Neuroinflammation is the primary driver and signature of many neurodevelopmental disorders.However,because neurodevelopmental disorders caused by neuroinflammation are difficult to detect at the early stage,their prog...Neuroinflammation is the primary driver and signature of many neurodevelopmental disorders.However,because neurodevelopmental disorders caused by neuroinflammation are difficult to detect at the early stage,their progression remains unclear.To date,neither animal experiments nor in vitro models have uncovered their early developmental characteristics caused by neuroinflammation.In this study,we developed a neurovascular-unit-on-a-chip(NVU-on-a-chip)to model inflammation-induced neurodevelopmental disorders.With the chip,dynamic visualization of the progression caused by neuroinflammation was clearly demonstrated,and the changes in angiogenesis and neural differentiation under neuroinflammation were replicated.In addition,the activation of astrocytes and damage to neurons and capillaries at the early stage of neurodevelopmental disorders were observed.The results revealed for the first time the structural disruption of the neurovascular units and the neurovascular coupling failure caused by neuroinflammation.Furthermore,the outcomes of anti-inflammatory intervention using ibuprofen were preliminarily demonstrated.This work provides insights into the early progression of neurodevelopmental disorders caused by neuroinflammation and offers a platform for the development of therapeutic strategies for neuroinflammation.展开更多
On 22 May 2021,the Maduo Earthquake occurred on a branch fault of the East Kunlun fault in the Bayan Har Block,which provides opportunity to constrain fault geometry and strain accumulation and release for assessment ...On 22 May 2021,the Maduo Earthquake occurred on a branch fault of the East Kunlun fault in the Bayan Har Block,which provides opportunity to constrain fault geometry and strain accumulation and release for assessment of earthquake hazards.We processed the Sentinal-1A/B SAR images acquired before and after the earthquake,with which we constrained a finite fault model to best fit to the combined data set of downsampled InSAR image and GPS displacements.The inversion indicates that the Maduo event ruptured a 160 km long section striking 286.5°and a dipping 81.39°with rake angle of 4.62°.The model suggests three compact rupture areas with the slip amplitude exceeding 4 m on the main rupture section and the largest slip region is in the east of the epicenter with a slip of approximately 4.6 m below the surface,in a good agreement with the field geological survey.The total geodetic moment is 1.67×10^(20) N·m equivalent to Mw7.44,slightly larger than estimate of the USGS.展开更多
On 3 July 2015, a Mw 6.4 earthquake occurred on a blind fault struck Pishan, Xinjiang,China. By combining Crustal Movement Observation Network of China（CMONOC） and other Static Global Positioning System（GPS） sites...On 3 July 2015, a Mw 6.4 earthquake occurred on a blind fault struck Pishan, Xinjiang,China. By combining Crustal Movement Observation Network of China（CMONOC） and other Static Global Positioning System（GPS） sites surrounding Pishan region, it provides a rare chance for us to constrain the slip rupture for such a moderate event. The maximum displacement is up to 12 cm, 2 cm for coseismic and postseismic deformation, respectively,and both the deformation patterns show a same direction moving northeastward. With rectangular dislocation model, a magnitude of Mw6.48, Mw6.3 is calculated based on coseismic, postseismic deformation respectively. Our result indicates the western Kunlun range is still moving toward Tarim Basin followed by an obvious postseismic slip associated with this earthquake. To determine a more reasonable model for postseismic deformation, a longer GPS dataset will be needed.展开更多
AIM:To reveal whether and how Yes-associated protein(YAP)promotes the occurrence of subretinal fibrosis in agerelated macular degeneration(AMD).METHODS:Cobalt chloride(Co Cl2)was used in primary human umbilical vein e...AIM:To reveal whether and how Yes-associated protein(YAP)promotes the occurrence of subretinal fibrosis in agerelated macular degeneration(AMD).METHODS:Cobalt chloride(Co Cl2)was used in primary human umbilical vein endothelial cells(HUVECs)to induce hypoxia in vitro.Eight-week-old male C57 BL/6 J mice weighing 19-25 g were used for a choroidal neovascularization(CNV)model induced by laser photocoagulation in vivo.Expression levels of YAP,phosphorylated YAP,mesenchymal markers[αsmooth muscle actin(α-SMA),vimentin,and Snail],and endothelial cell markers(CD31 and zonula occludens 1)were measured by Western blotting,quantitative real-time PCR,and immunofluorescence microscopy.Small molecules YC-1(Lificiguat,a specific inhibitor of hypoxia-inducible factor 1α),CA3(CIL56,an inhibitor of YAP),and XMU-MP-1(an inhibitor of Hippo kinase MST1/2,which activates YAP)were used to explore the underlying mechanism.RESULTS:Co Cl2 increased expression of mesenchymal markers,decreased expression of endothelial cell markers,and enhanced the ability of primary HUVECs to proliferate and migrate.YC-1 suppressed hypoxia-induced endothelialto-mesenchymal transition(End MT).Moreover,hypoxia promoted total expression,inhibited phosphorylation,and enhanced the transcriptional activity of YAP.XMU-MP-1 enhanced hypoxia-induced End MT,whereas CA3 elicited the opposite effect.Expression of YAP,α-SMA,and vimentin were upregulated in the laser-induced CNV model.However,silencing of YAP by vitreous injection of small interfering RNA targeting YAP could reverse these changes.CONCLUSION:The findings reveal a critical role of the hypoxia-inducible factor-1α(HIF-1α)/YAP signaling axis in End MT and provide a new therapeutic target for treatment of subretinal fibrosis in AMD.展开更多
