Historical insights into medicine and food homology(MFH)Evolution of the MFH theory MFH,a basic concept in traditional Chinese medicine(TCM),suggests that many materials function as both food and medicine,providing no...Historical insights into medicine and food homology(MFH)Evolution of the MFH theory MFH,a basic concept in traditional Chinese medicine(TCM),suggests that many materials function as both food and medicine,providing nourishment and simultaneously treating ailments.This concept has evolved significantly throughout TCM history^([1]).Integral to this evolution was the discovery of certain materials with dual purposes during early searches for food.These materials not only offer sustenance and nutrition but are also capable of treating simple illnesses^([2]).展开更多
XueBiJing is an intravenous five-herb injection used to treat sepsis in China.The study aimed to develop a liquid chromatography-tandem mass spectrometry(LC-MS/MS)-or liquid chromatography-ultraviolet(LC-UV)-based ass...XueBiJing is an intravenous five-herb injection used to treat sepsis in China.The study aimed to develop a liquid chromatography-tandem mass spectrometry(LC-MS/MS)-or liquid chromatography-ultraviolet(LC-UV)-based assay for quality evaluation of XueBiJing.Assay development involved identifying marker constituents to make the assay therapeutically relevant and building a reliable one-point calibrator for monitoring the various analytes in parallel.Nine marker constituents from the five herbs were selected based on XueBiJing's chemical composition,pharmacokinetics,and pharmacodynamics.A selectivity test(for“similarity of response”)was developed to identify and minimize interference by nontarget constituents.Then,an intercept test was developed to fulfill“linearity through zero”for each analyte(absolute ratio of intercept to C response,<2%).Using the newly developed assays,we analyzed samples from 33 batches of XueBiJing,manufactured over three years,and found small batch-to-batch variability in contents of the marker constituents(4.1%-14.8%),except for senkyunolide I(26.5%).展开更多
Stress has been suggested to disturb the 5-hydroxytryptamine system and decrease neurogenesis, which contribute to the development of depression. Few studies have investigated the effect of predator stress, a type of ...Stress has been suggested to disturb the 5-hydroxytryptamine system and decrease neurogenesis, which contribute to the development of depression. Few studies have investigated the effect of predator stress, a type of psychological stress, on depression and hippocampal neurogenesis in adult mice; we therefore investigated this in the present study. A total of 35 adult male Kunming mice were allocated to a cat stress group, cat odor stress group, cat stress + fluoxetine group, cat odor stress + fluoxetine group, or a control group(no stress/treatment). After 12 days of cat stress or cat odor stress, behavioral correlates of depression were measured using the open field test, elevated plus maze test, and dark-avoidance test. The concentrations of hippocampal 5-hydroxytryptamine and 5-hydroxyindoleacetic acid were measured using high-performance liquid chromatography-electrochemical detection. Neurogenesis was also analyzed using a bromodeoxyuridine and doublecortin double-immunostaining method. Cat stress and cat odor stress induced depression-like behaviors; this effect was stronger in the cat stress model. Furthermore, compared with the control group, cat stress mice exhibited lower 5-hydroxytryptamine concentrations, higher 5-hydroxyindoleacetic acid concentrations, and significantly fewer bromodeoxyuridine+/doublecortin+-labeled cells in the dentate gyrus, which was indicative of less neurogenesis. The changes observed in the cat stress group were not seen in the cat stress + fluoxetine group, which suggests that the effects of predator stress on depression and neurogenesis were reversed by fluoxetine. Taken together, our results indicate that depression-like behaviors induced by predator stress are associated with the inhibition of hippocampal neurogenesis.展开更多
Objective:Xiaoyao san(XYS)is a classic traditional Chinese medicinal formula.It has been clinically administered to regulate liver function.However,its mechanisms in glucocorticoid-induced hepatic steatosis are unknow...Objective:Xiaoyao san(XYS)is a classic traditional Chinese medicinal formula.It has been clinically administered to regulate liver function.However,its mechanisms in glucocorticoid-induced hepatic steatosis are unknown.This study aimed to investigate whether XYS protects against corticosterone(CORT)-induced hepatic steatosis,and to explore its mechanism.Methods:High-fat diet mice induced with hepatic steatosis by 2mg/kg CORT were administered 2.56 g/kg or 5.12 g/kg XYS daily for 7 weeks.The effects of XYS on hepatic steatosis in mice were evaluated by H&E and Oil Red O staining and by measuring their plasma lipids(triglyceride,total cholesterol,and free fatty acids).The mechanism of XYS against hepatic steatosis was investigated by network pharmacology,immunohistochemistry,western blotting,and gain-of-function/loss-offunction experiments.Results:XYS alleviated CORT-induced steatosis,decreased plasma lipids,and inhibited glucocorticoid receptor(GR)activation in the liver.Network pharmacology data indicated that XYS may have mitigated hepatic steatosis via GR which mediated adipose differentiation-related protein(ADFP).Gain-of-function/loss-of-function experiments in vitro confirmed that GR positively regulated ADFP expression.Conclusions:XYS ameliorated CORT-induced hepatic steatosis by downregulating the GR/ADFP axis and inhibiting lipid metabolism.Our studies implicate that XYS is promising as a therapy for CORT-induced hepatic steatosis,and lay the foundation for designing novel prophylactic and therapeutic strategies on CORT-induced hepatic steatosis.展开更多
As a chronic disease that seriously endangers public health, the number of coronary heart disease （CHD） patients in China has increased in recent years. There is great potentiality to integrate traditional Chinese a...As a chronic disease that seriously endangers public health, the number of coronary heart disease （CHD） patients in China has increased in recent years. There is great potentiality to integrate traditional Chinese and Western medicine therapies to prevent and treat CHD in clinical practice. However, most of the current integrated therapies still lack sufficient high-quality evidence, and the key links in how to apply are unclear. It is urgent to optimize them through evidence-based research to further improve the effectiveness. Therefore, we propose strategies to conduct evidence-based optimization of integrated traditional Chinese and Western medicine therapies in prevention and treatment of progressive cardiovascular diseases. These are integrated clinical trial design, attention to the key links of taking effect, combination of clinical and basic research. Based on the strategies, we started the national key research and development project ＂Evidence-based optimization research of TCM therapies in prevention and treatment of CHD （angina pectoris - myocardial infarction - heart failure）＂, which is expected to be a new paradigm in the field.展开更多
