Peanuts are important oilseed legume crops that are susceptible to contamination by Aspergillus flavus in soil,leading to serious economic losses.Previously,our research team developed the Aspergillus-Rihizobia coupli...Peanuts are important oilseed legume crops that are susceptible to contamination by Aspergillus flavus in soil,leading to serious economic losses.Previously,our research team developed the Aspergillus-Rihizobia coupling(ARC)microbial inoculants and found it can reduce A.flavus abundance in the soil and promote efficient nodulation in peanuts.However,the impact of ARC microbial inoculants on different resistant varieties of A.flavus remains unclear.In this study,we screened peanut varieties that were resistant and susceptible to A.flavus and evaluated their nodulation ability and growth performance after ARC microbial inoculants treatment in the field.The results demonstrated that the nodule number and nitrogenase activity of both varieties significantly increased after ARC microbial inoculants treatment,with the highly susceptible variety AH24 showing a greater increase.For photosynthetic parameters,both varieties also increased after ARC microbial inoculants treatment,but the increase was greater in the moderately resistant variety AH1 than in the highly susceptible variety AH24.Finally,we found that the yield and yield-related traits of the moderately resistant variety AH1 were better than those of the highly susceptible variety AH24.After ARC microbial inoculants treatment,the yield traits of both peanut varieties still increased significantly,but the degree of increase of the moderately resistant variety AH1 was smaller than that of the highly susceptible variety AH24.In addition,the abundance of A.flavus in the rhizosphere soil of the two varieties significantly decreased after ARC microbial inoculants treatment,with no significant difference between the varieties.These results indicated that ARC microbial inoculants exert differential effects on the nodulation and growth of different resistant peanut varieties and have a better effect on highly susceptible varieties.These results provide a solid theoretical basis for the efficient use of ARC microbial inoculants in the field of peanuts in the future.展开更多
As important supplementary to major edible oils, comparative chemical advantages of minor edible oils decidetheir development and usage. In this study, chemical composition of 13 kinds of specific edible vegetable oil...As important supplementary to major edible oils, comparative chemical advantages of minor edible oils decidetheir development and usage. In this study, chemical composition of 13 kinds of specific edible vegetable oilswere investigated. The comparative advantages of chemical compositions of these edible oils were obtained asfollows: (1) camellia, tiger nut and almond oil were rich in oleic acid, the contents of which accounted for79.43%, 69.16% and 66.26%, respectively;(2) safflower oil contained the highest content of linoleic acid(76.69%), followed by grape seed (66.85%) and walnut oil (57.30%);(3) perilla seed, siritch, peony seed andherbaceous peony seed oil were rich in α-linolenic acid (59.61%, 43.74%, 40.83% and 30.84%, respectively);(4)the total phytosterol contents of these oils ranged from 91.46 mg/100 g (camellia oil) to 506.46 mg/100 g (siritchoil);and (5) The best source of tocopherols was sacha inchi oil (122.74 mg/100 g), followed by perilla seed oil(55.89 mg/100 g), peony seed oil (53.73 mg/100 g) and herbaceous peony seed oil (47.17 mg/100 g). Thecomparative advantages of these specific edible oils indicated that they possess the high potential nutritionalvalues and health care functions.展开更多
To evaluate antibacterial activities of edible vegetable oils in China for healthy consumption, we investigated the antibacterial activities of extracts from 6 edible vegetable oils and rapeseed oils at different refi...To evaluate antibacterial activities of edible vegetable oils in China for healthy consumption, we investigated the antibacterial activities of extracts from 6 edible vegetable oils and rapeseed oils at different refining levels on 4 bactria (Staphylococcus aureus,Bacillus subtilis, Escherichia coli and Pseudomonas strains) by disc diffusion test. Results showed that camellia oil had considerable antibacterial activity against E. coli with antibacterial rate of 108.3%. Perilla oil, sunflower seed oil, and flaxseed oil showed the highest antibacterial activities against B. subtilis with the antibacterial rates of 108.3%, 102.8%and94.4%respectively. Moreover, the extracts of rapeseed oils obtained by different processing technologies showed significantly different antibacterial effects. Two crude oils and grade4 rapeseed oil showed antibacterial rates of more than 40%, whereas grade 1 and 2 rapeseed oils showed antibacterial rates of less than 20%. Therefore, it was recommended to use rapeseed oil rather than refined rapeseed oil because of higher antibacterial activities. It was suggested that proper refining processes should be taken to obtain the edible oils with higher antibacterial activities.展开更多