Objective This study aimed to analyze the clinical efficacy of the Jianpi Shengxue tablet for treating renal anemia.Methods A total of 200 patients with renal anemia from December 2020 to December 2022 were enrolled a...Objective This study aimed to analyze the clinical efficacy of the Jianpi Shengxue tablet for treating renal anemia.Methods A total of 200 patients with renal anemia from December 2020 to December 2022 were enrolled and randomly divided into two groups.Patients in the control group were treated with polysaccharide-iron complex,and those in the experimental group were administered Jianpi Shengxue tablet.After 8 weeks of continuous treatment,the therapeutic outcomes regarding anemia were compared between the two groups.Results After treatment,the red blood cell(RBC)count,hematocrit(HCT),reticulocyte percentage(RET),ferritin(SF),serum iron(SI),transferrin saturation(TSAT),and serum albumin(ALB)all increased(P<0.01),and the clinical symptom score and total iron binding capacity decreased(P<0.01)in the experimental group.Moreover,the improvements in RBC,HCT,RET,SF,SI,TAST,ALB,and clinical symptoms(fatigue,anorexia,dull skin complexion,numbness of hands and feet)in the experimental group were significantly greater than those in the control group(P<0.05).The total effective rate for treating renal anemia was significantly higher in the experimental group than in the control group(P<0.01).Conclusion The Jianpi Shengxue tablet demonstrates efficacy in treating renal anemia,leading to significant improvements in the laboratory examination results and clinical symptoms of patients with renal anemia.展开更多
Secondary sclerosing cholangitis(SSC)is a chronic cholestatic liver disease characterized by fibrosis and stricture of the bile ducts.SSC in association with multiple factors such as spontaneous choledochoduodenal fis...Secondary sclerosing cholangitis(SSC)is a chronic cholestatic liver disease characterized by fibrosis and stricture of the bile ducts.SSC in association with multiple factors such as spontaneous choledochoduodenal fistula and metastatic gallbladder cancer has rarely been reported.However,to the best of our knowledge,reports of SSC after percutaneous transhepatic biliary drainage(PTBD),especially in cases with diffuse calcification of the bile duct walls,have not been reported.We report a case of SSC from PTBD in a patient with gallbladder cancer after surgery.The patient underwent external percutaneous biliary drainage for malignant bile duct obstruction after cholecystectomy.Repeated exchanges were performed at the first and the sixth month after PTBD using an internal and external drainage catheter.Two months after the third catheter exchange,findings of laboratory and imaging examinations were suggestive of SSC.The liver function tests of the patient were suggestive of cholestasis.Multidetector computed tomography showed diffuse calcification of the bile duct walls.Cholangiography showed intrahepatic biliary stenosis or dilatation.展开更多
Sulfur-containing polymer(SCP)is considered as an outstanding cathode material for lithium-sulfur batteries.However,undesirable soluble polysulfides may shuttle in electrolyte,concluding long-chain Li_(2)S_(n)(n>4)...Sulfur-containing polymer(SCP)is considered as an outstanding cathode material for lithium-sulfur batteries.However,undesirable soluble polysulfides may shuttle in electrolyte,concluding long-chain Li_(2)S_(n)(n>4)and short-chain Li2Sn(n≤4),as well as the sluggish conversion kinetics are yet to be solved to enhance the performance of lithium-sulfur batteries.Here Se-doped sulfurized polyaniline with adjusted sulfur-chain-S_(x)-(x≤6)contribute to ensure the absence of long-chain polysulfides,and the skeleton with quinoid imine can endow strongly adsorption towards short-chain polysulfides by the reversible transition between deprotonated/protonated imine(-NH^(+)=and-N=),which offer double insurance against suppressing“shuttle effect”.Furthermore,Se atoms are doped into sulfurized polysulfides to accelerate the redox conversion and take a frontier orbital theory-oriented view into catalytic mechanism.Se-doped sulfurized polyaniline as active materials for lithium-organosulfur batteries delivers good electrochemical performance,including high rate,reversible specific capacity(680 mA h g^(-1)at 0.1 A g^(-1)),and lower capacity decay rate only of 0.15%with near 100%coulomb efficiency during long-term cycle.This work provides a valuable guiding ideology and promising solution for the chemistry-oriented structure design and practical application for lithium-organosulfur batteries.展开更多
Jatropha curcas seedlings were cultured for 21 d under 6 zinc concentrations of 0,25,50,100,200,400 mg/l to study the effects of ZnS O4 stress on the mechanism of resistance to heavy metal stress of J. curcas seedling...Jatropha curcas seedlings were cultured for 21 d under 6 zinc concentrations of 0,25,50,100,200,400 mg/l to study the effects of ZnS O4 stress on the mechanism of resistance to heavy metal stress of J. curcas seedlings. The results showed that with the increase of stress concentration,the content of chlorophyll-a decreased and the activity of POD first increased and then maintained at a certain level or decreased slowly. The contents of soluble sugar,malondialdehyde,soluble protein and free proline increased,and the root activity increased first and then decreased. The relative conductivity of J. curcas seedlings decreased first and then increased,which indicated that the leaves of J. curcas seedlings had some adaptability and self-repairing capability under zinc toxicity stress,but the adaptability and self-repair ability were limited.展开更多
