以牡丹籽壳为原料,利用大孔树脂和C18反相键合硅胶柱(C18柱)联合分离短叶松素,回收率为(98.33±0.64)%。用70%(体积分数)乙醇提取牡丹籽壳粗黄酮(Mu Dan Ke Flavonoids,MDKF),得率最高为(10.54±0.13)%;比较了6种大孔树脂(AB-8...以牡丹籽壳为原料,利用大孔树脂和C18反相键合硅胶柱(C18柱)联合分离短叶松素,回收率为(98.33±0.64)%。用70%(体积分数)乙醇提取牡丹籽壳粗黄酮(Mu Dan Ke Flavonoids,MDKF),得率最高为(10.54±0.13)%;比较了6种大孔树脂(AB-8、S-8、DM301、HPD600、HPD100和D101)的吸附率和解析率,发现S-8大孔树脂的吸附率和解析率最佳,分别为83.47%和84.46%;优化S-8大孔树脂分离MDKF的最佳条件为:上样液质量浓度1.6 mg/mL,上样液流速2.0 mL/min,洗脱剂乙醇体积分数60%,洗脱液流速1.5 mL/min,洗脱液体积100 mL;对经过C18柱分离后的组分进行LC-MS分析得到短叶松素,回收率为(98.33±0.64)%;分离前后抗氧化能力比较:C18纯化物>S-8大孔树脂纯化物>MDKF粗提物;分子对接实验表明,短叶松素与超氧化物歧化酶、过氧化氢酶、过氧化物酶、谷胱甘肽过氧化物酶均有结合能力,过氧化氢酶的结合能力最强为-9.1 kcal/mol。实验表明,短叶松素具有较好的抗氧化能力,可作为食品、药品或化妆品等抗氧化剂的添加,应用前景非常广泛,对牡丹籽壳的进一步开发利用提供理论支持。展开更多
Two sediment cores were retrieved in the hadal zone of the Yap Trench,and their concentrations of six major elements Mg,Al,Ca,Ti,Mn and Fe and nine trace elements Sr,Ba,Pb,V,Cr,Co,Ni,Cu and Zn were determined in induc...Two sediment cores were retrieved in the hadal zone of the Yap Trench,and their concentrations of six major elements Mg,Al,Ca,Ti,Mn and Fe and nine trace elements Sr,Ba,Pb,V,Cr,Co,Ni,Cu and Zn were determined in inductively coupled plasma atomic emission spectrometry(ICP-AES).According to the vertical distribution profiles of the 15 elements,the correlation of their concentrations,the ratios of Ni/Co,V/Cr,Fe/Al,and Ti/Al,and morphological characteristics of the sediment samples,the implications of the depositional environment and the sediment provenance were analyzed.The results show that the ratio of Ni/Co in all depths of the two sediment cores were below 5,and the ratio of V/Cr were lower than 2,indicating that the depositional environment of the hadal zone in the trench was oxidative and might have inflow of the Antarctic bottom oxygen-rich water.The sediment samples on the eastern side of the trench were siliceous mud mainly composed of diatoms,radiolarian,and sponge needles from surface to deep layer.The vertical profile of the elements,the concentration of TOC and the fossil record indicated that the sediment sample from station Dive113 was well mixed from surface to bottom layer.Based on the correlation of concentrations of the elements,the morphological characteristics of the sediment,and the ratios of Fe/Al and Ti/Al,we inferred that the sediment in the hadal zone of the trench had terrestrial,volcanic,biological,and authigenic sources.Major source of the sediment in the eastern side of the trench were terrestrial;whereas the sediment in the western side of the trench received more volcaniclastic input.展开更多
In this study,cored sediment samples collected by the Jiaolong Submersible at 6779 m depth from the hadal zone of the Yap Trench in May 2016 were sliced in 1-cm interval from top to bottom,and lipids in each sediment ...In this study,cored sediment samples collected by the Jiaolong Submersible at 6779 m depth from the hadal zone of the Yap Trench in May 2016 were sliced in 1-cm interval from top to bottom,and lipids in each sediment layer were quantitatively and qualitatively analyzed.The vertical distribution profiles of the lipids in the sediment sample,their main existing forms,and their possible sources were investigated.The results show that the concentration of lipid in the surface sediment was the highest with the carbon number from 12 to 27,dominated by medium and short-chain lipids.The total concentration of fatty acids in surface sediment was much higher than those in the offshore and deep-sea areas,being up to 325.77μg/g due to the funnel effect caused by the“V”terrain of the trench.Fatty acid 18:0 was the most abundant lipids in the sediment sample.Abnormal high concentrations of fatty acid 18:1ω7 and alkanes indicated the existence of hydrothermal fluids in the study area.In addition,saturated fatty acids and monounsaturated fatty acids existed mainly in free form,and polyunsaturated fatty acids existed mainly in bound form.Most of the alkanes were in bound form,and their major source was autochthounous input.The carbon number of alcohols in the sediment sample ranged from 12 to 20,mainly existed in bound form.The source of fatty acids was mainly autochthonous input,and the neutral lipids had both marine and terrestrial origin.This is the first study of lipids in hadal sediment of the Yap Trench.The results will promote deeper understanding of organic carbon cycle in marine environment.展开更多
