The herbal plant Acanthopanax Senticosus Harms is natural herb of Changbaishan in Jilin Province of China, which belongs to the Araliaceae family. As the ingredients of folk medicine, it has long been used to treat a ...The herbal plant Acanthopanax Senticosus Harms is natural herb of Changbaishan in Jilin Province of China, which belongs to the Araliaceae family. As the ingredients of folk medicine, it has long been used to treat a variety of human diseases, such as cerebrovascular disease, diabetes, tumor, isochemic heart diseases, hypertension, rheumatic arthritis, etc. E2,33. Flavonoids, a class of constituent compounds, which have a broad distribution in the nature and are found in Acanthopanax Senticosus Harms, have gained particular attention. A number of studies have shown that flavonoid compounds have wide biological activities, such as anti-aging, anticancer, anti-HIV,展开更多
A high performance liquid chromatography coupled with electrospray ionization-tandem mass spectrome try(HPLC-ESI-MS/MS)method was developed for the analysis and identification of ginsenosides in the extracts of raw Pa...A high performance liquid chromatography coupled with electrospray ionization-tandem mass spectrome try(HPLC-ESI-MS/MS)method was developed for the analysis and identification of ginsenosides in the extracts of raw Panax ginseng(RPG)and steamed Panax ginseng at high temperatures(SPGHT).A total of 25 ginsenosides were extracted include of which 10 low-polar ginsenosides,such as ginsenosides F4,Rk3,Rh4,20S-Rg3,20R-Rg3 and so on,were identified according to their HPLC retention time and MS/MS data.The results indicated that the low polar ginsenosides were seldom found in RPG.For the exploration of the transformation pattern of the ginsenosides in steam processing,the standards of ginsenosides Re,Rg1,Rb1,Rc,Rb2,Rb3 and Rd were selected and hydrolyzed at a temperature of 120 oC.The results show that these polar ginsenosides can be converted to low-polar ginsenosides such as Rg2,Rg6,F4,Rk3 and Rg5 by hydrolyzing the sugar chains.展开更多
Radix Scutellaria is widely applied to the treatment of diabetes mellitus in China. Its main bioactive constituents contain baicalin, wogonoside, oroxyloside, and their aglycones. To investigate the effect of type 2 d...Radix Scutellaria is widely applied to the treatment of diabetes mellitus in China. Its main bioactive constituents contain baicalin, wogonoside, oroxyloside, and their aglycones. To investigate the effect of type 2 diabetes mellitus on both pharmacokinetics and tissue distribution of these flavonoid compounds, the six flavonoids in plasma and tissues from the normal and type 2 diabetic rats after oral administration of Radix Scutellaria extract were simultaneously measured by ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) method. The results showed that baicalin, wogonoside, and oroxyloside had higher Cmax and AUC values(P < 0.05) in type 2 diabetic rats than that in normal rats and the tissue-distribution behaviors of the six flavonoid compounds in hearts, livers, spleens, lungs, kidneys, brains, pancreas, fat and muscle of the type 2 diabetic rats showed obviously differences from the normal rats(P < 0.05). In conclusion, the differences in the pharmacokinetics of oroxyloside and tissue distribution of the six flavanoids in Radix Scutellaria extract between diabetic and normal rats were found for the first time. The results from the present study provided a crucial basis for a better understanding of in vivo anti-diabetic mechanism of action of the six flavonoids from Radix Scutellaria.展开更多
The near-infrared（NIR） diffuse reflectance spectroscopy was used to study the content of Berberine in the processed Coptis. The allocated proportions of Coptis to ginger, yellow liquor or Evodia rutaecarpa changed a...The near-infrared（NIR） diffuse reflectance spectroscopy was used to study the content of Berberine in the processed Coptis. The allocated proportions of Coptis to ginger, yellow liquor or Evodia rutaecarpa changed according to the results of orthogonal design as well as the temperature. For as withdrawing the full and effective information from the spectral data as possible, the spectral data was preprocessed through first derivative and multiplicative scatter correetion（MSC） according to the optimization results of different preprocessing methods. Firstly, the model was established by partial least squares（PLS）; the coefficient of determination（R2） of the prediction was 0.839, the root mean squared error of prediction（RMSEP） was 0.1422, and the mean relative error（RME） was 0.0276. Secondly, for reducing the dimension and removing noise, the spectral variables were highly effectively compressed via the wavelet transformation（WT） technology and the Haar wavelet was selected to decompose the spectral signals. After the wavelet coefficients from WT were input into the artificial neural network（ANN） instead of the spectra signal, the quantitative analysis model of Berberine in processed Coptis was established. The R^2 of the model was 0.9153, the RMSEP was 0.0444, and the RME was 0.0091. The values of appraisal index, namely R^2, RMSECV, and RME, indicate that the generalization ability and prediction precision of ANN are superior to those of PLS. The overall results show that NIR spectroscopy combined with ANN can be efficiently utilized for the rapid and accurate analysis of routine chemical compositions in Coptis. Accordingly, the result can provide technical support for the further analysis of Berberine and other components in processed Coptis. Simultaneously, the research can also offer the foundation of quantitative analysis of other NIR application.展开更多
