The main raw material utilized in wood adhesives comes from petrochemical extractives.However,due to the excessive dependence on petrochemical resources and the adverse impact on the ecosystem and human wellbeing,ther...The main raw material utilized in wood adhesives comes from petrochemical extractives.However,due to the excessive dependence on petrochemical resources and the adverse impact on the ecosystem and human wellbeing,there is an increasing trend to develop byproduct protein-based adhesives in the current global food safety context.In this research,flaxseed meal was subjected to pretreatment,and trimethylolpropane triglycidyl ether(TTE)and ethylenediamine(EN)were utilized as crosslinkers to establish a more compact adhesive layer and to prevent water intrusion.The pretreatment decreased the FM/UB viscosity by 60%compared to FM.The combination of CD analysis indicated that the Urea-NaOH pretreatment effectively stretched the flaxseed meal protein.According to Fourier transform infrared(FTIR)spectroscopy,X-ray diffraction(XRD),and differential scanning calorimetry(DSC)were used to analyze the resulting adhesive’s reaction mechanism and thermal response.Furthermore,the physical properties of the adhesive were characterized using wet shear strength testing and SEM observation.Remarkably,the dry bond strength increased from 0.72 to 2.12 MPa,representing a 194.4%increase.The wet bonding strength of the adhesive was improved from 0.22 to 1.21 MPa,representing a 550%increase compared to the original flaxseed protein-based adhesive,which far exceeded the minimum requirement for plywood of Type II(≥0.7 MPa,by GB/T 9846-2015).This study demonstrated an eco-friendly and sustainable method for the development of protein adhesives as viable substitutes for petrochemical resins.展开更多
Backgrounds and Objective Angiopoietin-2(Ang-2)is a promising biomarker and therapeutic target for gastrointestinal angiodysplasia(GIAD).We hypothesized that the lncRNA-HIF1A-AS2/miR-153-3p/HIF-1α/Ang-2 axis plays a ...Backgrounds and Objective Angiopoietin-2(Ang-2)is a promising biomarker and therapeutic target for gastrointestinal angiodysplasia(GIAD).We hypothesized that the lncRNA-HIF1A-AS2/miR-153-3p/HIF-1α/Ang-2 axis plays a critical role in small bowel angiodysplasia(SBAD)-associated angiogenesis,which can be blocked by rifaximin.The purpose of this study was to investigate the expression and pro-angiogenic effects of the lncRNA-HIF1A-AS2/miR-153-3p/HIF-1α/Ang-2 in SBAD and to evaluate the therapeutic potential of rifaximin on SBAD by targeting this axis.Methods The expression and pro-angiogenic effects of lncRNA-HIF1A-AS2/miR-153-3p/HIF-1α/Ang-2 were analysed in SBAD tissues and human umbilical vein endothelial cells(HUVECs).The anti-angiogenic effect of rifaximin and its impact on the lncRNA-HIF1A-AS2/miR-153-3p/HIF-1α/Ang-2 axis were evaluated in HUVECs.Results Increased expression of lncRNA-HIF1A-AS2 and decreased expression of miR-153-3p were detected in SBAD tissues.LncRNA-HIF1A-AS2/miR-153-3p/HIF-1αwere upstream regulators of Ang-2,and this axis was involved in angiogenesis in HUVECs.Rifaximin exerted antiangiogenic effects on HUVECs by blocking this axis.Conclusions The lncRNA-HIF1A-AS2/miR-153-3p/HIF-1α/Ang-2 axis is critically involved in SBAD-associated angiogenesis.Rifaximin is a potential therapeutic option for SBAD via blockade of this axis.展开更多
采用电化学方法研究了深冷处理温度对AZ31镁合金CMT(cold metal transfer)焊接接头腐蚀行为的影响。结果表明:与未深冷时相比,经–100℃,4h;–140℃,4h;–180℃,4h 3组参数深冷处理后的接头焊缝区耐蚀性能得到不同程度提高,随着深冷温...采用电化学方法研究了深冷处理温度对AZ31镁合金CMT(cold metal transfer)焊接接头腐蚀行为的影响。结果表明:与未深冷时相比,经–100℃,4h;–140℃,4h;–180℃,4h 3组参数深冷处理后的接头焊缝区耐蚀性能得到不同程度提高,随着深冷温度降低,耐蚀性能呈先升高后下降的变化趋势,深冷处理对焊缝区中第二相的尺寸、含量及分布的改变是决定这一趋势的关键因素。其中经–140℃,4h深冷处理的焊缝区电荷转移电阻和腐蚀产物膜电阻最大,腐蚀电流最低,耐蚀性能最佳。展开更多
