Fish roes,rich in sialoglycoproteins,are often discarded,raising environmental concerns.The current study aimed to evaluate the antihypertensive effects of sialoglycoprotein hydrolysate produced from Labeo rohita roes...Fish roes,rich in sialoglycoproteins,are often discarded,raising environmental concerns.The current study aimed to evaluate the antihypertensive effects of sialoglycoprotein hydrolysate produced from Labeo rohita roes using Wistar rats.SGPH exhibited moderate but significant ACE-inhibitory activity(74.70±0.13%),DPPH radical scavenging activity(37.85±1.08%),ABTS radical scavenging activity(47.15±1.60%),and FRAP activity(27.13±0.03μM Trolox equivalents/g).The ACE-inhibition kinetics and gastrointestinal(GI)digestibility of SGPH demonstrated that SGPH was a competitive inhibitor and relatively stable in simulated GI conditions.SGPH revealed a moderate but significant antihypertensive effect on hypertensive(2K1C)Wistar rats in a dose-dependent manner by reducing systolic and diastolic blood pressure(SBP and DBP)and kidney ACE activity(P<0.05)after 4 weeks of treatment.Reduction of SBP,DBP,and kidney ACE activity ranged from 140±2-128±2 mmHg,100±3-82±3 mmHg,and 0.94±0.01-0.61±0.02μM HA/min/mg,respectively.Notably,SGPH elevated the superoxide dismutase and catalase activities in 2K1C rats(P<0.05),irrespective of SGPH dose,which could augment additional cardio-protection by minimizing oxidative stress.SGPH-treated rat groups displayed urine,haematology and serum biochemistry values in the normal range,indicating no adverse effects of SGPH.Further,histopathological evaluation elucidated that there was no incidence of organ abnormality in SGPH-treated rats,asserting that SGPH precluded vital organ injuries.Therefore,SGPH could be a promising antihypertensive ingredient that can be deployed in functional foods/nutraceuticals development for combating hypertension and its related diseases.展开更多
基金Council of Scientific and Industrial Research(CSIR),New Delhi,India,for the research grant(CFTRI MLP-290)AV acknowledges the University Grants Commission,New Delhi,India,for the award of the CSIR-UGC fellowship.
文摘Fish roes,rich in sialoglycoproteins,are often discarded,raising environmental concerns.The current study aimed to evaluate the antihypertensive effects of sialoglycoprotein hydrolysate produced from Labeo rohita roes using Wistar rats.SGPH exhibited moderate but significant ACE-inhibitory activity(74.70±0.13%),DPPH radical scavenging activity(37.85±1.08%),ABTS radical scavenging activity(47.15±1.60%),and FRAP activity(27.13±0.03μM Trolox equivalents/g).The ACE-inhibition kinetics and gastrointestinal(GI)digestibility of SGPH demonstrated that SGPH was a competitive inhibitor and relatively stable in simulated GI conditions.SGPH revealed a moderate but significant antihypertensive effect on hypertensive(2K1C)Wistar rats in a dose-dependent manner by reducing systolic and diastolic blood pressure(SBP and DBP)and kidney ACE activity(P<0.05)after 4 weeks of treatment.Reduction of SBP,DBP,and kidney ACE activity ranged from 140±2-128±2 mmHg,100±3-82±3 mmHg,and 0.94±0.01-0.61±0.02μM HA/min/mg,respectively.Notably,SGPH elevated the superoxide dismutase and catalase activities in 2K1C rats(P<0.05),irrespective of SGPH dose,which could augment additional cardio-protection by minimizing oxidative stress.SGPH-treated rat groups displayed urine,haematology and serum biochemistry values in the normal range,indicating no adverse effects of SGPH.Further,histopathological evaluation elucidated that there was no incidence of organ abnormality in SGPH-treated rats,asserting that SGPH precluded vital organ injuries.Therefore,SGPH could be a promising antihypertensive ingredient that can be deployed in functional foods/nutraceuticals development for combating hypertension and its related diseases.
