Pharmacy is an important subfield of the medical sciences,and its development is inseparable from pharmacy education.Pharmacy education outputs indispensable professionals and expertise for the healthcare industry,pro...Pharmacy is an important subfield of the medical sciences,and its development is inseparable from pharmacy education.Pharmacy education outputs indispensable professionals and expertise for the healthcare industry,promotes the development of pharmacy skills,and supports the development of the pharmaceutical and health sectors.To this end,many insightful studies have been carried out to promote the development and progress of pharmacy education.These studies have been published in high-quality pharmacy education journals indexed in the Web of Science Core Collection(WoSCC),and the two journals with the largest number of published articles are the American Journal of Pharmaceutical Education and Currents in Pharmacy Teaching and Learning.The 200 most-cited papers historically in these two journals have a significant impact on the research paradigm and future directions in the field of pharmacy education.Therefore,this paper summarizes the knowledge domains of these 200 papers and uses the scientometric method to study their basic information,authorship distribution,citation performance,publishing paradigm and hotspot mining.The era of Holistic Integrative Pharmacy(HIP),in which the focus has shifted from isolated pharmacological interventions to more collaborative,patient-centered,and interdisciplinary approaches,underscores the importance of evolving educational methodologies.In this context,understanding the scientometric trends in pharmacy education becomes even more crucial.This study aims to explore the current development status of pharmacy education,develop more innovative and effective educational models,and promote the widespread application of HIP in pharmacy education.The goal is to enhance the overall quality of pharmacy education and provide guidance and insights for the future development of pharmacy education.展开更多
Purpose： This study aims at identifying potential industry-university-research collaboration（IURC） partners effectively and analyzes the conditions and dynamics in the IURC process based on innovation chain theory....Purpose： This study aims at identifying potential industry-university-research collaboration（IURC） partners effectively and analyzes the conditions and dynamics in the IURC process based on innovation chain theory.Design/methodology/approach： The method utilizes multisource data, combining bibliometric and econometrics analyses to capture the core network of the existing collaboration networks and institution competitiveness in the innovation chain. Furthermore, a new identification method is constructed that takes into account the law of scientific research cooperation and economic factors.Findings： Empirical analysis of the genetic engineering vaccine field shows that through the distribution characteristics of creative technologies from different institutions, the analysis based on the innovation chain can identify the more complementary capacities among organizations.Research limitations： In this study, the overall approach is shaped by the theoretical concept of an innovation chain, a linear innovation model with specific types or stages of innovation activities in each phase of the chain, and may, thus, overlook important feedback mechanisms in the innovation process.Practical implications： Industry-university-research institution collaborations are extremely important in promoting the dissemination of innovative knowledge, enhancing the quality of innovation products, and facilitating the transformation of scientific achievements.Originality/value： Compared to previous studies, this study emulates the real conditions of IURC. Thus, the rule of technological innovation can be better revealed, the potential partners of IURC can be identified more readily, and the conclusion has more value.展开更多
Highly flame-retardant bio-based composites were prepared in this study.Firstly,glucose-citric acid(GC)resin was synthesized through the interaction of glucose and citric acid derived from agricultural and forestry so...Highly flame-retardant bio-based composites were prepared in this study.Firstly,glucose-citric acid(GC)resin was synthesized through the interaction of glucose and citric acid derived from agricultural and forestry sources.Polyvinyl alcohol(PVA)served as a toughening agent,whereas walnut shell powder(WSP)functioned as a filler in the formulation of a thermosetting bio-based GC-PVA-WSP(GCPW)composite with GC resin.The findings demonstrated that boric acid increased the limited oxygen index(LOI)value of GCPW to 33%,while simultaneously diminishing its total smoke production(TSP)by 99.9%,and achieving a flame retardant index(FRI)of 5.04.In addition,the incorporation of WSP enhanced the compressive strength of the GCPW composite to 9.15 MPa.Concurrently,the GCPW composite demonstrates excellent hydrophobic properties,with a thermal conductivity as low as 0.086 W/m·K.展开更多
Objective To develop a multimodal imaging atlas of a rat brain-computer interface(BCI)that incorporates brain,arterial,bone tissue and a BCI device using mixed reality(MR)for three-dimensional(3D)visualization.Methods...Objective To develop a multimodal imaging atlas of a rat brain-computer interface(BCI)that incorporates brain,arterial,bone tissue and a BCI device using mixed reality(MR)for three-dimensional(3D)visualization.Methods An invasive BCI was implanted in the left visual cortex of 4-week-old Sprague–Dawley rats.Multimodal imaging techniques,including micro-CT and 9.0 T MRI,were used to acquire images of the rat cranial bone structure,vascular distribution,brain tissue functional zones,and BCI device before and after implantation.Using 3D-slicer software,the images were fused through spatial transformations,followed by image segmentation and 3D model reconstruction.The HoloLens platform was employed for MR visualization.Results This study constructed a multimodal imaging atlas for rats that included the skull,brain tissue,arterial tissue,and BCI device coupled with MR technology to create an interactive 3D anatomical model.Conclusions This multimodal 3D atlas provides an objective and stable reference for exploring complex relationships between brain tissue structure and function,enhancing the understanding of the operational principles of BCIs.This is the first multimodal 3D imaging atlas related to a BCI created using Sprague–Dawley rats.展开更多
BACKGROUND Esophagogastric variceal bleeding(EGVB)is a serious complication of patients with decompensated cirrhosis and is associated with high mortality and morbidity.Early diagnosis and screening of cirrhotic patie...BACKGROUND Esophagogastric variceal bleeding(EGVB)is a serious complication of patients with decompensated cirrhosis and is associated with high mortality and morbidity.Early diagnosis and screening of cirrhotic patients at risk for EGVB is crucial.Currently,there is a lack of noninvasive predictive models widely available in clinical practice.AIM To develop a nomogram based on clinical variables and radiomics to facilitate the noninvasive prediction of EGVB in cirrhotic patients.METHODS A total of 211 cirrhotic patients hospitalized between September 2017 and December 2021 were included in this retrospective study.Patients were divided into training(n=149)and validation(n=62)groups at a 7:3 ratio.Participants underwent three-phase computed tomography(CT)scans before endoscopy,and radiomic features were extracted from portal venous phase CT images.The independent sample t-test and least absolute shrinkage and selection operator logistic regression were used to screen out the best features and establish a radiomics