high-quality single nucleotide polymorphism(SNP)markers were obtained.Population structure,phylogenetic relationships and principal component analysis(PCA)divided the entire accessions into three groups.The genetic diversity and population differentiation analysis showed that the mean observed heterozygosity(Ho)ranged from 0.16 to 0.24,while the mean polymorphic information content(PIC)ranged from 0.14 to 0.17,and mean minor allele frequency(MAF)ranged from 0.11 to 0.14.Analysis of molecular variance(AMOVA)indicated that 81.38%of the total variance was derived from within populations,which suggested a rich genetic diversity in Hunan tea germplasms.Furthermore,a core tea germplasm set was developed,which was comprised of 22 tea plant accessions and maintained the whole genetic diversity of the entire collection.This work should be valuable for conservation and utilization of tea germplasm in Hunan。展开更多
Dear Editor,The tea plant(Camellia sinensis)is an evergreen species.Past breeding efforts have created purple leaf tea varieties with enhanced health benefits,such as‘Zijuan’(C.sinensis var.assa-mica cv.Zijuan[ZJ])(...Dear Editor,The tea plant(Camellia sinensis)is an evergreen species.Past breeding efforts have created purple leaf tea varieties with enhanced health benefits,such as‘Zijuan’(C.sinensis var.assa-mica cv.Zijuan[ZJ])(Figure 1A).To understand the mechanisms underlying the purple trait,the genome of ZJ was assembled using PacBio and Hi-C technologies(Figure 1B).The assembled genome size is approximately 3.06 Gb,comprising 1344 scaffolds with a scaffold N50 size of approximately 214.76 Mb(Supplemental Table 1).In addition,99.12%of the assembled sequences were anchored to 15 chromosomes.展开更多
文摘Hunan is located at the evolutionary transitional zone edge of the Yunnan-Guizhou plateau,where tea germplasms are abundant.At present,although morphological,enzymological and molecular markers have been used to study the genetic relationship of these tea germplasms.However,due to the complexity of the genetic background of tea germplasm resources,the evolution history and taxonomy of Hunan tea germplasm is still unclear.In this study,four Hunan tea landraces from the evolutionary transitional zone were collected,including nine'Chengbu Dongcha'(CBDC),eight'Jianghua Kucha'(JHKC),11'Rucheng Baimao'(RCBM)and eight'Anhua Yuntai'(AHYT).We used whole-genome re-sequencing to identify genetic variation,phylogenetic placement,and population structure among the four tea landraces and then scanned the genome for regions related to population potential selective.Across the samples,we identified an average of 1.38 million(M)SNPs and 6.82×10^(3)INDELs.Based on the high-quality SNPs,we performed neighbor-joining phylogenetic tree,population structure,and principal component analysis,all of which supported that the 36 tea germplasms could been divided into two groups,CBDC and AHYT were clustered into a group,and RCBM and JHKC were clustered into another group.With CBDC,JHKC,and RCBM as control,407,380,and 147 selected genes were identified in AHYT,respectively,were in the top 5%of F_(ST)and Log_(2)^(Ratioθπ)scores.The identified genes are mainly involved in stress tolerance,biosynthesis of important secondary metabolites,adaptability,and yield.These results will be helpful in the classification of tea germplasms and investigating selection–related genes.
基金The Central Government Guides Local Funds(2019XF5041)Hunan Agricultural Science and Technology Innovation Fund(2020CX035)+2 种基金the National Natural Science Foundation of China(32172629,U19A2030,31670689)Provincial Natural Science Foundation of Hunan(2020JJ4358)Hunan Provincial Seed Industry Innovation Project(2021NK1008).
文摘Tea is considered to be a well-known and widely consumed beverage and Hunan province is rich in tea plant germplasm.In order to better conserve and utilize Hunan tea plant resources,110 tea accessions from seven geographical origins were used to assess genetic diversity of Hunan tea plant germplasm through genotyping by sequencing(GBS)technology.As a result,a total of 311,044 high-quality single nucleotide polymorphism(SNP)markers were obtained.Population structure,phylogenetic relationships and principal component analysis(PCA)divided the entire accessions into three groups.The genetic diversity and population differentiation analysis showed that the mean observed heterozygosity(Ho)ranged from 0.16 to 0.24,while the mean polymorphic information content(PIC)ranged from 0.14 to 0.17,and mean minor allele frequency(MAF)ranged from 0.11 to 0.14.Analysis of molecular variance(AMOVA)indicated that 81.38%of the total variance was derived from within populations,which suggested a rich genetic diversity in Hunan tea germplasms.Furthermore,a core tea germplasm set was developed,which was comprised of 22 tea plant accessions and maintained the whole genetic diversity of the entire collection.This work should be valuable for conservation and utilization of tea germplasm in Hunan。
基金supported by the National Natural Science Foundation of China(U20A2045)the Base of Introducing Talents for Tea Plant Biology and Quality Chemistry(D20026)the Science and Technology Project of Yunnan Province(grant no.202102AE090038).
文摘Dear Editor,The tea plant(Camellia sinensis)is an evergreen species.Past breeding efforts have created purple leaf tea varieties with enhanced health benefits,such as‘Zijuan’(C.sinensis var.assa-mica cv.Zijuan[ZJ])(Figure 1A).To understand the mechanisms underlying the purple trait,the genome of ZJ was assembled using PacBio and Hi-C technologies(Figure 1B).The assembled genome size is approximately 3.06 Gb,comprising 1344 scaffolds with a scaffold N50 size of approximately 214.76 Mb(Supplemental Table 1).In addition,99.12%of the assembled sequences were anchored to 15 chromosomes.