In vitro models of human colon carcinoma cell line(Caco-2 cell monolayer) and human intestinal bacteria were used to investigate the intestinal transport and biotransformation of resibufogenin and cinobufagin in Chan ...In vitro models of human colon carcinoma cell line(Caco-2 cell monolayer) and human intestinal bacteria were used to investigate the intestinal transport and biotransformation of resibufogenin and cinobufagin in Chan Su by HPLC/APCI-MSn. The experimental results of Caco-2 cell monolayer demonstrate that the apparent permeability coefficients(Papp) of resibufogenin and cinobufagin are higher than 10–6 cm/s, which indicates that both resibufogenin and cinobufagin have a good absorption in the small intestine. And the biotransformation result of human intestinal bacteria shows that resibufogenin has been transformed to 3-epiresibufogenin and cinobufagin has been transformed to 3-epicinobufagin, deacetylcinobufagin and 3-epideacetycinobufagin, respectively.展开更多
A practical solution of qualitatively analyzing quaternary alkaloids in coptis-scute herb couple by electrospray ionization mass spectrometry（ES1-MS） was developed. Without the complicated pretreatment of sample, th...A practical solution of qualitatively analyzing quaternary alkaloids in coptis-scute herb couple by electrospray ionization mass spectrometry（ES1-MS） was developed. Without the complicated pretreatment of sample, thc active ingredients including berberine, palmatine, coptisine, jatrorrhizine, epiberberine, and columbamine were identified and some relative content changing rules of alkaloids in coptis-scute couple were summarized in this article. The overall profiles of the complex extracts were obtained. After adding an internal standard（rutaecarpine）, semi-quantitative analysis was performed and the result indicates that the actual content of alkaloids was decreased by increasing the amount of scute. Based on the data obtained by high-performance capillary electrophoresis（HPCE）, the feasibility of semi-quantitative analysis by ES1-MS was further proved.展开更多
A simple method was developed for rapid and direct profiling of alkaloids in medical herbs via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF MS）. The dry herbs were first gro...A simple method was developed for rapid and direct profiling of alkaloids in medical herbs via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF MS）. The dry herbs were first ground to powder and passed through a stainless steel sieve, mixed with the matrix solution to form a homogeneous suspension, which was then directly applied to MALDI analysis. Several matrices were investigated and 2,5- dihydroxybenzoic acid（DHB） was chosen as the optimized one, and the particle with small size was found to favor the analysis. Using this method, the profiles of alkaloids in several medical herbs were readily obtained, and the toxicities of crude and processed Radix Aconiti Lateralis Preparata were compared via the relative intensities of the peaks of the corresponding toxic components shown in their MALDI spectra. This method therefore provides a rapid and reliable protocol for obtaining profiles of alkaloids in medical herbs by using MALDI-TOF MS.展开更多
Introduction Aconitine-type alkaloids isolated from the roots of Aconitum carmiechaeli show a potential toxicity and a broad spectrum of bioactivity[1-4].On the basis of the C8-substituent of C19-diterpenoid skeleton,...Introduction Aconitine-type alkaloids isolated from the roots of Aconitum carmiechaeli show a potential toxicity and a broad spectrum of bioactivity[1-4].On the basis of the C8-substituent of C19-diterpenoid skeleton,aconitine-type alkaloids can be divided into diester-diterpenoid alkaloids（DDAs）,monoester-diterpenoid alkaloids（MDAs）,and lipo-alkaloids（Fig.1）.展开更多
Radiation causes severe constraint on numerous pathological functions of cells, such as cell growth, nuclear genetic material expression and cell functions. In this study, we performed proteomic profiling of a nuclear...Radiation causes severe constraint on numerous pathological functions of cells, such as cell growth, nuclear genetic material expression and cell functions. In this study, we performed proteomic profiling of a nuclear fraction of Jurkat T lymphocyte cells under radiation along different time course by means of 2DE and MALDI TOF-MS. We found 24 protein spots whose expression had changed after radiation, including relevant proteins, genetic material proteins, metabolism proteins, molecular chaperon and nuclear membrane proteins. Based on the above it is concluded that the combination of fluorescence labeled 2D-PAGE and MALDI-TOF MS is more precisely and effectively to elucidate the protein changes in Jurkat T lymphocyte cells after irradiation.展开更多
基金the Natural Science and Technology Foundation of Jilin Province(No 20020637-1)
