Objective:Menstrual blood-derived stem cells from endometriosis patients(E-MenSCs)have different gene expression patterns than those from healthy nonendometriotic females(NE-MenSCs).Exosomes extracted from mesenchymal...Objective:Menstrual blood-derived stem cells from endometriosis patients(E-MenSCs)have different gene expression patterns than those from healthy nonendometriotic females(NE-MenSCs).Exosomes extracted from mesenchymal stem cells and plants are considered for the treatment of various diseases.This study aimed to compare the effects of exosomes derived from NE-MenSCs(C-exos)and those from the roots of ginger(P-exos)on E-MenSCs.Methods:E-MenSCs at the third passage were used,and after evaluating the effective dosage with MTT,C-exos(200µg/mL)or P-exos(100µg/mL)were added to treat them.Following a 72-h incubation,the cells were analyzed with annexin V/PI test to evaluate the apoptosis rate.Also,genes related to inflammation(IL-6,IL-8,IL-1β,NF-κB,COX2),cell cycle(Cyclin D1),the steroid pathway(ESR1),migration and invasion(MMP-2,MMP-9,VEGF),and the apoptosis pathway(BAX,BCL2)were detected by real-time PCR.Results:Apoptosis was increased in both the P-and C-exos groups.The expression levels of IL-6 and IL-1βwere significantly lower in the P-exos group than in the E-MenSCs group.The expression levels of IL-8,NF-κB,COX-2,and MMP-9 were significantly decreased in both the P-exos group and the C-exos group.The expression level of VEGF was significantly lower in the P-exos group than in the E-MenSCs group.The BAX/BCL2 ratio was much lower in the P-exos group than in the E-MenSCs group.Conclusion:In this study,we established the feasibility of using a novel natural nontoxic material to target endometriotic mesenchymal stem cells to modify their gene expression and function toward healthy cells.Both C-exos and P-exos showed positive effects on the gene expression and function of endometriotic cells.Considering that plant exosomes are easier to access and less expensive,they can be considered for clinical use in improving the symptoms of endometriosis patients.展开更多
Exosomes are small vesicles that carry molecules from one cell to another.They have many features that make them interesting for research,such as their stability,low immunogenicity,size of the nanoscale,toxicity,and s...Exosomes are small vesicles that carry molecules from one cell to another.They have many features that make them interesting for research,such as their stability,low immunogenicity,size of the nanoscale,toxicity,and selective delivery.Exosomes can also interact with viruses in diverse ways.Emerging research highlights the significant role of exosomes in viral infections,particularly in the context of diseases like COVID-19,HIV,HBV and HCV.Understanding the intricate interplay between exosomes and the human immune system holds great promise for the development of effective antiviral therapies.An important aspect is gaining clarity on how exosomes influence the immune system and enhance viral infectivity through their inherent characteristics.By leveraging the innate properties of exosomes,viruses exploit the machinery involved in exosome biogenesis to set replication,facilitate the spread of infection,and eliminate immune responses.They can either help or hinder viral infection by modulating the immune system.This review summarizes the recent findings on how exosomes mediate viral infection and how they can be used for diagnosis or therapy.This could lead to new clinical applications of exosomes in disease management.展开更多
文摘Objective:Menstrual blood-derived stem cells from endometriosis patients(E-MenSCs)have different gene expression patterns than those from healthy nonendometriotic females(NE-MenSCs).Exosomes extracted from mesenchymal stem cells and plants are considered for the treatment of various diseases.This study aimed to compare the effects of exosomes derived from NE-MenSCs(C-exos)and those from the roots of ginger(P-exos)on E-MenSCs.Methods:E-MenSCs at the third passage were used,and after evaluating the effective dosage with MTT,C-exos(200µg/mL)or P-exos(100µg/mL)were added to treat them.Following a 72-h incubation,the cells were analyzed with annexin V/PI test to evaluate the apoptosis rate.Also,genes related to inflammation(IL-6,IL-8,IL-1β,NF-κB,COX2),cell cycle(Cyclin D1),the steroid pathway(ESR1),migration and invasion(MMP-2,MMP-9,VEGF),and the apoptosis pathway(BAX,BCL2)were detected by real-time PCR.Results:Apoptosis was increased in both the P-and C-exos groups.The expression levels of IL-6 and IL-1βwere significantly lower in the P-exos group than in the E-MenSCs group.The expression levels of IL-8,NF-κB,COX-2,and MMP-9 were significantly decreased in both the P-exos group and the C-exos group.The expression level of VEGF was significantly lower in the P-exos group than in the E-MenSCs group.The BAX/BCL2 ratio was much lower in the P-exos group than in the E-MenSCs group.Conclusion:In this study,we established the feasibility of using a novel natural nontoxic material to target endometriotic mesenchymal stem cells to modify their gene expression and function toward healthy cells.Both C-exos and P-exos showed positive effects on the gene expression and function of endometriotic cells.Considering that plant exosomes are easier to access and less expensive,they can be considered for clinical use in improving the symptoms of endometriosis patients.
文摘Exosomes are small vesicles that carry molecules from one cell to another.They have many features that make them interesting for research,such as their stability,low immunogenicity,size of the nanoscale,toxicity,and selective delivery.Exosomes can also interact with viruses in diverse ways.Emerging research highlights the significant role of exosomes in viral infections,particularly in the context of diseases like COVID-19,HIV,HBV and HCV.Understanding the intricate interplay between exosomes and the human immune system holds great promise for the development of effective antiviral therapies.An important aspect is gaining clarity on how exosomes influence the immune system and enhance viral infectivity through their inherent characteristics.By leveraging the innate properties of exosomes,viruses exploit the machinery involved in exosome biogenesis to set replication,facilitate the spread of infection,and eliminate immune responses.They can either help or hinder viral infection by modulating the immune system.This review summarizes the recent findings on how exosomes mediate viral infection and how they can be used for diagnosis or therapy.This could lead to new clinical applications of exosomes in disease management.
