Formaldehyde is a well-known carcinogen that can occur naturally or be illicitly added as an adulterant to food,calling for rapid and reliable detection methods.In this work,leveraging the facile and selective transfo...Formaldehyde is a well-known carcinogen that can occur naturally or be illicitly added as an adulterant to food,calling for rapid and reliable detection methods.In this work,leveraging the facile and selective transformation of formaldehyde into 5-nitro-benzimidazole(5-nitro-BZM)using 4-nitro-o-phenylenediamine,two haptens were designed to target the BZM structure,leading to the production of a monoclonal antibody specifically recognizing BZM.Utilizing this antibody,a lateral flow immunoassay(LFIA)was developed for the detection of formaldehyde in grass carp,bighead carp,and basal shrimp,following a rapid pre-analysis derivatization.The detection limits were 0.15 mg/kg in grass carp,0.11 mg/kg in bighead carp,and 0.14 mg/kg in kiwi shrimp.The recoveries were in the range from 75.3%to 89.2%,and the LFIA results showed good correlation with those obtained from the HPLC.This marks the first report concerning an immunoassay for formaldehyde,offering a more rapid and cost-effective alternative for routine formaldehyde monitoring.展开更多
基金supported by the National Nature Science Foundation of China(32472423)the Guangdong Basic and Applied Basic Research Foundation(2023A1515030011)+1 种基金the“Climbing Plan”Project of Guangdong College Students Science and Technology Innovation Cultivation Special Fund(pdih2023b0386)College Student Innovation and Entrepreneurship Training Program Project of South China Agricultural University(202310564023).
文摘Formaldehyde is a well-known carcinogen that can occur naturally or be illicitly added as an adulterant to food,calling for rapid and reliable detection methods.In this work,leveraging the facile and selective transformation of formaldehyde into 5-nitro-benzimidazole(5-nitro-BZM)using 4-nitro-o-phenylenediamine,two haptens were designed to target the BZM structure,leading to the production of a monoclonal antibody specifically recognizing BZM.Utilizing this antibody,a lateral flow immunoassay(LFIA)was developed for the detection of formaldehyde in grass carp,bighead carp,and basal shrimp,following a rapid pre-analysis derivatization.The detection limits were 0.15 mg/kg in grass carp,0.11 mg/kg in bighead carp,and 0.14 mg/kg in kiwi shrimp.The recoveries were in the range from 75.3%to 89.2%,and the LFIA results showed good correlation with those obtained from the HPLC.This marks the first report concerning an immunoassay for formaldehyde,offering a more rapid and cost-effective alternative for routine formaldehyde monitoring.