signature(RadScore).Univariate and multivariate analyses were performed to determine the independent predictors of EGVB in clinical settings.A noninvasive predictive nomogram for the risk of EGVB was built using independent clinical predictors and RadScore.Receiver operating characteristic,calibration,clinical decision,and clinical impact curves were applied to evaluate the model’s performance.RESULTS Albumin(P=0.001),fibrinogen(P=0.001),portal vein thrombosis(P=0.002),aspartate aminotransferase(P=0.001),and spleen thickness(P=0.025)were selected as independent clinical predictors of EGVB.RadScore,constructed with five CT features of the liver region and three of the spleen regions,performed well in training(area under the receiver operating characteristic curve(AUC)=0.817)as well as in validation(AUC=0.741)cohorts.There was excellent predictive performance in both the training and validation cohorts for the clinical-radiomics model(AUC=0.925 and 0.912,respectively).Compared with the existing noninvasive models such as ratio of aspartate aminotransferase to platelets and Fibrosis-4 scores,our combined model had better predictive accuracy with the Delong's test less than 0.05.The Nomogram had a good fit in the calibration curve(P>0.05),and the clinical decision curve further supported its clinical utility.CONCLUSION We designed and validated a clinical-radiomics nomogram able to noninvasively predict whether cirrhotic patients will develop EGVB,thus facilitating early diagnosis and treatment.展开更多
Chemodynamic therapy(CDT),defined as an in situ oxidative stress response catalyzed by the Fenton or Fenton-like reactions to generate cytotoxic hydroxyl radicals(•OH)at tumor sites,exhibits conspicuous inhibition of ...Chemodynamic therapy(CDT),defined as an in situ oxidative stress response catalyzed by the Fenton or Fenton-like reactions to generate cytotoxic hydroxyl radicals(•OH)at tumor sites,exhibits conspicuous inhibition of tumor growth.It has attracted extensive attention for its outstanding edge in effectiveness,lower systemic toxicity and side effects,sustainability,low cost and convenience.However,the inconfor-mity of harsh Fenton reaction conditions and tumor microenvironment hamper its further development,based on which,numerous researchers have made efforts in further improving the efficiency of CDT.In this review,we expounded antitumor capacity of CDT in mechanism,together with its limitation,and then summarized and came up with several strategies to enhance CDT involved tumor therapy strategies by 1)improving catalytic efficiency;2)increasing hydrogen peroxide levels at tumor sites;3)reducing glutathione levels at tumor sites;4)applying external energy intervention;5)amplifying the distribu-tion of hydroxyl radicals at tumor sites;and 6)combination therapy.Eventually,the perspectives and challenges of CDT are further discussed to encourage more in-depth studies and rational reflections.展开更多
Single-pass compression tests of an aluminaforming austenite(AFA) alloy(Fe–20Cr–30Ni–0.6Nb–2Al–Mo) were performed using a Gleeble-3500 thermal–mechanical simulator. By combining techniques of electron back-scatt...Single-pass compression tests of an aluminaforming austenite(AFA) alloy(Fe–20Cr–30Ni–0.6Nb–2Al–Mo) were performed using a Gleeble-3500 thermal–mechanical simulator. By combining techniques of electron back-scattered diffraction(EBSD) and transmission electron microscopy(TEM), the dynamic recrystallization(DRX) behavior of the alloy at temperatures of 950–1100 ℃ and strain rates of 0.01–1.00 s^(-1) was investigated. The regression method was adopted to determine the thermal deformation activation energy and apparent stress index and to construct a thermal deformation constitutive model. Results reveal that the flow stress is strongly dependent on temperature and strain rate and it increases with temperature decreasing and strain rate increasing. The DRX phenomenon occurs more easily at comparably higher deformation temperatures and lower strain rates. Based on the method for solving the inflection point via cubic polynomial fitting of strain hardening rate(h) versus strain(e) curves, the ratio of critical strain(ec) to peak strain(ep) during DRX was precisely predicted. The nucleation mechanisms of DRX during thermal deformation mainly include the strain-induced grain boundary(GB)migration, grain fragmentation, and subgrain coalescence.展开更多
We report the properties of more than 800 bursts detected from the repeating fast radio burst(FRB)source FRB20201124A with the Five-hundred-meter Aperture Spherical radio Telescope(FAST)during an extremely active epis...We report the properties of more than 800 bursts detected from the repeating fast radio burst(FRB)source FRB20201124A with the Five-hundred-meter Aperture Spherical radio Telescope(FAST)during an extremely active episode on UTC 2021 September 25–28 in a series of four papers.In this second paper of the series,we study the energy distribution of 881 bursts(defined as significant signals separated by dips down to the noise level)detected in the first four days of our 19 hr observational campaign spanning 17 days.The event rate initially increased exponentially but the source activity stopped within 24 hr after the 4th day.The detection of 542 bursts in one hour during the fourth day marked the highest event rate detected from one single FRB source so far.The bursts have complex structures in the time-frequency space.We find a double-peak distribution of the waiting time,which can be modeled with two log-normal functions peaking at 51.22 ms and 10.05 s,respectively.Compared with the emission from a previous active episode of the source detected with FAST,the second distribution peak time is smaller,suggesting that this peak is defined by the activity level of the source.We calculate the isotropic energy of the bursts using both a partial bandwidth and a full bandwidth and find that the energy distribution is not significantly changed.We find that an exponentially connected broken-power law function can fit the cumulative burst energy distribution well,with the lower and higher-energy indices being-1.22±0.01 and-4.27±0.23,respectively.Assuming a radio radiative efficiency ofη_(r)=10^(-4),the total isotropic energy of the bursts released during the four days when the source was active is already 3.9×10^(46)erg,exceeding~23%of the available magnetar dipolar magnetic energy.This challenges the magnetar models which invoke an inefficient radio emission(e.g.,synchrotron maser models).展开更多
We report the properties of more than 800 bursts detected from the repeating fast radio burst(FRB)source FRB 20201124A with the Five-hundred-meter Aperture Spherical radio Telescope during an extremely active episode ...We report the properties of more than 800 bursts detected from the repeating fast radio burst(FRB)source FRB 20201124A with the Five-hundred-meter Aperture Spherical radio Telescope during an extremely active episode on UTC 2021 September 25th-28th in a series of four papers.In this fourth paper of the series,we present a systematic search of the spin period and linear acceleration of the source object from both 996 individual pulse peaks and the dedispersed time series.No credible spin period was found from this data set.We rule out the presence of significant periodicity in the range between 1 ms and 100 s with a pulse duty cycle<0.49±0.08(when the profile is defined by a von-Mises function,not a boxcar function)and linear acceleration up to 300 m s^(-2)in each of the four one-hour observing sessions,and up to 0.6 m s^(-2)in all 4 days.These searches contest theoretical scenarios involving a 1 ms–100 s isolated magnetar/pulsar with surface magnetic field<10^(15)G and a small duty cycle(such as in a polar-cap emission mode)or a pulsar with a companion star or black hole up to 100 M_(⊙)and P_(b)>10 hr.We also perform a periodicity search of the fine structures and identify 53 unrelated millisecond-timescale“periods”in multicomponents with the highest significance of 3.9σ.The“periods”recovered from the fine structures are neither consistent nor harmonically related.Thus they are not likely to come from a spin period.We caution against claiming spin periodicity with significance below~4σwith multi-components from one-off FRBs.We discuss the implications of our results and the possible connections between FRB multi-components and pulsar microstructures.展开更多