背景:针对颈椎后路单开门椎管扩大成形术,C3椎板2种处理方式(C3椎板切除与C3椎板成形)的临床效果以及对术后颈椎功能的影响如何,相关客观评价较少,为此进行了此项研究。目的:系统评价颈椎后路椎管扩大成形术中C3椎板切除与C3椎板成形治...背景:针对颈椎后路单开门椎管扩大成形术,C3椎板2种处理方式(C3椎板切除与C3椎板成形)的临床效果以及对术后颈椎功能的影响如何,相关客观评价较少,为此进行了此项研究。目的:系统评价颈椎后路椎管扩大成形术中C3椎板切除与C3椎板成形治疗多节段脊髓型颈椎病的疗效。方法:计算机检索Cochrane Central、PubMed、EMbase、the ISI Web of Knowledge Database、CNKI、CMB、维普、万方数据库,检索时间均为建库时间至2018-07-01。纳入关于C3椎板切除和椎板成形治疗多节段脊髓型颈椎病疗效对比的随机或非随机对照试验。Meta分析采用Cochrane协作网提供的Rev-Man5.3软件进行。结果与结论:①共纳入5个研究,316例患者,其中C3椎板切除组146例,C3椎板成形组170例;②Mate分析结果显示,在术后颈椎功能方面,与传统C3椎板成形相比,C3椎板切除术后颈椎活动度丢失较少[SMD=-5.89,95%CI(-7.25,-4.70),P <0.05]、术后轴性症状发生率低[SMD=-0.23,95%CI(0.12,0.46),P<0.05];③在神经功能恢复方面,2组术后日本骨科协会评分差异无显著性意义[SMD=-0.02,95%CI(-0.55,0.51),P=0.95> 0.05];④结果表明,采用椎管扩大成形治疗多节段脊髓型颈椎病时C3椎板的2种处理方式(椎板切除或椎板成形)均能取得较好的临床效果,但椎板切除能有效防止颈椎活动度丢失、降低术后轴性症状发生率。展开更多
文摘以牡丹籽壳为原料,利用大孔树脂和C18反相键合硅胶柱(C18柱)联合分离短叶松素,回收率为(98.33±0.64)%。用70%(体积分数)乙醇提取牡丹籽壳粗黄酮(Mu Dan Ke Flavonoids,MDKF),得率最高为(10.54±0.13)%;比较了6种大孔树脂(AB-8、S-8、DM301、HPD600、HPD100和D101)的吸附率和解析率,发现S-8大孔树脂的吸附率和解析率最佳,分别为83.47%和84.46%;优化S-8大孔树脂分离MDKF的最佳条件为:上样液质量浓度1.6 mg/mL,上样液流速2.0 mL/min,洗脱剂乙醇体积分数60%,洗脱液流速1.5 mL/min,洗脱液体积100 mL;对经过C18柱分离后的组分进行LC-MS分析得到短叶松素,回收率为(98.33±0.64)%;分离前后抗氧化能力比较:C18纯化物>S-8大孔树脂纯化物>MDKF粗提物;分子对接实验表明,短叶松素与超氧化物歧化酶、过氧化氢酶、过氧化物酶、谷胱甘肽过氧化物酶均有结合能力,过氧化氢酶的结合能力最强为-9.1 kcal/mol。实验表明,短叶松素具有较好的抗氧化能力,可作为食品、药品或化妆品等抗氧化剂的添加,应用前景非常广泛,对牡丹籽壳的进一步开发利用提供理论支持。
基金Supported by the National Basic Research Program of China(973 Program)(No.2015CB755904)the National Natural Science Foundation of China(No.41676067)+1 种基金the Fundamental Funds for Central Universities,China(No.201762030)the Overseas Expertise Introduction Project for Discipline Innovation(111 Project)(No.B13030)。
文摘Two sediment cores were retrieved in the hadal zone of the Yap Trench,and their concentrations of six major elements Mg,Al,Ca,Ti,Mn and Fe and nine trace elements Sr,Ba,Pb,V,Cr,Co,Ni,Cu and Zn were determined in inductively coupled plasma atomic emission spectrometry(ICP-AES).According to the vertical distribution profiles of the 15 elements,the correlation of their concentrations,the ratios of Ni/Co,V/Cr,Fe/Al,and Ti/Al,and morphological characteristics of the sediment samples,the implications of the depositional environment and the sediment provenance were analyzed.The results show that the ratio of Ni/Co in all depths of the two sediment cores were below 5,and the ratio of V/Cr were lower than 2,indicating that the depositional environment of the hadal zone in the trench was oxidative and might have inflow of the Antarctic bottom oxygen-rich water.The sediment samples on the eastern side of the trench were siliceous mud mainly composed of diatoms,radiolarian,and sponge needles from surface to deep layer.The vertical profile of the elements,the concentration of TOC and the fossil record indicated that the sediment sample from station Dive113 was well mixed from surface to bottom layer.Based on the correlation of concentrations of the elements,the morphological characteristics of the sediment,and the ratios of Fe/Al and Ti/Al,we inferred that the sediment in the hadal zone of the trench had terrestrial,volcanic,biological,and authigenic sources.Major source of the sediment in the eastern side of the trench were terrestrial;whereas the sediment in the western side of the trench received more volcaniclastic input.
