Sodium with low cost and high abundance is considered as a substitute element of lithium for batteries and supercapacitors,which need the appropriate host materials to accommodate the relatively large Na^(+) ions.Comp...Sodium with low cost and high abundance is considered as a substitute element of lithium for batteries and supercapacitors,which need the appropriate host materials to accommodate the relatively large Na^(+) ions.Compared to Li^(+) storage,Na^(+) storage makes higher demands on the structural optimization of perovskite bismuth ferrite(BiFeO_(3)).We propose a novel strategy of defect engineering on BiFeO_(3) through Na and V codoping for high-efficiency Na^(+) storage,to reveal the roles of oxygen vacancies and V ions played in the enhanced electrochemical energy storage performances of Na-ion capacitors.The formation of the oxygen vacancies in the Na and V codoped BiFeO_(3)(denoted as NV-BFO),is promoted by Na doping and suppressed by V doping,which can be demonstrated by XPS and EPR spectra.By the first-principles calculations,the oxygen vacancies and V ions in NV-BFO are confirmed to substantially lower the Na^(+)migration energy barriers through the space and electric field effects,to effectively promote the Na^(+) transport in the crystals.Electrochemical kinetic analysis of the NV-BFO//NV-BFO capacitors indicates the dominant capacitive-controlled capacity,which depends on fast Na^(+) deintercalation-intercalation process in the NV-BFO electrode.The NV-BFO//NV-BFO capacitors open up a new avenue for developing highperformance Na-ion capacitors.展开更多
AIM： To observe the therapeutic effects of liposomeencapsulated adriamycin （LADM） on hepatoma in comparison with adriamycin solution （FADM） and adriarnycin plus blank liposome （ADM ＋ BL） administered into the ...AIM： To observe the therapeutic effects of liposomeencapsulated adriamycin （LADM） on hepatoma in comparison with adriamycin solution （FADM） and adriarnycin plus blank liposome （ADM ＋ BL） administered into the hepatic artery of rats. METHODS： LADM was prepared by pH gradient-driven method. Normal saline, FADM （2 mg/kg）, ADM＋BL （2 mg/kg）, and LADM （2 mg/kg） were injected via the hepatic artery in rats bearing liver W256 carcinosarcoma, which were divided into four groups randomly. The therapeutic effects were evaluated in terms of survival time, tumor enlargement ratio, and tumor necrosis degree. The difference was determined with ANOVA and Dunnett test and log rank test. RESULTS： Compared to FADM or ADM ＋ BL, LADM produced a more significant tumor inhibition （tumor volume ratio： 1.243±0.523 vs 1.883±0.708, 1.847±0.661, P 〈 0.01）, and more extensive tumor necrosis. The increased life span was prolonged significantly in rats receiving LADM compared with FADM or ADM＋BL （231.48 vs 74.66, 94.70） （P 〈 0.05）. CONCLUSION： The anticancer efficacies of adriamycin on hepatoma can be strongly improved by liposomal encapsulation through hepatic arterial administration.展开更多
AIM: To investigate whether Tg737 is regulated by micro RNA-548a-5p(mi R-548a-5p), and correlates with hepatocellular carcinoma(HCC) cell proliferation and apoptosis.METHODS: Assays of loss of function of Tg737 were p...AIM: To investigate whether Tg737 is regulated by micro RNA-548a-5p(mi R-548a-5p), and correlates with hepatocellular carcinoma(HCC) cell proliferation and apoptosis.METHODS: Assays of loss of function of Tg737 were performed by the colony formation assay, CCK assay and cell cycle assay in HCC cell lines. The interaction between mi R-548a-5p and its downstream target, Tg737, was evaluated by a dual-luciferase reporter assay and quantitative real-time polymerase chain reaction. Tg737 was then up-regulated in HCC cells to evaluate its effect on mi R-548a-5p regulation. Hep G2 cells stably overexpressing mi R-548a-5p or mi R-control were also subcutaneously inoculated into nude mice to evaluate the effect of mi R-548a-5p up-regulation on in vivo tumor growth. As the final step, the effect of mi R-548a-5p on the apoptosis induced by cisplatin was evaluated by flow cytometry.RESULTS: Down-regulation of Tg737, which is a target gene of mi R-548a-5p, accelerated HCC cell proliferation, and mi R-548a-5p promoted HCC cell proliferation in vitro and in vivo. Like the downregulation of Tg737, overexpression of mi R-548a-5p in HCC cell lines promoted cell proliferation, increased colony forming ability and hampered cell apoptosis. In addition, mi R-548a-5p overexpression increased HCC cell growth in vivo. Mi R-548a-5p downregulated Tg737 expression through direct contact with its 3' untranslated region(UTR), and mi R-548a-5p expression was negatively correlated with Tg737 levels in HCC specimens. Restoring Tg737(without the 3'UTR) significantly hampered mi R-548a-5p induced cell proliferation, and rescued the mi R-548a-5p induced cell proliferation inhibition and apoptosis induced by cisplatin.CONCLUSION: Mi R-548a-5p negatively regulates the tumor inhibitor gene Tg737 and promotes tumorigenesis in vitro and in vivo, indicating its potential as a novel therapeutic target for HCC.展开更多
