The farnesoid X receptor(FXR)plays a crucial role in alcohol-induced gut-liver axis injury.This study was designed to evaluate the therapeutic potential of Achyranthes bidentata polysaccharides(ABPs)in treating alcoho...The farnesoid X receptor(FXR)plays a crucial role in alcohol-induced gut-liver axis injury.This study was designed to evaluate the therapeutic potential of Achyranthes bidentata polysaccharides(ABPs)in treating alcohol-induced gut and liver injury in mice and to determine whether ABPs exert effects via FXR.Initially,the biological safety of ABPs in mice was confirmed by assessing serum levels of alanine aminotransferase(ALT)and aspartate aminotransferase(AST),as well as through hematoxylin and eosin staining of organ tissues.Subsequently,alcohol-induced liver and gut injury was established in mice using a liquid alcohol diet,followed by treatment with ABPs.The results demonstrated that oral administration of ABPs significantly reduced serum levels of ALT,AST,and total bile acids.Furthermore,ABPs treatment attenuated inflammatory cell infiltration and lipid droplet deposition in liver tissues,thereby ameliorating alcohol-induced liver injury.Additionally,ABPs modulated the FXR-SHP-CYP7A1 signaling pathway in the liver.ABPs also significantly repaired ileum injury in alcohol-induced liver disease mice and regulated FXR-FGF15 transcript levels in the ileum.Further,we validated the ability of ABPs restore the damaged intestinal barrier using intestinal epithelial cells.Collectively,our research revealed that oral administration of with ABPs exert a protective effect against alcohol-induced liver and ileal injury,primarily through the modulation of the FXR and associated signaling pathways within the gutliver axis.展开更多
基金supported by the Foundation of Sichuan Science and Technology Program(24NSFSC1611)the Third People’s Hospital of Chengdu Clinical Research Program(CSY-YN-01-2023-065 and CSY-YN-03-2024-015)Chengdu Medical Research Project Foundation(2022284).
文摘The farnesoid X receptor(FXR)plays a crucial role in alcohol-induced gut-liver axis injury.This study was designed to evaluate the therapeutic potential of Achyranthes bidentata polysaccharides(ABPs)in treating alcohol-induced gut and liver injury in mice and to determine whether ABPs exert effects via FXR.Initially,the biological safety of ABPs in mice was confirmed by assessing serum levels of alanine aminotransferase(ALT)and aspartate aminotransferase(AST),as well as through hematoxylin and eosin staining of organ tissues.Subsequently,alcohol-induced liver and gut injury was established in mice using a liquid alcohol diet,followed by treatment with ABPs.The results demonstrated that oral administration of ABPs significantly reduced serum levels of ALT,AST,and total bile acids.Furthermore,ABPs treatment attenuated inflammatory cell infiltration and lipid droplet deposition in liver tissues,thereby ameliorating alcohol-induced liver injury.Additionally,ABPs modulated the FXR-SHP-CYP7A1 signaling pathway in the liver.ABPs also significantly repaired ileum injury in alcohol-induced liver disease mice and regulated FXR-FGF15 transcript levels in the ileum.Further,we validated the ability of ABPs restore the damaged intestinal barrier using intestinal epithelial cells.Collectively,our research revealed that oral administration of with ABPs exert a protective effect against alcohol-induced liver and ileal injury,primarily through the modulation of the FXR and associated signaling pathways within the gutliver axis.