In this study, anthocyanin and fatty acid compositions between black and ordinary peanuts were compared. Results showed that black peanuts contained 93.90±18.36 μg/g of cyanidin, which was significantly higher t...In this study, anthocyanin and fatty acid compositions between black and ordinary peanuts were compared. Results showed that black peanuts contained 93.90±18.36 μg/g of cyanidin, which was significantly higher than that of ordinary peanuts. In addition, although both of black and ordinary peanuts shared some specific fatty acids, 4 fatty acids in black peanuts were significantly different from those in ordinary peanuts, which could be used as markers to distinguish black peanut from ordinary ones. This study might serve as a reference for authenticity identification and further nutritional researches on black peanut.展开更多
Monochamus alternatus is the primary carrier of pine wood nematodes,which pose a serious threat to Pinus spp.in many countries.Newly emerging M.alternatus adults feed on heathy host pines,while matured adults transfer...Monochamus alternatus is the primary carrier of pine wood nematodes,which pose a serious threat to Pinus spp.in many countries.Newly emerging M.alternatus adults feed on heathy host pines,while matured adults transfer to stressed host pines for mating and oviposition.Several odorant-binding proteins(OBPs)of M.alternatus have been proved to aid in the complex process of host location.To clarify the corresponding relations between OBPs and pine volatiles,more OBPs need to be studied.In this research,MaltOBP19 showed a specific expression in the antennae and mouthparts of M.alternatus,and it was marked in 4 types of antenna sensilla by immunolocalization.Fluorescence binding assays demonstrated the high binding affinity of MaltOBP19 with camphene and myrcene in vitro.In Y-tube olfactory experiments,M.alternatus adults were attracted by camphene and RNAi of OBP19 via microinjection significantly decreased their attraction index.Myrcene induced phobotaxis,but RNAi had no significant effect on this behavior.Further,we found that ingesting dsOBP19 produced by a bacteria-expressed system with a newly constructed vector could lead to the knockdown of MaltOBP19.These results suggest that MaltOBP19 may play a role in the process of host conversion via the recognition of camphene,which has been identified to be strongly released in stressed host pines.In addition,it is proved that knockdown of OBP can be achieved by oral administration of bacteria-expressed double-stranded RNA in M.alternatus adults,providing a new perspective in the control of M.alternatus.展开更多
Encapsulation of phytosterols in nanostructured lipid carriers(NLCs)is an effective approach to improve their physicochemical properties,but the interaction of phytosterols in NLCs with cholesterol can be affected by ...Encapsulation of phytosterols in nanostructured lipid carriers(NLCs)is an effective approach to improve their physicochemical properties,but the interaction of phytosterols in NLCs with cholesterol can be affected by lipid matrices.The objective of this study was to screen a desired phytosterols-loaded NLC(PS-NLC)formulation to reduce the absorption of intestinal cholesterol.A series of formulations were prepared using amphipathic glycerol monostearate(GMS)as the solid lipid fraction to increase lipid compatibity with phytosterols.All formulations showed a lowering effect on cholesterol bioaccessibility through in vitro intestinal digestion.And the PS-NLCs with medium chain triglycerides(MCT)as the liquid lipid showed significantly higher inhibition of cholesterol bioaccessibility(accessible fraction for intestinal absorption)than the formulations with long chain triglycerides(LCT),which could due to the solubilization of cholesterol by hydrolysis products of LCT,suggesting a notable impact of lipid type on cholesterol bioaccessibility.Furthermore,the analysis of distribution of phytosterols and cholesterol revealed a cocrystallization mechanism occurred during in vitro digestion.This study provides a certain reference for designing phytosterol-ingredient agents with a better hypocholesterolemic effect in water-dispersible functional foods.展开更多
基金supported by the Agricultural Science and Technology Innovation Program(CAAS-ZDRW202416)the Foundation of Hubei Hongshan Laboratory(2021hszd015)+1 种基金the Science and Technology Major Projects of Hubei Province(2023BBA002)the Knowledge Innovation Program of Wuhan-Basi Research(2023020201010126)。