Three-dimensional(3D) printing provides a promising way to fabricate biodegradable scaffolds with designer architectures for the regeneration of various tissues.However,the existing3D-printed scaffolds commonly suffer...Three-dimensional(3D) printing provides a promising way to fabricate biodegradable scaffolds with designer architectures for the regeneration of various tissues.However,the existing3D-printed scaffolds commonly suffer from weak cell-scaffold interactions and insufficient cell organizations due to the limited resolution of the 3D-printed features.Here,composite scaffolds with mechanically-robust frameworks and aligned nanofibrous architectures are presented and hybrid manufactured by combining techniques of 3D printing,electrospinning,and unidirectional freeze-casting.It was found that the composite scaffolds provided volume-stable environments and enabled directed cellular infiltration for tissue regeneration.In particular,the nanofibrous architectures with aligned micropores served as artificial extracellular matrix materials and improved the attachment,proliferation,and infiltration of cells.The proposed scaffolds can also support the adipogenic maturation of adipose-derived stem cells(ADSCs)in vitro.Moreover,the composite scaffolds were found to guide directed tissue infiltration and promote nearby neovascularization when implanted into a subcutaneous model of rats,and the addition of ADSCs further enhanced their adipogenic potential.The presented hybrid manufacturing strategy might provide a promising way to produce additional topological cues within 3D-printed scaffolds for better tissue regeneration.展开更多
BACKGROUND Myiasis is a rare but risky pathology caused by a parasitic infestation of humansand animals by the dipterous larva. Oral myiasis occurs when soft tissues of theoral cavity are invaded by the larvae of flie...BACKGROUND Myiasis is a rare but risky pathology caused by a parasitic infestation of humansand animals by the dipterous larva. Oral myiasis occurs when soft tissues of theoral cavity are invaded by the larvae of flies. It is not a common disease for thereason that the oral cavity is not easily reachable for the fly to lay eggs. But it cancause pain, infection, uncomfortable feeling when the worms move, tissuedestruction and/or even life-threatening hemorrhages.CASE SUMMARY We reported a case of oral myiasis after cerebral infarction in a 78-year-old malepatient from southern China (Guangdong Zhanjiang). As a result of cerebralinfarction, he suffered from right hemiplegia, mobility and mental decline forabout 3 mo. He had difficulty swallowing and was fed via a feeding tube. Hemostly engaged in mouth breathing and had poor oral and dental hygiene. Morethan 20 live larvae were collected from the patient’s oral cavity, which werelocalized in the maxillary gingiva, the mandibular gingiva and the tongue. Thepatient recovered after the routine oral cleaning, removal of maggots,debridement and anti-infection treatment.CONCLUSION Early diagnosis and treatment of this infestation are essential due to thebothersome symptoms, such as inflammation, intense anxiety over the larvaemovement, possible serious complications, etc. Clinical staff should be familiarwith this infestation, and this disease should be considered, especially inphysically and mentally disabled patients or those at significant risk for infection.Necessary measures, including good sanitation, personal and environmentalhygiene and special care should be adopted so as to prevent this disease.展开更多
BACKGROUND:Very good therapeutic effect has been obtained for limb negative pressure in treating limb ischemic diseases.But its mechanism in treating diabetic foot needs further investigation.OBJECTIVE:To observe the ...BACKGROUND:Very good therapeutic effect has been obtained for limb negative pressure in treating limb ischemic diseases.But its mechanism in treating diabetic foot needs further investigation.OBJECTIVE:To observe the changes of calcitonin gene related peptide(CGRP)in sensory nerve fiber of skin of dogs with diabetic foot before and after limb negative pressure treatment,and make a comparison with normal dogs,then analyze the mechanism of limb negative pressure in treating diabetic foot.DESIGN:A randomized controlled animal experiment.SETTING:Third Department of General Surgery,Xijing Hospital,Fourth Military Medical University of Chinese PLA.MATERIALS:Fifteen healthy hybrid dogs,of either gender,weighing 12-18 kg,were provided by Animal Room,Xijing Hospital of the Fourth Military Medical University of Chinese PLA.After being numbered,the involved dogs were randomly assigned into 3 groups:treatment group,model group and normal control group,5 dogs each.Limb negative pressure therapeutic machine(self-made);Anti-CGRP serum(primary antibody,Watpa Company,England);rabbit anti-BigG(second antibody),ABC compound solution,DAB reagent,glucose oxidase reagent,Trixon-X100 solution(Sigma company,USA),ammonium nickelous sulfate reagent(Xi'an Chemical Reagent Factory),neutral gum-solution(import,Shanghai Reagent Co.,Ltd.);Leitz1400 sliding freezing microtome(Leitz Company,Germany);Leica1800 cryostat microtome(Leica Company,Germany);Quantimet570 image analyzer(Leica Company,Germany).METHODS:This experiment was carried out in the Animal Room,Xijing Hospital,Fourth Military Medical University of Chinese PLA from October 2005 to June 2006.