In this study, the in vitro antimicrobial and antiviral activities of the lysozyme from marine strain S-12-86 （LS） were investigated, The antimicrobial activity of LS was tested by minimum inhibition concentration ...In this study, the in vitro antimicrobial and antiviral activities of the lysozyme from marine strain S-12-86 （LS） were investigated, The antimicrobial activity of LS was tested by minimum inhibition concentration （MIC） and minimum bactericidal concentration （MBC） method. The inhibiting effects of LS on pseudo rabies virus （PRV） in swine kidney cells （PK-15 ceils） were judged by cytopathogenic effect test （CPE）, The results showed LS had a broad antimicrobial spectrum against several standard strains including gram-positive bacteria, gram-negative bacteria, fungi, etc, The MIC of LS was 0.25-4.00 mg mL^-1 and its MBC was 0.25-8.00 mg mL^-1, respectively, Observation under the transmission electron microscope revealed that the cell wall of Candida albicans was distorted seriously, and the cytoplasm with many cavities was asymmetrical after being hydrolyzed by LS, The median cytotoxicity concentration （TC50） of LS was 100.0 μg mL^-1, the median effective concentration （EC50） was 0.46 μg mL^-1, and the selectivity index （TI = TC50/EC50） was 217. LS could inhibit PRV in PK-15 cells when it was added to cell culture medium at 0, 2, 4, 6, and 8 h after PK-15 cells had been infected by PRV. From the results, we concluded that LS had broad antimicrobial spectrum and good inhibiting effects on PRV,展开更多
目的本文通过Web of Science核心合集收录自1990年至2019年以来,以“针刺艾灸与穴位”为主题的文献作为数据源对纳入的文献进行可视化分析。方法利用Citespace和Vosviewer软件对该领域研究的发文趋势、国家分布特征、关键词密度和关键...目的本文通过Web of Science核心合集收录自1990年至2019年以来,以“针刺艾灸与穴位”为主题的文献作为数据源对纳入的文献进行可视化分析。方法利用Citespace和Vosviewer软件对该领域研究的发文趋势、国家分布特征、关键词密度和关键词聚类进行分析及文献可视化呈现。结果①针灸刺激穴位的研究文献产量呈上升式增长的趋势,并且增长的爆发性在逐渐增强,该领域逐渐迎来了国际研究发展繁荣期;②国际上研究针灸刺激穴位疗法的国家均形成了一定的合作关系,但是合作强度仍有待加强。其中发文最多的国家为中国;③针灸刺激穴位治疗应对术后恶心呕吐临床随机对照试验研究、针灸刺激穴位的镇痛机制与脑活动荟萃研究、针灸刺激中脘穴的受体机制研究、功能磁共振成像(FMRI)与穴位特异性研究、电针刺激治疗质量与反射抑制的系统性研究和电针刺穴的神经再生与抑制和心动过缓反应研究是该领域主要的研究热点。结论国际范围内对刺灸穴位的研究已具有一定的规模性和层次性,但如何让针灸这传统中医疗法得到更为广泛的推广,仍需对其研究方法的标准性、循证化进行进一步的提高与完善。展开更多
为降低覆冰和舞动等灾害对输电线路造成的破坏,并实时监测电网的运行状态,设计并研制了一种高压输电线路多参数可视化物联监测系统,能够以2 M/s速率高速监测输电线路电流,同时监测线路舞动、环境温湿度等参数。物联监测系统中的各个监...为降低覆冰和舞动等灾害对输电线路造成的破坏,并实时监测电网的运行状态,设计并研制了一种高压输电线路多参数可视化物联监测系统,能够以2 M/s速率高速监测输电线路电流,同时监测线路舞动、环境温湿度等参数。物联监测系统中的各个监测模块内置于导线综合状态监测球,对输电线路进行2 M/s高速录波的同时,通过归一化电流差值识别算法进行电流故障识别,将故障前后的10个周期的录波数据传出。利用全球定位系统(global position system,GPS)秒脉冲(pulse per second,PPS)信号实现全电网不同终端的高速采样信号严格对齐,时间分辨率达到了100 ns,确保了高速录波数据的时间一致性。高压线路试验结果表明:该装置可实时高速录波和进行电流故障诊断,利用多参数综合监测输电线路的运行状态。展开更多
