Objective:To observe the effects of perfusion of the gastrodin in abdominal aorta for alleviating the spinal cord ischemia reperfusion injury(SCIRI).Methods:A total of 36 New Zealand white rabbits were divided randoml...Objective:To observe the effects of perfusion of the gastrodin in abdominal aorta for alleviating the spinal cord ischemia reperfusion injury(SCIRI).Methods:A total of 36 New Zealand white rabbits were divided randomly into sham-operated group(group S),control group(group C) and gastrodin group(group G),12 rabbits for each group.Aorta abdominalis infrarenalis blocking method was applied to establish the SCIRI model.The changes of motor evoked potentials(MEPs) before the ischemia and on 30 min,60 min,6 h,12 h and 24 h of reperfusion of the gastrodin were respectively recorded,and the neurologic function score before the ischemia,on the 6 h,12 h and 24 h of the reperfusion of the gastrodin were assessed.And the changes of the concentration of serum neuron specific enolase(NSE),interleukin(IL)-l β and IL-8 were measured before the ischemia,after 45 min of ischemia,and on 30 min,60 min,6 h,12 h and 24 h of reperfusion of gastrodin.Then the levels of spinal cord nerve cells mitochondrial superoxide dismutase(SOD),reactive oxygen species(ROS),glutathione peroxidase(GSH-PX),malondialdehyde(MDA),total antioxidant capacity(T-AOC) and mitochondrial swelling degree(MSD) were tested and the histopathologic changes in spinal cord tissues were observed.Results:The levels of the NSE,IL-lfter the ischemβ,IL-8,ROS,MDA and MSD of group C were all significantly elevated aia(P<0.01);the levels of the spinal nerve cell mitochondria SOD,GSH-PX and T-AOC were all significantly reduced(P<0.01),MEPs and spinal cord tissue pathology were damaged significantly(P<0.01).The rate of motor neuron abnormalities and the damages of spinal cord tissue pathology of group G were significantly milder than those of group C(P<0.01);the levels of NSE,IL-lROS,MDA and MSD were significantly lower than those of group C(P< 0.01),but tβ,IL-8,he levels of SOD,GSH-PX and T-AOC were all significantly higher than those of group C(P<0.01),and the recovery of neurologic function score during the reperfusion of gastrodin was significantly faster than group C(P<0.01).Conclusions:Perfusion of the gastrodin in abdominal aorta can alleviate the spinal cord ischemiare perfusion injury by promoting the mitochondrial antioxidant capacity and inhibiting the inflammatory reaction.展开更多
Objective:The objective is to explore the potential mechanisms mediating the analgesic effect of electroacupuncture(EA)for neuropathic pain.Materials and Methods:Sprague-Dawley(SD)rats were used to establish a spared ...Objective:The objective is to explore the potential mechanisms mediating the analgesic effect of electroacupuncture(EA)for neuropathic pain.Materials and Methods:Sprague-Dawley(SD)rats were used to establish a spared nerve injury(SNI)model of neuropathic pain.The intensity of neuropathic pain was measured by assessing the mechanical withdrawal threshold and thermal withdrawal latency.Immunofluorescent analysis and western blotting were performed to evaluate the activation of microglia and Janus kinase 2/signal transducer and activator of transcription 3(JAK2/STAT3)signaling in the L4-6 spinal cord of SD rats.The infiltration of Th2 and expression of interleukin-10(IL-10)in the L4-6 spine were analyzed by flow cytometry and enzyme-linked immunosorbent assay.Results:Behavioral test results demonstrated that SNI-induced hyperalgesia was significantly ameliorated by EA stimulation at"Zusanli"(ST36)and"Yanglingquan"(GB34)points.In addition,EA therapy significantly suppressed microglia activation and phosphorylation of JAK2/STAT3 in the L4-6 spine of rats with SNI.Moreover,EA promoted the infiltration of Th2 and secretion of IL-10 in the L4-6 spine of rats with SNI.Conclusions:The study findings indicate that EA stimulation mediated the infiltration of Th2 in the spinal cord of rats with SNI.In addition,EA inhibited microglial activation in the L4-6 spine of rats with SNI.展开更多