基金Supported by the National Basic Research Program of China(973 Program)(No.2015CB755904)the National Key Research and Development Program of China(No.2016YFA0601302)+1 种基金the National Natural Science Foundation of China(No.41676067)the Fundamental Funds for Central Universities(No.201762030)。
文摘In this study,cored sediment samples collected by the Jiaolong Submersible at 6779 m depth from the hadal zone of the Yap Trench in May 2016 were sliced in 1-cm interval from top to bottom,and lipids in each sediment layer were quantitatively and qualitatively analyzed.The vertical distribution profiles of the lipids in the sediment sample,their main existing forms,and their possible sources were investigated.The results show that the concentration of lipid in the surface sediment was the highest with the carbon number from 12 to 27,dominated by medium and short-chain lipids.The total concentration of fatty acids in surface sediment was much higher than those in the offshore and deep-sea areas,being up to 325.77μg/g due to the funnel effect caused by the“V”terrain of the trench.Fatty acid 18:0 was the most abundant lipids in the sediment sample.Abnormal high concentrations of fatty acid 18:1ω7 and alkanes indicated the existence of hydrothermal fluids in the study area.In addition,saturated fatty acids and monounsaturated fatty acids existed mainly in free form,and polyunsaturated fatty acids existed mainly in bound form.Most of the alkanes were in bound form,and their major source was autochthounous input.The carbon number of alcohols in the sediment sample ranged from 12 to 20,mainly existed in bound form.The source of fatty acids was mainly autochthonous input,and the neutral lipids had both marine and terrestrial origin.This is the first study of lipids in hadal sediment of the Yap Trench.The results will promote deeper understanding of organic carbon cycle in marine environment.
文摘背景:针对颈椎后路单开门椎管扩大成形术,C3椎板2种处理方式(C3椎板切除与C3椎板成形)的临床效果以及对术后颈椎功能的影响如何,相关客观评价较少,为此进行了此项研究。目的:系统评价颈椎后路椎管扩大成形术中C3椎板切除与C3椎板成形治疗多节段脊髓型颈椎病的疗效。方法:计算机检索Cochrane Central、PubMed、EMbase、the ISI Web of Knowledge Database、CNKI、CMB、维普、万方数据库,检索时间均为建库时间至2018-07-01。纳入关于C3椎板切除和椎板成形治疗多节段脊髓型颈椎病疗效对比的随机或非随机对照试验。Meta分析采用Cochrane协作网提供的Rev-Man5.3软件进行。结果与结论:①共纳入5个研究,316例患者,其中C3椎板切除组146例,C3椎板成形组170例;②Mate分析结果显示,在术后颈椎功能方面,与传统C3椎板成形相比,C3椎板切除术后颈椎活动度丢失较少[SMD=-5.89,95%CI(-7.25,-4.70),P <0.05]、术后轴性症状发生率低[SMD=-0.23,95%CI(0.12,0.46),P<0.05];③在神经功能恢复方面,2组术后日本骨科协会评分差异无显著性意义[SMD=-0.02,95%CI(-0.55,0.51),P=0.95> 0.05];④结果表明,采用椎管扩大成形治疗多节段脊髓型颈椎病时C3椎板的2种处理方式(椎板切除或椎板成形)均能取得较好的临床效果,但椎板切除能有效防止颈椎活动度丢失、降低术后轴性症状发生率。