Corallorhiza sinensis,a new species of mycoheterotrophic orchid from western Sichuan,China,is described and illustrated based on molecular and morphological evidence.It is morphologically similar to Corallorhiza trifi...Corallorhiza sinensis,a new species of mycoheterotrophic orchid from western Sichuan,China,is described and illustrated based on molecular and morphological evidence.It is morphologically similar to Corallorhiza trifida,but can be distinguished by bigger flowers,both sepals and petals with 3 veins,and longer lateral lobes of lip.To distinguish the new Corallorhiza species and explore its phylogenetic position within subtribe Calypsoinae,this study employed sequences of the nuclear ribosomal DNA(nrDNA)and whole plastome assembled from the genome skimming approach.The plastome is 148,124 bp in length,including a pair of inverted repeats(IRs)of 26,165 bp,a large single-copy region(LSC)of 82,207 bp,and a small single-copy region(SSC)of 13,587 bp.Further,phylogenetic analyses were performed using nrDNA sequence and 79 coding sequences(CDSs)from 26 complete plastomes of subtribe Calypsoinae.The phylogenetic tree based on nrDNA sequence suggested that Corallorhiza is a monophyletic group,and strongly support C.sinensis as sister to the rest species of Corallorhiza.The plastid tree showed that 10 Corallorhiza species grouped into two clades and C.sinensis is most closely related to the North American C.striata and C.bentleyi instead of Oreorchis foliosa and O.angustata in the same clade.The plastid tree and nrDNA tree indicate Oreorchis is a paraphyletic.Although the topological conflicts are displayed between plastome and nrDNA phylogenies of C.sinensis,it is still the most closely related to Corallorhiza.Comparative analysis showed that C.sinensis populations are characteristic of the intermediate morphological traits between Corallorhiza and Oreorchis.The finding of this new species from China shed new light on the phylogeny of Oreorchis and Corallorhiza.展开更多
Complex-shaped optical lenses are of great interest in the areas of laser processing,machine vision,and optical communications.Traditionally,the processing of complex optical lenses is usually achieved by precision ma...Complex-shaped optical lenses are of great interest in the areas of laser processing,machine vision,and optical communications.Traditionally,the processing of complex optical lenses is usually achieved by precision machining combined with post-grinding or polishing,which is expensive,labor-intensive and difficult in the processing of ultra-complex optical lenses.Additive manufacturing is an emerging technology that provides significant advantages in producing highly intricate optical devices.However,the layer-by-layer method employed in such manufacturing processes has resulted in low printing speeds,as well as limitations in surface quality.To address these challenges,we apply tomographic volumetric printing(TVP)in this work,which can realize the integrated printing of complex structural models without layering.By coordinating the TVP and the meniscus equilibrium post-curing methods,ultra-fast fabrication of complex-shaped lenses with sub-nanometric roughness has been achieved.A2.5 mm high,outer diameter 9 mm spherical lens with a roughness value of RMS=0.3340 nm is printed at a speed of 3.1×10^(4)mm^(3)h^(-1).As a further demonstration,a complex-shaped fly-eye lens is fabricated without any part assembly.The designed spherical lens is mounted on a smartphone’s camera,and the precise alignments above the circuit board are captured.Upon further optimization,this new technology demonstrates the potential for rapid fabrication of ultra-smooth complex optical devices or systems.展开更多