Click chemistry is a rapid,reliable,and powerful function and a highly selective organic reaction that facilitates the efficient synthesis of various molecules by joining small units.This approach has found widespread...Click chemistry is a rapid,reliable,and powerful function and a highly selective organic reaction that facilitates the efficient synthesis of various molecules by joining small units.This approach has found widespread applications in fields such as drug development,chemical synthesis,and molecular biology.In recent years,the reaction of alkali-catalyzed polymerization of thiol and sulfur has been employed to prepare various sulfur-containing polymers,which are applied as electrochemical active electrode materials in the pursuit of good performance.In this study,it is surprising to find that the reaction mechanism exhibits characteristics of both the alkali-catalyzed sulfhydryl Micheal addition reaction and thiol-epoxy click chemistry;for the first time,thiol-sulfur click chemistry is defined in detail,providing a comprehensive description of its underlying scientific principles.The introduction of this new reaction pathway holds significant potential for advancing research and the development of sulfur-containing polymers.Based on this novel click chemistry,a new sulfur-containing polymer,polydivinylthioether hexasulfide,has been designed and successfully applied as a cathode material in lithium-organosulfide batteries.This material demonstrates excellent electrochemical performance,achieving an initial capacity that reaches 790.5 mAh g−1(82.6%of theoretical capacity),and in a long-term cycle test,the capacity decay rate is only 0.063%after 1,000 cycles.展开更多
Mitochondrial metabolism-regulated epigenetic modification is a driving force of aging and a promising target for therapeutic intervention.Mitochondrial malate dehydrogenase(MDH2),an enzyme in the TCA cycle,was identi...Mitochondrial metabolism-regulated epigenetic modification is a driving force of aging and a promising target for therapeutic intervention.Mitochondrial malate dehydrogenase(MDH2),an enzyme in the TCA cycle,was identified as an anti-aging target through activity-based protein profiling in present study.The expression level of MDH2 was positively correlated with the cellular senescence in Mdh2 knockdown or overexpression fibroblasts.Glibenclamide(Gli),a classic anti-glycemic drug,was found to inhibit the activity of MDH2 and relieve fibroblast senescence in an MDH2-dependent manner.The anti-aging effects of Gli were also further validated in vivo,as it extended the lifespan and reduced the frailty index of naturally aged mice.Liver specific Mdh2 knockdown eliminated Gli’s beneficial effects in naturally aged mice,reducing p^(16INK4a) expression and hepatic fibrosis.Mechanistically,MDH2 inhibition or knockdown disrupted central carbon metabolism,then enhanced the methionine cycle flux,and subsequently promoted histone methylation.Notably,the tri-methylation of H3K27,identified as a crucial methylation site in reversing cellular senescence,was significantly elevated in hepatic tissues of naturally aged mice with Mdh2 knockdown.Taken together,these findings reveal that MDH2 inhibition or knockdown delays the aging process through metabolic-epigenetic regulation.Our research not only identified MDH2 as a potential therapeutic target and Gli as a lead compound for anti-aging drug development,but also shed light on the intricate interplay of metabolism and epigenetic modifications in aging.展开更多
Breakage rate is one of the most important indicators to evaluate the harvesting performance of a combine harvester.It is affected by operating parameters of a combine such as feeding rate,the peripheral speed of the ...Breakage rate is one of the most important indicators to evaluate the harvesting performance of a combine harvester.It is affected by operating parameters of a combine such as feeding rate,the peripheral speed of the threshing cylinder and concave clearance,and shows complex non-linear law.Real-time acquisition of the breakage rate is an effective way to find the correlation of them.In addition,real-time monitoring of the breakage rate can help the driver optimize and adjust the operating parameters of a combine harvester to avoid the breakage rate exceeding the standard.In this study,a real-time monitoring method for the grain breakage rate of the rice combine harvester based on machine vision was proposed.The structure of the sampling device was designed to obtain rice kernel images of high quality in the harvesting process.According to the working characteristics of the combine,the illumination and installation of the light source were optimized,and the lateral lighting system was constructed.A two-step method of“color training-verification”was applied to identify the whole and broken kernels.In the first step,the local threshold algorithm was used to get the edge of kernel particles in a few training images with binary transformation,extract the color spectrum of each particle in color-space HSL and output the recognition model file.The second step was to verify the recognition accuracy and the breakage rate monitoring accuracy through grabbing and processing images in the laboratory.The experiments of about 2300 particles showed that the recognition accuracy of 96%was attained,and the monitoring values of breakage rate and the true artificial monitoring values had good trend consistency.The monitoring device of grain breakage rate based on machine vision can provide technical supports for the intellectualization of combine harvester.展开更多
Condition monitoring ensures the safety of freight railroad operations. With the development of machine vision technology, visual inspection has become a principal means of condition monitoring. The brake shoe key （...Condition monitoring ensures the safety of freight railroad operations. With the development of machine vision technology, visual inspection has become a principal means of condition monitoring. The brake shoe key （BSK） is an important component in the brake system, and its absence will lead to serious accidents. This paper presents a novel method for automated visual inspection of the BSK condition in freight cars. BSK images are first acquired by hardware devices. The subsequent in- spection process is divided into three stages： first, the region-of-interest （ROI） is segmented from the source image by an im- proved spatial pyramid matching scheme based on multi-scale census transform （MSCT）. To localize the BSK in the ROI, cen- sus transform （CT） on gradient images is developed in the second stage. Then gradient encoding histogram （GEH） features and linear support vector machines （SVMs） are used to generate a BSK localization classifier. In the last stage, a condition classifier is trained by SVM, but the features are extracted from gray images. Finally, the ROI, BSK localization, and condition classifiers are cascaded to realize a completely automated inspection system. Experimental results show that the system achieves a correct inspection rate of 99.2% and a speed of 5 frames/s, which represents a good real-time performance and high recognition accuracy.展开更多