The aim of this study is to evaluate the uncertainty of 2πα and 2πβ surface emission rates using the windowless multiwire proportional counter method.This study used the Monte Carlo method (MCM) to validate the co...The aim of this study is to evaluate the uncertainty of 2πα and 2πβ surface emission rates using the windowless multiwire proportional counter method.This study used the Monte Carlo method (MCM) to validate the conventional Guide to the Expression of Uncertainty in Measurement (GUM) method.A dead time measurement model for the two-source method was established based on the characteristics of a single-channel measurement system,and the voltage threshold correction factor measurement function was indirectly obtained by fitting the threshold correction curve.The uncertainty in the surface emission rate was calculated using the GUM method and the law of propagation of uncertainty.The MCM provided clear definitions for each input quantity and its uncertainty distribution,and the simulation training was realized with a complete and complex mathematical model.The results of the surface emission rate uncertainty evaluation for four radioactive plane sources using both methods showed the uncertainty’s consistency E_(n)<0.070 for the comparison of each source,and the uncertainty results of the GUM were all lower than those of the MCM.However,the MCM has a more objective evaluation process and can serve as a validation tool for GUM results.展开更多
In the present work, the orientation characteristics of residual grains during hot deformation of an age-hardening Ni-Fe-Cr alloy(Alloy 925) at different conditions were systematically analyzed through high-resolution...In the present work, the orientation characteristics of residual grains during hot deformation of an age-hardening Ni-Fe-Cr alloy(Alloy 925) at different conditions were systematically analyzed through high-resolution electron back-scatter diffraction. Based on the measurement of the kernel average misorientation, the density of geometrical necessary dislocations(GNDs) was further calculated. The orientation-dependent deformation mechanism of the residual grains was also discussed using Schmid factor difference ratio(SFDR) analysis. The results show that the deformed microstructure features typical "necklace" structures. Many distorted twin boundaries can be observed within the residual grains at 950 ℃. When the deformation temperature increases to 1150 ℃, the volume fraction of dynamic recrystallization(DRX) increases, leading to the extensive formation of primary Σ3 twin boundaries. Additionally, the GNDs are widely distributed in the residual grains, while they are rare for the recrystallized grains. The maximum GND density value can be obtained at the interface of "soft–hard" grains. The GND density also increases at higher strain rates, and the number of DRX grains significantly affects the distribution of the GND density. Moreover, based on the calculations and SFDR analysis, it can be summarized that the {100} and {111} grains are prone to deform in the uniserial slip mode and the multiple slip mode, respectively.展开更多
The hot deformation behavior of 316H stainless steel used in the 4th-generation nuclear systems was investigated by thermal compression tests at 1000–1150 C and 0.01–10 s^(-1).It was found that true stress firstly i...The hot deformation behavior of 316H stainless steel used in the 4th-generation nuclear systems was investigated by thermal compression tests at 1000–1150 C and 0.01–10 s^(-1).It was found that true stress firstly increased and then decreased with the increasing strain rate with a threshold of 1 s^(-1).Electron backscatter diffraction was used to analyze the microstructure evolution.Discontinuous dynamic recrystallization(DDRX)was the dominant dynamic recrystallization(DRX)mechanism,while continuous dynamic recrystallization(CDRX)was the supplementary one.DDRX happened before CDRX and provided additional nucleation sites for the latter.Twin grain boundaries(R3)appeared in DRX grains due to growth accidents.As the length fraction of R3 increased,the coincidence site lattice(CSL)boundary transition began to occur,forming R9 and R27.After the occurrence of full DRX,the growth and annexation of DRX grains were easy to be promoted,in which progress both equiaxed grains and CSL boundaries disappeared.The ideal deformation microstructure with fine and uniform DRX grains,which was accompanied by a high length fraction of CSL boundaries,appeared at 1000℃–0.01 s^(-1),1050℃–0.01–0.1 s^(-1),1100℃–0.1–1 s^(-1) and 1150℃–1–10 s^(-1).That is,the deformation conditions mentioned above were the preferable thermal forming parameters for 316H stainless steel in actual productions.展开更多
Understanding the immunological characteristics of monocytes-including the characteristics associated with fibrosis-in severe coronavirus disease 2019(COVID-19)is crucial for understanding the pathogenic mechanism of ...Understanding the immunological characteristics of monocytes-including the characteristics associated with fibrosis-in severe coronavirus disease 2019(COVID-19)is crucial for understanding the pathogenic mechanism of the disease and preventing disease severity.In this study,we performed single-cell transcriptomic sequencing of peripheral blood samples collected from six healthy controls and 14 COVID-19samples including severe,moderate,and convalescent samples from three severely/critically ill and four moderately ill patients.We found that the monocytes were strongly remodeled in the severely/critically ill patients with COVID-19,with an increased proportion of monocytes and seriously reduced diversity.In addition,we discovered two novel severe-disease-specific monocyte subsets:Mono 0 and Mono 5.These subsets expressed amphiregulin(AREG),epiregulin(EREG),and cytokine interleukin-18(IL-18)gene,exhibited an enriched erythroblastic leukemia viral oncogene homolog(ErbB)signaling pathway,and appeared to exhibit pro-fibrogenic and pro-inflammation characteristics.We also found metabolic changes in Mono 0 and Mono 5,including increased glycolysis/gluconeogenesis and an increased hypoxia inducible factor-1(HIF-1)signaling pathway.Notably,one pre-severe sample displayed a monocyte atlas similar to that of the severe/critical samples.In conclusion,our study discovered two novel severedisease-specific monocyte subsets as potential predictors and therapeutic targets for severe COVID-19.Overall,this study provides potential predictors for severe disease and therapeutic targets for COVID-19 and thus provides a resource for further studies on COVID-19.展开更多