①After the dogs in the treatment and model groups were anesthetized,their pancreatic tissues were removed completely and animal models of typeⅠdiabetes mellitus were developed.All the femoral artery branches including deep femoral artery were ligated and cut off.A helical wire was inserted into the distal femoral artery cavity for narrowing femoral artery 1/2,then contributing to ischemic status of this limb,thus,dog models of diabetic foot of posterior limbs were developed.When the dogs in the treatment group were successfully modeled,negative pressure treatment was conducted on their affected limbs after 14 days.When the dogs were lightly anesthetized,depilation was performed on the base of left posterior limb in ring form.Then,their depilated limbs were placed in self-made negative pressure cabin for 15 minutes of-12 kPa negative pressure treatment every day within 10 days successively.Negative pressure treatment on affected limbs was omitted in the model group.The dogs in the normal control group were not modeled or given negative pressure treatment.②After 10 days of treatment,3 groups of animals all subjected to immunohistochemical staining of toe skin of affected limb for detecting CGRP-immunoreactive positive nerve fibers.Analysis of variance and LSD-t test were used for comparison of measurement data.MAIN OUTCOME MEASURES:Changes of CGRP in sensory nerve fibers of skin of 3 groups of animals.RESULTS:CGRP-immunoreactive positive nerve fibers in the skin of animals of model group were(53.24±5.6)μm/mm2,which were significantly more than those of control group[(11.73±2.3)μm/mm2,t=16.94,P<0.01].CGRP-immunoreactive positive nerve fibers in the skin of animals of treatment group were(32.49±2.9)μm/mm2,which were less than those of model group(t=8.47,P<0.01),but were still more than those of normal control group(t=8.47,P<0.01).CONCLUSION:①Following the onset of diabetic foot,CGRP-immunoreactive positive nerve fibers in the skin of distal limb are obviously increased,which may be a self-protective mechanism of body.②Limb negative pressure treatment can treat diabetic foot,which attributes to that the release of CGRP in the sensory nerve fibers can promote vasodilatation.展开更多
AIM： To investigate the inhibitory effect of VP22 fusion protein-based dominant negative （DN） mutant on Hepatitis Bvrus （HBV） replication. METHODS： Full-length or truncated fragment of VP22 was fused to C termin...AIM： To investigate the inhibitory effect of VP22 fusion protein-based dominant negative （DN） mutant on Hepatitis Bvrus （HBV） replication. METHODS： Full-length or truncated fragment of VP22 was fused to C terminal of HBV core protein （HBc）, and subcloned into pcDNA3.1 （-） vector, yielding eukaryotic expression plasmids of DN mutant. After transfection into HepG2.2.15 cells, the expression of DN mutant was identified by immunofluorescence staining. The inhibitory effect of DN mutant on HBV replication was indexed as the supernatant HBsAg concentration determined by RIA and HBV-DNA content by fluorescent quantification-PCR （FQ-PCR）. Meanwhile, metabolism of HepG2.2.25 cells was evaluated by MTI- colorimetry. RESULTS： VP22-based DN mutants and its truncated fragment were expressed in HepG2.2.15 cells, and had no toxic effect on host cells. DN mutants could inhibit HBV replication and the transduction ability of mutantbearing protein had a stronger inhibitory effect on HBV replication. DN mutants with full length of VP22 had the strongest inhibitory effect on HBV replication, reducing the HBsAg concentration by 81.94%, and the HBV-DNA content by 72.30%. MTT assay suggested that there were no significant differences in cell metabolic activity between the groups. CONCLUSION： VP22-based DN mutant can inhibit HBV replication effectively.展开更多
For a class of nonlinear systems with sensor uncertainty,the problem of adaptive output feedback stabilisation is investigated in this paper.It is assumed that only the output is available for feedback control,which s...For a class of nonlinear systems with sensor uncertainty,the problem of adaptive output feedback stabilisation is investigated in this paper.It is assumed that only the output is available for feedback control,which suffers from sensor uncertainty.At the same time,the nonlinear function in the system model has an unknown growth rate.To solve this problem,a set of state observers is designed to estimate the unknown system states based on the uncertain output,and an adaptive output-feedback control is designed based on this.By using the corollary of Barbalet's Lemma,it is shown that the equilibrium of the closed-loop system is asymptotically stable.Finally,simulation results verify the effectiveness of the control scheme.展开更多
