In this study, a cellulose-degrading bacterium was isolated from paddy soils in Chengdu City. After 2 d of culture, the average diameter of hydrolytic circles on CMC-Congo red medium was 26 -30 mm. Single colonies on ...In this study, a cellulose-degrading bacterium was isolated from paddy soils in Chengdu City. After 2 d of culture, the average diameter of hydrolytic circles on CMC-Congo red medium was 26 -30 mm. Single colonies on LB plates were white and wrinkled. The isolated strain was identified as a Gram-negative bacterium by Gram staining, which could produce short red-like spores. Colony PCR was performed using the bacterial 16S rDNA universal primers. A 1 429 kb DNA fragment was amplified. Based on sequence homology analysis and comprehensive analysis, the isolated strain was identified as Bacillus velezensi, which was named CDBV62 according to the origin.展开更多
文摘In this study, a cellulose-degrading bacterium was isolated from paddy soils in Chengdu City. After 2 d of culture, the average diameter of hydrolytic circles on CMC-Congo red medium was 26 -30 mm. Single colonies on LB plates were white and wrinkled. The isolated strain was identified as a Gram-negative bacterium by Gram staining, which could produce short red-like spores. Colony PCR was performed using the bacterial 16S rDNA universal primers. A 1 429 kb DNA fragment was amplified. Based on sequence homology analysis and comprehensive analysis, the isolated strain was identified as Bacillus velezensi, which was named CDBV62 according to the origin.
