In this study, mitotic metaphase chromosomes in mouse were identified by a new chromosome fluorescence banding technique combining DAPI staining with image analysis. Clear 4＇, 6-diamidino-2-phenylindole （DAPI） mult...In this study, mitotic metaphase chromosomes in mouse were identified by a new chromosome fluorescence banding technique combining DAPI staining with image analysis. Clear 4＇, 6-diamidino-2-phenylindole （DAPI） multiple bands like （J-hands could be produced in mouse. The Meta- Morph software was then used to generate linescans of pixel intensity for the banded chromosomes from short arm to long arm. These linescans were sufficient not only to identify each individual chromosome but also analyze the physical sites of bands in chromosome. Based on the results, the clear and accurate karyotype of mouse metaphase chromosomes was established. The technique is therefore considered to he a new method for cytological studies of mouse.展开更多
The technique of simultaneous G banding and in situ hybridization has been developed in plants for the first time.Using this technique.RFLP marker umc58 closely linked with the hm1 gene dictating Helminthosporium carb...The technique of simultaneous G banding and in situ hybridization has been developed in plants for the first time.Using this technique.RFLP marker umc58 closely linked with the hm1 gene dictating Helminthosporium carbonum susceptibility1 was localized onto 1L3(chromosome 1,long arm,the third band from the centromere to the end of the arm),5L5 and 9L5.Theresults demonstrated that umc58 was a tripli cated sequence.It was deduced that umc58 probably was in a duplicated region that includes a part of Helminthosporium carbonum susceptibility genes(hm1 and hm2),as the hybridization sites of umc58 in chro mosomes 1 and 9 were those at which the genes localize.The techniques of simultaneous G banding and ISH in plants are discussed.展开更多
Ten terminal or subterminal RFLP markers belonging to linkage groups 1, 3, 5, 6, and 10 in maize RFLP map were physically located onto maize mitotic chromosomes with in situ hybridization. All biotinylated probes from...Ten terminal or subterminal RFLP markers belonging to linkage groups 1, 3, 5, 6, and 10 in maize RFLP map were physically located onto maize mitotic chromosomes with in situ hybridization. All biotinylated probes from 600 to 2 250 bp were detected by DAB staining. The markers belonging to linkage groups 1, 3, 5, 6, and 10 correspondingly located at the chromosomes 1, 3, 5, 6, and 10. All of the tested markers except bnl6.25 and umc44 were duplicated sequences. Each of them was also labeled on another chromosome besides on the chromosome corresponding to its linkage group. The marker bnl3.04 was triplicated sequences and the signals were detected on three nonhomologous chromosomes. In the tested ten markers, there were only four located at the ends of corresponding chromosomes. Others were located at sites midway along the chromosome arms or near the centromeres. The region covered by two terminal or subterminal markers in each of linkage groups 1, 3, 5, and 6 occupied 80.02%, 38.25%, 82.30% and 51.16% of the region of both short and long arms in chromosomes 1, 3, 5,and 6 respectively. Only two terminal markers of linkage group 10 covered the whole chromosome 10. In some linkage groups, two terminal or subterminal markers covered a short genetic distance but were physically distant, while two covering a longer genetic distance were physically closer.展开更多
A fluorescence in situ hybridization (FISH) procedure was adopted to physically map two rice BAC clones 24E21 and 4F22 linked to Gm-6 and Pi-5(t) in O. offi-cinalis. FISH results showed that the two BAC clones were lo...A fluorescence in situ hybridization (FISH) procedure was adopted to physically map two rice BAC clones 24E21 and 4F22 linked to Gm-6 and Pi-5(t) in O. offi-cinalis. FISH results showed that the two BAC clones were located at 4L. The percentage distance from the centromere to the hybridization sites was 72 ± 2.62 for 24E21 and 54± 5.43 for 4F22, the detection rates were 52.70% and 61.2%. The results obtained from the BAC and plasmid clones, RG214 and RZ565 of cultivated rice and O. officinalis were the same. This suggested that the markers, RG214 and RZ565 of cultivated rice and O. officinalis were in the same BAC clones. The homologous sequences of Gm-6 and Pi-5(t) in O. officinalis were positions that signals existed on the 4L. Many signals were observed when no Cot-1 DNA blocked. This also showed that repetitive sequences were some ho-molgous between cultivated rice and O. officinalis. The identification of chromosome 4 of O. officinalis is based on Jena et al. (1994). In our study, we discussed展开更多