For a long time,trouble detection and maintenance of freight cars have been completed manually by inspectors.To realize the transition from manual to computer-based detection and maintenance,we focus on dust collector...For a long time,trouble detection and maintenance of freight cars have been completed manually by inspectors.To realize the transition from manual to computer-based detection and maintenance,we focus on dust collector localization under complex conditions in the trouble of moving freight car detection system.Using mid-level features which are also named flexible edge arrangement(FEA) features,we first build the edge-based 2D model of the dust collectors,and then match target objects by a weighted Hausdorff distance method.The difference is that the constructed weighting function is generated by the FEA features other than specified subjectively,which can truly reflect the most basic property regions of the 3D object.Experimental results indicate that the proposed algorithm has better robustness to variable lighting,different viewing angle,and complex texture,and it shows a stronger adaptive performance.The localization correct rate of the target object is over 90%,which completely meets the need of practical applications.展开更多
Interventional radiology （IR） is a clinical medical specialty which uses minimally invasive image-guided procedures to diagnose and treat disease.[1] IR was introduced in China during the 1980s. It is a rapidly expa...Interventional radiology （IR） is a clinical medical specialty which uses minimally invasive image-guided procedures to diagnose and treat disease.[1] IR was introduced in China during the 1980s. It is a rapidly expanding field and has become an integral part of medical care. The Chinese Society of Interventional Radiology is one of the largest societies for IR in the world.[2] However, there is no independent IR curriculum among clinical undergraduate programs in China, which is not helpful for clinical medical undergraduates to improve their cognition for IR. In this article, we report our initial experience of the IR teaching curriculum and discuss whether awareness and interest of IR could be improved by a systematic study of IR curriculum among clinical medical undergraduates.展开更多
Besides lithium-ion batteries, it is imperative to develop new battery energystorage system with high energy density. In conjunction with the developmentof Li-S batteries, emerging sulfur-containing polymers with tuna...Besides lithium-ion batteries, it is imperative to develop new battery energystorage system with high energy density. In conjunction with the developmentof Li-S batteries, emerging sulfur-containing polymers with tunable sulfur-chain length and organic groups gradually attract much attention as cathodematerials. This can avoid the problems that are impeding the development ofthe typical Li-S batteries, such as volume expansion, active material dissolu-tion, shuttle effect, and so on. This review aims to generalize the type ofsulfur-containing polymers and the working principles in Li-S batteries. Thesulfur-containing polymers (R-Sn-R) with different sulfur-chain length (n > 6,n ≤ 2, and 3 ≤ n ≤ 6) are summarized. It also discusses several organic groupssuch as phenyl rings, N-heterocycles, and unique structure with cross-linkednetworks and multi-micropores skeleton. This review also explores other strat-egies of sulfur-containing polymers in the rest of Li-S batteries, providing asummary of the advantages of sulfur-containing polymers, recent develop-ment, in-depth discussion of the mechanism in Li-S batteries, and organicgroup-structure-performance relationship. This review would have guidelinesfor future development of sulfur-containing polymers in Li-S batteries.展开更多
The development of adaptive emotion regulation(ER)plays a pivotal role in adolescent mental health and socio-emotional adaptation.Dispositional optimism,as an important protective factor for adolescent adjustment,may ...The development of adaptive emotion regulation(ER)plays a pivotal role in adolescent mental health and socio-emotional adaptation.Dispositional optimism,as an important protective factor for adolescent adjustment,may affect adolescent ER and subsequently influence adaptive outcomes.In this review,the changes and challenges,the role of ER in socio-emotional adjustment,and the developmental characteristics of implicit and explicit ER during adolescence are described.Subsequently,by employing the top-down model of personality,coping,and emotion,how dispositional optimism may affect psychological adjustment from the perspective of ER is analyzed.Furthermore,how the differences in adolescents’dispositional optimism may be reflected by the differences in implicit ER is discussed.Finally,recommendations for future research are outlined.展开更多
基金supported by the National Key R&D Program of China(No.2018AAA0100300)the National Natural Science Foundation of China(Nos.82072018,82274375,and 82402490)+5 种基金the Anhui Provincial Science and Technology Major Project(No.202203a07020006)the Strategic Priority Research Program(C)of the Chinese Academy of Sciences(CAS)(No.XDC07040200)the Key R&D Program of Anhui Province(No.2022e07020012)the Natural Science Foundation of Anhui Province(No.2208085QH256)the Fundamental Research Funds for the Central Universities(No.WK2100000042)the China Postdoctoral Science Foundation(No.2022M713055).
