[Objectives]To analyze the microbial community structure and diversity in the rhizosphere soil of peach trees in the Tangshan area of Hebei Province,identify the dominant microbial groups,and explore their potential e...[Objectives]To analyze the microbial community structure and diversity in the rhizosphere soil of peach trees in the Tangshan area of Hebei Province,identify the dominant microbial groups,and explore their potential ecological functions.[Methods]Amplification sequencing analysis of bacterial and fungal communities in the rhizosphere soil of a peach orchard in Qian'an County,Tangshan City,Hebei Province,was performed using Illumina MiSeq high-throughput sequencing technology.[Results]The indices of Sobs,Chao,ACE,and Shannon for soil bacteria in the rhizosphere soil of peach trees were all higher than those for fungi,indicating a more uniform and diverse bacterial community structure.At the phylum level,the bacteria with relatively high abundance included Pseudomonadota(28.29%),Acidobacteriota(18.10%),Bacillota(12.17%),and Actinomycetota(11.73%).In contrast,the fungi with relatively high abundance were Ascomycota(64.64%),Basidiomycota(14.22%),and Mortierellomycota(14.09%).At the genus level,the bacteria with relatively high abundance comprised Sphingomonas(5.00%),Priestia(3.38%),Nitrospira(2.05%),etc.The fungi with relatively high abundance included Fusarium(13.13%),Mortierella(12.86%),Tausonia(6.97%),Neocosmospora(4.77%),etc.[Conclusions]This study offers a foundational dataset and theoretical reference for the regulation of rhizosphere microecology and the management of soil health in peach orchards in Tangshan.展开更多
In the present study, a method for the quantitative analysis of multi-components by single marker(QAMS) has been developed and validated for the simultaneous determination of echinacoside(ECH), tubuloside A, acteoside...In the present study, a method for the quantitative analysis of multi-components by single marker(QAMS) has been developed and validated for the simultaneous determination of echinacoside(ECH), tubuloside A, acteoside, isoacteoside, and2’-acetylacteoside in Cistanches Herba. ECH was used as the internal standard(IS) to obtain the relative correction factors(RCFs) of the other four phenylethanoid glycosides(PhGs);meanwhile, various influencing factors on RCFs were investigated under different conditions. The content of each component was calculated with RCF. The results were compared with those obtained by the external standard method(ESM) to verify the feasibility and accuracy of the established QAMS method. No significant difference was found in the quantitative results of 10 batches of Cistanches Herba between QAMS and ESM. The proposed QAMS method for simultaneous determination of PhGs in Cistanches Herba is accurate and feasible, providing an efficient and economical approach for the quality control of Cistanches Herba.展开更多
Background: Aneurysmal subarachnoid hemorrhage (aSAH) is an acute neurosurgical emergency with a significant fatality rate. In addition to acute brain injury, a considerable part of patients suffering from aSAH develo...Background: Aneurysmal subarachnoid hemorrhage (aSAH) is an acute neurosurgical emergency with a significant fatality rate. In addition to acute brain injury, a considerable part of patients suffering from aSAH develops secondary brain damage such as cerebral vasospasm (CVS). CVS exacerbates the mortality. Therefore, it is urgently needed to find a biomarker, which could predict secondary brain and lead to operation by physicians more promptly. S100B, produced and released by astrocytes, has proven to be an important biomarker for brain injury.Methods: In this present study, 51 patients with aSAH were included. Five CSF samples from each patient were obtained via lumbar puncture and were detected using electrochemiluminescence immunoassay (ECLIA).Results: It indicated that S100B had a higher concentration in CSF of patients treated by surgical clipping after aSAH than that treated with endovascular coiling. In addition, the mean CSF S100B level in patients without CVS was much lower compared with patients with CVS. And, the expression of S100B increased along with the Fisher Grade at the same day after aSAH attacked and decreased as time went on. Moreover, the CSF S100B level of different time points and the mean CSF S100B level can predict the risk of CVS.Conclusions: These data suggest that CSF S100B can be served as a predictor of CVS, which triggers an immediate management by clinicians to prevent secondary exacerbation.展开更多
Despite improvements in detection and treatment,advanced colorectal cancer(CRC)patients still face poor outcomes due to the complex anatomy of the colon and factors such as hypoxia,poor blood supply,and limited drug a...Despite improvements in detection and treatment,advanced colorectal cancer(CRC)patients still face poor outcomes due to the complex anatomy of the colon and factors such as hypoxia,poor blood supply,and limited drug access[1].These conditions create an immunosuppressive environment[2]and disrupt gut bacteria[3],reducing treatment effectiveness and enabling cancer evasion.Catalytic therapy offers promise by generating tu mor-killing reactive oxygen species(ROS)locally with minimal side effects,particularly via ultrasound-driven methods that penetrate deep tissues[4].However,single catalysts are hindered by inefficient charge separation,poor tumor targeting,and ROS neutralization in antioxidant-rich,oxygen-starved tumors[5].展开更多