Objective:It is well known that stress plays a critical role in immune response and susceptibility to diseases.Among many stressors,restraint stress has been shown to suppress immune function and increase susceptibili...Objective:It is well known that stress plays a critical role in immune response and susceptibility to diseases.Among many stressors,restraint stress has been shown to suppress immune function and increase susceptibility to infections.In this study,we employed a restraint-mouse model to investigate the effect and preliminary mechanism of ShiQi herbal tea(SQHT),a traditional Chinese medicine,on H1N1 virus infection.Methods:Mice were exposed to restraint stress and infected with H1N1 influenza virus by intranasal inoculation.SQHT(936 and 1872 mg/kg/d)was orally administrated to mice for 7 days from the first day of restraint stress.The survival rate of mice in each group was monitored daily for 21 days.Histopathological changes,inflammatory cells infiltration,and virus titer in lungs were examined.For the study of mechanisms,we investigated whether SQHT could promote interferon-β(IFN-β)generation and interferon stimulated genes(ISG)expression.Results:Our results suggested that SQHT(936 mg/kg/d)significantly reduced H1N1-induced mortality,the level of complement C5a and lung tissue inflammation,and viral replication in restraint-stressed mice.Further results revealed that in restraint-stressed mice model,SQHT(936 mg/kg/d)administration markedly improved IFN-βgeneration,and increased MX1 and IFITM3 gene expression.Conclusion:Our study demonstrates that SQHT reduces restraint stress-induced susceptibility to H1N1 infection via improving IFN-βantiviral pathway,which provides a certain basis for the clinical use of SQHT to treat H1N1 infection.展开更多
Objective： Mangiferin （MF） is a polyphenol isolated from the root of Anemarrhena asphodeloides Bge.. This study wasaimed to investigate the effects of MF on hyperglycemia in animal models of insulin resistance and ...Objective： Mangiferin （MF） is a polyphenol isolated from the root of Anemarrhena asphodeloides Bge.. This study wasaimed to investigate the effects of MF on hyperglycemia in animal models of insulin resistance and streptozotocin（STZ）-induced diabetes. Methods： The diabetes mellitus model was established in mice by receiving a multiplehypodermic injection of hydrocortisone sodium succinate （HCSS） （70 mg/kg） or a single intravenous injection of STZ（130 mg/kg）. Meanwhile MF at different dosage （50, 100 and 200 mg/kg） were oral administrated for consecutive 10days. Data of blood glucose were collected at different time after intraperitoneal injection of insulin （0.5 U/kg） toinvestigate the insulin resistant. As well as the oxygen radical absorbance capacity （ORAC） and superoxide dismutase（SOD） activity of kidney were measured. The in vitro experiment was established to investigate the inhibitory capacity ofMF to α-glucosidase. Results： Oral administration of MF significantly prevented insulin resistance caused by HCSSinjection. STZ-induced diabetic symptoms were also improved, including fasting blood glucose, glycated hemoglobin,plasma triglycerides, hepatic glycogen, kidney SOD and ORAC level. The in vitro experiment demonstrated that MF hadpotent α-glucosidase inhibitory activity. Conclusion： The obtained results demonstrate that MF ameliorates insulinresistance and STZ-induced glucose metabolism disturbance. The MF exerts the protective effects through improving theantioxidant ability, promoting hepatic glycogen synthesis and inhibiting α-glucosidase activity.展开更多
Objective： （＋）-Clausenamide （（＋）-CLA）, the active ingredient of wampee, was isolated from the leaves of Clausena lansium （Lour.） Skeels. This study aimed to evaluate the protective potential of ...Objective： （＋）-Clausenamide （（＋）-CLA）, the active ingredient of wampee, was isolated from the leaves of Clausena lansium （Lour.） Skeels. This study aimed to evaluate the protective potential of （＋）-CLA against acetaminophen （APAP）-induced nephrotoxicity in mice. Methods： Mice were divided into control, APAP, high-dose （＋）-CLA, and low-dose （＋）-CLA groups. Then, mice were preadministered （＋）-CLA （50 and 100 mg/kg） for 5 consecutive days. After the last treatment, the animals received a single intraperitoneal injection of APAP （600 mg/kg）. Renal histopathology was evaluated by staining with hematoxylin and eosin. The levels of malondialdehyde （MDA） and glutathione （GSH） and the activities of catalase （CAT） and superoxide dismutase （SOD） were determined using corresponding kits. Western blotting was used to analyze the expression of apoptosis-related proteins in renal tissue. Results： Administration of APAP increased serum creatinine and blood urea nitrogen levels in comparison with the control group. An increase in renal MDA level, depletion of GSH, and reductions in CAT and SOD activities in renal tissue indicated that APAP-induced kidney injury was mediated by oxidative stress. The expressions of Bax and caspase-3, cleavage of caspase-3, and cytoplasm cytochrome c levels were up-regulated in renal tissue, whereas Bcl-2 expression and mitochondrial cytochrome c levels were down-regulated in the APAP group, which revealed that APAP-induced kidney injury significantly increased cell apoptosis in renal tubules. The histopathology of kidney tissue supported these biochemical mechanisms. （＋）-CLA can reverse changes in most of the abovementioned parameters and nearly restore the normal structure of the kidney. Conclusion： Oxidative stress and apoptosis are considered to be the mechanisms underlying APAP-induced nephrotoxicity. （＋）-CLA could be a promising antidote for APAP-induced acute renal damage owing to its antioxidative and antiapoptotic effects.展开更多
The metabolic state of an organism reflects both external environmental factors and internal genetic influences, providing insights into physiological conditions and potential disease mechanisms. Historically, our und...The metabolic state of an organism reflects both external environmental factors and internal genetic influences, providing insights into physiological conditions and potential disease mechanisms. Historically, our understanding of organismal metabolism was relatively limited by technological constraints. With the rapid advancement of mass spectrometrybased methods and strategies for studying endogenous small molecules.展开更多
Ovarian tumor(OT)is the most lethal form of gynecologic malignancy,with minimal improvements in patient outcomes over the past several decades.Metastasis is the leading cause of ovarian cancer-related deaths,yet the u...Ovarian tumor(OT)is the most lethal form of gynecologic malignancy,with minimal improvements in patient outcomes over the past several decades.Metastasis is the leading cause of ovarian cancer-related deaths,yet the underlying mechanisms remain poorly understood.Psychological stress is known to activate the glucocorticoid receptor(NR3C1),a factor associated with poor prognosis in OT patients.However,the precise mechanisms linking NR3C1 signaling and metastasis have yet to be fully elucidated.In this study,we demonstrate that chronic restraint stress accelerates epithelialemesenchymal transition(EMT)and metastasis in OT through an NR3C1-dependent mechanism involving nuclear protein 1(NUPR1).Mechanistically,NR3C1 directly regulates the transcription of NUPR1,which in turn increases the expression of snail family transcriptional repressor 2(SNAI2),a key driver of EMT.Clinically,elevated NR3C1 positively correlates with NUPR1 expression in OT patients,and both are positively associated with poorer prognosis.Overall,our study identified the NR3C1/NUPR1 axis as a critical regulatory pathway in psychological stress-induced OT metastasis,suggesting a potential therapeutic target for intervention in OT metastasis.展开更多
