Fermented soy protein isolate with Limosilactobacillus fermentum 606(F-SPI)was investigated for its potential to alleviate senile osteoporosis through modulation of bone metabolism,inflammation,and gut microbiota in a...Fermented soy protein isolate with Limosilactobacillus fermentum 606(F-SPI)was investigated for its potential to alleviate senile osteoporosis through modulation of bone metabolism,inflammation,and gut microbiota in a D-galactose-induced aging rat model.Intraperitoneal administration of Dgalactose led to significant reductions in bone mineral density(BMD),trabecular deterioration,and imbalances in calcium and phosphorus levels,accompanied by increased expression of bone resorption markers and inflammatory cytokines.Oral administration of F-SPI markedly restored bone microarchitecture and cortical integrity,as evidenced by improvements in micro-CT and histological analyses,and increased BMD to levels comparable with alendronate treatment.At the molecular level,F-SPI upregulated intestinal calcium transporters(TRPV6,PMCA1b,NCX1)and renal calcium-regulating genes(TRPV5,CYP27B1),while reducing CYP24A1 expression,suggesting improved calcium absorption and retention.F-SPI also suppressed serum TNF-αand sRANKL levels,indicating reduced osteoclast activity and inflammation.Furthermore,gut microbiota analysis revealed that F-SPI counteracted D-galactoseinduced dysbiosis by enriching SCFA-producing genera such as Bifidobacterium and Faecalibaculum while reducing Clostridium abundance.These changes support the hypothesis that F-SPI exerts bone-protective effects via the gut-bone axis.Overall,these findings suggest that F-SPI mitigates osteoporosis-associated pathology by simultaneously regulating calcium metabolism,inflammatory responses,and gut microbial composition.F-SPI may represent a promising functional food ingredient for managing age-related bone loss.Further studies are warranted to identify the specific bioactive components of F-SPI and validate its efficacy in natural aging models.展开更多
Hair loss is the dysfunction and depletion of hair progenitor cells,leading to hair follicle miniaturization.Oxidative stress and inflammatory responses contribute to hair follicle death,causing hair loss.This study a...Hair loss is the dysfunction and depletion of hair progenitor cells,leading to hair follicle miniaturization.Oxidative stress and inflammatory responses contribute to hair follicle death,causing hair loss.This study aimed to evaluate the hair growth-promoting capacities of Limosilactobacillus fermentum MF10(MF10)and MF10-fermented Goji berries in a depilation mouse model.Both treatments were selected because their anti-oxidative and anti-inflammatory properties have been confirmed in vitro.Black coloration in the shaved skin of mice was apparent from day 12 and was the highest in the MF10-treated group.Until day 19,the highest pigmentation was observed in the MF10-treated group,not significantly different from that observed in the fi-nasteride(Fin)-treated group,the positive control.Notably,hair follicle size increased only by the MF10 treatment.Skin Wnt/β-catenin signaling was activated by MF10 through downregulation of p-GSK3B protein expression and accumulation ofβ-catenin,leading increased serum vascular endothelial growth factor levels only in the MF10 group.Additionally,only MF10 modulated the gut microbial abundance of Duncaniella,which correlated with p-GSK3B protein expression.Overall,our results indicate that L.fermentum MF10-treatment promotes hair growth,and that these effects may be due to gut microbiota activating the skin Wnt/β-catenin signaling.展开更多
基金supported by the Korea Institute of Planning and Evaluation for Technology in Food,Agriculture,Forestry,and Fisheries,funded by the Ministry of Agriculture,Food,and Rural Affairs(RS-2021-IP321036)the Bio-Industry Technology Development Program(20019431,Development of postbiotics based on microbiome for treatment resistant depression and anxiety disorders)funded by the Korea Evaluation Institute of Industrial Technology(KEIT)and the Ministry of Trade Industry&Energy(MOTIE).
文摘Fermented soy protein isolate with Limosilactobacillus fermentum 606(F-SPI)was investigated for its potential to alleviate senile osteoporosis through modulation of bone metabolism,inflammation,and gut microbiota in a D-galactose-induced aging rat model.Intraperitoneal administration of Dgalactose led to significant reductions in bone mineral density(BMD),trabecular deterioration,and imbalances in calcium and phosphorus levels,accompanied by increased expression of bone resorption markers and inflammatory cytokines.Oral administration of F-SPI markedly restored bone microarchitecture and cortical integrity,as evidenced by improvements in micro-CT and histological analyses,and increased BMD to levels comparable with alendronate treatment.At the molecular level,F-SPI upregulated intestinal calcium transporters(TRPV6,PMCA1b,NCX1)and renal calcium-regulating genes(TRPV5,CYP27B1),while reducing CYP24A1 expression,suggesting improved calcium absorption and retention.F-SPI also suppressed serum TNF-αand sRANKL levels,indicating reduced osteoclast activity and inflammation.Furthermore,gut microbiota analysis revealed that F-SPI counteracted D-galactoseinduced dysbiosis by enriching SCFA-producing genera such as Bifidobacterium and Faecalibaculum while reducing Clostridium abundance.These changes support the hypothesis that F-SPI exerts bone-protective effects via the gut-bone axis.Overall,these findings suggest that F-SPI mitigates osteoporosis-associated pathology by simultaneously regulating calcium metabolism,inflammatory responses,and gut microbial composition.F-SPI may represent a promising functional food ingredient for managing age-related bone loss.Further studies are warranted to identify the specific bioactive components of F-SPI and validate its efficacy in natural aging models.
基金supported by Korea University,TOV Co.Ltd,High Value-Added Food Technology Development Program of the Korea Institute of Planning and Evaluation for Technology in Food,Agriculture,Forestry,and Fisheries,and the Ministry for Food,Agriculture,Forestry,and Fisheries of the Republic of Korea[Grant numbers 321036053SB010,1545027002].
文摘Hair loss is the dysfunction and depletion of hair progenitor cells,leading to hair follicle miniaturization.Oxidative stress and inflammatory responses contribute to hair follicle death,causing hair loss.This study aimed to evaluate the hair growth-promoting capacities of Limosilactobacillus fermentum MF10(MF10)and MF10-fermented Goji berries in a depilation mouse model.Both treatments were selected because their anti-oxidative and anti-inflammatory properties have been confirmed in vitro.Black coloration in the shaved skin of mice was apparent from day 12 and was the highest in the MF10-treated group.Until day 19,the highest pigmentation was observed in the MF10-treated group,not significantly different from that observed in the fi-nasteride(Fin)-treated group,the positive control.Notably,hair follicle size increased only by the MF10 treatment.Skin Wnt/β-catenin signaling was activated by MF10 through downregulation of p-GSK3B protein expression and accumulation ofβ-catenin,leading increased serum vascular endothelial growth factor levels only in the MF10 group.Additionally,only MF10 modulated the gut microbial abundance of Duncaniella,which correlated with p-GSK3B protein expression.Overall,our results indicate that L.fermentum MF10-treatment promotes hair growth,and that these effects may be due to gut microbiota activating the skin Wnt/β-catenin signaling.