Food allergy is a growing health issue worldwide and the demand for sensitive,robust and high-throughput analytical methods is rising.In recent years,mass spectrometry-based methods have been established for multiple ...Food allergy is a growing health issue worldwide and the demand for sensitive,robust and high-throughput analytical methods is rising.In recent years,mass spectrometry-based methods have been established for multiple food allergen detection.In the present study,a novel method was developed for the simultaneous detection of almond,cashew,peanut,and walnut allergens in bakery foods using liquid chromatography–mass spectrometry.Proteins unique to these four ingredients were extracted,followed by trypsin digestion,quadrupole time-of-flight(Q-TOF)mass spectrometry and bioinformatics analysis.The raw data were processed by de-novo sequencing module plus PEAKS DB(database search)module of the PEAKS software to screen peptides specific to each nut species.The thermal stability and uniqueness of these candidate peptides were further verified using triple quadrupole mass spectrometry(QQQ-MS)in multiple reaction monitoring(MRM)mode.Each nut species was represented by four peptides,all of which were validated for label-free quantification(LFQ).Calibration curves were constructed with good linearity and correlation coefficient(r 2)greater than 0.99.The limits of detection(LODs)were determined to range from 0.11 to 0.31 mg/kg,and were compared with the reference doses proposed by Voluntary Incidental Trace Allergen Labelling(VITAL).The recoveries of the developed method in incurred bakery food matrices ranged from 72.5%to 92.1%with relative standard deviations(RSD)of<5.2%.The detection of undeclared allergens in commercial bakery food samples confirmed the presence of these allergens.In conclusion,this method provides insight into the qualitative and quantitative detection of trace levels of nut allergens in bakery foods.展开更多
To the Editor:In the last decade,neoadjuvant chemotherapy(NAC)has become a well-accepted treatment option for breast cancer,although few detailed description of NAC in China has yet been reported.[1]A previous study f...To the Editor:In the last decade,neoadjuvant chemotherapy(NAC)has become a well-accepted treatment option for breast cancer,although few detailed description of NAC in China has yet been reported.[1]A previous study found that among patients with clinically node-negative(cN0)breast cancer,97.7%(432/442)with breast pathologic complete response(bpCR)had ypN0(absence of metastases in the axillary lymph nodes);and 71.6%(882/1232)without bpCR achieved ypN0(P<0.001).As for human epidermal growth factor receptor 2(HER2)positive or triple-negative breast cancer(TNBC)that achieved bpCR.展开更多
Neoadjuvant therapy can yield clinical benefits in earlystage breast cancer with indications.The indications,treatment regimens,and efficacy of neoadjuvant therapy have been investigated,which have contributed valuabl...Neoadjuvant therapy can yield clinical benefits in earlystage breast cancer with indications.The indications,treatment regimens,and efficacy of neoadjuvant therapy have been investigated,which have contributed valuable clinical evidence.[1,2]The Chinese Society of Breast Surgery convened a panel of breast disease experts to review and discuss the literature on neoadjuvant therapy for breast cancer and evaluate relevant evidence by referring to the Grading of Recommendations,Assessment,Development,and Evaluations(GRADE)system.Taking into account China’s specific circumstances,the society and participating experts developed the Clinical Practice Guidelines for Neoadjuvant Therapy for Early-stage Breast Cancer(Version 2022)to serve as a reference for breast surgeons in China.展开更多
Mechanoluminescent materials that convert mechanical stimuli to light emission have attracted extensive attention for potential applications in human-machine interactions. Here, we report a simple and available novel ...Mechanoluminescent materials that convert mechanical stimuli to light emission have attracted extensive attention for potential applications in human-machine interactions. Here, we report a simple and available novel approach for the oxygen-assisted preparation of ZnS：Mn particles by solid-state reaction at atmospheric pressure without the formation of the corresponding oxides. The existence of O2 has a positive impact on the formation of S vacancies in wurtzite-phase ZnS, leading to the introduction of Mn2＋ ion luminescent centers and shallow donor levels, which can improve the electron-hole recombination rate. The O2 ratio and Mn2＋ ion doping concentration have significant effects on the luminous efficienc）5 which is optimal at 1%-20% and 1 at.%-2 at.% respectively. In addition, a device based on the piezo-photonic effect with excellent pressure sensitivity of 0.032 MPa-1 was fabricated, which can map the two-dimensional pressure distribution ranging from 2.2 to 40.6 MPa in situ. This device can be applied to real-time pressure mapping, smart sensor networks, high-level security systems, human-machine interfaces, and artificial skins.展开更多
