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枇杷bHLH基因家族特征和EjbHLH104调控三倍体枇杷花期的功能分析
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作者 夏燕 胡若倩 +7 位作者 易广权 孙洪文 黄树欣 池卓恒 石敏 梁国鲁 景丹龙 郭启高 《园艺学报》 北大核心 2025年第10期2582-2596,共15页
从枇杷(Eriobotrya japonica)基因组中鉴定到169个EjbHLH基因家族成员,这些基因在染色体上呈现不均等分布,划分为22个亚家族。采用RT-PCR技术克隆获得1个在早花三倍体枇杷‘无核早玉’花芽分化期显著高表达的bHLH成员EjbHLH104,其编码... 从枇杷(Eriobotrya japonica)基因组中鉴定到169个EjbHLH基因家族成员,这些基因在染色体上呈现不均等分布,划分为22个亚家族。采用RT-PCR技术克隆获得1个在早花三倍体枇杷‘无核早玉’花芽分化期显著高表达的bHLH成员EjbHLH104,其编码区序列长度为675 bp,编码224个氨基酸。蛋白质序列比对和分子系统发生分析表明,EjbHLH104具有典型且保守的bHLH保守结构域,与苹果MdbHLH104和白梨PbbHLH104等蔷薇科植物bHLH蛋白质的相似性较高,聚在真蔷薇分支Ⅰ。亚细胞定位分析表明,EjbHLH104蛋白质定位在细胞核,具有典型的bHLH转录因子亚细胞定位特性。实时荧光定量PCR分析表明,EjbHLH104基因在三倍体枇杷早花品种的花芽生理分化期和小花分化期表达量较高,在盛花期最高;而在二倍体早花品种的小花分化期表达量最高。EjbHLH104转基因拟南芥植株开花时间比野生型拟南芥显著提前,表明EjbHLH104基因过量表达具有促进拟南芥提前开花的功能。 展开更多
关键词 枇杷 三倍体 bHLH基因家族 功能分析 花期调控
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Application of Elastic Bandage in Reducing the Accidental Shedding of a Deep Vein Temporary Dialysis Catheter
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作者 WUKongju shimin +2 位作者 AOLi WANGYongfang WUXianfan 《外文科技期刊数据库(文摘版)医药卫生》 2022年第7期034-036,共3页
Objective: to consider the clinical value of elastic bandage in reducing the accidental shedding of deep venous temporary dialysis catheter. Methods: 200 patients received during Jan., 2019- Jan., 2020 and 200 patient... Objective: to consider the clinical value of elastic bandage in reducing the accidental shedding of deep venous temporary dialysis catheter. Methods: 200 patients received during Jan., 2019- Jan., 2020 and 200 patients received during Feb., 2020-Feb., 2022 were fixed by the pressure-sensitive tape and by the elastic bandage. Then, the risk of accidental loss is of intravenous temporary dialysis catheter in the 2 groups. Results: in the control group, 4 patients fell off accidentally, accounting for 2.00% and 0 cases, accounting for 0.00%. When the two groups were very different, there were obvious statistical advantages, P 0.05. Conclusion: elastic bandage can greatly reduce the risk of deep vein catheter accidental loss, reduce the emergence of some adverse reactions during treatment, but also can avoid skin allergies, gradually enhance the clinical comfort of patients, thus showing the strong humanized service characteristics. Therefore, this is a relatively human and safe and effective fixed method, is worth widely used in clinical practice. 展开更多
关键词 stretch bandage temporary dialysis of deep vein accidental loss of catheter APPLICATION
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