Pyroptosis typically features the perforation of cell membranes,a process triggered by the exposure of active N-terminal domain of Gasdermin D(GSDMD).This pivotal event subsequently leads to the release of pro-inflamm...Pyroptosis typically features the perforation of cell membranes,a process triggered by the exposure of active N-terminal domain of Gasdermin D(GSDMD).This pivotal event subsequently leads to the release of pro-inflammatory cytokines,notably IL-1a and IL-1b,thereby fostering tissue inflammation and impeding the repair process 1.Moreover,the question of whether small molecule metabolites expelled through these pyroptotic pores possess specific physiological functions has consistently captivated the scientific community’s curiosity.A recent study published in Nature by Mehrotra et al.2 unveiled a novel paradigm,suggesting that oxygenated polyunsaturated fatty acids(oxylipins),released from pyroptotic cells,can paradoxically promote wound healing.This research demonstrates,as the famous saying goes,“From death comes rebirth”,innovatively revealing the interdependent relationship between cell death,inflammation,and repair.展开更多
Herpes simplex virus type 1(HSV-1),a neurotropic herpes virus,is able to establish a lifelong latent infection in the human host.Following primary replication in mucosal epithelial cells,the virus can enter sensory ne...Herpes simplex virus type 1(HSV-1),a neurotropic herpes virus,is able to establish a lifelong latent infection in the human host.Following primary replication in mucosal epithelial cells,the virus can enter sensory neurons innervating peripheral tissues via nerve termini.The viral genome is then transported to the nucleus where it can be maintained without producing infectious progeny,and thus latency is established in the cell.Yin-Yang balance is an essential concept in traditional Chinese medicine(TCM)theory.Yin represents stable and inhibitory factors,and Yang represents the active and aggressive factors.When the organism is exposed to stress,especially psychological stress caused by emotional stimulation,the Yin-Yang balance is disturbed and the virus can re-engage in productive replication,resulting in recurrent diseases.Therefore,a better understanding of the stress-induced susceptibility to HSV-1 primary infection and reactivation is needed and will provide helpful insights into the effective control and treatment of HSV-1.Here we reviewed the recent advances in the studies of HSV-1 susceptibility,latency and reactivation.We included mechanisms involved in primary infection and the regulation of latency and described how stress-induced changes increase the susceptibility to primary and recurrent infections.展开更多
The relationship between chronic psychological stress and tumorigenesis has been well defined in epidemiological studies;however, the underlying mechanism remains underexplored. In this study, we discovered that impai...The relationship between chronic psychological stress and tumorigenesis has been well defined in epidemiological studies;however, the underlying mechanism remains underexplored. In this study, we discovered that impaired macrophage phagocytosis contributed to the psychological stressevoked tumor susceptibility, and the stress hormone glucocorticoid(GC) was identified as a principal detrimental factor. Mechanistically, GC disturbed the balance of the "eat me" signal receptor(low-density lipoprotein receptor-related protein-1, LRP1) and the "don’t eat me" signal receptor(signal regulatory protein alpha, SIRPa). Further analysis revealed that GC led to a direct, glucocorticoid receptor(GR)-dependent trans-repression of LRP1 expression, and the repressed LRP1, in turn, resulted in the elevatedgene level of SIRPa by down-regulating mi RNA-4695-3 p. These data collectively demonstrate that stress induces the imbalance of the LRP1/SIRPa axis and entails the disturbance of tumor cell clearance by macrophages. Our findings provide the mechanistic insight into psychological stress-evoked tumor susceptibility and indicate that the balance of LRP1/SIRPa axis may serve as a potential therapeutic strategy for tumor treatment.展开更多
Myocardial ischemia/reperfusion(/R)injury is a classic type of cardiovascular disease characterized by injury to cardiomyocytes leading to various forms of cell death.It is believed that irreversible myocardial damage...Myocardial ischemia/reperfusion(/R)injury is a classic type of cardiovascular disease characterized by injury to cardiomyocytes leading to various forms of cell death.It is believed that irreversible myocardial damage resulted from I/R occurs due to oxidative stress evoked during the reperfusion phase.Here we demonstrate that ischemia triggers a specific redox reaction of polyunsaturated fatty acids(PUFA)-phospholipids in myocardial cells,which acts as a priming signaling that initiates the outbreak of robust oxidative damage in the reperfusion phase.Using animal and in vitro models,the crucial lipid species in I/R injury were identifed to be oxidized PUFAs enriched phosphatidylethanolamines.Using multi-omics,arachidonic acid 15-lipoxygenase-1(ALOx15)was identified as the primary mediator of ischemia-provoked phospholipid peroxidation,which was further confirmed using chemogenetic approaches.Collectively,our results reveal that ALOx15 induction in the ischemia phase acts as a"burning point"to ignite phospholipid oxidization into ferroptotic signals.This finding characterizes a novel molecular mechanism for myocardial ischemia injury and offers a potential therapeutic target for early intervention of V/R injury.展开更多
Herpes simplex virus type 1(HSV-1)is a ubiquitous and widespread human pathogen,which gives rise to a range of diseases,including cold sores,corneal blindness,and encephalitis.Currently,the use of nucleoside analogs,s...Herpes simplex virus type 1(HSV-1)is a ubiquitous and widespread human pathogen,which gives rise to a range of diseases,including cold sores,corneal blindness,and encephalitis.Currently,the use of nucleoside analogs,such as acyclovir and penciclovir,in treating HSV-1 infection often presents limitation due to their side effects and low efficacy for drug-resistance strains.Therefore,new anti-herpetic drugs and strategies should be urgently developed.Here,we reported that baicalein,a naturally derived compound widely used in Asian countries,strongly inhibited HSV-1 replication in several models.Baicalein was effective against the replication of both HSV-1/F and HSV-1/Blue(an acyclovir-resistant strain)in vitro.In the ocular inoculation mice model,baicalein markedly reduced in vivo HSV-1/F replication,receded inflammatory storm and attenuated histological changes in the cornea.Consistently,baicalein was found to reduce the mortality of mice,viral loads both in nose and trigeminal ganglia in HSV-1 intranasal infection model.Moreover,an ex vivo HSV-1-EGFP infection model established in isolated murine epidermal sheets confirmed that baicalein suppressed HSV-1 replication.Further investigations unraveled that dual mechanisms,inactivating viral particles and inhibiting IκB kinase beta(IKK-β)phosphorylation,were involved in the anti-HSV-1 effect of baicalein.Collectively,our findings identified baicalein as a promising therapy candidate against the infection of HSV-1,especially acyclovir-resistant strain.展开更多