Breast cancer is the most common malignancy among women in China.Approximately 304,000 new cases of breast cancer were diagnosed during 2015 in China,which ranked first in cancer diagnosis and accounted for 17.1%of al...Breast cancer is the most common malignancy among women in China.Approximately 304,000 new cases of breast cancer were diagnosed during 2015 in China,which ranked first in cancer diagnosis and accounted for 17.1%of all new cancers among women.[1,2]Both the US Preventive Services Task Force(USPSTF)[3]and National Comprehensive Cancer Network(NCCN)clinical practice guidelines[4]have made recommendations regarding risk assessment,to identify women with increased risk for breast cancer,in whom risk-based cancer screening and/or risk-reducing medication use should be provided so as to lower the individual breast cancer risk.展开更多
基金financial supports from National Natural Science Foundation of China(22005174 and 52271133)。
文摘Sodium with low cost and high abundance is considered as a substitute element of lithium for batteries and supercapacitors,which need the appropriate host materials to accommodate the relatively large Na^(+) ions.Compared to Li^(+) storage,Na^(+) storage makes higher demands on the structural optimization of perovskite bismuth ferrite(BiFeO_(3)).We propose a novel strategy of defect engineering on BiFeO_(3) through Na and V codoping for high-efficiency Na^(+) storage,to reveal the roles of oxygen vacancies and V ions played in the enhanced electrochemical energy storage performances of Na-ion capacitors.The formation of the oxygen vacancies in the Na and V codoped BiFeO_(3)(denoted as NV-BFO),is promoted by Na doping and suppressed by V doping,which can be demonstrated by XPS and EPR spectra.By the first-principles calculations,the oxygen vacancies and V ions in NV-BFO are confirmed to substantially lower the Na^(+)migration energy barriers through the space and electric field effects,to effectively promote the Na^(+) transport in the crystals.Electrochemical kinetic analysis of the NV-BFO//NV-BFO capacitors indicates the dominant capacitive-controlled capacity,which depends on fast Na^(+) deintercalation-intercalation process in the NV-BFO electrode.The NV-BFO//NV-BFO capacitors open up a new avenue for developing highperformance Na-ion capacitors.
文摘AIM： To observe the therapeutic effects of liposomeencapsulated adriamycin （LADM） on hepatoma in comparison with adriamycin solution （FADM） and adriarnycin plus blank liposome （ADM ＋ BL） administered into the hepatic artery of rats. METHODS： LADM was prepared by pH gradient-driven method. Normal saline, FADM （2 mg/kg）, ADM＋BL （2 mg/kg）, and LADM （2 mg/kg） were injected via the hepatic artery in rats bearing liver W256 carcinosarcoma, which were divided into four groups randomly. The therapeutic effects were evaluated in terms of survival time, tumor enlargement ratio, and tumor necrosis degree. The difference was determined with ANOVA and Dunnett test and log rank test. RESULTS： Compared to FADM or ADM ＋ BL, LADM produced a more significant tumor inhibition （tumor volume ratio： 1.243±0.523 vs 1.883±0.708, 1.847±0.661, P 〈 0.01）, and more extensive tumor necrosis. The increased life span was prolonged significantly in rats receiving LADM compared with FADM or ADM＋BL （231.48 vs 74.66, 94.70） （P 〈 0.05）. CONCLUSION： The anticancer efficacies of adriamycin on hepatoma can be strongly improved by liposomal encapsulation through hepatic arterial administration.
基金Supported by National Natural Science Foundation of ChinaNo.81272648
文摘AIM: To investigate whether Tg737 is regulated by micro RNA-548a-5p(mi R-548a-5p), and correlates with hepatocellular carcinoma(HCC) cell proliferation and apoptosis.METHODS: Assays of loss of function of Tg737 were performed by the colony formation assay, CCK assay and cell cycle assay in HCC cell lines. The interaction between mi R-548a-5p and its downstream target, Tg737, was evaluated by a dual-luciferase reporter assay and quantitative real-time polymerase chain reaction. Tg737 was then up-regulated in HCC cells to evaluate its effect on mi R-548a-5p regulation. Hep G2 cells stably overexpressing mi R-548a-5p or mi R-control were also subcutaneously inoculated into nude mice to evaluate the effect of mi R-548a-5p up-regulation on in vivo tumor growth. As the final step, the effect of mi R-548a-5p on the apoptosis induced by cisplatin was evaluated by flow cytometry.RESULTS: Down-regulation of Tg737, which is a target gene of mi R-548a-5p, accelerated HCC cell proliferation, and mi R-548a-5p promoted HCC cell proliferation in vitro and in vivo. Like the downregulation of Tg737, overexpression of mi R-548a-5p in HCC cell lines promoted cell proliferation, increased colony forming ability and hampered cell apoptosis. In addition, mi R-548a-5p overexpression increased HCC cell growth in vivo. Mi R-548a-5p downregulated Tg737 expression through direct contact with its 3' untranslated region(UTR), and mi R-548a-5p expression was negatively correlated with Tg737 levels in HCC specimens. Restoring Tg737(without the 3'UTR) significantly hampered mi R-548a-5p induced cell proliferation, and rescued the mi R-548a-5p induced cell proliferation inhibition and apoptosis induced by cisplatin.CONCLUSION: Mi R-548a-5p negatively regulates the tumor inhibitor gene Tg737 and promotes tumorigenesis in vitro and in vivo, indicating its potential as a novel therapeutic target for HCC.