文摘The herbal plant Acanthopanax Senticosus Harms is natural herb of Changbaishan in Jilin Province of China, which belongs to the Araliaceae family. As the ingredients of folk medicine, it has long been used to treat a variety of human diseases, such as cerebrovascular disease, diabetes, tumor, isochemic heart diseases, hypertension, rheumatic arthritis, etc. E2,33. Flavonoids, a class of constituent compounds, which have a broad distribution in the nature and are found in Acanthopanax Senticosus Harms, have gained particular attention. A number of studies have shown that flavonoid compounds have wide biological activities, such as anti-aging, anticancer, anti-HIV,
基金Supported by the Key Project of Jilin Provincial Science and Technology Department,China(No.20090908)the Project of Changchun Science and Technology Bureau,China(No.2008256)the Special Cooperative Project for Hitech Industrializa-tion of Jilin Provincial Science and Chinese Academy of Sciences,China(No.2009SYHZ0026)
文摘A high performance liquid chromatography coupled with electrospray ionization-tandem mass spectrome try(HPLC-ESI-MS/MS)method was developed for the analysis and identification of ginsenosides in the extracts of raw Panax ginseng(RPG)and steamed Panax ginseng at high temperatures(SPGHT).A total of 25 ginsenosides were extracted include of which 10 low-polar ginsenosides,such as ginsenosides F4,Rk3,Rh4,20S-Rg3,20R-Rg3 and so on,were identified according to their HPLC retention time and MS/MS data.The results indicated that the low polar ginsenosides were seldom found in RPG.For the exploration of the transformation pattern of the ginsenosides in steam processing,the standards of ginsenosides Re,Rg1,Rb1,Rc,Rb2,Rb3 and Rd were selected and hydrolyzed at a temperature of 120 oC.The results show that these polar ginsenosides can be converted to low-polar ginsenosides such as Rg2,Rg6,F4,Rk3 and Rg5 by hydrolyzing the sugar chains.
基金supported by the National Natural Science Foundation of China(Nos.81473537 and 81373952)
文摘Radix Scutellaria is widely applied to the treatment of diabetes mellitus in China. Its main bioactive constituents contain baicalin, wogonoside, oroxyloside, and their aglycones. To investigate the effect of type 2 diabetes mellitus on both pharmacokinetics and tissue distribution of these flavonoid compounds, the six flavonoids in plasma and tissues from the normal and type 2 diabetic rats after oral administration of Radix Scutellaria extract were simultaneously measured by ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) method. The results showed that baicalin, wogonoside, and oroxyloside had higher Cmax and AUC values(P < 0.05) in type 2 diabetic rats than that in normal rats and the tissue-distribution behaviors of the six flavonoid compounds in hearts, livers, spleens, lungs, kidneys, brains, pancreas, fat and muscle of the type 2 diabetic rats showed obviously differences from the normal rats(P < 0.05). In conclusion, the differences in the pharmacokinetics of oroxyloside and tissue distribution of the six flavanoids in Radix Scutellaria extract between diabetic and normal rats were found for the first time. The results from the present study provided a crucial basis for a better understanding of in vivo anti-diabetic mechanism of action of the six flavonoids from Radix Scutellaria.
基金Supported by the National Natural Science Foundation of China(No.50635030)the Key Project of Jilin Provincial De-partment of Science & Technology, China(Nos.20060902-02, 200705C07)
文摘The near-infrared（NIR） diffuse reflectance spectroscopy was used to study the content of Berberine in the processed Coptis. The allocated proportions of Coptis to ginger, yellow liquor or Evodia rutaecarpa changed according to the results of orthogonal design as well as the temperature. For as withdrawing the full and effective information from the spectral data as possible, the spectral data was preprocessed through first derivative and multiplicative scatter correetion（MSC） according to the optimization results of different preprocessing methods. Firstly, the model was established by partial least squares（PLS）; the coefficient of determination（R2） of the prediction was 0.839, the root mean squared error of prediction（RMSEP） was 0.1422, and the mean relative error（RME） was 0.0276. Secondly, for reducing the dimension and removing noise, the spectral variables were highly effectively compressed via the wavelet transformation（WT） technology and the Haar wavelet was selected to decompose the spectral signals. After the wavelet coefficients from WT were input into the artificial neural network（ANN） instead of the spectra signal, the quantitative analysis model of Berberine in processed Coptis was established. The R^2 of the model was 0.9153, the RMSEP was 0.0444, and the RME was 0.0091. The values of appraisal index, namely R^2, RMSECV, and RME, indicate that the generalization ability and prediction precision of ANN are superior to those of PLS. The overall results show that NIR spectroscopy combined with ANN can be efficiently utilized for the rapid and accurate analysis of routine chemical compositions in Coptis. Accordingly, the result can provide technical support for the further analysis of Berberine and other components in processed Coptis. Simultaneously, the research can also offer the foundation of quantitative analysis of other NIR application.