“Life is pleasant. Death is peaceful. It's the transition that's troublesome.”——Isaac Asimov Copper is an essential trace element central to cardiovascular, neuronal, and immune functions. While copper def...“Life is pleasant. Death is peaceful. It's the transition that's troublesome.”——Isaac Asimov Copper is an essential trace element central to cardiovascular, neuronal, and immune functions. While copper deficiency is generally rare, chronic or long-term copper exposure can result in toxicity.展开更多
Although stress has been known to increase the susceptibility of pathogen infection,the underlying mechanism remains elusive.In this study,we reported that restraint stress dramatically enhanced the morbidity and mort...Although stress has been known to increase the susceptibility of pathogen infection,the underlying mechanism remains elusive.In this study,we reported that restraint stress dramatically enhanced the morbidity and mortality of mice infected with the influenza virus(H1N1)and obviously aggravated lung inflammation.Corticosterone(CORT),a main type of glucocorticoids in rodents,was secreted in the plasma of stressed mice.We further found that this stress hormone significantly boosted virus replication by restricting mitochondrial antiviral signaling(MAVS)protein-transduced IFN-βproduction without affecting its mRNA level,while the deficiency of MAVS abrogated stress/CORT-induced viral susceptibility in mice.Mechanistically,the effect of CORT was mediated by proteasome-dependent degradation of MAVS,thereby resulting in the impediment of MAVS-transduced IFN-βgeneration in vivo and in vitro.Furthermore,RNA-seq assay results indicated the involvement of Mitofusin 2(Mfn2)in this process.Gain-and loss-offunction experiments indicated that Mfn2 interacted with MAVS and recruited E3 ligase SYVN1 to promote the polyubiquitination of MAVS.Co-immunoprecipitation experiments clarified an interaction between any two regions of Mfn2(HR1),MAVS(C-terminal/TM)and SYVN1(TM).Collectively,our findings define the Mfn2-SYVN1 axis as a new signaling cascade for proteasome-dependent degradation of MAVS and a‘fine tuning’of antiviral innate immunity in response to influenza infection under stress.展开更多
Four new dicaffeoylspermidine derivatives, lycibarbarspermidines P–S(1–4), were isolated from the fruit of Lycium barbarum(wolfberry).The structures were determined by extensive spectroscopic(HRESIMS, 1 D NMR, and 2...Four new dicaffeoylspermidine derivatives, lycibarbarspermidines P–S(1–4), were isolated from the fruit of Lycium barbarum(wolfberry).The structures were determined by extensive spectroscopic(HRESIMS, 1 D NMR, and 2 D NMR) analyses and chemical methods.Dicaffeoylspermidine derivatives are a kind of major bioactive and characteristic constituents in wolfberry, and the discovery of 1–4 added new members of this family.展开更多
Lipids are the major components of cells in the central nervous system(CNS) and are essential for the brain development and homeostasis. Several studies have demonstrated that impaired lipid metabolism is closely rela...Lipids are the major components of cells in the central nervous system(CNS) and are essential for the brain development and homeostasis. Several studies have demonstrated that impaired lipid metabolism is closely related to neurological diseases among the elderly^(1). Lipidomics can serve us well in identifying and quantifying lipid molecules in cells, tissues, and organisms at the system level. However, finding out biologically meaningful lipids from the massive lipidomics data is not straightforward.展开更多
It is commonly known that psychological stress affects immunity through the nervous system,but the specific stress networks between brain and peripheral leukocytes,or their relationship with disease initiation or prog...It is commonly known that psychological stress affects immunity through the nervous system,but the specific stress networks between brain and peripheral leukocytes,or their relationship with disease initiation or progression have yet to been delineated.A recent study by Poller and his colleagues1 propose an innovative perspective that distinct brain circuits rapidly manipulate a large-scale whole-body re-distribution of leukocytes(neutrophils,monocytes,lymphocytes)during psychological stress.展开更多
基金supported,in part,by Natural Science Foundation of China(82274403,T2341004,82321004,82174054)Guangdong Basic and Applied Basic Research Foundation(2023B1515020020,2023B1515040016,2021B1515120023)+4 种基金Guangzhou Basic and Applied Basic Research Foundation(2024A04J6406)China Association of Chinese Medicine(CACM-2022-QNRC2-B08)K.C.Wong Education Foundation,the Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(2017BT01Y036),GDUPS(2019),Innovation Team Project of Guangdong Provincial Department of Education(2020KCXTD003)Guangzhou Key Laboratory of Traditional Chinese Medicine&Disease Susceptibility(2024A03J090)Guangdong-Hong Kong-Macao Universities Joint Laboratory for the Internationalization of Traditional Chinese Medicine(2023LSYS002).
文摘Historical insights into medicine and food homology(MFH)Evolution of the MFH theory MFH,a basic concept in traditional Chinese medicine(TCM),suggests that many materials function as both food and medicine,providing nourishment and simultaneously treating ailments.This concept has evolved significantly throughout TCM history^([1]).Integral to this evolution was the discovery of certain materials with dual purposes during early searches for food.These materials not only offer sustenance and nutrition but are also capable of treating simple illnesses^([2]).
基金funded by grants from the National Natural Science Foundation of China(Grant Nos.:82074176 and 81503345)Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine(Grant No.:ZYYCXTD-C-202009)the National Key R&D Program of China(Grant No.:2018YFC1704500).
文摘XueBiJing is an intravenous five-herb injection used to treat sepsis in China.The study aimed to develop a liquid chromatography-tandem mass spectrometry(LC-MS/MS)-or liquid chromatography-ultraviolet(LC-UV)-based assay for quality evaluation of XueBiJing.Assay development involved identifying marker constituents to make the assay therapeutically relevant and building a reliable one-point calibrator for monitoring the various analytes in parallel.Nine marker constituents from the five herbs were selected based on XueBiJing's chemical composition,pharmacokinetics,and pharmacodynamics.A selectivity test(for“similarity of response”)was developed to identify and minimize interference by nontarget constituents.Then,an intercept test was developed to fulfill“linearity through zero”for each analyte(absolute ratio of intercept to C response,<2%).Using the newly developed assays,we analyzed samples from 33 batches of XueBiJing,manufactured over three years,and found small batch-to-batch variability in contents of the marker constituents(4.1%-14.8%),except for senkyunolide I(26.5%).
