【目的】探究PpCLH1基因在叶绿素降解中的功能。果皮叶绿素降解是桃果实成熟过程中的标志性信号之一。叶绿素酶(Chlorophyllase,CLH)一直被人们认为是叶绿素降解过程中的第一种酶,CLH是参与植物叶片和果实褪绿的重要基因。然而,桃CLH基...【目的】探究PpCLH1基因在叶绿素降解中的功能。果皮叶绿素降解是桃果实成熟过程中的标志性信号之一。叶绿素酶(Chlorophyllase,CLH)一直被人们认为是叶绿素降解过程中的第一种酶,CLH是参与植物叶片和果实褪绿的重要基因。然而,桃CLH基因在果皮叶绿素降解中的功能尚不清晰。【方法】以中蟠102为试验材料,克隆PpCLH1基因,分析PpCLH1的氨基酸序列,检测桃果实成熟过程中果皮PpCLH1的转录水平,研究PpCLH1基因在桃叶绿素降解过程中的功能。【结果】PpCLH1编码区全长为972 bp,编码323个氨基酸,在蔷薇科植物中表现出高度的保守性。PpCLH1基因的表达水平随着果实成熟褪绿呈现逐渐上升的趋势,在成熟前12 d达到最高,之后表达量不断降低。瞬时过表达PpCLH1基因后,烟草叶片颜色出现明显褪绿。PpCLH1是桃果皮转色期叶绿素降解的关键基因。【结论】通过对PpCLH1基因进行鉴定和功能研究,发现果实成熟前12 d PpCLH1明显高表达,在烟草叶片中瞬时过表达PpCLH1会导致叶片明显褪绿,这为进一步解析桃果实发育过程中叶绿素降解的分子机制提供了参考。展开更多
Through the continuous development of the acquisition system, the line loss management of the station has been constantly improved. However, due to the influence of various factors, the effect of line loss management ...Through the continuous development of the acquisition system, the line loss management of the station has been constantly improved. However, due to the influence of various factors, the effect of line loss management in station area is not ideal, which brings some problems to the development of the company. This paper analyzes and studies the actual situation of the electricity information acquisition system, the influencing factors of line loss control in the station area, the causes of abnormal line loss in the station area, and the management countermeasures of line loss in the station area based on the electricity information acquisition system.展开更多
文摘【目的】探究PpCLH1基因在叶绿素降解中的功能。果皮叶绿素降解是桃果实成熟过程中的标志性信号之一。叶绿素酶(Chlorophyllase,CLH)一直被人们认为是叶绿素降解过程中的第一种酶,CLH是参与植物叶片和果实褪绿的重要基因。然而,桃CLH基因在果皮叶绿素降解中的功能尚不清晰。【方法】以中蟠102为试验材料,克隆PpCLH1基因,分析PpCLH1的氨基酸序列,检测桃果实成熟过程中果皮PpCLH1的转录水平,研究PpCLH1基因在桃叶绿素降解过程中的功能。【结果】PpCLH1编码区全长为972 bp,编码323个氨基酸,在蔷薇科植物中表现出高度的保守性。PpCLH1基因的表达水平随着果实成熟褪绿呈现逐渐上升的趋势,在成熟前12 d达到最高,之后表达量不断降低。瞬时过表达PpCLH1基因后,烟草叶片颜色出现明显褪绿。PpCLH1是桃果皮转色期叶绿素降解的关键基因。【结论】通过对PpCLH1基因进行鉴定和功能研究,发现果实成熟前12 d PpCLH1明显高表达,在烟草叶片中瞬时过表达PpCLH1会导致叶片明显褪绿,这为进一步解析桃果实发育过程中叶绿素降解的分子机制提供了参考。
文摘Through the continuous development of the acquisition system, the line loss management of the station has been constantly improved. However, due to the influence of various factors, the effect of line loss management in station area is not ideal, which brings some problems to the development of the company. This paper analyzes and studies the actual situation of the electricity information acquisition system, the influencing factors of line loss control in the station area, the causes of abnormal line loss in the station area, and the management countermeasures of line loss in the station area based on the electricity information acquisition system.