Oryza granulata Nees et Arn.ex Watt.is one of the three wild relatives of rice,which are the most valuable for study and utilization in China.In this study,the homol-ogy and physical locations of three rice resistance...Oryza granulata Nees et Arn.ex Watt.is one of the three wild relatives of rice,which are the most valuable for study and utilization in China.In this study,the homol-ogy and physical locations of three rice resistance genes,Glh,Bph-3 and xa-5 are comparatively analyzed between O.sativa and O.granulata by Southern blotting and fluorescence in situ hybridization(FISH).The results of Southern blotting indicate that there exist homologous sequences of the tested RFLP markers in O.granulata.By using three bacterial arti-ficial chromosome(BAC)clones scanned by the tested RFLP as probes,FISH signals are detected on both mitotic and pachytene chromosomes in O.sativa and O.granulata.Dual-color FISH demonstrates that two of the three BAC clones(14E16 and 38J9)are located on the short arm of the same chromosome pair in O.granulata.Additionally,colin-earity is shown for the two clones between O.sativa and O.granulata.Another BAC clone 44B4 is located on the end of the short arm of other chromosome pair in these two species.Although the phylogenetic relationship between O.sativa and O.granulata is the most distinct in Oryza and these two spe-cies have evidently different biological features and ecologi-cal habits,the relative lengths and arm ratios of the detected chromosomes and the relative positions of the tested clone signals on chromosomes in O.granulata are quite similar to those in O.sativa.展开更多
Knob-associated tandem repeats,180-bp repeats and TR-1 elements,together with 45S rDNA were located on mitotic chromosomes of Zea diploperennis(DP),maize inbred line F102 and their hybrid.In DP,knobs on the short arm ...Knob-associated tandem repeats,180-bp repeats and TR-1 elements,together with 45S rDNA were located on mitotic chromosomes of Zea diploperennis(DP),maize inbred line F102 and their hybrid.In DP,knobs on the short arm of chromosomes 1 and 4 and on the long arm ofthe chromosomes 4 and 5 are composed predominantly of the 180-bp repeats.In addition,180-bp repeats existed together with TR-1 elements were also detected on the short arm ofchromosomes 2 and 5 and on the long arm of the chromosomes 2,6,7,8 and 9.In maize inbred line F102,180-bp repeats were present in chromosomes 7S and one homologue of chromosomes 8L.TR-1 elements appeared on satellite of chromosome 6 and no detectable hybridization site co-located with 180-bp repeats was observed in maize F102.Polymorphism of size,number,and distribution of 180-bp and TR-1 signals were revealed among different chromosomes in these two species and heteromorphism existed between some homologous chromosomes in the same species.Using these excellent landmarks,the interspecific hybrid of maize and DP were identified.The results suggest that comparative analysis of 180-bp repeats and TR-1 elements may help understand the genome organization and the evolution in Zea.展开更多
Using multi-color fluorescence in situ hybridization (FISH), we localized transferred barnase-psl and pHctinG DNA sequences onto chromosomes of two trans-genie rice plants, named Q12 and Q13, both of which were produc...Using multi-color fluorescence in situ hybridization (FISH), we localized transferred barnase-psl and pHctinG DNA sequences onto chromosomes of two trans-genie rice plants, named Q12 and Q13, both of which were produced by micro-projectile bombardment. In both Q12 and Q13, each detected cell showed 2-3 signal spots on their chromosomes respectively. The signals of both barnase-psl and pHctinG were mostly detected in the adjacent chromosomal sites in which their signals were overlapped and could be recognized by the signal color on the metaphase chromosomes. Fiber FISH further demonstrated that the multiple copies in each of the two DNA sequences distributed adjacently on the DNA fiber in Q13. Combined with the results of Southern hybridization, the possible integration patterns in transgenic rice co-transformed by micro-projectile bombardment have been discussed.展开更多
More and more studies demonstrate that a great deal of interactions among the quantitative trait loci (QTLs) are far more than those detected by single markers. A correlation method was proposed for estimating the int...More and more studies demonstrate that a great deal of interactions among the quantitative trait loci (QTLs) are far more than those detected by single markers. A correlation method was proposed for estimating the interactions of multiple QTLs detected by multi-markers in several mapping populations. Genetic implication of this method and usage were discussed.展开更多
文摘In this study, mitotic metaphase chromosomes in mouse were identified by a new chromosome fluorescence banding technique combining DAPI staining with image analysis. Clear 4＇, 6-diamidino-2-phenylindole （DAPI） multiple bands like （J-hands could be produced in mouse. The Meta- Morph software was then used to generate linescans of pixel intensity for the banded chromosomes from short arm to long arm. These linescans were sufficient not only to identify each individual chromosome but also analyze the physical sites of bands in chromosome. Based on the results, the clear and accurate karyotype of mouse metaphase chromosomes was established. The technique is therefore considered to he a new method for cytological studies of mouse.