Objective This study aimed to evaluate and compare the effectiveness of knowledge base-optimized and unoptimized large language models(LLMs)in the field of orthopedics to explore optimization strategies for the applic...Objective This study aimed to evaluate and compare the effectiveness of knowledge base-optimized and unoptimized large language models(LLMs)in the field of orthopedics to explore optimization strategies for the application of LLMs in specific fields.Methods This research constructed a specialized knowledge base using clinical guidelines from the American Academy of Orthopaedic Surgeons(AAOS)and authoritative orthopedic publications.A total of 30 orthopedic-related questions covering aspects such as anatomical knowledge,disease diagnosis,fracture classification,treatment options,and surgical techniques were input into both the knowledge base-optimized and unoptimized versions of the GPT-4,ChatGLM,and Spark LLM,with their generated responses recorded.The overall quality,accuracy,and comprehensiveness of these responses were evaluated by 3 experienced orthopedic surgeons.Results Compared with their unoptimized LLMs,the optimized version of GPT-4 showed improvements of 15.3%in overall quality,12.5%in accuracy,and 12.8%in comprehensiveness;ChatGLM showed improvements of 24.8%,16.1%,and 19.6%,respectively;and Spark LLM showed improvements of 6.5%,14.5%,and 24.7%,respectively.Conclusion The optimization of knowledge bases significantly enhances the quality,accuracy,and comprehensiveness of the responses provided by the 3 models in the orthopedic field.Therefore,knowledge base optimization is an effective method for improving the performance of LLMs in specific fields.展开更多
The effect of temperature on the tensile properties and deformation mechanism of GH4169 alloy has been systematically studied over a wide range of room temperature(RT)to 1000℃.The results indicate that the stress–st...The effect of temperature on the tensile properties and deformation mechanism of GH4169 alloy has been systematically studied over a wide range of room temperature(RT)to 1000℃.The results indicate that the stress–strain curve of the alloy shows serrations at 200–600℃,and the character of the serrations changes from type A to type B and then to type C at different temperatures.The ultimate tensile strength of the alloy decreases gradually from RT to 600℃.The yield strength decreases slowly from RT to 700℃ but decreases rapidly above 800℃.Transmission electron microscopy analysis relieves that the primary deformation mechanism of the alloy below 500℃ is Orowan bypass mechanism.At temperatures between 600 and 700℃,the coordinated deformation of twins and cross-slip of dislocations are activated.The transformation of\upgamma^{\prime\prime}phase toδphase above 650℃ will decrease the strength.The primary deformation mechanism above 800℃ transforms into the repeated shearing of\upgamma^{\prime\prime}by dislocations to form multiple stacking faults.Recrystallized grains were observed above 800℃,and continuous dynamic recrystallization and discontinuous dynamic recrystallization were observed.The stress concentration caused by Nb-rich carbides is the cause of intracrystalline crack nucleation.At 700℃,grain boundary crack sprouting is caused by the combined effect of slip band impact on grain boundaries and grain boundary dislocation plugging.The relationship between the serrated flow behavior and the deformation mechanism has been discussed based on the experimental results.展开更多
Castor(Ricinus communis L.)is one of the top 10 oil crops in the world,and inflorescence is a trait that directly affects its yield.Phospholipase C(PLCs)is involved in many plant activities and metabolic processes.To ...Castor(Ricinus communis L.)is one of the top 10 oil crops in the world,and inflorescence is a trait that directly affects its yield.Phospholipase C(PLCs)is involved in many plant activities and metabolic processes.To study the functions of PLC family genes in the regulation of the inflorescence development of the female line of Lm-type castor aLmAB2,we determined the expression levels of six PLC family genes of three types of inflorescences of aLmAB2(isofemale line,female line,bisexual line)at different developmental stages.The results showed that the 6 genes of the castor PLC family had relative expression levels at different developmental stages of the three types of inflorescences.The subcellular location of all six protein products was the cell membrane.The six genes were heterologously overexpressed in Arabidopsis thaliana to obtain the T3 generation-resistant Arabidopsis thaliana plants.The results showed that the overexpression of six genes significantly promoted the maturation of Arabidopsis thaliana,the growth of lateral moss,and the development of flowers and pods,but the development of basal leaves and stem leaves of Arabidopsis thaliana was significantly inhibited.According to homology analysis,it is speculated that PLC2,PLC2M,PLC2N,PLC4,PLC4X2,and PLC6 genes have the same regulatory function.展开更多
Castor(Ricinus communis L.)is one of ten oil crops in the world and has complex inflorescence styles.Generally,castor has three inflorescence types:single female inflorescence(SiFF),standard female inflorescence(StFF)...Castor(Ricinus communis L.)is one of ten oil crops in the world and has complex inflorescence styles.Generally,castor has three inflorescence types:single female inflorescence(SiFF),standard female inflorescence(StFF)and bisexual inflorescence(BF).StFF is realized as a restorer line and as a maintainer line,which was applied to castor hybrid breeding.However,the developmental mechanism of the three inflorescences is not clear.Therefore,we used proteomic techniques to analyze different inflorescence styles.A total of 72 diferentially abundant protein species(DAPs)were detected.These DAPs are primarily involved in carbon and energy metabolism and carbon fixation in the photosynthetic organism pathway.The results showed that DAPs are involved in photosynthesis to control the distribution of imported carbohydrates and exported photoassimilates and thus affect the inflorescence development of castor.In addition,these DAPs are also involved in cysteine and methionine metabolism.Quantitative real-time PCR(qRT-PCR)results demonstrated that the proteomics data collected in this study were reliable.Our findings indicate that the carbon cycle and amino acid metabolism influence the inflorescence development of castor.展开更多
基金National Natural Science Foundation of China(grant No.82373800)Guangzhou Science and Technology Plan Project(grant No.202201010589).
文摘Pharmacy is an important subfield of the medical sciences,and its development is inseparable from pharmacy education.Pharmacy education outputs indispensable professionals and expertise for the healthcare industry,promotes the development of pharmacy skills,and supports the development of the pharmaceutical and health sectors.To this end,many insightful studies have been carried out to promote the development and progress of pharmacy education.These studies have been published in high-quality pharmacy education journals indexed in the Web of Science Core Collection(WoSCC),and the two journals with the largest number of published articles are the American Journal of Pharmaceutical Education and Currents in Pharmacy Teaching and Learning.The 200 most-cited papers historically in these two journals have a significant impact on the research paradigm and future directions in the field of pharmacy education.Therefore,this paper summarizes the knowledge domains of these 200 papers and uses the scientometric method to study their basic information,authorship distribution,citation performance,publishing paradigm and hotspot mining.The era of Holistic Integrative Pharmacy(HIP),in which the focus has shifted from isolated pharmacological interventions to more collaborative,patient-centered,and interdisciplinary approaches,underscores the importance of evolving educational methodologies.In this context,understanding the scientometric trends in pharmacy education becomes even more crucial.This study aims to explore the current development status of pharmacy education,develop more innovative and effective educational models,and promote the widespread application of HIP in pharmacy education.The goal is to enhance the overall quality of pharmacy education and provide guidance and insights for the future development of pharmacy education.