文摘Neuroinflammation is the primary driver and signature of many neurodevelopmental disorders.However,because neurodevelopmental disorders caused by neuroinflammation are difficult to detect at the early stage,their progression remains unclear.To date,neither animal experiments nor in vitro models have uncovered their early developmental characteristics caused by neuroinflammation.In this study,we developed a neurovascular-unit-on-a-chip(NVU-on-a-chip)to model inflammation-induced neurodevelopmental disorders.With the chip,dynamic visualization of the progression caused by neuroinflammation was clearly demonstrated,and the changes in angiogenesis and neural differentiation under neuroinflammation were replicated.In addition,the activation of astrocytes and damage to neurons and capillaries at the early stage of neurodevelopmental disorders were observed.The results revealed for the first time the structural disruption of the neurovascular units and the neurovascular coupling failure caused by neuroinflammation.Furthermore,the outcomes of anti-inflammatory intervention using ibuprofen were preliminarily demonstrated.This work provides insights into the early progression of neurodevelopmental disorders caused by neuroinflammation and offers a platform for the development of therapeutic strategies for neuroinflammation.
文摘On 22 May 2021,the Maduo Earthquake occurred on a branch fault of the East Kunlun fault in the Bayan Har Block,which provides opportunity to constrain fault geometry and strain accumulation and release for assessment of earthquake hazards.We processed the Sentinal-1A/B SAR images acquired before and after the earthquake,with which we constrained a finite fault model to best fit to the combined data set of downsampled InSAR image and GPS displacements.The inversion indicates that the Maduo event ruptured a 160 km long section striking 286.5°and a dipping 81.39°with rake angle of 4.62°.The model suggests three compact rupture areas with the slip amplitude exceeding 4 m on the main rupture section and the largest slip region is in the east of the epicenter with a slip of approximately 4.6 m below the surface,in a good agreement with the field geological survey.The total geodetic moment is 1.67×10^(20) N·m equivalent to Mw7.44,slightly larger than estimate of the USGS.
基金supported by National Natural Science Foundation of China(41304014,41204001,41274037 and 41431069)National 863 Project of China(2013AA122501)+4 种基金China postdoctoral science foundation(2015M57228)the Basic Fund of Hubei Subsurface Multi-scale Imaging Key Laboratory,Institute of Geophysics and Geomatics,China University of Geosciences,Wuhan(SMIL-2015-01)the Fundamental Research Funds for National Universities(CUGL150810)China Scholarship Council(201506415072)the Basic Research Fund of Key Laboratory of Geospace Environment and Geodesy,Ministry of Education of China(13-02-11 and 14-01-01)
文摘On 3 July 2015, a Mw 6.4 earthquake occurred on a blind fault struck Pishan, Xinjiang,China. By combining Crustal Movement Observation Network of China（CMONOC） and other Static Global Positioning System（GPS） sites surrounding Pishan region, it provides a rare chance for us to constrain the slip rupture for such a moderate event. The maximum displacement is up to 12 cm, 2 cm for coseismic and postseismic deformation, respectively,and both the deformation patterns show a same direction moving northeastward. With rectangular dislocation model, a magnitude of Mw6.48, Mw6.3 is calculated based on coseismic, postseismic deformation respectively. Our result indicates the western Kunlun range is still moving toward Tarim Basin followed by an obvious postseismic slip associated with this earthquake. To determine a more reasonable model for postseismic deformation, a longer GPS dataset will be needed.
基金National Natural Science Foundation of China(No.81970817,No.81873680)。
文摘AIM:To reveal whether and how Yes-associated protein(YAP)promotes the occurrence of subretinal fibrosis in agerelated macular degeneration(AMD).METHODS:Cobalt chloride(Co Cl2)was used in primary human umbilical vein endothelial cells(HUVECs)to induce hypoxia in vitro.Eight-week-old male C57 BL/6 J mice weighing 19-25 g were used for a choroidal neovascularization(CNV)model induced by laser photocoagulation in vivo.Expression levels of YAP,phosphorylated YAP,mesenchymal markers[αsmooth muscle actin(α-SMA),vimentin,and Snail],and endothelial cell markers(CD31 and zonula occludens 1)were measured by Western blotting,quantitative real-time PCR,and immunofluorescence microscopy.Small molecules YC-1(Lificiguat,a specific inhibitor of hypoxia-inducible factor 1α),CA3(CIL56,an inhibitor of YAP),and XMU-MP-1(an inhibitor of Hippo kinase MST1/2,which activates YAP)were used to explore the underlying mechanism.RESULTS:Co Cl2 increased expression of mesenchymal markers,decreased expression of endothelial cell markers,and enhanced the ability of primary HUVECs to proliferate and migrate.YC-1 suppressed hypoxia-induced endothelialto-mesenchymal transition(End MT).Moreover,hypoxia promoted total expression,inhibited phosphorylation,and enhanced the transcriptional activity of YAP.XMU-MP-1 enhanced hypoxia-induced End MT,whereas CA3 elicited the opposite effect.Expression of YAP,α-SMA,and vimentin were upregulated in the laser-induced CNV model.However,silencing of YAP by vitreous injection of small interfering RNA targeting YAP could reverse these changes.CONCLUSION:The findings reveal a critical role of the hypoxia-inducible factor-1α(HIF-1α)/YAP signaling axis in End MT and provide a new therapeutic target for treatment of subretinal fibrosis in AMD.
基金financially supported by the National Natural Science Foundation of China(No.82170701).