基金supported by the National Natural Science Foundation of China,No.81473115(to RRH),81622050(to RRH)the Natural Science Foundation of Guangdong Province of China for Distinguished Young Scholars,No.2017A030306004(to YFL)the Youth Top-Notch Talent Support Program of Guangdong Province of China,No.2016TQ03R586(to YFL)
文摘Stress has been suggested to disturb the 5-hydroxytryptamine system and decrease neurogenesis, which contribute to the development of depression. Few studies have investigated the effect of predator stress, a type of psychological stress, on depression and hippocampal neurogenesis in adult mice; we therefore investigated this in the present study. A total of 35 adult male Kunming mice were allocated to a cat stress group, cat odor stress group, cat stress + fluoxetine group, cat odor stress + fluoxetine group, or a control group(no stress/treatment). After 12 days of cat stress or cat odor stress, behavioral correlates of depression were measured using the open field test, elevated plus maze test, and dark-avoidance test. The concentrations of hippocampal 5-hydroxytryptamine and 5-hydroxyindoleacetic acid were measured using high-performance liquid chromatography-electrochemical detection. Neurogenesis was also analyzed using a bromodeoxyuridine and doublecortin double-immunostaining method. Cat stress and cat odor stress induced depression-like behaviors; this effect was stronger in the cat stress model. Furthermore, compared with the control group, cat stress mice exhibited lower 5-hydroxytryptamine concentrations, higher 5-hydroxyindoleacetic acid concentrations, and significantly fewer bromodeoxyuridine+/doublecortin+-labeled cells in the dentate gyrus, which was indicative of less neurogenesis. The changes observed in the cat stress group were not seen in the cat stress + fluoxetine group, which suggests that the effects of predator stress on depression and neurogenesis were reversed by fluoxetine. Taken together, our results indicate that depression-like behaviors induced by predator stress are associated with the inhibition of hippocampal neurogenesis.
基金the National Natural Science Foundation of China(Nos.81630104 and 81622050).
文摘Objective:Xiaoyao san(XYS)is a classic traditional Chinese medicinal formula.It has been clinically administered to regulate liver function.However,its mechanisms in glucocorticoid-induced hepatic steatosis are unknown.This study aimed to investigate whether XYS protects against corticosterone(CORT)-induced hepatic steatosis,and to explore its mechanism.Methods:High-fat diet mice induced with hepatic steatosis by 2mg/kg CORT were administered 2.56 g/kg or 5.12 g/kg XYS daily for 7 weeks.The effects of XYS on hepatic steatosis in mice were evaluated by H&E and Oil Red O staining and by measuring their plasma lipids(triglyceride,total cholesterol,and free fatty acids).The mechanism of XYS against hepatic steatosis was investigated by network pharmacology,immunohistochemistry,western blotting,and gain-of-function/loss-offunction experiments.Results:XYS alleviated CORT-induced steatosis,decreased plasma lipids,and inhibited glucocorticoid receptor(GR)activation in the liver.Network pharmacology data indicated that XYS may have mitigated hepatic steatosis via GR which mediated adipose differentiation-related protein(ADFP).Gain-of-function/loss-of-function experiments in vitro confirmed that GR positively regulated ADFP expression.Conclusions:XYS ameliorated CORT-induced hepatic steatosis by downregulating the GR/ADFP axis and inhibiting lipid metabolism.Our studies implicate that XYS is promising as a therapy for CORT-induced hepatic steatosis,and lay the foundation for designing novel prophylactic and therapeutic strategies on CORT-induced hepatic steatosis.
文摘As a chronic disease that seriously endangers public health, the number of coronary heart disease （CHD） patients in China has increased in recent years. There is great potentiality to integrate traditional Chinese and Western medicine therapies to prevent and treat CHD in clinical practice. However, most of the current integrated therapies still lack sufficient high-quality evidence, and the key links in how to apply are unclear. It is urgent to optimize them through evidence-based research to further improve the effectiveness. Therefore, we propose strategies to conduct evidence-based optimization of integrated traditional Chinese and Western medicine therapies in prevention and treatment of progressive cardiovascular diseases. These are integrated clinical trial design, attention to the key links of taking effect, combination of clinical and basic research. Based on the strategies, we started the national key research and development project ＂Evidence-based optimization research of TCM therapies in prevention and treatment of CHD （angina pectoris - myocardial infarction - heart failure）＂, which is expected to be a new paradigm in the field.
基金This work was financially supported,in part,by Science and Technology Project of Zhongshan(2013A3FC0253)Scientific Research Team Training Project in University of Electronic Science and Technology of China,Zhongshan Institute(No.412YT02)Natural Science Foundation of Guangdong Province-the Ph.D.Startup and Vertical Collaboration Project(2018030310186).
文摘Objective:It is well known that stress plays a critical role in immune response and susceptibility to diseases.Among many stressors,restraint stress has been shown to suppress immune function and increase susceptibility to infections.In this study,we employed a restraint-mouse model to investigate the effect and preliminary mechanism of ShiQi herbal tea(SQHT),a traditional Chinese medicine,on H1N1 virus infection.Methods:Mice were exposed to restraint stress and infected with H1N1 influenza virus by intranasal inoculation.SQHT(936 and 1872 mg/kg/d)was orally administrated to mice for 7 days from the first day of restraint stress.The survival rate of mice in each group was monitored daily for 21 days.Histopathological changes,inflammatory cells infiltration,and virus titer in lungs were examined.For the study of mechanisms,we investigated whether SQHT could promote interferon-β(IFN-β)generation and interferon stimulated genes(ISG)expression.Results:Our results suggested that SQHT(936 mg/kg/d)significantly reduced H1N1-induced mortality,the level of complement C5a and lung tissue inflammation,and viral replication in restraint-stressed mice.Further results revealed that in restraint-stressed mice model,SQHT(936 mg/kg/d)administration markedly improved IFN-βgeneration,and increased MX1 and IFITM3 gene expression.Conclusion:Our study demonstrates that SQHT reduces restraint stress-induced susceptibility to H1N1 infection via improving IFN-βantiviral pathway,which provides a certain basis for the clinical use of SQHT to treat H1N1 infection.