文摘[Objectives]To analyze the microbial community structure and diversity in the rhizosphere soil of peach trees in the Tangshan area of Hebei Province,identify the dominant microbial groups,and explore their potential ecological functions.[Methods]Amplification sequencing analysis of bacterial and fungal communities in the rhizosphere soil of a peach orchard in Qian'an County,Tangshan City,Hebei Province,was performed using Illumina MiSeq high-throughput sequencing technology.[Results]The indices of Sobs,Chao,ACE,and Shannon for soil bacteria in the rhizosphere soil of peach trees were all higher than those for fungi,indicating a more uniform and diverse bacterial community structure.At the phylum level,the bacteria with relatively high abundance included Pseudomonadota(28.29%),Acidobacteriota(18.10%),Bacillota(12.17%),and Actinomycetota(11.73%).In contrast,the fungi with relatively high abundance were Ascomycota(64.64%),Basidiomycota(14.22%),and Mortierellomycota(14.09%).At the genus level,the bacteria with relatively high abundance comprised Sphingomonas(5.00%),Priestia(3.38%),Nitrospira(2.05%),etc.The fungi with relatively high abundance included Fusarium(13.13%),Mortierella(12.86%),Tausonia(6.97%),Neocosmospora(4.77%),etc.[Conclusions]This study offers a foundational dataset and theoretical reference for the regulation of rhizosphere microecology and the management of soil health in peach orchards in Tangshan.
基金National Key R&D Program of China(Grant Nos.2017YFC1702400,2018YFC1707300 and 2018YFC1707904)
文摘In the present study, a method for the quantitative analysis of multi-components by single marker(QAMS) has been developed and validated for the simultaneous determination of echinacoside(ECH), tubuloside A, acteoside, isoacteoside, and2’-acetylacteoside in Cistanches Herba. ECH was used as the internal standard(IS) to obtain the relative correction factors(RCFs) of the other four phenylethanoid glycosides(PhGs);meanwhile, various influencing factors on RCFs were investigated under different conditions. The content of each component was calculated with RCF. The results were compared with those obtained by the external standard method(ESM) to verify the feasibility and accuracy of the established QAMS method. No significant difference was found in the quantitative results of 10 batches of Cistanches Herba between QAMS and ESM. The proposed QAMS method for simultaneous determination of PhGs in Cistanches Herba is accurate and feasible, providing an efficient and economical approach for the quality control of Cistanches Herba.
文摘Background: Aneurysmal subarachnoid hemorrhage (aSAH) is an acute neurosurgical emergency with a significant fatality rate. In addition to acute brain injury, a considerable part of patients suffering from aSAH develops secondary brain damage such as cerebral vasospasm (CVS). CVS exacerbates the mortality. Therefore, it is urgently needed to find a biomarker, which could predict secondary brain and lead to operation by physicians more promptly. S100B, produced and released by astrocytes, has proven to be an important biomarker for brain injury.Methods: In this present study, 51 patients with aSAH were included. Five CSF samples from each patient were obtained via lumbar puncture and were detected using electrochemiluminescence immunoassay (ECLIA).Results: It indicated that S100B had a higher concentration in CSF of patients treated by surgical clipping after aSAH than that treated with endovascular coiling. In addition, the mean CSF S100B level in patients without CVS was much lower compared with patients with CVS. And, the expression of S100B increased along with the Fisher Grade at the same day after aSAH attacked and decreased as time went on. Moreover, the CSF S100B level of different time points and the mean CSF S100B level can predict the risk of CVS.Conclusions: These data suggest that CSF S100B can be served as a predictor of CVS, which triggers an immediate management by clinicians to prevent secondary exacerbation.
基金financially supported by the National Natural Science Foundation of China(32471388 and 32071322)。
文摘Despite improvements in detection and treatment,advanced colorectal cancer(CRC)patients still face poor outcomes due to the complex anatomy of the colon and factors such as hypoxia,poor blood supply,and limited drug access[1].These conditions create an immunosuppressive environment[2]and disrupt gut bacteria[3],reducing treatment effectiveness and enabling cancer evasion.Catalytic therapy offers promise by generating tu mor-killing reactive oxygen species(ROS)locally with minimal side effects,particularly via ultrasound-driven methods that penetrate deep tissues[4].However,single catalysts are hindered by inefficient charge separation,poor tumor targeting,and ROS neutralization in antioxidant-rich,oxygen-starved tumors[5].