文摘Objective This study aimed to analyze the clinical efficacy of the Jianpi Shengxue tablet for treating renal anemia.Methods A total of 200 patients with renal anemia from December 2020 to December 2022 were enrolled and randomly divided into two groups.Patients in the control group were treated with polysaccharide-iron complex,and those in the experimental group were administered Jianpi Shengxue tablet.After 8 weeks of continuous treatment,the therapeutic outcomes regarding anemia were compared between the two groups.Results After treatment,the red blood cell(RBC)count,hematocrit(HCT),reticulocyte percentage(RET),ferritin(SF),serum iron(SI),transferrin saturation(TSAT),and serum albumin(ALB)all increased(P<0.01),and the clinical symptom score and total iron binding capacity decreased(P<0.01)in the experimental group.Moreover,the improvements in RBC,HCT,RET,SF,SI,TAST,ALB,and clinical symptoms(fatigue,anorexia,dull skin complexion,numbness of hands and feet)in the experimental group were significantly greater than those in the control group(P<0.05).The total effective rate for treating renal anemia was significantly higher in the experimental group than in the control group(P<0.01).Conclusion The Jianpi Shengxue tablet demonstrates efficacy in treating renal anemia,leading to significant improvements in the laboratory examination results and clinical symptoms of patients with renal anemia.
文摘Secondary sclerosing cholangitis(SSC)is a chronic cholestatic liver disease characterized by fibrosis and stricture of the bile ducts.SSC in association with multiple factors such as spontaneous choledochoduodenal fistula and metastatic gallbladder cancer has rarely been reported.However,to the best of our knowledge,reports of SSC after percutaneous transhepatic biliary drainage(PTBD),especially in cases with diffuse calcification of the bile duct walls,have not been reported.We report a case of SSC from PTBD in a patient with gallbladder cancer after surgery.The patient underwent external percutaneous biliary drainage for malignant bile duct obstruction after cholecystectomy.Repeated exchanges were performed at the first and the sixth month after PTBD using an internal and external drainage catheter.Two months after the third catheter exchange,findings of laboratory and imaging examinations were suggestive of SSC.The liver function tests of the patient were suggestive of cholestasis.Multidetector computed tomography showed diffuse calcification of the bile duct walls.Cholangiography showed intrahepatic biliary stenosis or dilatation.
基金partly supported by the National Natural Science Foundation of China(51763014 and 52073133)the Key Talent Project Foundation of Gansu Province+3 种基金Joint fund between Shenyang National Laboratory for Materials Science and State Key Laboratory of Advanced Processing and Recycling of Nonferrous Metals(18LHPY002)the Incubation Program of Excellent Doctoral Dissertation-Lanzhou University of Technologyexcellent doctoral Program of Gansu Province(22JR5RA240)the Program for Hongliu Distinguished Young Scholars in Lanzhou University of Technology。
文摘Sulfur-containing polymer(SCP)is considered as an outstanding cathode material for lithium-sulfur batteries.However,undesirable soluble polysulfides may shuttle in electrolyte,concluding long-chain Li_(2)S_(n)(n>4)and short-chain Li2Sn(n≤4),as well as the sluggish conversion kinetics are yet to be solved to enhance the performance of lithium-sulfur batteries.Here Se-doped sulfurized polyaniline with adjusted sulfur-chain-S_(x)-(x≤6)contribute to ensure the absence of long-chain polysulfides,and the skeleton with quinoid imine can endow strongly adsorption towards short-chain polysulfides by the reversible transition between deprotonated/protonated imine(-NH^(+)=and-N=),which offer double insurance against suppressing“shuttle effect”.Furthermore,Se atoms are doped into sulfurized polysulfides to accelerate the redox conversion and take a frontier orbital theory-oriented view into catalytic mechanism.Se-doped sulfurized polyaniline as active materials for lithium-organosulfur batteries delivers good electrochemical performance,including high rate,reversible specific capacity(680 mA h g^(-1)at 0.1 A g^(-1)),and lower capacity decay rate only of 0.15%with near 100%coulomb efficiency during long-term cycle.This work provides a valuable guiding ideology and promising solution for the chemistry-oriented structure design and practical application for lithium-organosulfur batteries.
基金Supported by the Special Fund for Scientific and Technological Base and Talents in Guangxi(Guike AD17129022)the Science and Technology Achievement Transformation and Promotion Project of Guilin City(20140115-1)+3 种基金the Key Science and Technology Research Project of Liuzhou City(2016B050202)the Project of Guangxi Education Department(201012MS189)the Fund for the Director of the Key Laboratory of Plant Functional Substance Research and Utilization of Guangxi(ZRJJ2016-20)the Natural Science Foundation of Guangxi(2017GXNSFBA198011)
文摘Jatropha curcas seedlings were cultured for 21 d under 6 zinc concentrations of 0,25,50,100,200,400 mg/l to study the effects of ZnS O4 stress on the mechanism of resistance to heavy metal stress of J. curcas seedlings. The results showed that with the increase of stress concentration,the content of chlorophyll-a decreased and the activity of POD first increased and then maintained at a certain level or decreased slowly. The contents of soluble sugar,malondialdehyde,soluble protein and free proline increased,and the root activity increased first and then decreased. The relative conductivity of J. curcas seedlings decreased first and then increased,which indicated that the leaves of J. curcas seedlings had some adaptability and self-repairing capability under zinc toxicity stress,but the adaptability and self-repair ability were limited.
基金supported by the National Natural Science Foundation of China(52463013 and 52073133)the Major Science and Technology Project of Gansu Province(22ZD6GA008)the Incubation Program of Excelent Doctoral Dissertation-Lanzhou Univer-sity of Technology.