基金Supported by the National Natural Science Foundation of China(Nos.30772721 and 30873360)
文摘In vitro models of human colon carcinoma cell line(Caco-2 cell monolayer) and human intestinal bacteria were used to investigate the intestinal transport and biotransformation of resibufogenin and cinobufagin in Chan Su by HPLC/APCI-MSn. The experimental results of Caco-2 cell monolayer demonstrate that the apparent permeability coefficients(Papp) of resibufogenin and cinobufagin are higher than 10–6 cm/s, which indicates that both resibufogenin and cinobufagin have a good absorption in the small intestine. And the biotransformation result of human intestinal bacteria shows that resibufogenin has been transformed to 3-epiresibufogenin and cinobufagin has been transformed to 3-epicinobufagin, deacetylcinobufagin and 3-epideacetycinobufagin, respectively.
基金Supported by the National Basic Research Program of China(No.2006CB504706)the National Natural Science Foundation of China(No.30672600)+1 种基金the Great Research Project of Chinese Academy of Sciences(No.KGCX2-SW-213-06)the Key Project of Jilin Provinical Science and Technology, China(No.20060902)
文摘A practical solution of qualitatively analyzing quaternary alkaloids in coptis-scute herb couple by electrospray ionization mass spectrometry（ES1-MS） was developed. Without the complicated pretreatment of sample, thc active ingredients including berberine, palmatine, coptisine, jatrorrhizine, epiberberine, and columbamine were identified and some relative content changing rules of alkaloids in coptis-scute couple were summarized in this article. The overall profiles of the complex extracts were obtained. After adding an internal standard（rutaecarpine）, semi-quantitative analysis was performed and the result indicates that the actual content of alkaloids was decreased by increasing the amount of scute. Based on the data obtained by high-performance capillary electrophoresis（HPCE）, the feasibility of semi-quantitative analysis by ES1-MS was further proved.
基金Supported by the Major State Basic Research Development Program of China(No.2006CB5047060)the National Natural Science Foundation of China(Nos.30672600, 30772721)the Natural Science Foundation of Jilin Province, China (No.20060902)
文摘A simple method was developed for rapid and direct profiling of alkaloids in medical herbs via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF MS）. The dry herbs were first ground to powder and passed through a stainless steel sieve, mixed with the matrix solution to form a homogeneous suspension, which was then directly applied to MALDI analysis. Several matrices were investigated and 2,5- dihydroxybenzoic acid（DHB） was chosen as the optimized one, and the particle with small size was found to favor the analysis. Using this method, the profiles of alkaloids in several medical herbs were readily obtained, and the toxicities of crude and processed Radix Aconiti Lateralis Preparata were compared via the relative intensities of the peaks of the corresponding toxic components shown in their MALDI spectra. This method therefore provides a rapid and reliable protocol for obtaining profiles of alkaloids in medical herbs by using MALDI-TOF MS.
基金Supported by the National Natural Science Foundation of China(Nos.30472134and30672600)the National Basic Re-search Program of China(No.2006CB5047060).
文摘Introduction Aconitine-type alkaloids isolated from the roots of Aconitum carmiechaeli show a potential toxicity and a broad spectrum of bioactivity[1-4].On the basis of the C8-substituent of C19-diterpenoid skeleton,aconitine-type alkaloids can be divided into diester-diterpenoid alkaloids（DDAs）,monoester-diterpenoid alkaloids（MDAs）,and lipo-alkaloids（Fig.1）.
基金Supported by the National Natural Science Foundation of China(Nos.20675079, 20873137 and 30672600)
文摘Radiation causes severe constraint on numerous pathological functions of cells, such as cell growth, nuclear genetic material expression and cell functions. In this study, we performed proteomic profiling of a nuclear fraction of Jurkat T lymphocyte cells under radiation along different time course by means of 2DE and MALDI TOF-MS. We found 24 protein spots whose expression had changed after radiation, including relevant proteins, genetic material proteins, metabolism proteins, molecular chaperon and nuclear membrane proteins. Based on the above it is concluded that the combination of fluorescence labeled 2D-PAGE and MALDI-TOF MS is more precisely and effectively to elucidate the protein changes in Jurkat T lymphocyte cells after irradiation.