基金supported by National Natural Science Foundation of China (Grant number:30672025)Science and Technology Department of Guizhou Province Foundation Project (Grant number:Qinkehe SY[2013]3063,Qinheke J[2013]2179,Qinkehe LH[2014]7021)
文摘Objective:To observe the effects of perfusion of the gastrodin in abdominal aorta for alleviating the spinal cord ischemia reperfusion injury(SCIRI).Methods:A total of 36 New Zealand white rabbits were divided randomly into sham-operated group(group S),control group(group C) and gastrodin group(group G),12 rabbits for each group.Aorta abdominalis infrarenalis blocking method was applied to establish the SCIRI model.The changes of motor evoked potentials(MEPs) before the ischemia and on 30 min,60 min,6 h,12 h and 24 h of reperfusion of the gastrodin were respectively recorded,and the neurologic function score before the ischemia,on the 6 h,12 h and 24 h of the reperfusion of the gastrodin were assessed.And the changes of the concentration of serum neuron specific enolase(NSE),interleukin(IL)-l β and IL-8 were measured before the ischemia,after 45 min of ischemia,and on 30 min,60 min,6 h,12 h and 24 h of reperfusion of gastrodin.Then the levels of spinal cord nerve cells mitochondrial superoxide dismutase(SOD),reactive oxygen species(ROS),glutathione peroxidase(GSH-PX),malondialdehyde(MDA),total antioxidant capacity(T-AOC) and mitochondrial swelling degree(MSD) were tested and the histopathologic changes in spinal cord tissues were observed.Results:The levels of the NSE,IL-lfter the ischemβ,IL-8,ROS,MDA and MSD of group C were all significantly elevated aia(P<0.01);the levels of the spinal nerve cell mitochondria SOD,GSH-PX and T-AOC were all significantly reduced(P<0.01),MEPs and spinal cord tissue pathology were damaged significantly(P<0.01).The rate of motor neuron abnormalities and the damages of spinal cord tissue pathology of group G were significantly milder than those of group C(P<0.01);the levels of NSE,IL-lROS,MDA and MSD were significantly lower than those of group C(P< 0.01),but tβ,IL-8,he levels of SOD,GSH-PX and T-AOC were all significantly higher than those of group C(P<0.01),and the recovery of neurologic function score during the reperfusion of gastrodin was significantly faster than group C(P<0.01).Conclusions:Perfusion of the gastrodin in abdominal aorta can alleviate the spinal cord ischemiare perfusion injury by promoting the mitochondrial antioxidant capacity and inhibiting the inflammatory reaction.
文摘Objective:The objective is to explore the potential mechanisms mediating the analgesic effect of electroacupuncture(EA)for neuropathic pain.Materials and Methods:Sprague-Dawley(SD)rats were used to establish a spared nerve injury(SNI)model of neuropathic pain.The intensity of neuropathic pain was measured by assessing the mechanical withdrawal threshold and thermal withdrawal latency.Immunofluorescent analysis and western blotting were performed to evaluate the activation of microglia and Janus kinase 2/signal transducer and activator of transcription 3(JAK2/STAT3)signaling in the L4-6 spinal cord of SD rats.The infiltration of Th2 and expression of interleukin-10(IL-10)in the L4-6 spine were analyzed by flow cytometry and enzyme-linked immunosorbent assay.Results:Behavioral test results demonstrated that SNI-induced hyperalgesia was significantly ameliorated by EA stimulation at"Zusanli"(ST36)and"Yanglingquan"(GB34)points.In addition,EA therapy significantly suppressed microglia activation and phosphorylation of JAK2/STAT3 in the L4-6 spine of rats with SNI.Moreover,EA promoted the infiltration of Th2 and secretion of IL-10 in the L4-6 spine of rats with SNI.Conclusions:The study findings indicate that EA stimulation mediated the infiltration of Th2 in the spinal cord of rats with SNI.In addition,EA inhibited microglial activation in the L4-6 spine of rats with SNI.
