Bacillus cereus is a significant pathogen responsible for toxin-induced foodborne diseases,and is widely distributed in the natural environment.B.cereus biofilm formation is regulated by multifactorial interactions,de...Bacillus cereus is a significant pathogen responsible for toxin-induced foodborne diseases,and is widely distributed in the natural environment.B.cereus biofilm formation is regulated by multifactorial interactions,demonstrating dynamic adaptability to environmental cues.In this study,crystal violet staining and MTT staining methods were employed to conduct an indepth investigation into the biofilm formation and metabolic activity changes of five strains of B.cereus in different culture media and with various culture conditions.Experimental data indicated that the composition of the culture medium,the length of incubation time,and the fluctuation of temperature all had a significant impact on the biofilm formation of B.cereus,and this impact exhibited obvious differences among different strains.This result provides a solid experimental basis for deeply elucidating the internal regulatory mechanism of B.cereus biofilm formation and the change principles of metabolic activity.展开更多
基金supported by the National Key R&D Pro-gram of China(No.2022YFD1301003)the Shandong Modern Agricultural Technology and Industry System(No.SDAIT-11-11)+4 种基金the Marine Science and Technology Innovation Project of Qingdao(No.24-1-3-hygg-25-hy)the Cooperative Scientific Research Project‘Chunhui Plan’of the Ministry of Education of PRC(No.HZKY 20220481)the Open Foundation of State Key Laboratory of Marine Food Processing&Safety Control(No.SKL202408)the Shandong Province Grant for Distin-guished Young Scholars(No.ZR2022JQ15)the Young Taishan Scholars Program of Shandong Province(No.tsqn202103094)。
文摘Bacillus cereus is a significant pathogen responsible for toxin-induced foodborne diseases,and is widely distributed in the natural environment.B.cereus biofilm formation is regulated by multifactorial interactions,demonstrating dynamic adaptability to environmental cues.In this study,crystal violet staining and MTT staining methods were employed to conduct an indepth investigation into the biofilm formation and metabolic activity changes of five strains of B.cereus in different culture media and with various culture conditions.Experimental data indicated that the composition of the culture medium,the length of incubation time,and the fluctuation of temperature all had a significant impact on the biofilm formation of B.cereus,and this impact exhibited obvious differences among different strains.This result provides a solid experimental basis for deeply elucidating the internal regulatory mechanism of B.cereus biofilm formation and the change principles of metabolic activity.