文摘Objective： Mangiferin （MF） is a polyphenol isolated from the root of Anemarrhena asphodeloides Bge.. This study wasaimed to investigate the effects of MF on hyperglycemia in animal models of insulin resistance and streptozotocin（STZ）-induced diabetes. Methods： The diabetes mellitus model was established in mice by receiving a multiplehypodermic injection of hydrocortisone sodium succinate （HCSS） （70 mg/kg） or a single intravenous injection of STZ（130 mg/kg）. Meanwhile MF at different dosage （50, 100 and 200 mg/kg） were oral administrated for consecutive 10days. Data of blood glucose were collected at different time after intraperitoneal injection of insulin （0.5 U/kg） toinvestigate the insulin resistant. As well as the oxygen radical absorbance capacity （ORAC） and superoxide dismutase（SOD） activity of kidney were measured. The in vitro experiment was established to investigate the inhibitory capacity ofMF to α-glucosidase. Results： Oral administration of MF significantly prevented insulin resistance caused by HCSSinjection. STZ-induced diabetic symptoms were also improved, including fasting blood glucose, glycated hemoglobin,plasma triglycerides, hepatic glycogen, kidney SOD and ORAC level. The in vitro experiment demonstrated that MF hadpotent α-glucosidase inhibitory activity. Conclusion： The obtained results demonstrate that MF ameliorates insulinresistance and STZ-induced glucose metabolism disturbance. The MF exerts the protective effects through improving theantioxidant ability, promoting hepatic glycogen synthesis and inhibiting α-glucosidase activity.
基金This work was partly supported by TCM Modernization Program of Hainan Province （2015ZY01）, Natural Science Foundation of China （81573675 ＆ 81622050 ＆ 81673709）, Science and Technology Program of Guangzhou （201604046016 ＆ 201604020175） and Research Project of Guangdong Provincial Bureau of Traditional Chinese Medicine （20161070）.
文摘Objective： （＋）-Clausenamide （（＋）-CLA）, the active ingredient of wampee, was isolated from the leaves of Clausena lansium （Lour.） Skeels. This study aimed to evaluate the protective potential of （＋）-CLA against acetaminophen （APAP）-induced nephrotoxicity in mice. Methods： Mice were divided into control, APAP, high-dose （＋）-CLA, and low-dose （＋）-CLA groups. Then, mice were preadministered （＋）-CLA （50 and 100 mg/kg） for 5 consecutive days. After the last treatment, the animals received a single intraperitoneal injection of APAP （600 mg/kg）. Renal histopathology was evaluated by staining with hematoxylin and eosin. The levels of malondialdehyde （MDA） and glutathione （GSH） and the activities of catalase （CAT） and superoxide dismutase （SOD） were determined using corresponding kits. Western blotting was used to analyze the expression of apoptosis-related proteins in renal tissue. Results： Administration of APAP increased serum creatinine and blood urea nitrogen levels in comparison with the control group. An increase in renal MDA level, depletion of GSH, and reductions in CAT and SOD activities in renal tissue indicated that APAP-induced kidney injury was mediated by oxidative stress. The expressions of Bax and caspase-3, cleavage of caspase-3, and cytoplasm cytochrome c levels were up-regulated in renal tissue, whereas Bcl-2 expression and mitochondrial cytochrome c levels were down-regulated in the APAP group, which revealed that APAP-induced kidney injury significantly increased cell apoptosis in renal tubules. The histopathology of kidney tissue supported these biochemical mechanisms. （＋）-CLA can reverse changes in most of the abovementioned parameters and nearly restore the normal structure of the kidney. Conclusion： Oxidative stress and apoptosis are considered to be the mechanisms underlying APAP-induced nephrotoxicity. （＋）-CLA could be a promising antidote for APAP-induced acute renal damage owing to its antioxidative and antiapoptotic effects.
基金supported by the National Natural Science Foundation of China (T2341004,82274403,82321004, 82174054)the National Key Research and Development Program of China (2024YFC3507200,2024YFA0919800)+7 种基金Guangdong Basic and Applied Basic Research Foundation (2023B1515020020,2023B1515040016, 2021B1515120023)Guangdong Major Project of Basic and Applied Basic Research (2023B0303000013)Guangzhou Basic and Applied Basic Research Foundation (2024A04J6406)China Association of Chinese Medicine (CACM-2022-QNRC2-B08)K.C.Wong Education FoundationInnovation Team Project of Guangdong Provincial Department of Education (2020KCXTD003)Guangzhou Key Laboratory of Traditional Chinese Medicine & Disease Susceptibility (2024A03J090)Guangdong-Hong Kong-Macao Universities Joint Laboratory for the Internationalization of Traditional Chinese Medicine (2023LSYS002)。
文摘The metabolic state of an organism reflects both external environmental factors and internal genetic influences, providing insights into physiological conditions and potential disease mechanisms. Historically, our understanding of organismal metabolism was relatively limited by technological constraints. With the rapid advancement of mass spectrometrybased methods and strategies for studying endogenous small molecules.
基金supported,in part,by the National Natural Science Foundation of China(Nos.82374327,81973718,T2341004,and 82321004)Natural Science Foundation of Guangdong Province(Nos.2021A1515011297,2023B1515040016,2019A1515110952and 2022A1515012240,China)+7 种基金the Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(No.2017BT01Y036,China)Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme(2019,China)Guangzhou Basic and Applied Basic Research Foundation(No.2024A04J4020,China)Science and Technology Program of Guangzhou(Nos.202201020483 and 202201010786,China)Foundation of Guangdong Second Provincial General Hospital(No.3DA2021015,China)Innovation Team Project of Guangdong Provincial Department of Education(No.2020KCXTD003,China)GuangdongeHong KongeMacao Universities Joint Laboratory for the Internationalization of Traditional Chinese Medicine(No.2023LSYS002,China)Guangzhou Key Laboratory of Traditional Chinese Medicine&Disease Susceptibility(No.2024A03J090,China).
文摘Ovarian tumor(OT)is the most lethal form of gynecologic malignancy,with minimal improvements in patient outcomes over the past several decades.Metastasis is the leading cause of ovarian cancer-related deaths,yet the underlying mechanisms remain poorly understood.Psychological stress is known to activate the glucocorticoid receptor(NR3C1),a factor associated with poor prognosis in OT patients.However,the precise mechanisms linking NR3C1 signaling and metastasis have yet to be fully elucidated.In this study,we demonstrate that chronic restraint stress accelerates epithelialemesenchymal transition(EMT)and metastasis in OT through an NR3C1-dependent mechanism involving nuclear protein 1(NUPR1).Mechanistically,NR3C1 directly regulates the transcription of NUPR1,which in turn increases the expression of snail family transcriptional repressor 2(SNAI2),a key driver of EMT.Clinically,elevated NR3C1 positively correlates with NUPR1 expression in OT patients,and both are positively associated with poorer prognosis.Overall,our study identified the NR3C1/NUPR1 axis as a critical regulatory pathway in psychological stress-induced OT metastasis,suggesting a potential therapeutic target for intervention in OT metastasis.