Objective:To analyse the rar gene repertoire and characterise the rhondroitin sulphate A (CSA)-binding activity of the Duffy-binding like(I)BI.) domains encoded by the var2csa gene of a Plasmodium falciparum(P.falcipa...Objective:To analyse the rar gene repertoire and characterise the rhondroitin sulphate A (CSA)-binding activity of the Duffy-binding like(I)BI.) domains encoded by the var2csa gene of a Plasmodium falciparum(P.falciparum) isolate in Hainan Province,China.Methods:The sequences of var DBL1 regions were PCR-amplified,sequenced and the sequence characteristics was bioinformalically analysed.Recombinant proteins encoded by the var2csa genes were expressed and purified.The binding activities of the recombinant proteins to CSA receptor was detected by ELISA assays.Results:Fifty six unique DBI.a sequences were obtained,and the sequences represented similar diversity to the var genes of the genome parasite 3D7.There are two var2csa genes in the P.falciparum isolated from Hainan Province.Unlike in other falciparum parasites such as HB3,the two var2csa genes are more diverged.The receptor-binding capacity of DBL-5εand DBI.-6 e domains of HN var2CSA was studied.Conclusions:This work represented the diversity of rar genes of a P.falciparum isolate in China.展开更多
Combining the first-principles calculations and structural enumeration with recognition,the delithiation process of LiNiO_(2)is investigated,where various supercell shapes are considered in order to obtain the formati...Combining the first-principles calculations and structural enumeration with recognition,the delithiation process of LiNiO_(2)is investigated,where various supercell shapes are considered in order to obtain the formation energy of Li_(x)NiO_(2).Meanwhile,the voltage profile is simulated and the ordered phases of lithium vacancies corresponding to concentrations of 1/4,2/5,3/7,1/2,2/3,3/4,5/6,and 6/7 are predicted.To understand the capacity decay in the experiment during the charge/discharge cycles,deoxygenation and Li/Ni antisite defects are calculated,revealing that the chains of oxygen vacancies will be energetically preferrable.It can be inferred that in the absence of oxygen atom in high delithiate state,the diffusion of Ni atoms is facilitated and the formation of Li/Ni antisite is induced.展开更多
文摘The main raw material utilized in wood adhesives comes from petrochemical extractives.However,due to the excessive dependence on petrochemical resources and the adverse impact on the ecosystem and human wellbeing,there is an increasing trend to develop byproduct protein-based adhesives in the current global food safety context.In this research,flaxseed meal was subjected to pretreatment,and trimethylolpropane triglycidyl ether(TTE)and ethylenediamine(EN)were utilized as crosslinkers to establish a more compact adhesive layer and to prevent water intrusion.The pretreatment decreased the FM/UB viscosity by 60%compared to FM.The combination of CD analysis indicated that the Urea-NaOH pretreatment effectively stretched the flaxseed meal protein.According to Fourier transform infrared(FTIR)spectroscopy,X-ray diffraction(XRD),and differential scanning calorimetry(DSC)were used to analyze the resulting adhesive’s reaction mechanism and thermal response.Furthermore,the physical properties of the adhesive were characterized using wet shear strength testing and SEM observation.Remarkably,the dry bond strength increased from 0.72 to 2.12 MPa,representing a 194.4%increase.The wet bonding strength of the adhesive was improved from 0.22 to 1.21 MPa,representing a 550%increase compared to the original flaxseed protein-based adhesive,which far exceeded the minimum requirement for plywood of Type II(≥0.7 MPa,by GB/T 9846-2015).This study demonstrated an eco-friendly and sustainable method for the development of protein adhesives as viable substitutes for petrochemical resins.
基金supported by the Hubei Province Natural ScienceFoundation(No.2022CFC010).