文摘Peanuts are important oilseed legume crops that are susceptible to contamination by Aspergillus flavus in soil,leading to serious economic losses.Previously,our research team developed the Aspergillus-Rihizobia coupling(ARC)microbial inoculants and found it can reduce A.flavus abundance in the soil and promote efficient nodulation in peanuts.However,the impact of ARC microbial inoculants on different resistant varieties of A.flavus remains unclear.In this study,we screened peanut varieties that were resistant and susceptible to A.flavus and evaluated their nodulation ability and growth performance after ARC microbial inoculants treatment in the field.The results demonstrated that the nodule number and nitrogenase activity of both varieties significantly increased after ARC microbial inoculants treatment,with the highly susceptible variety AH24 showing a greater increase.For photosynthetic parameters,both varieties also increased after ARC microbial inoculants treatment,but the increase was greater in the moderately resistant variety AH1 than in the highly susceptible variety AH24.Finally,we found that the yield and yield-related traits of the moderately resistant variety AH1 were better than those of the highly susceptible variety AH24.After ARC microbial inoculants treatment,the yield traits of both peanut varieties still increased significantly,but the degree of increase of the moderately resistant variety AH1 was smaller than that of the highly susceptible variety AH24.In addition,the abundance of A.flavus in the rhizosphere soil of the two varieties significantly decreased after ARC microbial inoculants treatment,with no significant difference between the varieties.These results indicated that ARC microbial inoculants exert differential effects on the nodulation and growth of different resistant peanut varieties and have a better effect on highly susceptible varieties.These results provide a solid theoretical basis for the efficient use of ARC microbial inoculants in the field of peanuts in the future.
基金supported by the National Key Research and Development Project of China(2021YFD1600101)the earmarked fund for China Agriculture research system(CARS-12 and CARS-13)the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2021-OCRI).
文摘As important supplementary to major edible oils, comparative chemical advantages of minor edible oils decidetheir development and usage. In this study, chemical composition of 13 kinds of specific edible vegetable oilswere investigated. The comparative advantages of chemical compositions of these edible oils were obtained asfollows: (1) camellia, tiger nut and almond oil were rich in oleic acid, the contents of which accounted for79.43%, 69.16% and 66.26%, respectively;(2) safflower oil contained the highest content of linoleic acid(76.69%), followed by grape seed (66.85%) and walnut oil (57.30%);(3) perilla seed, siritch, peony seed andherbaceous peony seed oil were rich in α-linolenic acid (59.61%, 43.74%, 40.83% and 30.84%, respectively);(4)the total phytosterol contents of these oils ranged from 91.46 mg/100 g (camellia oil) to 506.46 mg/100 g (siritchoil);and (5) The best source of tocopherols was sacha inchi oil (122.74 mg/100 g), followed by perilla seed oil(55.89 mg/100 g), peony seed oil (53.73 mg/100 g) and herbaceous peony seed oil (47.17 mg/100 g). Thecomparative advantages of these specific edible oils indicated that they possess the high potential nutritionalvalues and health care functions.
基金supported by National Key R&D Program of China (2016YFF0201803)the National Major Project for Agro-product Quality & Safety Risk Assessment(GJFP2017001,GJFP2017015-02)+1 种基金the earmarked fund for China Agriculture Research System (CARS-13-10B)Fundamental Research Funds for Central Non-profit Scientific Institution (1610172017008)
文摘To evaluate antibacterial activities of edible vegetable oils in China for healthy consumption, we investigated the antibacterial activities of extracts from 6 edible vegetable oils and rapeseed oils at different refining levels on 4 bactria (Staphylococcus aureus,Bacillus subtilis, Escherichia coli and Pseudomonas strains) by disc diffusion test. Results showed that camellia oil had considerable antibacterial activity against E. coli with antibacterial rate of 108.3%. Perilla oil, sunflower seed oil, and flaxseed oil showed the highest antibacterial activities against B. subtilis with the antibacterial rates of 108.3%, 102.8%and94.4%respectively. Moreover, the extracts of rapeseed oils obtained by different processing technologies showed significantly different antibacterial effects. Two crude oils and grade4 rapeseed oil showed antibacterial rates of more than 40%, whereas grade 1 and 2 rapeseed oils showed antibacterial rates of less than 20%. Therefore, it was recommended to use rapeseed oil rather than refined rapeseed oil because of higher antibacterial activities. It was suggested that proper refining processes should be taken to obtain the edible oils with higher antibacterial activities.
