目的探讨Mex3C-1对卡巴胆碱诱导的Fos表达的影响。方法将具有可持续表达人Mex3C-1序列的质粒pLV-CMV-Mex3C(OE)在HEK293T细胞中包装成慢病毒载体,同时制备非特异性对照(NC)CmiR0001-MR03的慢病毒载体,分别感染人神经母细胞瘤细胞SH-SY5Y...目的探讨Mex3C-1对卡巴胆碱诱导的Fos表达的影响。方法将具有可持续表达人Mex3C-1序列的质粒pLV-CMV-Mex3C(OE)在HEK293T细胞中包装成慢病毒载体,同时制备非特异性对照(NC)CmiR0001-MR03的慢病毒载体,分别感染人神经母细胞瘤细胞SH-SY5Y,经嘌呤霉素筛选后得到稳定高表达Mex3C-1和阴性对照细胞系,用Real time PCR和Western blot方法检测Mex3C-1的基因和蛋白表达效果。之后用卡巴胆碱诱导Fos表达,在诱导0、30、60、90和120min后分别提取mRNA,采用Real time PCR方法检测Fos mRNA的相对表达量。结果Real time PCR检测结果显示,OE组的Mex3C-1 mRNA的相对表达量(21.11±0.60)高于NC组(1.03±0.13)(t=32.63,P=0.000)。Western blot结果显示,在82kDa处OE组的Mex3C-1蛋白表达量高于NC组(P<0.001)。Real time PCR检测不同干预时间两组Fos mRNA,除0min外各个时间点OE组均高于NC组,表明Mex3C-1的过表达可以明显上调Fos mRNA的表达;NC组于120min时已基本恢复至基础值,而OE组120min时Fos mRNA表达量仍然较高,Mex3C-1过表达可以延长Fos mRNA的半衰期,增强其稳定性,OE组与阴性对照NC组比较,Fos mRNA表达量差异有统计学意义(F=287.069,P=0.000)。结论持续过表达Mex3C-1的人神经母细胞瘤SH-SY5Y细胞系建立成功,且Mex3C-1过表达能够明显增强卡巴胆碱诱导的Fos表达程度并增强其稳定性。展开更多
The 3D P-wave velocity structure beneath the South China Block was determined by applying arrival times from 269 teleseismic events recorded by 240 seismic stations within the study region. Our tomographic results rev...The 3D P-wave velocity structure beneath the South China Block was determined by applying arrival times from 269 teleseismic events recorded by 240 seismic stations within the study region. Our tomographic results reveal the deep structural characteristics of major tectonic units and ore concentration areas. There are distinct high velocity anomalies beneath the ancient Yangtze and Cathaysia blocks, with the lithosphere of the Cathaysia Block being thinner than the Yangtze Block;the Jiangnan orogenic belt, located in the combined zone of two blocks, is a high and low velocity anomaly conversion zone;the famous metallogenic belts of Edongnan, the Youjiang Basin and the Cathaysia Block are obviously low velocity areas with different metallogenic mechanisms. The deep ore-forming material source in the Edongnan metallogenic belt is different from that of the Cathaysia Block. The low velocity anomaly under the Cathaysia Block related to mineralization results from the upwelling of mantle material, caused by the joint action of the Paleo-Tethys tectonic domain, the Paleo-Pacific tectonic domain and the Hainan mantle plume migration and erosion, which has been occurring from northeast to southwest since 80 Ma. The low-temperature mineralization mechanism of Youjiang Basin should be considered not only in terms of the influence of the Emeishan mantle plume in the west and the Paleo-Tethys tectonic domain in the south, but also in the context of the influence of the upwelling of asthenospheric material from the PaleoPacific tectonic domain in the east.展开更多
文摘目的探讨Mex3C-1对卡巴胆碱诱导的Fos表达的影响。方法将具有可持续表达人Mex3C-1序列的质粒pLV-CMV-Mex3C(OE)在HEK293T细胞中包装成慢病毒载体,同时制备非特异性对照(NC)CmiR0001-MR03的慢病毒载体,分别感染人神经母细胞瘤细胞SH-SY5Y,经嘌呤霉素筛选后得到稳定高表达Mex3C-1和阴性对照细胞系,用Real time PCR和Western blot方法检测Mex3C-1的基因和蛋白表达效果。之后用卡巴胆碱诱导Fos表达,在诱导0、30、60、90和120min后分别提取mRNA,采用Real time PCR方法检测Fos mRNA的相对表达量。结果Real time PCR检测结果显示,OE组的Mex3C-1 mRNA的相对表达量(21.11±0.60)高于NC组(1.03±0.13)(t=32.63,P=0.000)。Western blot结果显示,在82kDa处OE组的Mex3C-1蛋白表达量高于NC组(P<0.001)。Real time PCR检测不同干预时间两组Fos mRNA,除0min外各个时间点OE组均高于NC组,表明Mex3C-1的过表达可以明显上调Fos mRNA的表达;NC组于120min时已基本恢复至基础值,而OE组120min时Fos mRNA表达量仍然较高,Mex3C-1过表达可以延长Fos mRNA的半衰期,增强其稳定性,OE组与阴性对照NC组比较,Fos mRNA表达量差异有统计学意义(F=287.069,P=0.000)。结论持续过表达Mex3C-1的人神经母细胞瘤SH-SY5Y细胞系建立成功,且Mex3C-1过表达能够明显增强卡巴胆碱诱导的Fos表达程度并增强其稳定性。
基金supported by Shanghai Sheshan National Geophysical Observatory(Grant No.2020k07)the Chinese Geological Survey(Grant Nos.DD20190448,DD20190370)+1 种基金the National Natural Science Foundation of China(Grant Nos.41374101,42174172)the National Key Research and Development Program of China(Grant No.2019YFA0708601)。
文摘The 3D P-wave velocity structure beneath the South China Block was determined by applying arrival times from 269 teleseismic events recorded by 240 seismic stations within the study region. Our tomographic results reveal the deep structural characteristics of major tectonic units and ore concentration areas. There are distinct high velocity anomalies beneath the ancient Yangtze and Cathaysia blocks, with the lithosphere of the Cathaysia Block being thinner than the Yangtze Block;the Jiangnan orogenic belt, located in the combined zone of two blocks, is a high and low velocity anomaly conversion zone;the famous metallogenic belts of Edongnan, the Youjiang Basin and the Cathaysia Block are obviously low velocity areas with different metallogenic mechanisms. The deep ore-forming material source in the Edongnan metallogenic belt is different from that of the Cathaysia Block. The low velocity anomaly under the Cathaysia Block related to mineralization results from the upwelling of mantle material, caused by the joint action of the Paleo-Tethys tectonic domain, the Paleo-Pacific tectonic domain and the Hainan mantle plume migration and erosion, which has been occurring from northeast to southwest since 80 Ma. The low-temperature mineralization mechanism of Youjiang Basin should be considered not only in terms of the influence of the Emeishan mantle plume in the west and the Paleo-Tethys tectonic domain in the south, but also in the context of the influence of the upwelling of asthenospheric material from the PaleoPacific tectonic domain in the east.