文摘Backgrounds and Objective Angiopoietin-2(Ang-2)is a promising biomarker and therapeutic target for gastrointestinal angiodysplasia(GIAD).We hypothesized that the lncRNA-HIF1A-AS2/miR-153-3p/HIF-1α/Ang-2 axis plays a critical role in small bowel angiodysplasia(SBAD)-associated angiogenesis,which can be blocked by rifaximin.The purpose of this study was to investigate the expression and pro-angiogenic effects of the lncRNA-HIF1A-AS2/miR-153-3p/HIF-1α/Ang-2 in SBAD and to evaluate the therapeutic potential of rifaximin on SBAD by targeting this axis.Methods The expression and pro-angiogenic effects of lncRNA-HIF1A-AS2/miR-153-3p/HIF-1α/Ang-2 were analysed in SBAD tissues and human umbilical vein endothelial cells(HUVECs).The anti-angiogenic effect of rifaximin and its impact on the lncRNA-HIF1A-AS2/miR-153-3p/HIF-1α/Ang-2 axis were evaluated in HUVECs.Results Increased expression of lncRNA-HIF1A-AS2 and decreased expression of miR-153-3p were detected in SBAD tissues.LncRNA-HIF1A-AS2/miR-153-3p/HIF-1αwere upstream regulators of Ang-2,and this axis was involved in angiogenesis in HUVECs.Rifaximin exerted antiangiogenic effects on HUVECs by blocking this axis.Conclusions The lncRNA-HIF1A-AS2/miR-153-3p/HIF-1α/Ang-2 axis is critically involved in SBAD-associated angiogenesis.Rifaximin is a potential therapeutic option for SBAD via blockade of this axis.
文摘采用电化学方法研究了深冷处理温度对AZ31镁合金CMT(cold metal transfer)焊接接头腐蚀行为的影响。结果表明:与未深冷时相比,经–100℃,4h;–140℃,4h;–180℃,4h 3组参数深冷处理后的接头焊缝区耐蚀性能得到不同程度提高,随着深冷温度降低,耐蚀性能呈先升高后下降的变化趋势,深冷处理对焊缝区中第二相的尺寸、含量及分布的改变是决定这一趋势的关键因素。其中经–140℃,4h深冷处理的焊缝区电荷转移电阻和腐蚀产物膜电阻最大,腐蚀电流最低,耐蚀性能最佳。
基金Project of Orchid Biodiversity Survey of China from National Forestry and Grassland AdministrationNational Wild Plant Germplasm Resource Center for all kinds of support+1 种基金supported by the grants from the Second Tibetan Plateau Scientific Expedition and Research(STEP)program(2019QZKK0502)the National Natural Science Foundation of China(31970211)。
文摘Corallorhiza sinensis,a new species of mycoheterotrophic orchid from western Sichuan,China,is described and illustrated based on molecular and morphological evidence.It is morphologically similar to Corallorhiza trifida,but can be distinguished by bigger flowers,both sepals and petals with 3 veins,and longer lateral lobes of lip.To distinguish the new Corallorhiza species and explore its phylogenetic position within subtribe Calypsoinae,this study employed sequences of the nuclear ribosomal DNA(nrDNA)and whole plastome assembled from the genome skimming approach.The plastome is 148,124 bp in length,including a pair of inverted repeats(IRs)of 26,165 bp,a large single-copy region(LSC)of 82,207 bp,and a small single-copy region(SSC)of 13,587 bp.Further,phylogenetic analyses were performed using nrDNA sequence and 79 coding sequences(CDSs)from 26 complete plastomes of subtribe Calypsoinae.The phylogenetic tree based on nrDNA sequence suggested that Corallorhiza is a monophyletic group,and strongly support C.sinensis as sister to the rest species of Corallorhiza.The plastid tree showed that 10 Corallorhiza species grouped into two clades and C.sinensis is most closely related to the North American C.striata and C.bentleyi instead of Oreorchis foliosa and O.angustata in the same clade.The plastid tree and nrDNA tree indicate Oreorchis is a paraphyletic.Although the topological conflicts are displayed between plastome and nrDNA phylogenies of C.sinensis,it is still the most closely related to Corallorhiza.Comparative analysis showed that C.sinensis populations are characteristic of the intermediate morphological traits between Corallorhiza and Oreorchis.The finding of this new species from China shed new light on the phylogeny of Oreorchis and Corallorhiza.
