A cytotoxicity study based on keratinocytes was carried out to determine the safe dosing concentration of plant extract formulation(hereinafter referred to as the MOSOGY).On this basis,the 3D epidermal skin model(Epik...A cytotoxicity study based on keratinocytes was carried out to determine the safe dosing concentration of plant extract formulation(hereinafter referred to as the MOSOGY).On this basis,the 3D epidermal skin model(Epikutis^(®),which is abbreviated as Epikutis later)was stimulated with anionic surfactant sodium lauryl sulfate(SLS)was used to construct an in vitro 3D epidermal skin injury model and detect the proinflammatory factor(IL-1α)after MOSOGY acted on the 3D epidermal skin model.Additionally,capsaicin(CAP)was used to stimulate the 3D epidermal skin model(EpiKutis)to detect changes in the content of transient receptor potential vanilloid 1(TRPV1)after MOSOGY treatment.The hydration levels of the stratum corneum were tested using the 3D epidermal skin model.A hyaluronidase(HAase)inhibition experiment was conducted to assess the soothing effect of BSBE.The experimental results showed that MOSOGY exhibited no significant cytotoxicity within the concentration range of 0~2.50%At a concentration of 2.00%,the inhibition rates of IL-1αand PGE2 were 15.71%and 19.00%respectively.At a concentration of 5.00%,MOSOGY exhibited inhibition rates of 43.76%for TRPV1 and 11.11%for IL-1α.MOSOGY at concentrations of 1.00%and 5.00%showed inhibition rates of 76.50%and 86.50%for HAase,respectively.The moisturizing efficacy test showed that at the concentration of 5.00%,the water content of the stratum corneum of the 3D epidermal skin model increased by 31.91%.In addition,the 3D epidermal skin model used in this experiment has the characteristics of layered structure,physiological and metabolic functions that are highly similar to natural human skin.The above research shows that MOSOGY at a certain concentration effectively inhibits IL-1α,PGE2,TRPV1 and HAase in human skin,especially the inhibition rate of HAase is more than 70.00%,indicating that BSBE have strong anti-allergic effect,and skin hydration of the 3D epidermal skin model also has a significant increase.The results indicate that MOSOGY exhibits inhibitory effects on IL-1α,PGE2,TRPV1,and HAase,achieving significant soothing and reparative effects through the inhibition of multiple targets.Additionally,it demonstrates good moisturizing efficacy and safety,making it suitable for use in cosmetics with corresponding efficacy claims.展开更多
A membrane aerated biofilm reactor is a promising technology for wastewater treatment. In this study, a carbon-membrane aerated biofilm reactor （CMABR） has been developed, to remove carbon organics and nitrogen simu...A membrane aerated biofilm reactor is a promising technology for wastewater treatment. In this study, a carbon-membrane aerated biofilm reactor （CMABR） has been developed, to remove carbon organics and nitrogen simultaneously from one reactor. The results showed that CMABR has a high chemical oxygen demand （COD） and nitrogen removal efficiency, as it is operated with a hydraulic retention time （HRT） of 20 h, and it also showed a perfect performance, even if the HRT was shortened to 12 h. In this period, the removal efficiencies of COD, ammonia nitrogen （NH4^＋-N）, and total nitrogen （TN） reached 86%, 94%, and 84%, respectively. However, the removal efficiencies of NH4^＋-N and TN declined rapidly as the HRT was shortened to 8 h. This is because of the excessive growth of biomass on the nonwoven fiber and very high organic loading rate. The fluorescence in situ hybridization （FISH） analysis indicated that the ammonia oxidizing bacteria （AOB） were mainly distributed in the inner layer of the biofilm. The coexistence of AOB and eubacteria in one biofilm can enhance the simultaneous removal of COD and nitrogen.展开更多
文摘A cytotoxicity study based on keratinocytes was carried out to determine the safe dosing concentration of plant extract formulation(hereinafter referred to as the MOSOGY).On this basis,the 3D epidermal skin model(Epikutis^(®),which is abbreviated as Epikutis later)was stimulated with anionic surfactant sodium lauryl sulfate(SLS)was used to construct an in vitro 3D epidermal skin injury model and detect the proinflammatory factor(IL-1α)after MOSOGY acted on the 3D epidermal skin model.Additionally,capsaicin(CAP)was used to stimulate the 3D epidermal skin model(EpiKutis)to detect changes in the content of transient receptor potential vanilloid 1(TRPV1)after MOSOGY treatment.The hydration levels of the stratum corneum were tested using the 3D epidermal skin model.A hyaluronidase(HAase)inhibition experiment was conducted to assess the soothing effect of BSBE.The experimental results showed that MOSOGY exhibited no significant cytotoxicity within the concentration range of 0~2.50%At a concentration of 2.00%,the inhibition rates of IL-1αand PGE2 were 15.71%and 19.00%respectively.At a concentration of 5.00%,MOSOGY exhibited inhibition rates of 43.76%for TRPV1 and 11.11%for IL-1α.MOSOGY at concentrations of 1.00%and 5.00%showed inhibition rates of 76.50%and 86.50%for HAase,respectively.The moisturizing efficacy test showed that at the concentration of 5.00%,the water content of the stratum corneum of the 3D epidermal skin model increased by 31.91%.In addition,the 3D epidermal skin model used in this experiment has the characteristics of layered structure,physiological and metabolic functions that are highly similar to natural human skin.The above research shows that MOSOGY at a certain concentration effectively inhibits IL-1α,PGE2,TRPV1 and HAase in human skin,especially the inhibition rate of HAase is more than 70.00%,indicating that BSBE have strong anti-allergic effect,and skin hydration of the 3D epidermal skin model also has a significant increase.The results indicate that MOSOGY exhibits inhibitory effects on IL-1α,PGE2,TRPV1,and HAase,achieving significant soothing and reparative effects through the inhibition of multiple targets.Additionally,it demonstrates good moisturizing efficacy and safety,making it suitable for use in cosmetics with corresponding efficacy claims.
基金This work was supported by the National Natural Science Foundation of China （No. 50578023）。
文摘A membrane aerated biofilm reactor is a promising technology for wastewater treatment. In this study, a carbon-membrane aerated biofilm reactor （CMABR） has been developed, to remove carbon organics and nitrogen simultaneously from one reactor. The results showed that CMABR has a high chemical oxygen demand （COD） and nitrogen removal efficiency, as it is operated with a hydraulic retention time （HRT） of 20 h, and it also showed a perfect performance, even if the HRT was shortened to 12 h. In this period, the removal efficiencies of COD, ammonia nitrogen （NH4^＋-N）, and total nitrogen （TN） reached 86%, 94%, and 84%, respectively. However, the removal efficiencies of NH4^＋-N and TN declined rapidly as the HRT was shortened to 8 h. This is because of the excessive growth of biomass on the nonwoven fiber and very high organic loading rate. The fluorescence in situ hybridization （FISH） analysis indicated that the ammonia oxidizing bacteria （AOB） were mainly distributed in the inner layer of the biofilm. The coexistence of AOB and eubacteria in one biofilm can enhance the simultaneous removal of COD and nitrogen.