基金supported,in part,by Natural Science Foundation of China(T2341004,82321004,82374558,82074511,81973660)the National Key Research and Development Program of China(2022YFE0206200 and 2023YFC2414500)+1 种基金Guangdong Basic and Applied Basic Research Foundation(2021B1515120023,China)Guangzhou Basic and Applied Basic Research Foundation(2024A04J4020,China).
文摘Pyroptosis typically features the perforation of cell membranes,a process triggered by the exposure of active N-terminal domain of Gasdermin D(GSDMD).This pivotal event subsequently leads to the release of pro-inflammatory cytokines,notably IL-1a and IL-1b,thereby fostering tissue inflammation and impeding the repair process 1.Moreover,the question of whether small molecule metabolites expelled through these pyroptotic pores possess specific physiological functions has consistently captivated the scientific community’s curiosity.A recent study published in Nature by Mehrotra et al.2 unveiled a novel paradigm,suggesting that oxygenated polyunsaturated fatty acids(oxylipins),released from pyroptotic cells,can paradoxically promote wound healing.This research demonstrates,as the famous saying goes,“From death comes rebirth”,innovatively revealing the interdependent relationship between cell death,inflammation,and repair.
基金supported,in part,by the National Key Research and Development Program of China,China(grant number2017YFC1700404)Natural Science Foundation of China,China(grant numbers 81622050,81573675,81673709,81560661 and81873209)+3 种基金the Young Top-notch Talent Support Program of Guangdong Province,China(grant numbers 2014TQ01R229 and2016TQ03R586)Guangdong Province Ocean and Fisheries Bureau-Key Technology Research and Development Program,China(grant number A201701A02)Guangdong Science and Technology Foundation for Distinguished Young Scholars,China(grant number 2017A030306004)the Science and Technology Program of Guangzhou,China(grant numbers 201604046016 and201610010182).
文摘Herpes simplex virus type 1(HSV-1),a neurotropic herpes virus,is able to establish a lifelong latent infection in the human host.Following primary replication in mucosal epithelial cells,the virus can enter sensory neurons innervating peripheral tissues via nerve termini.The viral genome is then transported to the nucleus where it can be maintained without producing infectious progeny,and thus latency is established in the cell.Yin-Yang balance is an essential concept in traditional Chinese medicine(TCM)theory.Yin represents stable and inhibitory factors,and Yang represents the active and aggressive factors.When the organism is exposed to stress,especially psychological stress caused by emotional stimulation,the Yin-Yang balance is disturbed and the virus can re-engage in productive replication,resulting in recurrent diseases.Therefore,a better understanding of the stress-induced susceptibility to HSV-1 primary infection and reactivation is needed and will provide helpful insights into the effective control and treatment of HSV-1.Here we reviewed the recent advances in the studies of HSV-1 susceptibility,latency and reactivation.We included mechanisms involved in primary infection and the regulation of latency and described how stress-induced changes increase the susceptibility to primary and recurrent infections.
基金supported,in part,by National Natural Science Foundation of China(grant numbers 81673709,82004231,U1801284 and 81873209)Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(grant number 2017BT01Y036,China)and GDUPS(2019,China)+5 种基金Guangdong Science and Technology Foundation for Distinguished Young Scholars(grant number 2017A030306004,China)Science and Technology Program of Guangzhou(grant numbers 201903010062 and 202102010116,China)Fellowship of China Postdoctoral Science Foundation(grant number 2020M683204,China)Guangdong Basic and Applied Basic Research Fund(grant number 2020A1515110388,China)Fundamental Research Funds for the Central Universities(grant number 21620448,China)support of K.C.Wong Education Foundation。
文摘The relationship between chronic psychological stress and tumorigenesis has been well defined in epidemiological studies;however, the underlying mechanism remains underexplored. In this study, we discovered that impaired macrophage phagocytosis contributed to the psychological stressevoked tumor susceptibility, and the stress hormone glucocorticoid(GC) was identified as a principal detrimental factor. Mechanistically, GC disturbed the balance of the "eat me" signal receptor(low-density lipoprotein receptor-related protein-1, LRP1) and the "don’t eat me" signal receptor(signal regulatory protein alpha, SIRPa). Further analysis revealed that GC led to a direct, glucocorticoid receptor(GR)-dependent trans-repression of LRP1 expression, and the repressed LRP1, in turn, resulted in the elevatedgene level of SIRPa by down-regulating mi RNA-4695-3 p. These data collectively demonstrate that stress induces the imbalance of the LRP1/SIRPa axis and entails the disturbance of tumor cell clearance by macrophages. Our findings provide the mechanistic insight into psychological stress-evoked tumor susceptibility and indicate that the balance of LRP1/SIRPa axis may serve as a potential therapeutic strategy for tumor treatment.
基金This work was supported,in part,by National Key Research and Development Program of China(2017YFC1700400,2017YFC1700404,2022YFC0867400)Natural Science Foundation of China(82125038,81904068,81873209,U1801284,81903821,82174054)+3 种基金the Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(2017BT01 Y036)GDUPS(2019),Natural Science Foundation of Guangdong(2019A1515010909,2021A1515011297)Science and Technology Program of Guangzhou(201903010062,907158833068)the Innovation Team Project of Guangdong Provincial Department of Education(2020KCXTD003).
文摘Myocardial ischemia/reperfusion(/R)injury is a classic type of cardiovascular disease characterized by injury to cardiomyocytes leading to various forms of cell death.It is believed that irreversible myocardial damage resulted from I/R occurs due to oxidative stress evoked during the reperfusion phase.Here we demonstrate that ischemia triggers a specific redox reaction of polyunsaturated fatty acids(PUFA)-phospholipids in myocardial cells,which acts as a priming signaling that initiates the outbreak of robust oxidative damage in the reperfusion phase.Using animal and in vitro models,the crucial lipid species in I/R injury were identifed to be oxidized PUFAs enriched phosphatidylethanolamines.Using multi-omics,arachidonic acid 15-lipoxygenase-1(ALOx15)was identified as the primary mediator of ischemia-provoked phospholipid peroxidation,which was further confirmed using chemogenetic approaches.Collectively,our results reveal that ALOx15 induction in the ischemia phase acts as a"burning point"to ignite phospholipid oxidization into ferroptotic signals.This finding characterizes a novel molecular mechanism for myocardial ischemia injury and offers a potential therapeutic target for early intervention of V/R injury.