文摘Click chemistry is a rapid,reliable,and powerful function and a highly selective organic reaction that facilitates the efficient synthesis of various molecules by joining small units.This approach has found widespread applications in fields such as drug development,chemical synthesis,and molecular biology.In recent years,the reaction of alkali-catalyzed polymerization of thiol and sulfur has been employed to prepare various sulfur-containing polymers,which are applied as electrochemical active electrode materials in the pursuit of good performance.In this study,it is surprising to find that the reaction mechanism exhibits characteristics of both the alkali-catalyzed sulfhydryl Micheal addition reaction and thiol-epoxy click chemistry;for the first time,thiol-sulfur click chemistry is defined in detail,providing a comprehensive description of its underlying scientific principles.The introduction of this new reaction pathway holds significant potential for advancing research and the development of sulfur-containing polymers.Based on this novel click chemistry,a new sulfur-containing polymer,polydivinylthioether hexasulfide,has been designed and successfully applied as a cathode material in lithium-organosulfide batteries.This material demonstrates excellent electrochemical performance,achieving an initial capacity that reaches 790.5 mAh g−1(82.6%of theoretical capacity),and in a long-term cycle test,the capacity decay rate is only 0.063%after 1,000 cycles.
基金the National Natural Science Foundation of China(grants 32121005 to J.L.,22277028 to Z.L.H.,22437002 to J.L,22467010 to B.L.L.,and 22207026 to W.W.L.)the Innovation Program of Shanghai Municipal Education Commission(grant 2021-01-07-00-02-E00104 to J.L.)+3 种基金the Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism(grant 2021 Sci&Tech 03-28 to J.L.)the Innovative Research Team of High-level Local Universities in Shanghai(grant SHSMU-ZDCX20212702 to J.L.)the Chinese Special Fund for State Key Laboratory of Bioreactor Engineering(2060204 to J.L.)the innovative research project of graduate students in Hainan Province(SA2400003375 to L.S.)。
文摘Mitochondrial metabolism-regulated epigenetic modification is a driving force of aging and a promising target for therapeutic intervention.Mitochondrial malate dehydrogenase(MDH2),an enzyme in the TCA cycle,was identified as an anti-aging target through activity-based protein profiling in present study.The expression level of MDH2 was positively correlated with the cellular senescence in Mdh2 knockdown or overexpression fibroblasts.Glibenclamide(Gli),a classic anti-glycemic drug,was found to inhibit the activity of MDH2 and relieve fibroblast senescence in an MDH2-dependent manner.The anti-aging effects of Gli were also further validated in vivo,as it extended the lifespan and reduced the frailty index of naturally aged mice.Liver specific Mdh2 knockdown eliminated Gli’s beneficial effects in naturally aged mice,reducing p^(16INK4a) expression and hepatic fibrosis.Mechanistically,MDH2 inhibition or knockdown disrupted central carbon metabolism,then enhanced the methionine cycle flux,and subsequently promoted histone methylation.Notably,the tri-methylation of H3K27,identified as a crucial methylation site in reversing cellular senescence,was significantly elevated in hepatic tissues of naturally aged mice with Mdh2 knockdown.Taken together,these findings reveal that MDH2 inhibition or knockdown delays the aging process through metabolic-epigenetic regulation.Our research not only identified MDH2 as a potential therapeutic target and Gli as a lead compound for anti-aging drug development,but also shed light on the intricate interplay of metabolism and epigenetic modifications in aging.
基金This research was supported by the National Key Research and Development Program of China(2016YFD0702001)the Key Research and Development Program of Jiangsu Province(BE2017358)+2 种基金the Graduate Innovative Projects of Jiangsu Province 2016(KYLX16_0879)the Anhui Natural Science Foundation(1608085ME112)and the Jiangsu Province Graduate Research and Practice Innovation Program(SJCX19_0550).
文摘Breakage rate is one of the most important indicators to evaluate the harvesting performance of a combine harvester.It is affected by operating parameters of a combine such as feeding rate,the peripheral speed of the threshing cylinder and concave clearance,and shows complex non-linear law.Real-time acquisition of the breakage rate is an effective way to find the correlation of them.In addition,real-time monitoring of the breakage rate can help the driver optimize and adjust the operating parameters of a combine harvester to avoid the breakage rate exceeding the standard.In this study,a real-time monitoring method for the grain breakage rate of the rice combine harvester based on machine vision was proposed.The structure of the sampling device was designed to obtain rice kernel images of high quality in the harvesting process.According to the working characteristics of the combine,the illumination and installation of the light source were optimized,and the lateral lighting system was constructed.A two-step method of“color training-verification”was applied to identify the whole and broken kernels.In the first step,the local threshold algorithm was used to get the edge of kernel particles in a few training images with binary transformation,extract the color spectrum of each particle in color-space HSL and output the recognition model file.The second step was to verify the recognition accuracy and the breakage rate monitoring accuracy through grabbing and processing images in the laboratory.The experiments of about 2300 particles showed that the recognition accuracy of 96%was attained,and the monitoring values of breakage rate and the true artificial monitoring values had good trend consistency.The monitoring device of grain breakage rate based on machine vision can provide technical supports for the intellectualization of combine harvester.
