The persistence and performance (growth promoting potential) of green fluorescent protein (gfp) marked Azotobacter chroococcum strain ABR 4G were studied in sterilized and unsterilized wheat rhizospheric soil. The gfp...The persistence and performance (growth promoting potential) of green fluorescent protein (gfp) marked Azotobacter chroococcum strain ABR 4G were studied in sterilized and unsterilized wheat rhizospheric soil. The gfp was integrated via Tn 5 transposition into A. chroococcum chromosome and the resultant gfp marked colonies were identified by green fluorescent emission under UV light. The gfp was stably maintained in A. chroococcum and the gfp insertion had no apparent adverse effect on the growth promoting properties of the marked soil isolate ABR 4G. The growth promoting properties (nitrogen fixation, ammonia excretion, phosphate solubilization and IAA production) of the parent soil isolate and the gfp marked strain were found to be almost the same. All the quantitative wheat plant traits were significantly influenced by inoculation of A. chroococcum ABR 4G strain in sterilized and unsterilized soil. Inoculated bacterial counts increased gradually in wheat rhizosphere, reached maximum on 60 th d and declined on 80 th d. Fertility levels also affected survival of marked strain and the survival was comparable in sterilized and unsterilized soil. The growth promoting properties were also determined from the marked strain reisolated from wheat rhizosphere in both types of soil. Fig 1, Tab 2, Ref展开更多
Background. The granular cell tumor (GCT) of vulva is a rare female genital t ract tumor. Case. A 59- year-old female was incidentally noted to have a 1- cm sized lump in her left labium majus that subsequently increa...Background. The granular cell tumor (GCT) of vulva is a rare female genital t ract tumor. Case. A 59- year-old female was incidentally noted to have a 1- cm sized lump in her left labium majus that subsequently increased in size to ap proximately 4 cm. The mass was totally excised under general anesthesia. Her pos toperative follow-up was uneventful. Histologic examination of the tumor showe d sheets and clusters of infiltrating tumor cells with morphologic features cons istent with granular cell tumor. Conclusion. Although benign and slow growing, i t has a tendency for recurrence and can cause morbidity and mortality when prese nting with multicentric or multiple organ involvement due to the lack of effecti ve systemic therapy. Therefore, clinicians and pathologists should be aware of i ts clinical and histopathologic features.展开更多
文摘The persistence and performance (growth promoting potential) of green fluorescent protein (gfp) marked Azotobacter chroococcum strain ABR 4G were studied in sterilized and unsterilized wheat rhizospheric soil. The gfp was integrated via Tn 5 transposition into A. chroococcum chromosome and the resultant gfp marked colonies were identified by green fluorescent emission under UV light. The gfp was stably maintained in A. chroococcum and the gfp insertion had no apparent adverse effect on the growth promoting properties of the marked soil isolate ABR 4G. The growth promoting properties (nitrogen fixation, ammonia excretion, phosphate solubilization and IAA production) of the parent soil isolate and the gfp marked strain were found to be almost the same. All the quantitative wheat plant traits were significantly influenced by inoculation of A. chroococcum ABR 4G strain in sterilized and unsterilized soil. Inoculated bacterial counts increased gradually in wheat rhizosphere, reached maximum on 60 th d and declined on 80 th d. Fertility levels also affected survival of marked strain and the survival was comparable in sterilized and unsterilized soil. The growth promoting properties were also determined from the marked strain reisolated from wheat rhizosphere in both types of soil. Fig 1, Tab 2, Ref
文摘Background. The granular cell tumor (GCT) of vulva is a rare female genital t ract tumor. Case. A 59- year-old female was incidentally noted to have a 1- cm sized lump in her left labium majus that subsequently increased in size to ap proximately 4 cm. The mass was totally excised under general anesthesia. Her pos toperative follow-up was uneventful. Histologic examination of the tumor showe d sheets and clusters of infiltrating tumor cells with morphologic features cons istent with granular cell tumor. Conclusion. Although benign and slow growing, i t has a tendency for recurrence and can cause morbidity and mortality when prese nting with multicentric or multiple organ involvement due to the lack of effecti ve systemic therapy. Therefore, clinicians and pathologists should be aware of i ts clinical and histopathologic features.
