Objective To analyze the chemical constituents of Shanxiangyuanye(Turpiniae Folium)through liquid chromatography-tandem mass spectrometry(LC-MS/MS)method,and to evaluate their anti-oxidant,hypoglycemic,and anti-glycat...Objective To analyze the chemical constituents of Shanxiangyuanye(Turpiniae Folium)through liquid chromatography-tandem mass spectrometry(LC-MS/MS)method,and to evaluate their anti-oxidant,hypoglycemic,and anti-glycation activities related to diabetic complications.Methods The supernatant of Shanxiangyuanye(Turpiniae Folium)(TFS),obtained following water extraction and alcohol precipitation,was analyzed by LC-MS/MS.Antioxidant activity of TFS in vitro was evaluated using three experimental approaches:the 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging assay,the 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS+)radical cation decolorization assay,and the hydroxyl(·OH)radical scavenging assay.To comprehensively evaluate hypoglycemic potential,α-glucosidase inhibition was measured to analyze in vitro hypoglycemic activity.Subsequently,in vitro models were developed to examine anti-glycation activity through the bovine serum albumin(BSA)-fructose(Fru),BSA-methylglyoxal(MGO),BSA-glyoxal(GO),and D-arginine(Arg)-MGO systems,with particular attention to the inhibitory effects of TFS.Furthermore,the concentrations of fructosamine,protein carbonyls,sulfhydryl groups,andβ-amyloid in the glycation solution were quantified using the BSA-Fru model following 7-d of incubation at 37°C.Results Using LC-MS/MS analysis in both positive and negative ion modes,we identified 750 chemical components in TFS,primarily including organic acids,amino acids,and their derivatives.In vitro activity studies demonstrated that TFS exhibited remarkable free radical scavenging capacity,with half-maximal inhibitory concentrations(IC_(50))of 0.47,1.56,and 0.36 mg/mL against DPPH,ABTS+,and·OH radicals,respectively.Regarding hypoglycemic activity,TFS dose-dependently inhibitedα-glucosidase activity(IC_(50)=0.21 mg/mL),displaying comparable efficacy to the clinical drug acarbose(IC_(50)=0.23 mg/mL).Notably,TFS intervened in the glycation process:IC_(50) values were 0.22,1.91–4.96,and 4.09 mg/mL in the BSAFru,BSA-MGO/GO,and Arg-MGO models,respectively,with the most prominent inhibitory effects observed in the BSA-Fru model.Furthermore,although TFS may not effectively preserve thiol groups in BSA or reduce thiol oxidation during glycation,it significantly reduces fructosamine levels(in a dose-dependent manner),decreasesβ-amyloid formation,and inhibits protein carbonylation(P<0.0001).Conclusion The findings demonstrate that TFS exhibits a complex chemical composition with potent antioxidant,hypoglycemic,and anti-glycation activities.These results provide compelling scientific evidence supporting TFS’s potential as a natural adjuvant for diabetes prevention and complication management,while laying a solid foundation for its applications in functional food development and adjunctive antidiabetic therapeutics.展开更多
基金Key Project of Hunan Provincial Education Bureau(23A0307)Natural Science Foundation of Hunan Province (2025JJ80089)。
文摘Objective To analyze the chemical constituents of Shanxiangyuanye(Turpiniae Folium)through liquid chromatography-tandem mass spectrometry(LC-MS/MS)method,and to evaluate their anti-oxidant,hypoglycemic,and anti-glycation activities related to diabetic complications.Methods The supernatant of Shanxiangyuanye(Turpiniae Folium)(TFS),obtained following water extraction and alcohol precipitation,was analyzed by LC-MS/MS.Antioxidant activity of TFS in vitro was evaluated using three experimental approaches:the 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging assay,the 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS+)radical cation decolorization assay,and the hydroxyl(·OH)radical scavenging assay.To comprehensively evaluate hypoglycemic potential,α-glucosidase inhibition was measured to analyze in vitro hypoglycemic activity.Subsequently,in vitro models were developed to examine anti-glycation activity through the bovine serum albumin(BSA)-fructose(Fru),BSA-methylglyoxal(MGO),BSA-glyoxal(GO),and D-arginine(Arg)-MGO systems,with particular attention to the inhibitory effects of TFS.Furthermore,the concentrations of fructosamine,protein carbonyls,sulfhydryl groups,andβ-amyloid in the glycation solution were quantified using the BSA-Fru model following 7-d of incubation at 37°C.Results Using LC-MS/MS analysis in both positive and negative ion modes,we identified 750 chemical components in TFS,primarily including organic acids,amino acids,and their derivatives.In vitro activity studies demonstrated that TFS exhibited remarkable free radical scavenging capacity,with half-maximal inhibitory concentrations(IC_(50))of 0.47,1.56,and 0.36 mg/mL against DPPH,ABTS+,and·OH radicals,respectively.Regarding hypoglycemic activity,TFS dose-dependently inhibitedα-glucosidase activity(IC_(50)=0.21 mg/mL),displaying comparable efficacy to the clinical drug acarbose(IC_(50)=0.23 mg/mL).Notably,TFS intervened in the glycation process:IC_(50) values were 0.22,1.91–4.96,and 4.09 mg/mL in the BSAFru,BSA-MGO/GO,and Arg-MGO models,respectively,with the most prominent inhibitory effects observed in the BSA-Fru model.Furthermore,although TFS may not effectively preserve thiol groups in BSA or reduce thiol oxidation during glycation,it significantly reduces fructosamine levels(in a dose-dependent manner),decreasesβ-amyloid formation,and inhibits protein carbonylation(P<0.0001).Conclusion The findings demonstrate that TFS exhibits a complex chemical composition with potent antioxidant,hypoglycemic,and anti-glycation activities.These results provide compelling scientific evidence supporting TFS’s potential as a natural adjuvant for diabetes prevention and complication management,while laying a solid foundation for its applications in functional food development and adjunctive antidiabetic therapeutics.
