Polyphenols from the ethanol extracts of Sargassum tenerrimum （ST） with potent antiallergic effects were studied to optimize separation process through column chromatography. The adsorption and desorption characteri...Polyphenols from the ethanol extracts of Sargassum tenerrimum （ST） with potent antiallergic effects were studied to optimize separation process through column chromatography. The adsorption and desorption characteristics of three widely used adsorbents： macroporous resin, silica gel, and polyvinylpolypyrrolidone （PVPP）, were critically evaluated respectively and studied for the optimization of preparative separation of polyphenols. Static operations on these adsorbents showed that macroporous resin had the best adsorption and desorption capability among the three adsorbents. Dynamic adsorption and desorption with macroporous resin packed column were also conducted to optimize the parameters such as： with the optimal values shown in brackets, the concen- tration of extract solution （4 times diluted）, pH value （6-7）, adsorption speed （3BVh-1, bed volumes/per hour）, concentration of ethanol （80%）, elution speed （3 BV ht） and elution volume （7 BV）. The chromatographic process so optimized gave a purity of 62.43% from the crude polyphenols, providing a promising basis for large scale preparation of bioactive polyphenols upon further scaling up tests.展开更多
In order to better understand shrimp allergen,some basic characters of the major allergen of greasy-back shrimp(Metapenaeus ensis)were investigated.The major allergen was extracted and separated,and its peptide mass f...In order to better understand shrimp allergen,some basic characters of the major allergen of greasy-back shrimp(Metapenaeus ensis)were investigated.The major allergen was extracted and separated,and its peptide mass fingerprint(PMF)wasanalyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS).After screening inthe NCBI database with Mascot searching engine,the results indicated that the major allergen of greasy-back shirmp was muscletropomyosin.Database matching search showed that the top protein matched,i.e.the tropomyosin from giant tiger prawn(Penaeusmonodon),had a Mowse value of 268.In addition,there were 27 queries matched with the allergen in greasy-back shirmp with anamino acid sequence coverage value of 65%.The matching scores and the sequence coverage values were also high with tropomy-osins of other invertebrates,including Tyrophagus putrescentiae and Lepisma saccharina.These results indicated that the allergen ofMetapenaeus ensis had high homology with other crustacean allergens,and provided molecular explanations for the highcross-reactivity of the major allergens between crustaceans and some other invertebrates.展开更多
基金supported by the National High Technology Research and Development Program ofChina (863 Program) (2006AA09Z427)NSFC (Nos. 30800859 and 30871948)
文摘Polyphenols from the ethanol extracts of Sargassum tenerrimum （ST） with potent antiallergic effects were studied to optimize separation process through column chromatography. The adsorption and desorption characteristics of three widely used adsorbents： macroporous resin, silica gel, and polyvinylpolypyrrolidone （PVPP）, were critically evaluated respectively and studied for the optimization of preparative separation of polyphenols. Static operations on these adsorbents showed that macroporous resin had the best adsorption and desorption capability among the three adsorbents. Dynamic adsorption and desorption with macroporous resin packed column were also conducted to optimize the parameters such as： with the optimal values shown in brackets, the concen- tration of extract solution （4 times diluted）, pH value （6-7）, adsorption speed （3BVh-1, bed volumes/per hour）, concentration of ethanol （80%）, elution speed （3 BV ht） and elution volume （7 BV）. The chromatographic process so optimized gave a purity of 62.43% from the crude polyphenols, providing a promising basis for large scale preparation of bioactive polyphenols upon further scaling up tests.
基金supported by the Nature Science Foundation of China(No.30800859 and No.30871948)the Key Projects in the National Science and Technology Pillar Program during the Eleventh Five-year Plan Period(2008BAD94B09)
文摘In order to better understand shrimp allergen,some basic characters of the major allergen of greasy-back shrimp(Metapenaeus ensis)were investigated.The major allergen was extracted and separated,and its peptide mass fingerprint(PMF)wasanalyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS).After screening inthe NCBI database with Mascot searching engine,the results indicated that the major allergen of greasy-back shirmp was muscletropomyosin.Database matching search showed that the top protein matched,i.e.the tropomyosin from giant tiger prawn(Penaeusmonodon),had a Mowse value of 268.In addition,there were 27 queries matched with the allergen in greasy-back shirmp with anamino acid sequence coverage value of 65%.The matching scores and the sequence coverage values were also high with tropomy-osins of other invertebrates,including Tyrophagus putrescentiae and Lepisma saccharina.These results indicated that the allergen ofMetapenaeus ensis had high homology with other crustacean allergens,and provided molecular explanations for the highcross-reactivity of the major allergens between crustaceans and some other invertebrates.