基金funded by National Natural Science Foundation of China (Grant No. 71704170)the China Postdoctoral Science Foundation funded project (Grant No. 2016M590124)the Youth Innovation Promotion Association, CAS (Grant No. 2016159)
文摘Purpose： This study aims at identifying potential industry-university-research collaboration（IURC） partners effectively and analyzes the conditions and dynamics in the IURC process based on innovation chain theory.Design/methodology/approach： The method utilizes multisource data, combining bibliometric and econometrics analyses to capture the core network of the existing collaboration networks and institution competitiveness in the innovation chain. Furthermore, a new identification method is constructed that takes into account the law of scientific research cooperation and economic factors.Findings： Empirical analysis of the genetic engineering vaccine field shows that through the distribution characteristics of creative technologies from different institutions, the analysis based on the innovation chain can identify the more complementary capacities among organizations.Research limitations： In this study, the overall approach is shaped by the theoretical concept of an innovation chain, a linear innovation model with specific types or stages of innovation activities in each phase of the chain, and may, thus, overlook important feedback mechanisms in the innovation process.Practical implications： Industry-university-research institution collaborations are extremely important in promoting the dissemination of innovative knowledge, enhancing the quality of innovation products, and facilitating the transformation of scientific achievements.Originality/value： Compared to previous studies, this study emulates the real conditions of IURC. Thus, the rule of technological innovation can be better revealed, the potential partners of IURC can be identified more readily, and the conclusion has more value.
基金supported by the Natural Science Foundation of China(32460363)Yunnan Province Agricultural Joint Key Foundation(No.202401BD070001-029)+3 种基金Yunnan Agricultural Joint General Foundation(202101BD070001-105)the Yunnan Provincial Youth Top Talent Project(Grant No.YNWR-QNBJ-2020-166)the Foreign ExpertWorkstation(202305AF150006)the 111 Project(D21027).
文摘Highly flame-retardant bio-based composites were prepared in this study.Firstly,glucose-citric acid(GC)resin was synthesized through the interaction of glucose and citric acid derived from agricultural and forestry sources.Polyvinyl alcohol(PVA)served as a toughening agent,whereas walnut shell powder(WSP)functioned as a filler in the formulation of a thermosetting bio-based GC-PVA-WSP(GCPW)composite with GC resin.The findings demonstrated that boric acid increased the limited oxygen index(LOI)value of GCPW to 33%,while simultaneously diminishing its total smoke production(TSP)by 99.9%,and achieving a flame retardant index(FRI)of 5.04.In addition,the incorporation of WSP enhanced the compressive strength of the GCPW composite to 9.15 MPa.Concurrently,the GCPW composite demonstrates excellent hydrophobic properties,with a thermal conductivity as low as 0.086 W/m·K.
基金supported by the National Natural Science Foundation of China(No.82172524 and No.81974355)National Innovation Platform Development Program(No.2020021105012440),China+3 种基金Major Program of Hubei Province(No.2021BEA161),ChinaMajor Key Project of Hubei Province(No.JD2023BAA005),ChinaWuhan Union Hospital Free Innovation Preliminary Research Fund(No.2024XHYN047),ChinaJoint Funds for the Innovation of Science and Technology(No.2024Y9062),Fujian Province,China.
文摘Objective To develop a multimodal imaging atlas of a rat brain-computer interface(BCI)that incorporates brain,arterial,bone tissue and a BCI device using mixed reality(MR)for three-dimensional(3D)visualization.Methods An invasive BCI was implanted in the left visual cortex of 4-week-old Sprague–Dawley rats.Multimodal imaging techniques,including micro-CT and 9.0 T MRI,were used to acquire images of the rat cranial bone structure,vascular distribution,brain tissue functional zones,and BCI device before and after implantation.Using 3D-slicer software,the images were fused through spatial transformations,followed by image segmentation and 3D model reconstruction.The HoloLens platform was employed for MR visualization.Results This study constructed a multimodal imaging atlas for rats that included the skull,brain tissue,arterial tissue,and BCI device coupled with MR technology to create an interactive 3D anatomical model.Conclusions This multimodal 3D atlas provides an objective and stable reference for exploring complex relationships between brain tissue structure and function,enhancing the understanding of the operational principles of BCIs.This is the first multimodal 3D imaging atlas related to a BCI created using Sprague–Dawley rats.
基金The study was reviewed and approved by the Ethics Committee of the Second Affiliated Hospital of Chongqing Medical University(No.2022-149).
文摘BACKGROUND Esophagogastric variceal bleeding(EGVB)is a serious complication of patients with decompensated cirrhosis and is associated with high mortality and morbidity.Early diagnosis and screening of cirrhotic patients at risk for EGVB is crucial.Currently,there is a lack of noninvasive predictive models widely available in clinical practice.AIM To develop a nomogram based on clinical variables and radiomics to facilitate the noninvasive prediction of EGVB in cirrhotic patients.METHODS A total of 211 cirrhotic patients hospitalized between September 2017 and December 2021 were included in this retrospective study.Patients were divided into training(n=149)and validation(n=62)groups at a 7:3 ratio.Participants underwent three-phase computed tomography(CT)scans before endoscopy,and radiomic features were extracted from portal venous phase CT images.The independent sample t-test and least absolute shrinkage and selection operator logistic regression were used to screen out the best features and establish a radiomics signature(RadScore).Univariate and multivariate analyses were performed to determine the independent predictors of EGVB in clinical settings.A noninvasive predictive nomogram for the risk of EGVB was built using independent clinical predictors and RadScore.Receiver operating characteristic,calibration,clinical decision,and clinical impact curves were applied to evaluate the model’s performance.RESULTS Albumin(P=0.001),fibrinogen(P=0.001),portal vein thrombosis(P=0.002),aspartate aminotransferase(P=0.001),and spleen thickness(P=0.025)were selected as independent clinical predictors of EGVB.RadScore,constructed with five CT features of the liver region and three of the spleen regions,performed well in training(area under the receiver operating characteristic curve(AUC)=0.817)as well as in validation(AUC=0.741)cohorts.There was excellent predictive performance in both the training and validation cohorts for the clinical-radiomics model(AUC=0.925 and 0.912,respectively).Compared with the existing noninvasive models such as ratio of aspartate aminotransferase to platelets and Fibrosis-4 scores,our combined model had better predictive accuracy with the Delong's test less than 0.05.The Nomogram had a good fit in the calibration curve(P>0.05),and the clinical decision curve further supported its clinical utility.CONCLUSION We designed and validated a clinical-radiomics nomogram able to noninvasively predict whether cirrhotic patients will develop EGVB,thus facilitating early diagnosis and treatment.
基金supported by the National Natural Science Foundation of China(No.81822025)1.3.5 Project for Disciplines of Excellence,West China Hospital,Sichuan University(No.ZYYC08002).