文摘In this study, the in vitro antimicrobial and antiviral activities of the lysozyme from marine strain S-12-86 （LS） were investigated, The antimicrobial activity of LS was tested by minimum inhibition concentration （MIC） and minimum bactericidal concentration （MBC） method. The inhibiting effects of LS on pseudo rabies virus （PRV） in swine kidney cells （PK-15 ceils） were judged by cytopathogenic effect test （CPE）, The results showed LS had a broad antimicrobial spectrum against several standard strains including gram-positive bacteria, gram-negative bacteria, fungi, etc, The MIC of LS was 0.25-4.00 mg mL^-1 and its MBC was 0.25-8.00 mg mL^-1, respectively, Observation under the transmission electron microscope revealed that the cell wall of Candida albicans was distorted seriously, and the cytoplasm with many cavities was asymmetrical after being hydrolyzed by LS, The median cytotoxicity concentration （TC50） of LS was 100.0 μg mL^-1, the median effective concentration （EC50） was 0.46 μg mL^-1, and the selectivity index （TI = TC50/EC50） was 217. LS could inhibit PRV in PK-15 cells when it was added to cell culture medium at 0, 2, 4, 6, and 8 h after PK-15 cells had been infected by PRV. From the results, we concluded that LS had broad antimicrobial spectrum and good inhibiting effects on PRV,
文摘目的本文通过Web of Science核心合集收录自1990年至2019年以来,以“针刺艾灸与穴位”为主题的文献作为数据源对纳入的文献进行可视化分析。方法利用Citespace和Vosviewer软件对该领域研究的发文趋势、国家分布特征、关键词密度和关键词聚类进行分析及文献可视化呈现。结果①针灸刺激穴位的研究文献产量呈上升式增长的趋势,并且增长的爆发性在逐渐增强,该领域逐渐迎来了国际研究发展繁荣期;②国际上研究针灸刺激穴位疗法的国家均形成了一定的合作关系,但是合作强度仍有待加强。其中发文最多的国家为中国;③针灸刺激穴位治疗应对术后恶心呕吐临床随机对照试验研究、针灸刺激穴位的镇痛机制与脑活动荟萃研究、针灸刺激中脘穴的受体机制研究、功能磁共振成像(FMRI)与穴位特异性研究、电针刺激治疗质量与反射抑制的系统性研究和电针刺穴的神经再生与抑制和心动过缓反应研究是该领域主要的研究热点。结论国际范围内对刺灸穴位的研究已具有一定的规模性和层次性,但如何让针灸这传统中医疗法得到更为广泛的推广,仍需对其研究方法的标准性、循证化进行进一步的提高与完善。
文摘为降低覆冰和舞动等灾害对输电线路造成的破坏,并实时监测电网的运行状态,设计并研制了一种高压输电线路多参数可视化物联监测系统,能够以2 M/s速率高速监测输电线路电流,同时监测线路舞动、环境温湿度等参数。物联监测系统中的各个监测模块内置于导线综合状态监测球,对输电线路进行2 M/s高速录波的同时,通过归一化电流差值识别算法进行电流故障识别,将故障前后的10个周期的录波数据传出。利用全球定位系统(global position system,GPS)秒脉冲(pulse per second,PPS)信号实现全电网不同终端的高速采样信号严格对齐,时间分辨率达到了100 ns,确保了高速录波数据的时间一致性。高压线路试验结果表明:该装置可实时高速录波和进行电流故障诊断,利用多参数综合监测输电线路的运行状态。
