目的对红花注射液进行化学成分分析及应用分子对接技术筛选红花注射液中过敏原成分。方法应用高效液相色谱-离子阱-飞行时间质谱高分辨质谱技术(high performance liquid chromatography-ion trap-time-of-flight high resolution mass ...目的对红花注射液进行化学成分分析及应用分子对接技术筛选红花注射液中过敏原成分。方法应用高效液相色谱-离子阱-飞行时间质谱高分辨质谱技术(high performance liquid chromatography-ion trap-time-of-flight high resolution mass spectrometry,HPLC-IT-TOF-MS)鉴定红花注射液中化学成分,应用分子对接技术进一步对潜在过敏原成分进行筛选。结果从红花注射液中共鉴定出40个化合物,其中26个查耳酮碳苷类化合物、8个黄酮类化合物、3个生物碱类化合物和3个其他类化合物,所有化合物均在负离子模式下鉴定。将这些化合物分别与人血清白蛋白(human serum albumin,HSA)、Mas相关G蛋白偶联受体X2(Mas-related G protein-coupled receptor X2,MRGPRX2)、Ras同源基因家族成员A(Ras homolog gene family member A)等蛋白进行分子对接,其中与HAS结合的化合物6个,与MRGPRX2结合的化合物有9个,发现红花注射液中过敏成分主要为6-羟基山柰酚类化合物和红花醌类化合物。结论建立了液-质联用与分子对接相结合的过敏原筛选方法,从红花注射液中鉴定出40个化合物,明确6-羟基山柰酚类和红花醌类化合物为潜在过敏原,为红花注射液过敏原筛选及其致敏机制研究提供了重要参考和数据。展开更多
The existence of the Cretaceous-Tertiary （K/T） boundary in the non-marine succession is expected at Jiayin in the Heilongjiang River area, China. Zircons from a tuff sample from the Baishantou Member of Wuyun Format...The existence of the Cretaceous-Tertiary （K/T） boundary in the non-marine succession is expected at Jiayin in the Heilongjiang River area, China. Zircons from a tuff sample from the Baishantou Member of Wuyun Formation in Jiayin were analyzed by the laser ablation inductively coupled plasma mass spectrometry （LA-ICP-MS） U-Pb dating and fission-track dating methods. Ages of 64.1±0.7 Ma （U-Pb） and 61.7±1.8 Ma （fission-track dating） were obtained, which allow reevaluation of a previously reported late Maastrichian age for the tuff layer that was in conflict with the paleontological evidence. These results confirm the Danian age of the section in agreement with the paleontological evidence.展开更多
Recent geological and paleontological exploration in the Indus basin of Pakistan allowed the discoveries of numerous remains of non-marine reptiles( titanosaurian sauropod,abelisaurian and noasaurian theropod dinosaur...Recent geological and paleontological exploration in the Indus basin of Pakistan allowed the discoveries of numerous remains of non-marine reptiles( titanosaurian sauropod,abelisaurian and noasaurian theropod dinosaurs),and marine reptiles( crocodiles),flying reptiles( pterosaurs),marine and non-marine mammals,fishes,invertebrates,and plants,especially Pakistan is relatively rich in footprints / trackways in the Mesozoic.These vertebrates of Indo-Pakistan are very significant for paleobiogeographic study due to the present-day connection of this continent with Asia in Northern Hemisphere,whereas during past( Jurassic and pre-Jurassic) it was connected to the Gondwana. The Mesozoic vertebrates show close affinities with Gondwanan landmasses.The Cenozoic vertebrates show Eurasian affinity and migrated from Indo-Pak subcontinent to Eurasia or vice versa via Paleo Indus River systems along Western Indus Suture,after long journey of about 6 000 km the first collision of Indo-Pak subcontinent with Asia occurred at terminal Cretaceous.展开更多
文摘目的对红花注射液进行化学成分分析及应用分子对接技术筛选红花注射液中过敏原成分。方法应用高效液相色谱-离子阱-飞行时间质谱高分辨质谱技术(high performance liquid chromatography-ion trap-time-of-flight high resolution mass spectrometry,HPLC-IT-TOF-MS)鉴定红花注射液中化学成分,应用分子对接技术进一步对潜在过敏原成分进行筛选。结果从红花注射液中共鉴定出40个化合物,其中26个查耳酮碳苷类化合物、8个黄酮类化合物、3个生物碱类化合物和3个其他类化合物,所有化合物均在负离子模式下鉴定。将这些化合物分别与人血清白蛋白(human serum albumin,HSA)、Mas相关G蛋白偶联受体X2(Mas-related G protein-coupled receptor X2,MRGPRX2)、Ras同源基因家族成员A(Ras homolog gene family member A)等蛋白进行分子对接,其中与HAS结合的化合物6个,与MRGPRX2结合的化合物有9个,发现红花注射液中过敏成分主要为6-羟基山柰酚类化合物和红花醌类化合物。结论建立了液-质联用与分子对接相结合的过敏原筛选方法,从红花注射液中鉴定出40个化合物,明确6-羟基山柰酚类和红花醌类化合物为潜在过敏原,为红花注射液过敏原筛选及其致敏机制研究提供了重要参考和数据。
基金the Project NSFC No30220130698the Project "111" of China for their support the National Science Council,Taiwan(grant 97-2811-M- 002-040)
文摘The existence of the Cretaceous-Tertiary （K/T） boundary in the non-marine succession is expected at Jiayin in the Heilongjiang River area, China. Zircons from a tuff sample from the Baishantou Member of Wuyun Formation in Jiayin were analyzed by the laser ablation inductively coupled plasma mass spectrometry （LA-ICP-MS） U-Pb dating and fission-track dating methods. Ages of 64.1±0.7 Ma （U-Pb） and 61.7±1.8 Ma （fission-track dating） were obtained, which allow reevaluation of a previously reported late Maastrichian age for the tuff layer that was in conflict with the paleontological evidence. These results confirm the Danian age of the section in agreement with the paleontological evidence.
文摘Recent geological and paleontological exploration in the Indus basin of Pakistan allowed the discoveries of numerous remains of non-marine reptiles( titanosaurian sauropod,abelisaurian and noasaurian theropod dinosaurs),and marine reptiles( crocodiles),flying reptiles( pterosaurs),marine and non-marine mammals,fishes,invertebrates,and plants,especially Pakistan is relatively rich in footprints / trackways in the Mesozoic.These vertebrates of Indo-Pakistan are very significant for paleobiogeographic study due to the present-day connection of this continent with Asia in Northern Hemisphere,whereas during past( Jurassic and pre-Jurassic) it was connected to the Gondwana. The Mesozoic vertebrates show close affinities with Gondwanan landmasses.The Cenozoic vertebrates show Eurasian affinity and migrated from Indo-Pak subcontinent to Eurasia or vice versa via Paleo Indus River systems along Western Indus Suture,after long journey of about 6 000 km the first collision of Indo-Pak subcontinent with Asia occurred at terminal Cretaceous.