文摘Chemodynamic therapy(CDT),defined as an in situ oxidative stress response catalyzed by the Fenton or Fenton-like reactions to generate cytotoxic hydroxyl radicals(•OH)at tumor sites,exhibits conspicuous inhibition of tumor growth.It has attracted extensive attention for its outstanding edge in effectiveness,lower systemic toxicity and side effects,sustainability,low cost and convenience.However,the inconfor-mity of harsh Fenton reaction conditions and tumor microenvironment hamper its further development,based on which,numerous researchers have made efforts in further improving the efficiency of CDT.In this review,we expounded antitumor capacity of CDT in mechanism,together with its limitation,and then summarized and came up with several strategies to enhance CDT involved tumor therapy strategies by 1)improving catalytic efficiency;2)increasing hydrogen peroxide levels at tumor sites;3)reducing glutathione levels at tumor sites;4)applying external energy intervention;5)amplifying the distribu-tion of hydroxyl radicals at tumor sites;and 6)combination therapy.Eventually,the perspectives and challenges of CDT are further discussed to encourage more in-depth studies and rational reflections.
基金financially supported by the National Natural Science Foundation of China (No. 2012AA03A501)the Ordinary University Graduate Student Scientific Research Innovation Projects by Jiangsu Province (No. KYLX-1027)
文摘Single-pass compression tests of an aluminaforming austenite(AFA) alloy(Fe–20Cr–30Ni–0.6Nb–2Al–Mo) were performed using a Gleeble-3500 thermal–mechanical simulator. By combining techniques of electron back-scattered diffraction(EBSD) and transmission electron microscopy(TEM), the dynamic recrystallization(DRX) behavior of the alloy at temperatures of 950–1100 ℃ and strain rates of 0.01–1.00 s^(-1) was investigated. The regression method was adopted to determine the thermal deformation activation energy and apparent stress index and to construct a thermal deformation constitutive model. Results reveal that the flow stress is strongly dependent on temperature and strain rate and it increases with temperature decreasing and strain rate increasing. The DRX phenomenon occurs more easily at comparably higher deformation temperatures and lower strain rates. Based on the method for solving the inflection point via cubic polynomial fitting of strain hardening rate(h) versus strain(e) curves, the ratio of critical strain(ec) to peak strain(ep) during DRX was precisely predicted. The nucleation mechanisms of DRX during thermal deformation mainly include the strain-induced grain boundary(GB)migration, grain fragmentation, and subgrain coalescence.
基金supported by the National Natural Science Foundation of China(NSFC,Grant Nos.11988101 and 11725313)supported by the Open Project Program of the Key Laboratory of FAST,Chinese Academy of Sciences+11 种基金support from the National Natural Science Foundation of China(NSFC,Grant No.U2031117)the Youth Innovation Promotion Association CAS(id.2021055)CAS Project for Young Scientists in Basic Research(grant YSBR-006)the Cultivation Project for FAST Scientific Payoff and Research Achievement of CAMS-CASsupported by the Key Research Project of Zhejiang Lab No.2021PE0AC0supported by the National Natural Science Foundation of China(NSFC,Grant Nos.11988101 and 11833009)the Key Research Program of the Chinese Academy of Sciences(Grant No.QYZDJ-SSWSLH021)supported by the Cultivation Project for the FAST scientific Payoff and Research Achievement of CAMSCASsupported by National SKA Program of China 2020SKA0120200National Natural Science Foundation of China(NSFC,Grant No.12041303)the CAS-MPG LEGACY projectfunding from the MaxPlanck Partner Group。
文摘We report the properties of more than 800 bursts detected from the repeating fast radio burst(FRB)source FRB20201124A with the Five-hundred-meter Aperture Spherical radio Telescope(FAST)during an extremely active episode on UTC 2021 September 25–28 in a series of four papers.In this second paper of the series,we study the energy distribution of 881 bursts(defined as significant signals separated by dips down to the noise level)detected in the first four days of our 19 hr observational campaign spanning 17 days.The event rate initially increased exponentially but the source activity stopped within 24 hr after the 4th day.The detection of 542 bursts in one hour during the fourth day marked the highest event rate detected from one single FRB source so far.The bursts have complex structures in the time-frequency space.We find a double-peak distribution of the waiting time,which can be modeled with two log-normal functions peaking at 51.22 ms and 10.05 s,respectively.Compared with the emission from a previous active episode of the source detected with FAST,the second distribution peak time is smaller,suggesting that this peak is defined by the activity level of the source.We calculate the isotropic energy of the bursts using both a partial bandwidth and a full bandwidth and find that the energy distribution is not significantly changed.We find that an exponentially connected broken-power law function can fit the cumulative burst energy distribution well,with the lower and higher-energy indices being-1.22±0.01 and-4.27±0.23,respectively.Assuming a radio radiative efficiency ofη_(r)=10^(-4),the total isotropic energy of the bursts released during the four days when the source was active is already 3.9×10^(46)erg,exceeding~23%of the available magnetar dipolar magnetic energy.This challenges the magnetar models which invoke an inefficient radio emission(e.g.,synchrotron maser models).
基金supported by the National SKA Program of China(Nos.2020SKA0120200 and 2020SKA0120100)the National Natural Science Foundation of China(Grant Nos.12041303,11873067 and 12041304)+4 种基金the National Key R&D Program of China(Nos.2017YFA0402600,2021YFA0718500 and 2017YFA0402602)the CAS-MPG LEGACY project,the Max-Planck Partner Group,the Key Research Project of Zhejiang Lab(No.2021PE0AC0)supported by the National Natural Science Foundation of China(NSFC,Nos.11988101 and 11833009)the Key Research Program of the Chinese Academy of Sciences(Grant No.QYZDJ-SSWSLH021)supported by the Cultivation Project for the FAST scientific Payoff and Research Achievement of CAMS-CAS。
文摘We report the properties of more than 800 bursts detected from the repeating fast radio burst(FRB)source FRB 20201124A with the Five-hundred-meter Aperture Spherical radio Telescope during an extremely active episode on UTC 2021 September 25th-28th in a series of four papers.In this fourth paper of the series,we present a systematic search of the spin period and linear acceleration of the source object from both 996 individual pulse peaks and the dedispersed time series.No credible spin period was found from this data set.We rule out the presence of significant periodicity in the range between 1 ms and 100 s with a pulse duty cycle<0.49±0.08(when the profile is defined by a von-Mises function,not a boxcar function)and linear acceleration up to 300 m s^(-2)in each of the four one-hour observing sessions,and up to 0.6 m s^(-2)in all 4 days.These searches contest theoretical scenarios involving a 1 ms–100 s isolated magnetar/pulsar with surface magnetic field<10^(15)G and a small duty cycle(such as in a polar-cap emission mode)or a pulsar with a companion star or black hole up to 100 M_(⊙)and P_(b)>10 hr.We also perform a periodicity search of the fine structures and identify 53 unrelated millisecond-timescale“periods”in multicomponents with the highest significance of 3.9σ.The“periods”recovered from the fine structures are neither consistent nor harmonically related.Thus they are not likely to come from a spin period.We caution against claiming spin periodicity with significance below~4σwith multi-components from one-off FRBs.We discuss the implications of our results and the possible connections between FRB multi-components and pulsar microstructures.
