This study focused on enhancing the production of gamma-aminobutyric acid(GABA)by Lactiplantibacillus plantarum using ultrasound-assisted fermentation.L.plantarum with high GABA-producing capacity was isolated from Ch...This study focused on enhancing the production of gamma-aminobutyric acid(GABA)by Lactiplantibacillus plantarum using ultrasound-assisted fermentation.L.plantarum with high GABA-producing capacity was isolated from Chinese pickles,and the optimized ultrasonic conditions were achieved after 9 h of seed inoculation at 28 kHz for 40 min.Compared with the control,sonication significantly increased the GABA yield by 29.72%(P<0.05).The kinetic model revealed an increase in the maximum viable cell count from 1.42 to 1.63×10^(8)CFU/mL following sonication.Scanning electron microscopy(SEM)revealed bacterial morphological changes,which might have increased the cell surface area.Sonication also increased the activities of adenosine triphosphatase(ATPase)and alkaline phosphatase(AKP),as well as the conductivity of fermentation broth,by 176.8%,21.58%,and 1.57%,respectively.Furthermore,the activity of glycolysis-related enzymes improved significantly after ultrasonic treatment.Images of inverted fluorescence microscopy indicated a substantial increase in the intracellular Ca^(2+)concentration.The 1,6-diphenyl-1,3,5-hexanetriene(DPH)fluorescent probe analysis showed an increase in cell membrane fluidity.Both of the above results were associated with increased membrane permeability.These findings suggest that sonication may increase GABA production by promoting bacterial growth and metabolic activity,as well as improving cell permeability,making it a promising approach to improve the nutritional value of fermented products.展开更多
The atmospheric and room temperature plasma(ARTP)technique efficiently enhances yeast glutathione(GSH)yield.This study focused on obtaining a GSH over-producing strain,Saccharomyces cerevisiae SCZ40,through mutagenesi...The atmospheric and room temperature plasma(ARTP)technique efficiently enhances yeast glutathione(GSH)yield.This study focused on obtaining a GSH over-producing strain,Saccharomyces cerevisiae SCZ40,through mutagenesis and stability testing.SCZ40 exhibited a remarkable 71.1%increase in GSH content during rice wine fermentation.Scanning electron microscopy(SEM)and whole-genome sequencing identified ARTP-induced changes in cell morphology and alterations in the protein-serine/threonine kinase and isocitrate dehydrogenase pathways linked to GSH synthesis.Transcriptomic analysis revealed 507 differentially expressed genes,highlighting the impact of ARTP on S.cerevisiae.Up/down-regulated genes associated with glycolysis/gluconeogenesis suggested a shift in glucose distribution favoring the pentose phosphate pathway and positively influencing GSH production.Pathway analysis further elucidated changes in glycolysis/gluconeogenesis,tricarboxylic acid cycle(TCA),and GSH biosynthesis pathways.Validation through quantitative real-time polymerase chain reaction(qRT-PCR)confirmed RNA-seq data reliability.展开更多
基金supported by grants from the Primary Research&Development Plan of Jiangsu Province(BE2020329)sponsored by a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘This study focused on enhancing the production of gamma-aminobutyric acid(GABA)by Lactiplantibacillus plantarum using ultrasound-assisted fermentation.L.plantarum with high GABA-producing capacity was isolated from Chinese pickles,and the optimized ultrasonic conditions were achieved after 9 h of seed inoculation at 28 kHz for 40 min.Compared with the control,sonication significantly increased the GABA yield by 29.72%(P<0.05).The kinetic model revealed an increase in the maximum viable cell count from 1.42 to 1.63×10^(8)CFU/mL following sonication.Scanning electron microscopy(SEM)revealed bacterial morphological changes,which might have increased the cell surface area.Sonication also increased the activities of adenosine triphosphatase(ATPase)and alkaline phosphatase(AKP),as well as the conductivity of fermentation broth,by 176.8%,21.58%,and 1.57%,respectively.Furthermore,the activity of glycolysis-related enzymes improved significantly after ultrasonic treatment.Images of inverted fluorescence microscopy indicated a substantial increase in the intracellular Ca^(2+)concentration.The 1,6-diphenyl-1,3,5-hexanetriene(DPH)fluorescent probe analysis showed an increase in cell membrane fluidity.Both of the above results were associated with increased membrane permeability.These findings suggest that sonication may increase GABA production by promoting bacterial growth and metabolic activity,as well as improving cell permeability,making it a promising approach to improve the nutritional value of fermented products.
基金supported by grants from the National Natural Science Foundation of China(31972208)the National Primary Research&Development Plan of China(2016YFD0401401)+1 种基金Zhenjiang“1+1+”New Agricultural Technology Extension Project(ZJNJ[2019]04)sponsored by a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘The atmospheric and room temperature plasma(ARTP)technique efficiently enhances yeast glutathione(GSH)yield.This study focused on obtaining a GSH over-producing strain,Saccharomyces cerevisiae SCZ40,through mutagenesis and stability testing.SCZ40 exhibited a remarkable 71.1%increase in GSH content during rice wine fermentation.Scanning electron microscopy(SEM)and whole-genome sequencing identified ARTP-induced changes in cell morphology and alterations in the protein-serine/threonine kinase and isocitrate dehydrogenase pathways linked to GSH synthesis.Transcriptomic analysis revealed 507 differentially expressed genes,highlighting the impact of ARTP on S.cerevisiae.Up/down-regulated genes associated with glycolysis/gluconeogenesis suggested a shift in glucose distribution favoring the pentose phosphate pathway and positively influencing GSH production.Pathway analysis further elucidated changes in glycolysis/gluconeogenesis,tricarboxylic acid cycle(TCA),and GSH biosynthesis pathways.Validation through quantitative real-time polymerase chain reaction(qRT-PCR)confirmed RNA-seq data reliability.
