OBJECTIVE: To analyze part of the mechanism of electroacupuncture on Sishencong(EX-HN1) for strokerelated sleep disorders(SSD) and post-stroke cognitive impairment(PSCI). METHODS: Using a randomized controlled trial(R...OBJECTIVE: To analyze part of the mechanism of electroacupuncture on Sishencong(EX-HN1) for strokerelated sleep disorders(SSD) and post-stroke cognitive impairment(PSCI). METHODS: Using a randomized controlled trial(RCT) design, 72 patients were assigned to the electroacupuncture(EA) group or the sham acupuncture(SA) group. A healthy control(HC) group was also included. Both groups were given routine rehabilitation treatment. Then, patients in the EA group were given additional electroacupuncture at Sishencong(EX_HN1). Meanwhile, patients in the SA group were given a flat-head needle sham/placebo treatment placed at the bilateral Jianyu (LI15) and Binao(LI14) line midpoints and the Jianyu(LI15) and Jianzhen(SI9) line midpoints. Before and after treatment, scales were collected and analyzed. In the second phase of the study, some subjects from the EA group were selected for functional magnetic resonance imaging(f MRI) data acquisition and comparative analysis with the HC group using a non-RCT design. RESULTS: The EA group performed better than the SA group on the Pittsburgh sleep quality index(PSQI), Montreal cognitive assessment basic(Mo CA_B), selfrating anxiety scale(SAS), and self-rating depression scale(SDS). Analysis of the f MRI showed that lowfrequency(2 Hz) electroacupuncture stimulation at Sishencong(EX_HN1) can restrain frontal sup medial right(SFGmed.R), precuneus right(PCUN.R), and posterior cingulate cortex right(PCC.R) and enhance angular left(ANG.L), parietal inf left(IPL.L) and occipital mid left(MOG.L). The functional connectivity(FC) of SFGmed.R was positively correlated with PSQI. Electroacupuncture stimulation at Sishencong(EX_HN1) can reduce the side efficiency of the whole brain connection with the Thalamus.L, Hippocampus.L, and Occipital.Mid.L. CONCLUSIONS: Low frequency(2 Hz) electroacupuncture stimulation at Sishencong(EX_HN1) can simultaneously improve sleep quality, negative emotions, and cognitive functions, the first two of which may be related to SFGmed.R restraint. Electroacupuncture can make some brain areas approach the physiological bias state, which is characterized by dominant hemispheric enhancement and non-dominant hemispheric weakening. The reduced whole brain connection side efficiency with some key nodes of the brain net may relate to sleep quality improvements in SSD patients.展开更多
基金the National Natural Science Foundation of China Project:the Study of Central Mechanism of Electroacupuncture on Sishencong (EX-HN1) Improving Sleep Architecture and Neurocognitive Function Using Electroencephalogram-Functional Magnetic Resonance Imaging (No. 81774426)Major Scientific Research Project of Wuxi Health Commission:Clinical Study of Intelligent Hand Rehabilitation Training System for Nerve Function Reconstruction of Patients with Hand Dysfunction after Cerebral Infarction (No. Z202121)。
文摘OBJECTIVE: To analyze part of the mechanism of electroacupuncture on Sishencong(EX-HN1) for strokerelated sleep disorders(SSD) and post-stroke cognitive impairment(PSCI). METHODS: Using a randomized controlled trial(RCT) design, 72 patients were assigned to the electroacupuncture(EA) group or the sham acupuncture(SA) group. A healthy control(HC) group was also included. Both groups were given routine rehabilitation treatment. Then, patients in the EA group were given additional electroacupuncture at Sishencong(EX_HN1). Meanwhile, patients in the SA group were given a flat-head needle sham/placebo treatment placed at the bilateral Jianyu (LI15) and Binao(LI14) line midpoints and the Jianyu(LI15) and Jianzhen(SI9) line midpoints. Before and after treatment, scales were collected and analyzed. In the second phase of the study, some subjects from the EA group were selected for functional magnetic resonance imaging(f MRI) data acquisition and comparative analysis with the HC group using a non-RCT design. RESULTS: The EA group performed better than the SA group on the Pittsburgh sleep quality index(PSQI), Montreal cognitive assessment basic(Mo CA_B), selfrating anxiety scale(SAS), and self-rating depression scale(SDS). Analysis of the f MRI showed that lowfrequency(2 Hz) electroacupuncture stimulation at Sishencong(EX_HN1) can restrain frontal sup medial right(SFGmed.R), precuneus right(PCUN.R), and posterior cingulate cortex right(PCC.R) and enhance angular left(ANG.L), parietal inf left(IPL.L) and occipital mid left(MOG.L). The functional connectivity(FC) of SFGmed.R was positively correlated with PSQI. Electroacupuncture stimulation at Sishencong(EX_HN1) can reduce the side efficiency of the whole brain connection with the Thalamus.L, Hippocampus.L, and Occipital.Mid.L. CONCLUSIONS: Low frequency(2 Hz) electroacupuncture stimulation at Sishencong(EX_HN1) can simultaneously improve sleep quality, negative emotions, and cognitive functions, the first two of which may be related to SFGmed.R restraint. Electroacupuncture can make some brain areas approach the physiological bias state, which is characterized by dominant hemispheric enhancement and non-dominant hemispheric weakening. The reduced whole brain connection side efficiency with some key nodes of the brain net may relate to sleep quality improvements in SSD patients.
文摘目的:探究三氧化二砷(arsenic trioxide,As_2O_3)作用于结直肠癌的靶基因。方法:利用pharm GKB数据库和drugbank5. 0数据库分别提取三氧化二砷的直接靶基因,利用STRING10. 5数据库筛选与三氧化二砷已知靶基因互相作用的蛋白,利用web Gestalt数据库对互作蛋白进行通路分析和GO注释,运用c Bio Protal数据库和The Human Protein Atlas数据库对重点通路的核心基因进行变异和表达分析。结果:pharm GKB数据库和drugbank5. 0数据库共提取出11个直接靶基因,STRING10. 5数据库筛选出384个互作蛋白,web Gestalt数据库的GO注释这384个靶基因主要集中于19类分子功能,在前10条通路中筛选出3条与结直肠癌有关的通路,3个核心基因在结直肠癌中存在点突变和基因缺失,其中NF-κB1和RELA在结直肠癌组织中存在高表达。结论:三氧化二砷可能通过NF-κB信号通路治疗结直肠癌。