基金partly supported by National Natural Science Foundation of China(Grant Nos.U1801284,81573675,81622050,81873209 and 81673709)National Key Research and Development Program of China(Grant No.2017YFC1700404)+5 种基金the Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(Grant No.2017BT01Y036,China)GDUPS(2019,China)the Guangdong Science and Technology Foundation(Grant No.2017A030306004)the Program of Hong Kong Scholar(XJ2016017,China)Science and Technology Program of Guangzhou(Grant No.201903010062,China)the Youth Top-notch Talent Support Program of Guangdong Province(Grant No.2016TQ03R586,China)
文摘Herpes simplex virus type 1(HSV-1)is a ubiquitous and widespread human pathogen,which gives rise to a range of diseases,including cold sores,corneal blindness,and encephalitis.Currently,the use of nucleoside analogs,such as acyclovir and penciclovir,in treating HSV-1 infection often presents limitation due to their side effects and low efficacy for drug-resistance strains.Therefore,new anti-herpetic drugs and strategies should be urgently developed.Here,we reported that baicalein,a naturally derived compound widely used in Asian countries,strongly inhibited HSV-1 replication in several models.Baicalein was effective against the replication of both HSV-1/F and HSV-1/Blue(an acyclovir-resistant strain)in vitro.In the ocular inoculation mice model,baicalein markedly reduced in vivo HSV-1/F replication,receded inflammatory storm and attenuated histological changes in the cornea.Consistently,baicalein was found to reduce the mortality of mice,viral loads both in nose and trigeminal ganglia in HSV-1 intranasal infection model.Moreover,an ex vivo HSV-1-EGFP infection model established in isolated murine epidermal sheets confirmed that baicalein suppressed HSV-1 replication.Further investigations unraveled that dual mechanisms,inactivating viral particles and inhibiting IκB kinase beta(IKK-β)phosphorylation,were involved in the anti-HSV-1 effect of baicalein.Collectively,our findings identified baicalein as a promising therapy candidate against the infection of HSV-1,especially acyclovir-resistant strain.
文摘“Life is pleasant. Death is peaceful. It's the transition that's troublesome.”——Isaac Asimov Copper is an essential trace element central to cardiovascular, neuronal, and immune functions. While copper deficiency is generally rare, chronic or long-term copper exposure can result in toxicity.
基金supported,in part,by Natural Science Foundation of China(grant numbers 81622050,81573675,U1801284,81673709,81873209)National Key Research and Development Program of China(grant number 2017YFC1700404)+4 种基金the Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(grant number 2017BT01Y036)and GDUPS(2019)the Guangdong Science and Technology Foundation for Distinguished Young Scholars(grant number 2017A030306004)the Youth Top-notch Talent Support Program of Guangdong Province(2016TQ03R586)the Program of Hong Kong Scholar(XJ2016017)the Science and Technology Program of Guangzhou(grant number 201903010062).
文摘Although stress has been known to increase the susceptibility of pathogen infection,the underlying mechanism remains elusive.In this study,we reported that restraint stress dramatically enhanced the morbidity and mortality of mice infected with the influenza virus(H1N1)and obviously aggravated lung inflammation.Corticosterone(CORT),a main type of glucocorticoids in rodents,was secreted in the plasma of stressed mice.We further found that this stress hormone significantly boosted virus replication by restricting mitochondrial antiviral signaling(MAVS)protein-transduced IFN-βproduction without affecting its mRNA level,while the deficiency of MAVS abrogated stress/CORT-induced viral susceptibility in mice.Mechanistically,the effect of CORT was mediated by proteasome-dependent degradation of MAVS,thereby resulting in the impediment of MAVS-transduced IFN-βgeneration in vivo and in vitro.Furthermore,RNA-seq assay results indicated the involvement of Mitofusin 2(Mfn2)in this process.Gain-and loss-offunction experiments indicated that Mfn2 interacted with MAVS and recruited E3 ligase SYVN1 to promote the polyubiquitination of MAVS.Co-immunoprecipitation experiments clarified an interaction between any two regions of Mfn2(HR1),MAVS(C-terminal/TM)and SYVN1(TM).Collectively,our findings define the Mfn2-SYVN1 axis as a new signaling cascade for proteasome-dependent degradation of MAVS and a‘fine tuning’of antiviral innate immunity in response to influenza infection under stress.
基金financially supported by grants from the National Natural Science Foundation of China(81422054)the Guangdong Natural Science Funds for Distinguished Young Scholar(S2013050014287)+2 种基金Guangdong Special Support Program(2014TQ01R420)Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme(Hao Gao,2014)K.C.Wong Education Foundation(Hao Gao,2016)
文摘Four new dicaffeoylspermidine derivatives, lycibarbarspermidines P–S(1–4), were isolated from the fruit of Lycium barbarum(wolfberry).The structures were determined by extensive spectroscopic(HRESIMS, 1 D NMR, and 2 D NMR) analyses and chemical methods.Dicaffeoylspermidine derivatives are a kind of major bioactive and characteristic constituents in wolfberry, and the discovery of 1–4 added new members of this family.
基金supported, in part, by Natural Science Foundation of China (grant numbers 82125038 and 82004231)Natural Science Foundation of Guangdong (grant number 2021B1515120023, China)+3 种基金the Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program (grant number 2017BT01Y036, China)GDUPS (2019, China)the Innovation Team Project of Guangdong Provincial Department of Education (2020KCXTD003, China)Guangdong Basic and Applied Basic Research Fund (grant number 2020A1515110388, China)。
文摘Lipids are the major components of cells in the central nervous system(CNS) and are essential for the brain development and homeostasis. Several studies have demonstrated that impaired lipid metabolism is closely related to neurological diseases among the elderly^(1). Lipidomics can serve us well in identifying and quantifying lipid molecules in cells, tissues, and organisms at the system level. However, finding out biologically meaningful lipids from the massive lipidomics data is not straightforward.
基金This work was supported by the National Natural Science Foundation of China(No.82125038,82004231)Guangdong Basic and Applied Basic Research Fund(No.2020A1515110388,China)GDUPS(2019,China).
文摘It is commonly known that psychological stress affects immunity through the nervous system,but the specific stress networks between brain and peripheral leukocytes,or their relationship with disease initiation or progression have yet to been delineated.A recent study by Poller and his colleagues1 propose an innovative perspective that distinct brain circuits rapidly manipulate a large-scale whole-body re-distribution of leukocytes(neutrophils,monocytes,lymphocytes)during psychological stress.
