Compared with the traditional contract management mode in the construction industry, the EPC project management mode has many advantages, such as simplifying the owner management, single contractor, clear responsibili...Compared with the traditional contract management mode in the construction industry, the EPC project management mode has many advantages, such as simplifying the owner management, single contractor, clear responsibilities, resource integration, improving quality and efficiency, etc. This paper uses SWOT analysis method to analyze the advantages, disadvantages, opportunities and threats of the EPC management model in the construction industry, to plan how to better promote the EPC management model, and to introduce the value and benefits of the EPC management model in terms of quality improvement, cost control, smart construction and green construction in combination with all the EPC projects completed in the past, so as to provide certain reference for the future development of EPC projects.展开更多
Epstein-Barr virus (EBV) encoded latent membrane protein 1 (LMP1) is considered as the major oncogenic protein of EBV encoded proteins, which could transactivate many transcription factors including activator protein ...Epstein-Barr virus (EBV) encoded latent membrane protein 1 (LMP1) is considered as the major oncogenic protein of EBV encoded proteins, which could transactivate many transcription factors including activator protein 1 (AP-1). Transcription factor plays its role in biological effects through binding to the target gene promoter,transactivating the transcription of target gene, regulating the target gene expression, etc. Recently, we found that LMP1 could mediate a new heterodimer form of c-Jun and JunB, which could bind to AP1 DNA sequence. In this report,we confirmed p16 as a putative target gene of c-Jun/JunB using bioinformatics. We used Tet-on-LMP1 HNE2 cell line as a cell model, which is a dual-stable LMP1 integrated HNE2 cell line and the expression of LMP1 could be regu-lated by the Tet system, and we wanted to explore whether c-Jun/JunB heterodimers mediated by LMP1 could regulate p16. Data demonstrated that c-Jun/Jun B heterodimers me-diated by LMP1 downregulated both the promoter activity and p16 expression, and accelerated the cell cycle progression. These findings established a new direct connection between the AP-I singnal pathway and the cell cycle, and provided a new model for the carcinogenesis mechanism.展开更多
文摘Compared with the traditional contract management mode in the construction industry, the EPC project management mode has many advantages, such as simplifying the owner management, single contractor, clear responsibilities, resource integration, improving quality and efficiency, etc. This paper uses SWOT analysis method to analyze the advantages, disadvantages, opportunities and threats of the EPC management model in the construction industry, to plan how to better promote the EPC management model, and to introduce the value and benefits of the EPC management model in terms of quality improvement, cost control, smart construction and green construction in combination with all the EPC projects completed in the past, so as to provide certain reference for the future development of EPC projects.
文摘Epstein-Barr virus (EBV) encoded latent membrane protein 1 (LMP1) is considered as the major oncogenic protein of EBV encoded proteins, which could transactivate many transcription factors including activator protein 1 (AP-1). Transcription factor plays its role in biological effects through binding to the target gene promoter,transactivating the transcription of target gene, regulating the target gene expression, etc. Recently, we found that LMP1 could mediate a new heterodimer form of c-Jun and JunB, which could bind to AP1 DNA sequence. In this report,we confirmed p16 as a putative target gene of c-Jun/JunB using bioinformatics. We used Tet-on-LMP1 HNE2 cell line as a cell model, which is a dual-stable LMP1 integrated HNE2 cell line and the expression of LMP1 could be regu-lated by the Tet system, and we wanted to explore whether c-Jun/JunB heterodimers mediated by LMP1 could regulate p16. Data demonstrated that c-Jun/Jun B heterodimers me-diated by LMP1 downregulated both the promoter activity and p16 expression, and accelerated the cell cycle progression. These findings established a new direct connection between the AP-I singnal pathway and the cell cycle, and provided a new model for the carcinogenesis mechanism.