基金supported by the National Natural Science Foundation of China(Grant No.51875253 and No.51935012)the Jiangsu Provincial Key Research and Development Program(BE2022069-2)the Western Light Project of Chinese Academy of Sciences(sbzg-zdsys-202007)。
文摘Complex-shaped optical lenses are of great interest in the areas of laser processing,machine vision,and optical communications.Traditionally,the processing of complex optical lenses is usually achieved by precision machining combined with post-grinding or polishing,which is expensive,labor-intensive and difficult in the processing of ultra-complex optical lenses.Additive manufacturing is an emerging technology that provides significant advantages in producing highly intricate optical devices.However,the layer-by-layer method employed in such manufacturing processes has resulted in low printing speeds,as well as limitations in surface quality.To address these challenges,we apply tomographic volumetric printing(TVP)in this work,which can realize the integrated printing of complex structural models without layering.By coordinating the TVP and the meniscus equilibrium post-curing methods,ultra-fast fabrication of complex-shaped lenses with sub-nanometric roughness has been achieved.A2.5 mm high,outer diameter 9 mm spherical lens with a roughness value of RMS=0.3340 nm is printed at a speed of 3.1×10^(4)mm^(3)h^(-1).As a further demonstration,a complex-shaped fly-eye lens is fabricated without any part assembly.The designed spherical lens is mounted on a smartphone’s camera,and the precise alignments above the circuit board are captured.Upon further optimization,this new technology demonstrates the potential for rapid fabrication of ultra-smooth complex optical devices or systems.
基金supports to Q.Chen from the National Basic Research Program of China(973 Program,No.2007CB513100)national science and technology project(2008ZX-10004-011)NSFC grant to J.Yin (30771886),N.Jiang(81171592,)and Q.Chen(81130033)
文摘Objective:To analyse the rar gene repertoire and characterise the rhondroitin sulphate A (CSA)-binding activity of the Duffy-binding like(I)BI.) domains encoded by the var2csa gene of a Plasmodium falciparum(P.falciparum) isolate in Hainan Province,China.Methods:The sequences of var DBL1 regions were PCR-amplified,sequenced and the sequence characteristics was bioinformalically analysed.Recombinant proteins encoded by the var2csa genes were expressed and purified.The binding activities of the recombinant proteins to CSA receptor was detected by ELISA assays.Results:Fifty six unique DBI.a sequences were obtained,and the sequences represented similar diversity to the var genes of the genome parasite 3D7.There are two var2csa genes in the P.falciparum isolated from Hainan Province.Unlike in other falciparum parasites such as HB3,the two var2csa genes are more diverged.The receptor-binding capacity of DBL-5εand DBI.-6 e domains of HN var2CSA was studied.Conclusions:This work represented the diversity of rar genes of a P.falciparum isolate in China.
基金Project supported by the Science Fund of the Guangdong Major Project of Basic and Applied Basic Research,China(Grant No.2019B030302011)the Fund of the Science and Technology Program of Guangzhou,China(Grant No.202201010090)。
文摘Combining the first-principles calculations and structural enumeration with recognition,the delithiation process of LiNiO_(2)is investigated,where various supercell shapes are considered in order to obtain the formation energy of Li_(x)NiO_(2).Meanwhile,the voltage profile is simulated and the ordered phases of lithium vacancies corresponding to concentrations of 1/4,2/5,3/7,1/2,2/3,3/4,5/6,and 6/7 are predicted.To understand the capacity decay in the experiment during the charge/discharge cycles,deoxygenation and Li/Ni antisite defects are calculated,revealing that the chains of oxygen vacancies will be energetically preferrable.It can be inferred that in the absence of oxygen atom in high delithiate state,the diffusion of Ni atoms is facilitated and the formation of Li/Ni antisite is induced.