文摘The aim of this study is to evaluate the uncertainty of 2πα and 2πβ surface emission rates using the windowless multiwire proportional counter method.This study used the Monte Carlo method (MCM) to validate the conventional Guide to the Expression of Uncertainty in Measurement (GUM) method.A dead time measurement model for the two-source method was established based on the characteristics of a single-channel measurement system,and the voltage threshold correction factor measurement function was indirectly obtained by fitting the threshold correction curve.The uncertainty in the surface emission rate was calculated using the GUM method and the law of propagation of uncertainty.The MCM provided clear definitions for each input quantity and its uncertainty distribution,and the simulation training was realized with a complete and complex mathematical model.The results of the surface emission rate uncertainty evaluation for four radioactive plane sources using both methods showed the uncertainty’s consistency E_(n)<0.070 for the comparison of each source,and the uncertainty results of the GUM were all lower than those of the MCM.However,the MCM has a more objective evaluation process and can serve as a validation tool for GUM results.
基金financially supported by the National Natural Science Foundation of China(Nos.51701028 and 51421001)the Opening Project of Jiangsu Province Key Laboratory of High-end Structural Materials(No.HSM1901)+1 种基金the Open Research Fund from the State Key Laboratory of Rolling and Automation(No.2020RALKFKT016)the Venture&Innovation Support Program for Chongqing Overseas Returnees(No.CX2018056)。
文摘In the present work, the orientation characteristics of residual grains during hot deformation of an age-hardening Ni-Fe-Cr alloy(Alloy 925) at different conditions were systematically analyzed through high-resolution electron back-scatter diffraction. Based on the measurement of the kernel average misorientation, the density of geometrical necessary dislocations(GNDs) was further calculated. The orientation-dependent deformation mechanism of the residual grains was also discussed using Schmid factor difference ratio(SFDR) analysis. The results show that the deformed microstructure features typical "necklace" structures. Many distorted twin boundaries can be observed within the residual grains at 950 ℃. When the deformation temperature increases to 1150 ℃, the volume fraction of dynamic recrystallization(DRX) increases, leading to the extensive formation of primary Σ3 twin boundaries. Additionally, the GNDs are widely distributed in the residual grains, while they are rare for the recrystallized grains. The maximum GND density value can be obtained at the interface of "soft–hard" grains. The GND density also increases at higher strain rates, and the number of DRX grains significantly affects the distribution of the GND density. Moreover, based on the calculations and SFDR analysis, it can be summarized that the {100} and {111} grains are prone to deform in the uniserial slip mode and the multiple slip mode, respectively.
基金This work was supported by China Postdoctoral Science Foundation(No.2019M661738)Opening Project of Jiangsu Key Laboratory of Advanced Structural Materials and Application Technology(No.ASMA202002)Postgraduate Research and Practice Innovation Program of Jiangsu Province(No.SJCX21_1703).
文摘The hot deformation behavior of 316H stainless steel used in the 4th-generation nuclear systems was investigated by thermal compression tests at 1000–1150 C and 0.01–10 s^(-1).It was found that true stress firstly increased and then decreased with the increasing strain rate with a threshold of 1 s^(-1).Electron backscatter diffraction was used to analyze the microstructure evolution.Discontinuous dynamic recrystallization(DDRX)was the dominant dynamic recrystallization(DRX)mechanism,while continuous dynamic recrystallization(CDRX)was the supplementary one.DDRX happened before CDRX and provided additional nucleation sites for the latter.Twin grain boundaries(R3)appeared in DRX grains due to growth accidents.As the length fraction of R3 increased,the coincidence site lattice(CSL)boundary transition began to occur,forming R9 and R27.After the occurrence of full DRX,the growth and annexation of DRX grains were easy to be promoted,in which progress both equiaxed grains and CSL boundaries disappeared.The ideal deformation microstructure with fine and uniform DRX grains,which was accompanied by a high length fraction of CSL boundaries,appeared at 1000℃–0.01 s^(-1),1050℃–0.01–0.1 s^(-1),1100℃–0.1–1 s^(-1) and 1150℃–1–10 s^(-1).That is,the deformation conditions mentioned above were the preferable thermal forming parameters for 316H stainless steel in actual productions.
基金supported by the National Science and Technology Major Project(2017ZX10204401001002)the National Key Research and Development Project of China(2017ZX10204401001002008)+1 种基金the Key Research and Development Project of Zhejiang Province(2020C03123)Zhejiang Provincial Nature Science Foundation of China(LED20H19001)。
文摘Understanding the immunological characteristics of monocytes-including the characteristics associated with fibrosis-in severe coronavirus disease 2019(COVID-19)is crucial for understanding the pathogenic mechanism of the disease and preventing disease severity.In this study,we performed single-cell transcriptomic sequencing of peripheral blood samples collected from six healthy controls and 14 COVID-19samples including severe,moderate,and convalescent samples from three severely/critically ill and four moderately ill patients.We found that the monocytes were strongly remodeled in the severely/critically ill patients with COVID-19,with an increased proportion of monocytes and seriously reduced diversity.In addition,we discovered two novel severe-disease-specific monocyte subsets:Mono 0 and Mono 5.These subsets expressed amphiregulin(AREG),epiregulin(EREG),and cytokine interleukin-18(IL-18)gene,exhibited an enriched erythroblastic leukemia viral oncogene homolog(ErbB)signaling pathway,and appeared to exhibit pro-fibrogenic and pro-inflammation characteristics.We also found metabolic changes in Mono 0 and Mono 5,including increased glycolysis/gluconeogenesis and an increased hypoxia inducible factor-1(HIF-1)signaling pathway.Notably,one pre-severe sample displayed a monocyte atlas similar to that of the severe/critical samples.In conclusion,our study discovered two novel severedisease-specific monocyte subsets as potential predictors and therapeutic targets for severe COVID-19.Overall,this study provides potential predictors for severe disease and therapeutic targets for COVID-19 and thus provides a resource for further studies on COVID-19.
基金supported by the National Natural Science Foundation of China(Grant No.81974355 and No.82172524).
文摘Objective This study aimed to evaluate and compare the effectiveness of knowledge base-optimized and unoptimized large language models(LLMs)in the field of orthopedics to explore optimization strategies for the application of LLMs in specific fields.Methods This research constructed a specialized knowledge base using clinical guidelines from the American Academy of Orthopaedic Surgeons(AAOS)and authoritative orthopedic publications.A total of 30 orthopedic-related questions covering aspects such as anatomical knowledge,disease diagnosis,fracture classification,treatment options,and surgical techniques were input into both the knowledge base-optimized and unoptimized versions of the GPT-4,ChatGLM,and Spark LLM,with their generated responses recorded.The overall quality,accuracy,and comprehensiveness of these responses were evaluated by 3 experienced orthopedic surgeons.Results Compared with their unoptimized LLMs,the optimized version of GPT-4 showed improvements of 15.3%in overall quality,12.5%in accuracy,and 12.8%in comprehensiveness;ChatGLM showed improvements of 24.8%,16.1%,and 19.6%,respectively;and Spark LLM showed improvements of 6.5%,14.5%,and 24.7%,respectively.Conclusion The optimization of knowledge bases significantly enhances the quality,accuracy,and comprehensiveness of the responses provided by the 3 models in the orthopedic field.Therefore,knowledge base optimization is an effective method for improving the performance of LLMs in specific fields.