文摘The technique of simultaneous G banding and in situ hybridization has been developed in plants for the first time.Using this technique.RFLP marker umc58 closely linked with the hm1 gene dictating Helminthosporium carbonum susceptibility1 was localized onto 1L3(chromosome 1,long arm,the third band from the centromere to the end of the arm),5L5 and 9L5.Theresults demonstrated that umc58 was a tripli cated sequence.It was deduced that umc58 probably was in a duplicated region that includes a part of Helminthosporium carbonum susceptibility genes(hm1 and hm2),as the hybridization sites of umc58 in chro mosomes 1 and 9 were those at which the genes localize.The techniques of simultaneous G banding and ISH in plants are discussed.
文摘Ten terminal or subterminal RFLP markers belonging to linkage groups 1, 3, 5, 6, and 10 in maize RFLP map were physically located onto maize mitotic chromosomes with in situ hybridization. All biotinylated probes from 600 to 2 250 bp were detected by DAB staining. The markers belonging to linkage groups 1, 3, 5, 6, and 10 correspondingly located at the chromosomes 1, 3, 5, 6, and 10. All of the tested markers except bnl6.25 and umc44 were duplicated sequences. Each of them was also labeled on another chromosome besides on the chromosome corresponding to its linkage group. The marker bnl3.04 was triplicated sequences and the signals were detected on three nonhomologous chromosomes. In the tested ten markers, there were only four located at the ends of corresponding chromosomes. Others were located at sites midway along the chromosome arms or near the centromeres. The region covered by two terminal or subterminal markers in each of linkage groups 1, 3, 5, and 6 occupied 80.02%, 38.25%, 82.30% and 51.16% of the region of both short and long arms in chromosomes 1, 3, 5,and 6 respectively. Only two terminal markers of linkage group 10 covered the whole chromosome 10. In some linkage groups, two terminal or subterminal markers covered a short genetic distance but were physically distant, while two covering a longer genetic distance were physically closer.
基金the National Natural Science Foundation ofChina (Grant No. 39870423) and the Doctorate Vesting Point Foundation of the State Education Commission of China (Grant No. 207980112).
文摘A fluorescence in situ hybridization (FISH) procedure was adopted to physically map two rice BAC clones 24E21 and 4F22 linked to Gm-6 and Pi-5(t) in O. offi-cinalis. FISH results showed that the two BAC clones were located at 4L. The percentage distance from the centromere to the hybridization sites was 72 ± 2.62 for 24E21 and 54± 5.43 for 4F22, the detection rates were 52.70% and 61.2%. The results obtained from the BAC and plasmid clones, RG214 and RZ565 of cultivated rice and O. officinalis were the same. This suggested that the markers, RG214 and RZ565 of cultivated rice and O. officinalis were in the same BAC clones. The homologous sequences of Gm-6 and Pi-5(t) in O. officinalis were positions that signals existed on the 4L. Many signals were observed when no Cot-1 DNA blocked. This also showed that repetitive sequences were some ho-molgous between cultivated rice and O. officinalis. The identification of chromosome 4 of O. officinalis is based on Jena et al. (1994). In our study, we discussed
基金supported by the National Natural Science Foundation of China(Grant No.39870423).