基金financially supported by the National Key Research and Development Program of China(2018YFA0703003)the National Natural Science Foundation of China (52125501)+2 种基金the Key Research Project of Shaanxi Province (2021LLRH-08,2021GXLH-Z-028)the Program for Innovation Team of Shaanxi Province (2023-CX-TD-17)the Fundamental Research Funds for the Central Universities。
文摘Three-dimensional(3D) printing provides a promising way to fabricate biodegradable scaffolds with designer architectures for the regeneration of various tissues.However,the existing3D-printed scaffolds commonly suffer from weak cell-scaffold interactions and insufficient cell organizations due to the limited resolution of the 3D-printed features.Here,composite scaffolds with mechanically-robust frameworks and aligned nanofibrous architectures are presented and hybrid manufactured by combining techniques of 3D printing,electrospinning,and unidirectional freeze-casting.It was found that the composite scaffolds provided volume-stable environments and enabled directed cellular infiltration for tissue regeneration.In particular,the nanofibrous architectures with aligned micropores served as artificial extracellular matrix materials and improved the attachment,proliferation,and infiltration of cells.The proposed scaffolds can also support the adipogenic maturation of adipose-derived stem cells(ADSCs)in vitro.Moreover,the composite scaffolds were found to guide directed tissue infiltration and promote nearby neovascularization when implanted into a subcutaneous model of rats,and the addition of ADSCs further enhanced their adipogenic potential.The presented hybrid manufacturing strategy might provide a promising way to produce additional topological cues within 3D-printed scaffolds for better tissue regeneration.
文摘BACKGROUND Myiasis is a rare but risky pathology caused by a parasitic infestation of humansand animals by the dipterous larva. Oral myiasis occurs when soft tissues of theoral cavity are invaded by the larvae of flies. It is not a common disease for thereason that the oral cavity is not easily reachable for the fly to lay eggs. But it cancause pain, infection, uncomfortable feeling when the worms move, tissuedestruction and/or even life-threatening hemorrhages.CASE SUMMARY We reported a case of oral myiasis after cerebral infarction in a 78-year-old malepatient from southern China (Guangdong Zhanjiang). As a result of cerebralinfarction, he suffered from right hemiplegia, mobility and mental decline forabout 3 mo. He had difficulty swallowing and was fed via a feeding tube. Hemostly engaged in mouth breathing and had poor oral and dental hygiene. Morethan 20 live larvae were collected from the patient’s oral cavity, which werelocalized in the maxillary gingiva, the mandibular gingiva and the tongue. Thepatient recovered after the routine oral cleaning, removal of maggots,debridement and anti-infection treatment.CONCLUSION Early diagnosis and treatment of this infestation are essential due to thebothersome symptoms, such as inflammation, intense anxiety over the larvaemovement, possible serious complications, etc. Clinical staff should be familiarwith this infestation, and this disease should be considered, especially inphysically and mentally disabled patients or those at significant risk for infection.Necessary measures, including good sanitation, personal and environmentalhygiene and special care should be adopted so as to prevent this disease.
文摘BACKGROUND:Very good therapeutic effect has been obtained for limb negative pressure in treating limb ischemic diseases.But its mechanism in treating diabetic foot needs further investigation.OBJECTIVE:To observe the changes of calcitonin gene related peptide(CGRP)in sensory nerve fiber of skin of dogs with diabetic foot before and after limb negative pressure treatment,and make a comparison with normal dogs,then analyze the mechanism of limb negative pressure in treating diabetic foot.DESIGN:A randomized controlled animal experiment.SETTING:Third Department of General Surgery,Xijing Hospital,Fourth Military Medical University of Chinese PLA.MATERIALS:Fifteen healthy hybrid dogs,of either gender,weighing 12-18 kg,were provided by Animal Room,Xijing Hospital of the Fourth Military Medical University of Chinese PLA.After being numbered,the involved dogs were randomly assigned into 3 groups:treatment group,model group and normal control group,5 dogs each.Limb negative pressure therapeutic machine(self-made);Anti-CGRP serum(primary antibody,Watpa Company,England);rabbit anti-BigG(second antibody),ABC compound solution,DAB reagent,glucose oxidase reagent,Trixon-X100 solution(Sigma company,USA),ammonium nickelous sulfate reagent(Xi'an Chemical Reagent Factory),neutral gum-solution(import,Shanghai Reagent Co.,Ltd.);Leitz1400 sliding freezing microtome(Leitz Company,Germany);Leica1800 cryostat microtome(Leica Company,Germany);Quantimet570 image analyzer(Leica Company,Germany).METHODS:This experiment was carried out in the Animal Room,Xijing Hospital,Fourth Military Medical University of Chinese PLA from October 2005 to June 2006.