基金supported by National Science and Technology Major Project of China(2017YFC1601700)National Nature Foundation Committee of P.R.China(Grants No.31871886)+1 种基金National Major Project for Agro-product Quality&Safety Risk Assessment(GJFP2018001,GJFP2018015-04)FundamentalResearch Funds for Central Non-profit Scientific Institution(1610172018002,1610172018015)
文摘In this study, anthocyanin and fatty acid compositions between black and ordinary peanuts were compared. Results showed that black peanuts contained 93.90±18.36 μg/g of cyanidin, which was significantly higher than that of ordinary peanuts. In addition, although both of black and ordinary peanuts shared some specific fatty acids, 4 fatty acids in black peanuts were significantly different from those in ordinary peanuts, which could be used as markers to distinguish black peanut from ordinary ones. This study might serve as a reference for authenticity identification and further nutritional researches on black peanut.
基金This study was supported and funded by the National Key Research and Development Program of China(2021YFD1400900)the National Natural Science Foundation of China(31971665).
文摘Monochamus alternatus is the primary carrier of pine wood nematodes,which pose a serious threat to Pinus spp.in many countries.Newly emerging M.alternatus adults feed on heathy host pines,while matured adults transfer to stressed host pines for mating and oviposition.Several odorant-binding proteins(OBPs)of M.alternatus have been proved to aid in the complex process of host location.To clarify the corresponding relations between OBPs and pine volatiles,more OBPs need to be studied.In this research,MaltOBP19 showed a specific expression in the antennae and mouthparts of M.alternatus,and it was marked in 4 types of antenna sensilla by immunolocalization.Fluorescence binding assays demonstrated the high binding affinity of MaltOBP19 with camphene and myrcene in vitro.In Y-tube olfactory experiments,M.alternatus adults were attracted by camphene and RNAi of OBP19 via microinjection significantly decreased their attraction index.Myrcene induced phobotaxis,but RNAi had no significant effect on this behavior.Further,we found that ingesting dsOBP19 produced by a bacteria-expressed system with a newly constructed vector could lead to the knockdown of MaltOBP19.These results suggest that MaltOBP19 may play a role in the process of host conversion via the recognition of camphene,which has been identified to be strongly released in stressed host pines.In addition,it is proved that knockdown of OBP can be achieved by oral administration of bacteria-expressed double-stranded RNA in M.alternatus adults,providing a new perspective in the control of M.alternatus.
基金This work was supported by the National Natural Science Foundation of China(No.31972110).
文摘Encapsulation of phytosterols in nanostructured lipid carriers(NLCs)is an effective approach to improve their physicochemical properties,but the interaction of phytosterols in NLCs with cholesterol can be affected by lipid matrices.The objective of this study was to screen a desired phytosterols-loaded NLC(PS-NLC)formulation to reduce the absorption of intestinal cholesterol.A series of formulations were prepared using amphipathic glycerol monostearate(GMS)as the solid lipid fraction to increase lipid compatibity with phytosterols.All formulations showed a lowering effect on cholesterol bioaccessibility through in vitro intestinal digestion.And the PS-NLCs with medium chain triglycerides(MCT)as the liquid lipid showed significantly higher inhibition of cholesterol bioaccessibility(accessible fraction for intestinal absorption)than the formulations with long chain triglycerides(LCT),which could due to the solubilization of cholesterol by hydrolysis products of LCT,suggesting a notable impact of lipid type on cholesterol bioaccessibility.Furthermore,the analysis of distribution of phytosterols and cholesterol revealed a cocrystallization mechanism occurred during in vitro digestion.This study provides a certain reference for designing phytosterol-ingredient agents with a better hypocholesterolemic effect in water-dispersible functional foods.