文摘Oryza granulata Nees et Arn.ex Watt.is one of the three wild relatives of rice,which are the most valuable for study and utilization in China.In this study,the homol-ogy and physical locations of three rice resistance genes,Glh,Bph-3 and xa-5 are comparatively analyzed between O.sativa and O.granulata by Southern blotting and fluorescence in situ hybridization(FISH).The results of Southern blotting indicate that there exist homologous sequences of the tested RFLP markers in O.granulata.By using three bacterial arti-ficial chromosome(BAC)clones scanned by the tested RFLP as probes,FISH signals are detected on both mitotic and pachytene chromosomes in O.sativa and O.granulata.Dual-color FISH demonstrates that two of the three BAC clones(14E16 and 38J9)are located on the short arm of the same chromosome pair in O.granulata.Additionally,colin-earity is shown for the two clones between O.sativa and O.granulata.Another BAC clone 44B4 is located on the end of the short arm of other chromosome pair in these two species.Although the phylogenetic relationship between O.sativa and O.granulata is the most distinct in Oryza and these two spe-cies have evidently different biological features and ecologi-cal habits,the relative lengths and arm ratios of the detected chromosomes and the relative positions of the tested clone signals on chromosomes in O.granulata are quite similar to those in O.sativa.
基金was supported by the National Natural Science Foundation of China(Grant No.39870423).
文摘Knob-associated tandem repeats,180-bp repeats and TR-1 elements,together with 45S rDNA were located on mitotic chromosomes of Zea diploperennis(DP),maize inbred line F102 and their hybrid.In DP,knobs on the short arm of chromosomes 1 and 4 and on the long arm ofthe chromosomes 4 and 5 are composed predominantly of the 180-bp repeats.In addition,180-bp repeats existed together with TR-1 elements were also detected on the short arm ofchromosomes 2 and 5 and on the long arm of the chromosomes 2,6,7,8 and 9.In maize inbred line F102,180-bp repeats were present in chromosomes 7S and one homologue of chromosomes 8L.TR-1 elements appeared on satellite of chromosome 6 and no detectable hybridization site co-located with 180-bp repeats was observed in maize F102.Polymorphism of size,number,and distribution of 180-bp and TR-1 signals were revealed among different chromosomes in these two species and heteromorphism existed between some homologous chromosomes in the same species.Using these excellent landmarks,the interspecific hybrid of maize and DP were identified.The results suggest that comparative analysis of 180-bp repeats and TR-1 elements may help understand the genome organization and the evolution in Zea.
基金This work was supported by the National Natural Science Foundation of China (Grant No. 39900083) the Research Fund for the Doctoral Program of Higher Education (Grant No. 207980112).
文摘Using multi-color fluorescence in situ hybridization (FISH), we localized transferred barnase-psl and pHctinG DNA sequences onto chromosomes of two trans-genie rice plants, named Q12 and Q13, both of which were produced by micro-projectile bombardment. In both Q12 and Q13, each detected cell showed 2-3 signal spots on their chromosomes respectively. The signals of both barnase-psl and pHctinG were mostly detected in the adjacent chromosomal sites in which their signals were overlapped and could be recognized by the signal color on the metaphase chromosomes. Fiber FISH further demonstrated that the multiple copies in each of the two DNA sequences distributed adjacently on the DNA fiber in Q13. Combined with the results of Southern hybridization, the possible integration patterns in transgenic rice co-transformed by micro-projectile bombardment have been discussed.
基金This research was supported by the National Natural Science Foundation of China (Grant No. 30070425).
文摘More and more studies demonstrate that a great deal of interactions among the quantitative trait loci (QTLs) are far more than those detected by single markers. A correlation method was proposed for estimating the interactions of multiple QTLs detected by multi-markers in several mapping populations. Genetic implication of this method and usage were discussed.