①After the dogs in the treatment and model groups were anesthetized,their pancreatic tissues were removed completely and animal models of typeⅠdiabetes mellitus were developed.All the femoral artery branches including deep femoral artery were ligated and cut off.A helical wire was inserted into the distal femoral artery cavity for narrowing femoral artery 1/2,then contributing to ischemic status of this limb,thus,dog models of diabetic foot of posterior limbs were developed.When the dogs in the treatment group were successfully modeled,negative pressure treatment was conducted on their affected limbs after 14 days.When the dogs were lightly anesthetized,depilation was performed on the base of left posterior limb in ring form.Then,their depilated limbs were placed in self-made negative pressure cabin for 15 minutes of-12 kPa negative pressure treatment every day within 10 days successively.Negative pressure treatment on affected limbs was omitted in the model group.The dogs in the normal control group were not modeled or given negative pressure treatment.②After 10 days of treatment,3 groups of animals all subjected to immunohistochemical staining of toe skin of affected limb for detecting CGRP-immunoreactive positive nerve fibers.Analysis of variance and LSD-t test were used for comparison of measurement data.MAIN OUTCOME MEASURES:Changes of CGRP in sensory nerve fibers of skin of 3 groups of animals.RESULTS:CGRP-immunoreactive positive nerve fibers in the skin of animals of model group were(53.24±5.6)μm/mm2,which were significantly more than those of control group[(11.73±2.3)μm/mm2,t=16.94,P<0.01].CGRP-immunoreactive positive nerve fibers in the skin of animals of treatment group were(32.49±2.9)μm/mm2,which were less than those of model group(t=8.47,P<0.01),but were still more than those of normal control group(t=8.47,P<0.01).CONCLUSION:①Following the onset of diabetic foot,CGRP-immunoreactive positive nerve fibers in the skin of distal limb are obviously increased,which may be a self-protective mechanism of body.②Limb negative pressure treatment can treat diabetic foot,which attributes to that the release of CGRP in the sensory nerve fibers can promote vasodilatation.
基金Supported by the National Natural Science Foundation of China,No. 30400380
文摘AIM： To investigate the inhibitory effect of VP22 fusion protein-based dominant negative （DN） mutant on Hepatitis Bvrus （HBV） replication. METHODS： Full-length or truncated fragment of VP22 was fused to C terminal of HBV core protein （HBc）, and subcloned into pcDNA3.1 （-） vector, yielding eukaryotic expression plasmids of DN mutant. After transfection into HepG2.2.15 cells, the expression of DN mutant was identified by immunofluorescence staining. The inhibitory effect of DN mutant on HBV replication was indexed as the supernatant HBsAg concentration determined by RIA and HBV-DNA content by fluorescent quantification-PCR （FQ-PCR）. Meanwhile, metabolism of HepG2.2.25 cells was evaluated by MTI- colorimetry. RESULTS： VP22-based DN mutants and its truncated fragment were expressed in HepG2.2.15 cells, and had no toxic effect on host cells. DN mutants could inhibit HBV replication and the transduction ability of mutantbearing protein had a stronger inhibitory effect on HBV replication. DN mutants with full length of VP22 had the strongest inhibitory effect on HBV replication, reducing the HBsAg concentration by 81.94%, and the HBV-DNA content by 72.30%. MTT assay suggested that there were no significant differences in cell metabolic activity between the groups. CONCLUSION： VP22-based DN mutant can inhibit HBV replication effectively.
基金supported in part by the National Natural Science Foundation of China under Grant 62173052U22A20245+1 种基金the Research Projects of Chongqing Science and Technology Program(General Projects)under Grant cstc2021jcyj-msxmX1041in part by the Fund of National Engineering Research Center of Road Tunne under Grant No.NERCRT202202.
文摘For a class of nonlinear systems with sensor uncertainty,the problem of adaptive output feedback stabilisation is investigated in this paper.It is assumed that only the output is available for feedback control,which suffers from sensor uncertainty.At the same time,the nonlinear function in the system model has an unknown growth rate.To solve this problem,a set of state observers is designed to estimate the unknown system states based on the uncertain output,and an adaptive output-feedback control is designed based on this.By using the corollary of Barbalet's Lemma,it is shown that the equilibrium of the closed-loop system is asymptotically stable.Finally,simulation results verify the effectiveness of the control scheme.
基金the Key Science and Technology Program of the Market Supervision Administration of Jiangsu Province(No.KJ196035)the Key Science and Technology Program of the Market Supervision Administration of Nanjing(No.KJ2019043),China.