基金supported by the Special-Funded Programme on National Key Scientific Instruments and Equipment Development(No.2012YQ140032)the National Natural Science Foundation of China(No.51179076)+2 种基金the Jiangsu Province Postdoctoral Research Funding Plan(No.1402012B)the Scientific Research Foundation of Jiangsu University for Senior Personnel(No.14JDG134)the Jiangsu Province Science and Technology Support Plan(Industrial)(No.BE2012149)
文摘Condition monitoring ensures the safety of freight railroad operations. With the development of machine vision technology, visual inspection has become a principal means of condition monitoring. The brake shoe key （BSK） is an important component in the brake system, and its absence will lead to serious accidents. This paper presents a novel method for automated visual inspection of the BSK condition in freight cars. BSK images are first acquired by hardware devices. The subsequent in- spection process is divided into three stages： first, the region-of-interest （ROI） is segmented from the source image by an im- proved spatial pyramid matching scheme based on multi-scale census transform （MSCT）. To localize the BSK in the ROI, cen- sus transform （CT） on gradient images is developed in the second stage. Then gradient encoding histogram （GEH） features and linear support vector machines （SVMs） are used to generate a BSK localization classifier. In the last stage, a condition classifier is trained by SVM, but the features are extracted from gray images. Finally, the ROI, BSK localization, and condition classifiers are cascaded to realize a completely automated inspection system. Experimental results show that the system achieves a correct inspection rate of 99.2% and a speed of 5 frames/s, which represents a good real-time performance and high recognition accuracy.
基金Project supported by the National Natural Science Foundation of China (No. 61072134)the Research Fund for the Doctoral Program of Higher Education of China (No. 20101102110033)
文摘For a long time,trouble detection and maintenance of freight cars have been completed manually by inspectors.To realize the transition from manual to computer-based detection and maintenance,we focus on dust collector localization under complex conditions in the trouble of moving freight car detection system.Using mid-level features which are also named flexible edge arrangement(FEA) features,we first build the edge-based 2D model of the dust collectors,and then match target objects by a weighted Hausdorff distance method.The difference is that the constructed weighting function is generated by the FEA features other than specified subjectively,which can truly reflect the most basic property regions of the 3D object.Experimental results indicate that the proposed algorithm has better robustness to variable lighting,different viewing angle,and complex texture,and it shows a stronger adaptive performance.The localization correct rate of the target object is over 90%,which completely meets the need of practical applications.
文摘Interventional radiology （IR） is a clinical medical specialty which uses minimally invasive image-guided procedures to diagnose and treat disease.[1] IR was introduced in China during the 1980s. It is a rapidly expanding field and has become an integral part of medical care. The Chinese Society of Interventional Radiology is one of the largest societies for IR in the world.[2] However, there is no independent IR curriculum among clinical undergraduate programs in China, which is not helpful for clinical medical undergraduates to improve their cognition for IR. In this article, we report our initial experience of the IR teaching curriculum and discuss whether awareness and interest of IR could be improved by a systematic study of IR curriculum among clinical medical undergraduates.
基金Program for Hongliu Distinguished Young Scholars in Lanzhou University of TechnologyState Key Laboratory of Advanced Processing and Recycling of Nonferrous Metals,Grant/Award Number:18LHPY002+1 种基金Shenyang National Laboratory for Materials ScienceNational Natural Science Foundation of China,Grant/Award Numbers:52073133,51763014。
文摘Besides lithium-ion batteries, it is imperative to develop new battery energystorage system with high energy density. In conjunction with the developmentof Li-S batteries, emerging sulfur-containing polymers with tunable sulfur-chain length and organic groups gradually attract much attention as cathodematerials. This can avoid the problems that are impeding the development ofthe typical Li-S batteries, such as volume expansion, active material dissolu-tion, shuttle effect, and so on. This review aims to generalize the type ofsulfur-containing polymers and the working principles in Li-S batteries. Thesulfur-containing polymers (R-Sn-R) with different sulfur-chain length (n > 6,n ≤ 2, and 3 ≤ n ≤ 6) are summarized. It also discusses several organic groupssuch as phenyl rings, N-heterocycles, and unique structure with cross-linkednetworks and multi-micropores skeleton. This review also explores other strat-egies of sulfur-containing polymers in the rest of Li-S batteries, providing asummary of the advantages of sulfur-containing polymers, recent develop-ment, in-depth discussion of the mechanism in Li-S batteries, and organicgroup-structure-performance relationship. This review would have guidelinesfor future development of sulfur-containing polymers in Li-S batteries.
基金supported by grants from the National Natural Science Foundation of China(Grant No.31971018)Institute of Psychology,Chinese Academy of Sciences(Grant No.GJ202001)+2 种基金Educational Science Planning Project of Hubei Province(Grant No.2020GB056)the Research Fund Project(Grant No.2021Z01)the East Lake Scholar Program of Wuhan Sports University,China(Period:July 2017-July 2022)
文摘The development of adaptive emotion regulation(ER)plays a pivotal role in adolescent mental health and socio-emotional adaptation.Dispositional optimism,as an important protective factor for adolescent adjustment,may affect adolescent ER and subsequently influence adaptive outcomes.In this review,the changes and challenges,the role of ER in socio-emotional adjustment,and the developmental characteristics of implicit and explicit ER during adolescence are described.Subsequently,by employing the top-down model of personality,coping,and emotion,how dispositional optimism may affect psychological adjustment from the perspective of ER is analyzed.Furthermore,how the differences in adolescents’dispositional optimism may be reflected by the differences in implicit ER is discussed.Finally,recommendations for future research are outlined.