基金supported in part by the State Key Lab of Advanced Metals and Materials in University of Science and Technology Beijing(No.2022-Z21)China Postdoctoral Science Foundation(No.2019M661738),Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.SJCX22_1860)Natural Science Foundation of Jiangsu Province(No.BK20220548).
文摘The effect of temperature on the tensile properties and deformation mechanism of GH4169 alloy has been systematically studied over a wide range of room temperature(RT)to 1000℃.The results indicate that the stress–strain curve of the alloy shows serrations at 200–600℃,and the character of the serrations changes from type A to type B and then to type C at different temperatures.The ultimate tensile strength of the alloy decreases gradually from RT to 600℃.The yield strength decreases slowly from RT to 700℃ but decreases rapidly above 800℃.Transmission electron microscopy analysis relieves that the primary deformation mechanism of the alloy below 500℃ is Orowan bypass mechanism.At temperatures between 600 and 700℃,the coordinated deformation of twins and cross-slip of dislocations are activated.The transformation of\upgamma^{\prime\prime}phase toδphase above 650℃ will decrease the strength.The primary deformation mechanism above 800℃ transforms into the repeated shearing of\upgamma^{\prime\prime}by dislocations to form multiple stacking faults.Recrystallized grains were observed above 800℃,and continuous dynamic recrystallization and discontinuous dynamic recrystallization were observed.The stress concentration caused by Nb-rich carbides is the cause of intracrystalline crack nucleation.At 700℃,grain boundary crack sprouting is caused by the combined effect of slip band impact on grain boundaries and grain boundary dislocation plugging.The relationship between the serrated flow behavior and the deformation mechanism has been discussed based on the experimental results.
基金the following agencies:National Natural Science Foundation of China(31860071)New Agricultural Research and Reform Practice Project of the Ministry of Education(2020114)+8 种基金Grassland Talent Innovation Team of Inner Mongolia Autonomous Region-Castor Molecular Breeding Research Innovative Talent Team(2022)Inner Mongolia University for Nationalities 2022 Basic Research Business Funds for Universities Directly under the Autonomous Region(237)the Natural Science Foundation of Inner Mongolia Autonomous Region(2021 MS03008)In 2023,the Department of Science and Technology of Inner Mongolia Autonomous Region Approved the Construction Project of Inner Mongolia Autonomous Region Key Laboratory of Castor Breeding and Comprehensive UtilizationInner Mongolia Autonomous Region Castor Industry Collaborative Innovation Center Open Fund Project(MDK2021011,MDK2022014)the Natural Science Foundation of Inner Mongolia Autonomous Region(2021BS03036)Inner Mongolia University for Nationalities Doctoral Research Start-Up Fund(BS672)Castor Industry Technology Innovation Inner Mongolia Autonomous Region Engineering Research Center Open Project(MDK2021004)Inner Mongolia Autonomous Region Castor Industry Collaborative Innovation Center Open Project(MDK2022016).
文摘Castor(Ricinus communis L.)is one of the top 10 oil crops in the world,and inflorescence is a trait that directly affects its yield.Phospholipase C(PLCs)is involved in many plant activities and metabolic processes.To study the functions of PLC family genes in the regulation of the inflorescence development of the female line of Lm-type castor aLmAB2,we determined the expression levels of six PLC family genes of three types of inflorescences of aLmAB2(isofemale line,female line,bisexual line)at different developmental stages.The results showed that the 6 genes of the castor PLC family had relative expression levels at different developmental stages of the three types of inflorescences.The subcellular location of all six protein products was the cell membrane.The six genes were heterologously overexpressed in Arabidopsis thaliana to obtain the T3 generation-resistant Arabidopsis thaliana plants.The results showed that the overexpression of six genes significantly promoted the maturation of Arabidopsis thaliana,the growth of lateral moss,and the development of flowers and pods,but the development of basal leaves and stem leaves of Arabidopsis thaliana was significantly inhibited.According to homology analysis,it is speculated that PLC2,PLC2M,PLC2N,PLC4,PLC4X2,and PLC6 genes have the same regulatory function.
基金This study was supported by the National Natural Science Foundation of China(31860071)Research and Reform Practice Project in New Agricultural Sciences of the Ministry of Education in 2020(2020114)+7 种基金Natural Science Foundation of Inner Mongolia Autonomous Region(2021MS03008)Inner Mongolia Autonomous Region Grassland Talents Innovation Team—Castor Molecular Breeding Research Innovative Talent Team Rolling Support Project(2022)Higher Education Teaching Reform Research Project of National Ethnic Affairs Commission in 2021(21082)Fundamental Research Funds in Higher Education Institutions of Inner Mongolia in 2022(237)Autonomous Region Basic Scientific Reasearch Business Fee Projest of Inner Mongolia MinZu University in 2023(225,227,244)Inner Mongolia Autonomous Region Castor Industry Collaborative Innovation Center Construction Project(MDK2021011,MDK2022014)Open Fund Project in State Key Laboratory of Castor Breeding of China’s National Ethnic Affairs Commission(MDK2021008)Science and Technology Research Project of Jilin Provincial Department of Education(JJKH20220010KJ).
文摘Castor(Ricinus communis L.)is one of ten oil crops in the world and has complex inflorescence styles.Generally,castor has three inflorescence types:single female inflorescence(SiFF),standard female inflorescence(StFF)and bisexual inflorescence(BF).StFF is realized as a restorer line and as a maintainer line,which was applied to castor hybrid breeding.However,the developmental mechanism of the three inflorescences is not clear.Therefore,we used proteomic techniques to analyze different inflorescence styles.A total of 72 diferentially abundant protein species(DAPs)were detected.These DAPs are primarily involved in carbon and energy metabolism and carbon fixation in the photosynthetic organism pathway.The results showed that DAPs are involved in photosynthesis to control the distribution of imported carbohydrates and exported photoassimilates and thus affect the inflorescence development of castor.In addition,these DAPs are also involved in cysteine and methionine metabolism.Quantitative real-time PCR(qRT-PCR)results demonstrated that the proteomics data collected in this study were reliable.Our findings indicate that the carbon cycle and amino acid metabolism influence the inflorescence development of castor.