文摘Food allergy is a growing health issue worldwide and the demand for sensitive,robust and high-throughput analytical methods is rising.In recent years,mass spectrometry-based methods have been established for multiple food allergen detection.In the present study,a novel method was developed for the simultaneous detection of almond,cashew,peanut,and walnut allergens in bakery foods using liquid chromatography–mass spectrometry.Proteins unique to these four ingredients were extracted,followed by trypsin digestion,quadrupole time-of-flight(Q-TOF)mass spectrometry and bioinformatics analysis.The raw data were processed by de-novo sequencing module plus PEAKS DB(database search)module of the PEAKS software to screen peptides specific to each nut species.The thermal stability and uniqueness of these candidate peptides were further verified using triple quadrupole mass spectrometry(QQQ-MS)in multiple reaction monitoring(MRM)mode.Each nut species was represented by four peptides,all of which were validated for label-free quantification(LFQ).Calibration curves were constructed with good linearity and correlation coefficient(r 2)greater than 0.99.The limits of detection(LODs)were determined to range from 0.11 to 0.31 mg/kg,and were compared with the reference doses proposed by Voluntary Incidental Trace Allergen Labelling(VITAL).The recoveries of the developed method in incurred bakery food matrices ranged from 72.5%to 92.1%with relative standard deviations(RSD)of<5.2%.The detection of undeclared allergens in commercial bakery food samples confirmed the presence of these allergens.In conclusion,this method provides insight into the qualitative and quantitative detection of trace levels of nut allergens in bakery foods.
基金Beijing Medical Reward Foundation(No.YXJL-2016-0040-0012)
文摘To the Editor:In the last decade,neoadjuvant chemotherapy(NAC)has become a well-accepted treatment option for breast cancer,although few detailed description of NAC in China has yet been reported.[1]A previous study found that among patients with clinically node-negative(cN0)breast cancer,97.7%(432/442)with breast pathologic complete response(bpCR)had ypN0(absence of metastases in the axillary lymph nodes);and 71.6%(882/1232)without bpCR achieved ypN0(P<0.001).As for human epidermal growth factor receptor 2(HER2)positive or triple-negative breast cancer(TNBC)that achieved bpCR.
文摘Neoadjuvant therapy can yield clinical benefits in earlystage breast cancer with indications.The indications,treatment regimens,and efficacy of neoadjuvant therapy have been investigated,which have contributed valuable clinical evidence.[1,2]The Chinese Society of Breast Surgery convened a panel of breast disease experts to review and discuss the literature on neoadjuvant therapy for breast cancer and evaluate relevant evidence by referring to the Grading of Recommendations,Assessment,Development,and Evaluations(GRADE)system.Taking into account China’s specific circumstances,the society and participating experts developed the Clinical Practice Guidelines for Neoadjuvant Therapy for Early-stage Breast Cancer(Version 2022)to serve as a reference for breast surgeons in China.
基金The authors are thankful for support from National Natural Science Foundation of China （Nos. 51622205, 61675027, 61405040, 51432005, 61505010, and 51502018）, National Key R ＆ D project from Minister of Science and Technology, China （No. 2016YFA0202703）, National Postdoctoral Program for Innovative Talents （No. BX201600040）, China Postdoctoral Science Foundation Funded Project （No. 2016M600976） and the ＂Thousand Talents＂ program of China for pioneering researchers and innovative teams.
文摘Mechanoluminescent materials that convert mechanical stimuli to light emission have attracted extensive attention for potential applications in human-machine interactions. Here, we report a simple and available novel approach for the oxygen-assisted preparation of ZnS：Mn particles by solid-state reaction at atmospheric pressure without the formation of the corresponding oxides. The existence of O2 has a positive impact on the formation of S vacancies in wurtzite-phase ZnS, leading to the introduction of Mn2＋ ion luminescent centers and shallow donor levels, which can improve the electron-hole recombination rate. The O2 ratio and Mn2＋ ion doping concentration have significant effects on the luminous efficienc）5 which is optimal at 1%-20% and 1 at.%-2 at.% respectively. In addition, a device based on the piezo-photonic effect with excellent pressure sensitivity of 0.032 MPa-1 was fabricated, which can map the two-dimensional pressure distribution ranging from 2.2 to 40.6 MPa in situ. This device can be applied to real-time pressure mapping, smart sensor networks, high-level security systems, human-machine interfaces, and artificial skins.
文摘Breast cancer is the most common malignancy among women in China.Approximately 304,000 new cases of breast cancer were diagnosed during 2015 in China,which ranked first in cancer diagnosis and accounted for 17.1%of all new cancers among women.[1,2]Both the US Preventive Services Task Force(USPSTF)[3]and National Comprehensive Cancer Network(NCCN)clinical practice guidelines[4]have made recommendations regarding risk assessment,to identify women with increased risk for breast cancer,in whom risk-based cancer screening and/or risk-reducing medication use should be provided so as to lower the individual breast cancer risk.
