Mpox is a zoonotic disease caused by the monkeypox virus(MPxV).Diagnosing and treating the disease has become a global health concern requiring close attention to its spread to non-endemic regions.Clinical diag-nosis ...Mpox is a zoonotic disease caused by the monkeypox virus(MPxV).Diagnosing and treating the disease has become a global health concern requiring close attention to its spread to non-endemic regions.Clinical diag-nosis is based on laboratory test results.Conventional detection techniques include real-time quantitative poly-merase chain reaction(qPCR),genome sequencing,antigen and antibody identification,and virus isolation.Nevertheless,these methods fall short of rapidly and efficiently identifying MPxV,as they require specialized training,specific laboratory environments,and professional-grade equipment.Emerging technologies offer complementary advantages to meet diverse diagnostic needs,including various point-of-care testing(POcT)approaches and integrating biosensors with rapid detection techniques.This review discusses prospective future research avenues for MpxV detection,examining the advances and challenges of various detection tech-niques which may contribute to the ongoing elimination of mpox human-to-human transmission and serves as a reference for developing effective prevention and control strategies.展开更多
基金financial support was received for the research,authorship,and/or publication of this articlefunded by Nanjing Bioengineering(Gene)Technology Center for Medicines(2024YQFH01)。
文摘Mpox is a zoonotic disease caused by the monkeypox virus(MPxV).Diagnosing and treating the disease has become a global health concern requiring close attention to its spread to non-endemic regions.Clinical diag-nosis is based on laboratory test results.Conventional detection techniques include real-time quantitative poly-merase chain reaction(qPCR),genome sequencing,antigen and antibody identification,and virus isolation.Nevertheless,these methods fall short of rapidly and efficiently identifying MPxV,as they require specialized training,specific laboratory environments,and professional-grade equipment.Emerging technologies offer complementary advantages to meet diverse diagnostic needs,including various point-of-care testing(POcT)approaches and integrating biosensors with rapid detection techniques.This review discusses prospective future research avenues for MpxV detection,examining the advances and challenges of various detection tech-niques which may contribute to the ongoing elimination of mpox human-to-human transmission and serves as a reference for developing effective prevention and control strategies.
