Purpose-The indoor vibration compaction test(IVCT)was a key step in controlling the compaction quality for high-speed railway graded aggregate(HRGA),which currently had a research gap on the assessment indicators and ...Purpose-The indoor vibration compaction test(IVCT)was a key step in controlling the compaction quality for high-speed railway graded aggregate(HRGA),which currently had a research gap on the assessment indicators and compaction parameters.Design/methodology/approach-To address these issues,a novel multi-indicator IVCT method was proposed,including physical indicator dry density(ρd)and mechanical indicators dynamic stiffness(Krb)and bearing capacity coefficient(K20).Then,a series of IVCTs on HRGA under different compaction parameters were conducted with an improved vibration compactor,which could monitor the physical-mechanical indicators in real-time.Finally,the optimal vibration compaction parameters,including the moisture content(ω),the diameter-to-maximum particle size ratio(Rd),the thickness-to-maximum particle size ratio(Rh),the vibration frequency(f),the vibration mass(Mc)and the eccentric distance(re),were determined based on the evolution characteristics for the physical-mechanical indicators during compaction.Findings-All results indicated that theρd gradually increased and then stabilized,and the Krb initially increased and then decreased.Moreover,the inflection time of the Krb was present as the optimal compaction time(Tlp)during compaction.Additionally,optimal compaction was achieved whenωwas the water-holding content after mud pumping,Rd was 3.4,Rh was 3.5,f was the resonance frequency,and the ratio between the excitation force and the Mc was 1.8.Originality/value-The findings of this paper were significant for the quality control of HRGA compaction.展开更多
Deactivation of the mitochondrial pyruvate dehydrogenase complex(PDC)is important for the metabolic switching of cancer cell from oxidative phosphorylation to aerobic glycolysis.Studies examining PDC activity regulati...Deactivation of the mitochondrial pyruvate dehydrogenase complex(PDC)is important for the metabolic switching of cancer cell from oxidative phosphorylation to aerobic glycolysis.Studies examining PDC activity regulation have mainly focused on the phosphorylation of pyruvate dehydrogenase(E1),leaving other post-translational modifications largely unexplored.Here,we demonstrate that the acetylation of Lys 488 of pyruvate dehydrogenase complex component X(PDHX)commonly occurs in hepatocellular carcinoma,disrupting PDC assembly and contributing to lactate-driven epigenetic control of gene expression.PDHX,an E3-binding protein in the PDC,is acetylated by the p300 at Lys 488,impeding the interaction between PDHX and dihydrolipoyl transacetylase(E2),thereby disrupting PDC assembly to inhibit its activation.PDC disruption results in the conversion of most glucose to lactate,contributing to the aerobic glycolysis and H3K56 lactylation-mediated gene expression,facilitating tumor progression.These findings highlight a previously unrecognized role of PDHX acetylation in regulating PDC assembly and activity,linking PDHX Lys 488 acetylation and histone lactylation during hepatocellular carcinoma progression and providing a potential biomarker and therapeutic target for further development.展开更多
基金funded by the National Key R&D Program“Transportation Infrastructure”project(No.2022YFB2603400)the Technology Research and Development Plan Program of China State Railway Group Co.,Ltd.(No.Q2024T001)the National project pre research project of Suzhou City University(No.2023SGY019).
文摘Purpose-The indoor vibration compaction test(IVCT)was a key step in controlling the compaction quality for high-speed railway graded aggregate(HRGA),which currently had a research gap on the assessment indicators and compaction parameters.Design/methodology/approach-To address these issues,a novel multi-indicator IVCT method was proposed,including physical indicator dry density(ρd)and mechanical indicators dynamic stiffness(Krb)and bearing capacity coefficient(K20).Then,a series of IVCTs on HRGA under different compaction parameters were conducted with an improved vibration compactor,which could monitor the physical-mechanical indicators in real-time.Finally,the optimal vibration compaction parameters,including the moisture content(ω),the diameter-to-maximum particle size ratio(Rd),the thickness-to-maximum particle size ratio(Rh),the vibration frequency(f),the vibration mass(Mc)and the eccentric distance(re),were determined based on the evolution characteristics for the physical-mechanical indicators during compaction.Findings-All results indicated that theρd gradually increased and then stabilized,and the Krb initially increased and then decreased.Moreover,the inflection time of the Krb was present as the optimal compaction time(Tlp)during compaction.Additionally,optimal compaction was achieved whenωwas the water-holding content after mud pumping,Rd was 3.4,Rh was 3.5,f was the resonance frequency,and the ratio between the excitation force and the Mc was 1.8.Originality/value-The findings of this paper were significant for the quality control of HRGA compaction.
基金supported in part by National Natural Science Foundation of China(Grant Nos.81930083,82192893,81821001,and 82303217)National Key R&D Program of China(2022YFA1304504)+2 种基金the Chinese Academy of Sciences(XDB39000000)the Global Select Project(DJK-LX-2022001)of the Institute of Health and Medicine,Hefei Comprehensive National Science Centerthe Fundamental Research Funds for the Central Universities(WK9100000051).
文摘Deactivation of the mitochondrial pyruvate dehydrogenase complex(PDC)is important for the metabolic switching of cancer cell from oxidative phosphorylation to aerobic glycolysis.Studies examining PDC activity regulation have mainly focused on the phosphorylation of pyruvate dehydrogenase(E1),leaving other post-translational modifications largely unexplored.Here,we demonstrate that the acetylation of Lys 488 of pyruvate dehydrogenase complex component X(PDHX)commonly occurs in hepatocellular carcinoma,disrupting PDC assembly and contributing to lactate-driven epigenetic control of gene expression.PDHX,an E3-binding protein in the PDC,is acetylated by the p300 at Lys 488,impeding the interaction between PDHX and dihydrolipoyl transacetylase(E2),thereby disrupting PDC assembly to inhibit its activation.PDC disruption results in the conversion of most glucose to lactate,contributing to the aerobic glycolysis and H3K56 lactylation-mediated gene expression,facilitating tumor progression.These findings highlight a previously unrecognized role of PDHX acetylation in regulating PDC assembly and activity,linking PDHX Lys 488 acetylation and histone lactylation during hepatocellular carcinoma progression and providing a potential biomarker and therapeutic target for further development.
