Phytophthora pathogens secrete numerous apoplastic effectors to manipulate host immunity.Herein,we identified a polysaccharide lyase 1 protein,PsPL1,which acts as an essential virulence factor of P.sojae infection in ...Phytophthora pathogens secrete numerous apoplastic effectors to manipulate host immunity.Herein,we identified a polysaccharide lyase 1 protein,PsPL1,which acts as an essential virulence factor of P.sojae infection in soybean.However,the overexpression of PsPL1 in P.sojae reduced infection and triggered enhanced immune responses in soybean.PsPL1 exhibited pectin lyase activity and degraded plant pectin to generate pectin oligosaccharides(POSs)with a polymerization degree of 3–14,exhibiting different levels of acetylation and methylation modifications.PsPL1 and the degraded pectin products triggered immune responses in soybean and different Solanaceous plants.The PsPL1-triggered immune responses required RSPL1,a membrane-localized leucine-rich repeat receptor-like protein,which is essential for Phytophthora resistance.Conversely,the PsPL1-degraded product-triggered immune responses depended on the membrane-localized lysin motif receptor-like kinase CERK1.This study reveals that the pectin lyase exhibits a dual immunogenic role during P.sojae infection,which activates plant resistance through different immune receptors and provides novel insights into the function of pectin lyase in host-pathogen interactions.展开更多
基金supported by the Natural Science Foundation of Jiangsu Province (BK20210386)the Fundamental Research Funds for the Central Universities (CGPY2024001)+6 种基金the National Natural Science Foundation of China (32102233,32270210,32102172)the Innovation Fund of DICP (DICPI202412)the China Postdoctoral Science Foundation (2024T170421)Jiangsu Funding Program for Excellent Postdoctoral Talent (2023ZB236)the Postdoctoral Fellowship Program of CPSF (GZC20231129)the Key Research and Development Program of Guangdong Province (2022B0202080004)China Agriculture Research System (CARS-004-PS14)。
文摘Phytophthora pathogens secrete numerous apoplastic effectors to manipulate host immunity.Herein,we identified a polysaccharide lyase 1 protein,PsPL1,which acts as an essential virulence factor of P.sojae infection in soybean.However,the overexpression of PsPL1 in P.sojae reduced infection and triggered enhanced immune responses in soybean.PsPL1 exhibited pectin lyase activity and degraded plant pectin to generate pectin oligosaccharides(POSs)with a polymerization degree of 3–14,exhibiting different levels of acetylation and methylation modifications.PsPL1 and the degraded pectin products triggered immune responses in soybean and different Solanaceous plants.The PsPL1-triggered immune responses required RSPL1,a membrane-localized leucine-rich repeat receptor-like protein,which is essential for Phytophthora resistance.Conversely,the PsPL1-degraded product-triggered immune responses depended on the membrane-localized lysin motif receptor-like kinase CERK1.This study reveals that the pectin lyase exhibits a dual immunogenic role during P.sojae infection,which activates plant resistance through different immune receptors and provides novel insights into the function of pectin lyase in host-pathogen interactions.
